Difference between revisions of "Team:ANU-Canberra/labbook"
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− | <h2>Our | + | <h2>Our Lab Book</h2> |
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− | <h3> | + | <h3>Blue light source</h3> |
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− | <p> | + | <p>We have devised a simple method to create your own light source from cheap, readily accessible materials, that can be customised for light intensity and pulse frequency - with minimal prior knowledge of electric circuits or experience with building them. |
+ | </p> | ||
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− | <h3> | + | <h3>Cre-recombinase functionality assay</h3> |
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− | <p>We | + | <p>We tested the functionality of the light-activated CRY2/CIB1-Cre-recombinase system using a reporter loxP construct. |
+ | </p> | ||
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− | <h3> | + | <h3>FRET Tests</h3> |
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− | <p> | + | <p>As a proof of principle for the expressability and association of the CRY2-CIB1 optogenetic system, we sought to conduct a fluorescence assay based upon the Forster resonance energy transfer (FRET) phenomenon.</p> |
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− | <h3> | + | <h3>Solubility Tests</h3> |
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− | <p> | + | <p>Even a successfully expressed protein may not function properly - solubility of our product was one indicator of how successful our light-inducible system would be.</p> |
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− | <h3> | + | <h3>Lab Notes</h3> |
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− | <p> | + | <p>A documentation of experiments we have run from the beginning to the end of our project.</p> |
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Latest revision as of 13:53, 18 September 2015
Our Lab Book
Blue light source
We have devised a simple method to create your own light source from cheap, readily accessible materials, that can be customised for light intensity and pulse frequency - with minimal prior knowledge of electric circuits or experience with building them.