Difference between revisions of "Team:CU Boulder/Composite Part"

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<h2> Composite Parts</h2>
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<h4>Note</h4>
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<p>In order to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best New Composite Part award</a>, you must fill out this page. Please give links to the Registry entries for the Composite parts you have made. Please see the Registry's <a href="http://parts.igem.org/Help:Parts#Basic_and_Composite_Parts"> Help:Parts page</a> for more information on part types.</p>
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<h1>Composite Parts</h1>
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<h2><a href="http://parts.igem.org/Part:BBa_K1718001">BBa_K1718001:</a></h2>
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<p><b>RFP under arabinose inducible pBad</b></p>
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<p>This part has a Red Fluorescent Protein (RFP) downstream of the pBad promoter. Expression is stimulated by L-arabinose. We used this part to test the leakiness of this promoter.</p>
 
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
 
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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<h2><a href="http://parts.igem.org/Part:BBa_K1718003">BBa_K1718003:</a></h2>
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<p><b>RFP under the LuxPr promoter</b></p>
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<p>In the presence of AHL, the LuxR transcription factor will bind AHL and activate the LuxPr promoter. RFP is downstream and will be expressed.</p>
  
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<h2><a href="http://parts.igem.org/Part:BBa_K1718004">BBa_K1718004:</a></h2>
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<p><b>LuxI and GFP</b></p>
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<p>LuxI leads to the production of AHL which, when in the presence of AHL, activates the LuxPr promoter. Downstream of the LuxI coding region is GFP, a reporter</p>
  
 
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<h2><a href="http://parts.igem.org/Part:BBa_K1718006">BBa_K1718006:</a></h2>
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<p><b>Genetic switch for Bxb1 with LuxI and GFP</b></p>
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<p>This part consists of the genetic switch described in BBa_K1718002 and the LuxI and GFP coding regions described in BBa_K1718004</p>
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{{CU_Boulder_footer}}

Latest revision as of 15:22, 18 September 2015


<!DOCTYPE html> Team:CU_Boulder - 2015.igem.org

Composite Parts



BBa_K1718001:

RFP under arabinose inducible pBad

This part has a Red Fluorescent Protein (RFP) downstream of the pBad promoter. Expression is stimulated by L-arabinose. We used this part to test the leakiness of this promoter.



BBa_K1718003:

RFP under the LuxPr promoter

In the presence of AHL, the LuxR transcription factor will bind AHL and activate the LuxPr promoter. RFP is downstream and will be expressed.



BBa_K1718004:

LuxI and GFP

LuxI leads to the production of AHL which, when in the presence of AHL, activates the LuxPr promoter. Downstream of the LuxI coding region is GFP, a reporter



BBa_K1718006:

Genetic switch for Bxb1 with LuxI and GFP

This part consists of the genetic switch described in BBa_K1718002 and the LuxI and GFP coding regions described in BBa_K1718004

Team:CU-Boulder - 2015.igem.org