Difference between revisions of "Team:China Tongji/Safety"

 
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                <li><img height="533" src="https://static.igem.org/mediawiki/2015/a/aa/China-Tongji-safety-figure1.jpg">
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                    <p class="imgsName">Figure1: We use ethanol to sterilize our gloves before the experiments.</p>
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                <li><img height="533" src="https://static.igem.org/mediawiki/2015/8/89/China-Tongji-safety-figure2.jpg">
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                    <p class="imgsName">Figure2: We prevent the C.elegans incubator from being directly exposed to the environment.</p>
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                <li><img height="533" src="https://static.igem.org/mediawiki/2015/3/3e/China-Tongji-safety-figure3.jpg">
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                    <p class="imgsName">Figure3:  We use UV light to sterilize the laminar bench everytime we finish our experiments.</p>
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                <li><img height="533" src="https://static.igem.org/mediawiki/2015/0/05/China-Tongji-safety-figure4.jpg">
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                    <p class="imgsName">Figure4: We did most of the experiments on C.elegans and E.colis in the  laminar flow bench.</p>
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                <li><img src="https://static.igem.org/mediawiki/2015/a/aa/China-Tongji-safety-figure1.jpg" alt="Figure1: We use ethanol to sterilize our gloves before the experiments."></li>
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                <li><img src="https://static.igem.org/mediawiki/2015/8/89/China-Tongji-safety-figure2.jpg" alt="Figure2: The C.elegans incubator，we keep them in that to prevent them from being directly exposed to the environment."></li> 
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                <li><img src="https://static.igem.org/mediawiki/2015/3/3e/China-Tongji-safety-figure3.jpg" alt="Figure3: The laminar bench we use,we use UV light to sterilize it everytime we finish our experiments."></li>
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                <li><img src="https://static.igem.org/mediawiki/2015/0/05/China-Tongji-safety-figure4.jpg" alt="Figure4: We did most of the experiments on C.elegans and E.colis in the  laminar flow bench."></li> 
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        <!-- 1. Safety -->
 
         <p class="titleOne" id="SafetySub">1. Safety</p>   
 
         <p class="titleOne" id="SafetySub">1. Safety</p>   
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         <p class="contentP">Safety is prior to all other stuffs especially in the lab.</p>
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        Cells sense the environment, process information, and make response to stimuli. To make cells work well in complex natural environments, lots of processes have to be preset to react to various signals. However, when well-characterized modules are combined to construct higher order systems, unpredictable behaviors often occur because of the interplay between modules. Another significant problem is that complex integrated systems composed of numerous parts may cause cell overload.</p>
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<p class="contentP">The Administrative office of Laboratory and Equipment in Tongji University is responsible for the safety of biology labs. Before we freshmen get into the lab and do experiments, we must take some courses offered by Administrative office of Laboratory and Equipment and have several tests to qualify our safety training on <a href="http://aqjy.tongji.edu.cn" target="_blank"><span class="linkWords">the Tongji University website</span></a>. </p>
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<p class="contentP">After taking the cources and passing the tests, we became familiar with some rules to secure the biosafety such as wearing the white coat and gloves is the necessity before doing the experiment, never bring the experiment materials out of the lab and never eat or drink inside the lab. And later all our members also sign up on a letter of commitment in which we promise to obey all the rules to secure the lab safety and the lab manager has the right to prevent us from continuing the experiments if we did something harmful to safety. </p>
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        <p class="imgName" align="center">Figure 1. Schematic demonstration of HIV</p>
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<p class="contentP">In our project, we use 2 kinds of organism, C.elegans and E.colis. E.coli is ranked in the organisms of Level 1 risk goup and C.elegans is icluded in <a href="https://2015.igem.org/Safety/WhiteList" target="_blank"><span class="linkWords">the iGEM White List</span></a> which means that none of the organisms we are working with will cause any threat to the environment.</p>
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<p class="contentP">Even though the E.coli and the C.elegans we work with are not harmful for the environment and health, we still assume that they may threaten the ecology, so we wear rubber gloves and lab coat when we do the experiments to keep it from direct touching with human body. We also do all the experiments with these living organism in the laminar flow bench to prevent those organism from getting into the outside air. After we do the experiments, we use NaclO solution to sterilizing waste such as the used agar plate and the used tips. What's more, everytime after we finish our work, we use UV light to sterilize the lab.</p>
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        We proposed an elegant method to design higher order systems. Instead of merely combining different functional modules, we constructed one integrated processing module with fewer parts by utilizing the common structures between modules. The circuit we designed is a rewirable one and the topological structure of the processing module can be altered to <span style="font-weight:bold;">adapt</span> to environmental change. The basic idea is to rewire the connections between parts and devices to <span style="font-weight:bold;">implement multiple functions</span> with the help of the site-specific recombination systems.</p>
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<p class="contentP">In order to secure 100 percetnt safe, we talked to the lab manager,Sanyun Liu, making sure that all of our experiments on E.coli are safe and also make sure the C.elegans we work with are properly disposed after our experiments.</p>
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<p class="contentP">To see some details about the safety of our parts, please look through <a href="https://2015.igem.org/Safety/Final_Safety_Form?team_id=1634" target="_blank"><span class="linkWords">our Safety Form</span></a>.</p>
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        Our design approach may lead to a revolutionary step towards <span style="font-weight:bold;">system integration</span> in synthetic biology. Potential fields of application include organism development, living therapeutics and environment improvement.</p>
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Latest revision as of 15:46, 18 September 2015

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Safety

  • 1. Safety


    • 1. Safety

    Safety is prior to all other stuffs especially in the lab.

    The Administrative office of Laboratory and Equipment in Tongji University is responsible for the safety of biology labs. Before we freshmen get into the lab and do experiments, we must take some courses offered by Administrative office of Laboratory and Equipment and have several tests to qualify our safety training on the Tongji University website.

    After taking the cources and passing the tests, we became familiar with some rules to secure the biosafety such as wearing the white coat and gloves is the necessity before doing the experiment, never bring the experiment materials out of the lab and never eat or drink inside the lab. And later all our members also sign up on a letter of commitment in which we promise to obey all the rules to secure the lab safety and the lab manager has the right to prevent us from continuing the experiments if we did something harmful to safety.

    In our project, we use 2 kinds of organism, C.elegans and E.colis. E.coli is ranked in the organisms of Level 1 risk goup and C.elegans is icluded in the iGEM White List which means that none of the organisms we are working with will cause any threat to the environment.

    Even though the E.coli and the C.elegans we work with are not harmful for the environment and health, we still assume that they may threaten the ecology, so we wear rubber gloves and lab coat when we do the experiments to keep it from direct touching with human body. We also do all the experiments with these living organism in the laminar flow bench to prevent those organism from getting into the outside air. After we do the experiments, we use NaclO solution to sterilizing waste such as the used agar plate and the used tips. What's more, everytime after we finish our work, we use UV light to sterilize the lab.

    In order to secure 100 percetnt safe, we talked to the lab manager,Sanyun Liu, making sure that all of our experiments on E.coli are safe and also make sure the C.elegans we work with are properly disposed after our experiments.

    To see some details about the safety of our parts, please look through our Safety Form.


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