Difference between revisions of "Team:elan vital korea/safety"
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<a name="myAnchor" id="myAnchor"></a><br><br><br>Safety First!</h5> <br> | <a name="myAnchor" id="myAnchor"></a><br><br><br>Safety First!</h5> <br> | ||
<P style="text-align:center;"> | <P style="text-align:center;"> | ||
− | <font color=" | + | |
+ | <font color="black"> | ||
Saving the world starts with saving ourselves. You cannot save the world unless you’re in good shape. We put safety on the top priority <br> | Saving the world starts with saving ourselves. You cannot save the world unless you’re in good shape. We put safety on the top priority <br> | ||
in any lab works. We have been informed accurately of the lab safety rules as well as bio-safety regulations at the<br> | in any lab works. We have been informed accurately of the lab safety rules as well as bio-safety regulations at the<br> | ||
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− | <h5 style="text-align:center;"> | + | <P style="text-align:center;"> |
− | + | <font color="black"> | |
+ | So, Let's begin by watching a video:<br /><br /> | ||
+ | |||
+ | <video controls> | ||
+ | <source src="https://static.igem.org/mediawiki/2015/d/dd/Sequence_01_1.mp4" type="video/mp4"> | ||
+ | </video> | ||
+ | </font></p><br /><br /> | ||
+ | |||
+ | <h5 style="text-align:center;clear:both;"> | ||
+ | |||
+ | <font color="black"> | ||
The rule of thumb is: | The rule of thumb is: | ||
</font> | </font> | ||
</h5> | </h5> | ||
− | <div class=" | + | <div class="innerauto"> |
− | + | ||
− | <div class=" | + | <font color="black"> |
+ | |||
+ | <div class="caption0"> | ||
<h5> | <h5> | ||
− | <font color=" | + | |
+ | <font color="black"> 1 </font> | ||
</h5> | </h5> | ||
<p> | <p> | ||
− | Start with reading Safety Questions for overview. | + | Start with reading Safety Questions <br> |
+ | for overview. | ||
</p> | </p> | ||
</div> | </div> | ||
− | <div class=" | + | <div class="caption0"> |
<h5> | <h5> | ||
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+ | <font color="black"> 2</font> | ||
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− | + | <P style="text-align:center;clear:both"> | |
− | <P style="text-align:center;"> | + | |
<font color="black"> | <font color="black"> | ||
We have conducted our experiments at Gachon University. Thus, we have always observed safety rules of the lab. We had Safety Education & Training Session <br> | We have conducted our experiments at Gachon University. Thus, we have always observed safety rules of the lab. We had Safety Education & Training Session <br> | ||
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</font> | </font> | ||
</p> | </p> | ||
− | + | <br> | |
<h5 style="text-align:center;"> | <h5 style="text-align:center;"> | ||
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<h5> | <h5> | ||
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</h5> | </h5> | ||
<p> | <p> | ||
− | + | Labs should be maintained in <br> | |
− | + | clean, organized and orderly manner. | |
</p> | </p> | ||
</div> | </div> | ||
− | <div class=" | + | <div class="caption4"> |
<h5> | <h5> | ||
<font color="black"> 2 </font> | <font color="black"> 2 </font> | ||
</h5> | </h5> | ||
<p> | <p> | ||
− | + | All participants including <br> | |
+ | students and instructors <br> | ||
+ | must understand and observe <br> | ||
+ | safety rules and guidelines. | ||
</p> | </p> | ||
</div> | </div> | ||
− | <div class=" | + | <div class="caption4"> |
<h5> | <h5> | ||
<font color="black"> 3 </font> | <font color="black"> 3 </font> | ||
</h5> | </h5> | ||
<p> | <p> | ||
− | + | Before the lab activities, lab safety education must be | |
− | + | taken in which contents of lab experiments, operation methods | |
+ | of lab equipment, rules and guidelines, and procedures in case | ||
+ | of accidents must be properly understood. | ||
</p> | </p> | ||
</div> | </div> | ||
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<h5> | <h5> | ||
<font color="black"> 4 </font> | <font color="black"> 4 </font> | ||
</h5> | </h5> | ||
<p> | <p> | ||
− | + | Everyone must follow instructions <br> | |
+ | of the responsible person in <br> | ||
+ | the lab and should not perform <br> | ||
+ | any activities which may cause <br> | ||
+ | any danger. | ||
</p> | </p> | ||
</div> | </div> | ||
− | <div class=" | + | <div class="caption5"> |
<h5> | <h5> | ||
<font color="black"> 5 </font> | <font color="black"> 5 </font> | ||
</h5> | </h5> | ||
<p> | <p> | ||
− | + | Everyone must understand emergency behavior | |
− | + | guidelines. Emergency includes fire, <br> | |
+ | emergency patient and | ||
+ | other dangerous situation. Everyone must know the location of | ||
+ | emergent phone calls, fire alarm, and power disconnecting switch, etc. | ||
</p> | </p> | ||
</div> | </div> | ||
− | <div class=" | + | <div class="caption5"> |
<h5> | <h5> | ||
<font color="black"> 6 </font> | <font color="black"> 6 </font> | ||
</h5> | </h5> | ||
<p> | <p> | ||
− | + | Everyone must understand how to use fire extinguisher and check the status of it. | |
− | + | ||
− | + | ||
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</p> | </p> | ||
</div> | </div> | ||
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</h5> | </h5> | ||
<p> | <p> | ||
− | + | Smoking, eating, drinking, chewing the gum are prohibited in the lab. Disposal of food and drink is also forbidden. | |
− | + | ||
− | + | ||
− | + | ||
− | the | + | |
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</p> | </p> | ||
</div> | </div> | ||
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</h5> | </h5> | ||
<p> | <p> | ||
− | + | All participants must be properly dressed in the lab with the safety Consideration. | |
− | + | ||
− | + | ||
</p> | </p> | ||
</div> | </div> | ||
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</h5> | </h5> | ||
<p> | <p> | ||
− | + | During the operation of the equipment and <br> | |
− | + | during the experiment, at least <br> | |
− | + | one person should attend and observe the situation. | |
− | + | ||
</p> | </p> | ||
</div> | </div> | ||
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<section id="maintext2" data-speed="10" data-type="background"> | <section id="maintext2" data-speed="10" data-type="background"> | ||
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<div class="inner3"> | <div class="inner3"> | ||
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− | <div class=" | + | <font color="black"> |
+ | <div class="caption40"> | ||
<h5> | <h5> | ||
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+ | <font color="black"> 10 </font> | ||
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+ | <font color="black"> 11 </font> | ||
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− | <font color=" | + | |
+ | <font color="black"> 13 </font> | ||
</h5> | </h5> | ||
<p> | <p> | ||
− | + | Flammable material must not be used near firearms, and must be kept far away from the electric socket, air conditioner and/or heaters. | |
</p> | </p> | ||
</div> | </div> | ||
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− | <font color=" | + | |
+ | <font color="black"> 14 </font> | ||
</h5> | </h5> | ||
<p> | <p> | ||
− | + | Heaters and other heating devices that are not related to experiments must not be used in the lab. | |
− | + | ||
</p> | </p> | ||
</div> | </div> | ||
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− | <font color=" | + | |
+ | <font color="black"> 15 </font> | ||
</h5> | </h5> | ||
<p> | <p> | ||
− | + | Places where dangerous materials are kept must be identified with labels including the name of the material and the level of danger. (Explosiveness, spontaneous combustion, flammability, toxicity, water reactivity, oxidation, contagiousness, radioactivity, corrosiveness and other dangerous material.) | |
− | and | + | |
− | + | ||
− | + | ||
</p> | </p> | ||
</div> | </div> | ||
− | <div class=" | + | <div class="caption32"> |
<h5> | <h5> | ||
− | <font color=" | + | |
+ | <font color="black"> 16 </font> | ||
</h5> | </h5> | ||
<p> | <p> | ||
− | + | Chemicals must be kept in isolation in consideration of flammability, corrosiveness, and other chemical features. | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
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</p> | </p> | ||
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<h5> | <h5> | ||
− | <font color=" | + | |
+ | <font color="black"> 17 </font> | ||
</h5> | </h5> | ||
<p> | <p> | ||
− | + | When conducting chemical experimentation, tasting and smelling must not be allowed. Pipette using mouth must not be allowed. | |
− | + | ||
− | + | ||
</p> | </p> | ||
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+ | <font color="black"> 18 </font> | ||
</h5> | </h5> | ||
<p> | <p> | ||
− | <font color=" | + | |
− | + | <font color="black"> | |
− | + | Inflammable chemicals must be purchased <br> | |
− | + | in small amount when necessary.</font> | |
− | + | ||
− | </font> | + | |
</p> | </p> | ||
</div> | </div> | ||
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+ | <font color="black"> 27 </font> | ||
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− | + | <h5 style="text-align:center;clear:both"> | |
+ | |||
+ | <font color="black"> | ||
Other significant Safety Considerations. | Other significant Safety Considerations. | ||
</font> | </font> | ||
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<h5 style="text-align:center;"> | <h5 style="text-align:center;"> | ||
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+ | <font color="black"> | ||
1. Fire Regulations. | 1. Fire Regulations. | ||
</font> | </font> | ||
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<P style="text-align:center;"> | <P style="text-align:center;"> | ||
− | <font color=" | + | |
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When fire breaks out, the following guidelines shall apply. | When fire breaks out, the following guidelines shall apply. | ||
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<h5> | <h5> | ||
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+ | <font color="black"> 1) </font> | ||
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<br> | <br> | ||
− | <h5 style="text-align:center;"> | + | <h5 style="text-align:center;clear:both"> |
− | + | <font color="black"> | |
CHEMICALS | CHEMICALS | ||
</font> | </font> | ||
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<P style="text-align:center;"> | <P style="text-align:center;"> | ||
− | <font color=" | + | |
+ | <font color="black"> | ||
On each chemical container, there is a label that specifies the potential danger of the substance for humans and/or the environment. <br> | On each chemical container, there is a label that specifies the potential danger of the substance for humans and/or the environment. <br> | ||
Chemicals should be handled cautiously with gloves, both for your safety and for decreasing the contamination risk.<br> | Chemicals should be handled cautiously with gloves, both for your safety and for decreasing the contamination risk.<br> | ||
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<br> | <br> | ||
<h5 style="text-align:center;"> | <h5 style="text-align:center;"> | ||
− | + | <font color="black"> | |
BIO- SAFETY AND DISPOSAL | BIO- SAFETY AND DISPOSAL | ||
</font> | </font> | ||
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<P style="text-align:center;"> | <P style="text-align:center;"> | ||
− | <font color=" | + | |
+ | <font color="black"> | ||
When you are working to generate new organisms by molecular methods, you are regulated in most countries <br> | When you are working to generate new organisms by molecular methods, you are regulated in most countries <br> | ||
according to international biosafety guidelines: | according to international biosafety guidelines: | ||
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<div class="inner"> | <div class="inner"> | ||
− | <font color=" | + | |
+ | <font color="black"> | ||
<div class="caption2"> | <div class="caption2"> | ||
<h5> | <h5> | ||
− | <font color=" | + | |
+ | <font color="black"> 1 </font> | ||
</h5> | </h5> | ||
<p> | <p> | ||
− | + | <a href="http://www.cdc.gov/biosafety/publications/bmbl5/bmbl5_sect_iv.pdf"> <font color="black"> CDC, Atlanta. Biosafety in Microbiological and Biomedical Laboratories.</font> </a><br> | |
− | <a | + | |
</p> | </p> | ||
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<h5> | <h5> | ||
− | <font color=" | + | |
+ | <font color="black"> 2</font> | ||
</h5> | </h5> | ||
<p> | <p> | ||
− | + | <a href="http://www.who.int/csr/resources/publications/ biosafety/Biosafety7.pdf"> <font color="black"> WHO, Geneva. Laboratory Biosafety Manual, 2004.</font></a><br> | |
− | <a | + | |
</p> | </p> | ||
</div> | </div> | ||
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− | <font color=" | + | |
+ | <font color="black"> 3 </font> | ||
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− | ECDC, Directive 2000/54/ec of the European parliament and of the Council of 18 September 2000 on the protection of workers from risks related to exposure to biological agents at work (seventh individual directive within the meaning of Article 16(1) of Directive 89/391/EEC. < | + | <a href="http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2000:262:0021:0045:EN:PDF"> <font color="black"> ECDC, Directive 2000/54/ec of the European parliament and of the Council of 18 September 2000 on the protection of workers from risks related to exposure to biological agents at work (seventh individual directive within the meaning of Article 16(1) of Directive 89/391/EEC.</font> </a> <br> |
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− | Institutional Bio-safety Committee, under the ministry of Health and Welfare, Korea Center for Disease Control & Prevention. < | + | <a href="http://biosafety.cdc.go.kr"> <font color="black"> Institutional Bio-safety Committee, under the ministry of Health and Welfare, Korea Center for Disease Control & Prevention. </font> </a> <br> |
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Latest revision as of 16:38, 18 September 2015
Safety First!
Saving the world starts with saving ourselves. You cannot save the world unless you’re in good shape. We put safety on the top priority
in any lab works. We have been informed accurately of the lab safety rules as well as bio-safety regulations at the
initial stage of the project in order to understand hazards and safety procedures. Also we received safety training from our instructor
before commencing our lab work. Even though our project is included Bio-Safety Level 1, we know very well that
there are still many risks that may be escalated into serious disaster. Furthermore, even when handling those risks that may not pose serious
threats to the experienced scientists, we as young learners should be extra careful.
So, Let's begin by watching a video:
The rule of thumb is:
We have conducted our experiments at Gachon University. Thus, we have always observed safety rules of the lab. We had Safety Education & Training Session
before starting our lab work. Also, when we conducted our experiments, Gachon University staff supervised our work.
Gachon University General Lab Safety Rule
Other significant Safety Considerations.
1. Fire Regulations.
When fire breaks out, the following guidelines shall apply.
Also it is very important to know the locations of the fire-fighting equipment, fire alarms, and evacuation routes closest to the lab.
A small fire can be extinguished quickly by smothering it in a fire blanket or by spraying it with a fire extinguisher.
Lab fires in biological labs are caused most commonly by the plating of cell cultures.
To prevent this,
CHEMICALS
On each chemical container, there is a label that specifies the potential danger of the substance for humans and/or the environment.
Chemicals should be handled cautiously with gloves, both for your safety and for decreasing the contamination risk.
Always wear a lab coat and shoes as additional protection. Read the signs on the chemical container and the Material Safety Data Sheet
(MSDS; available online) for further direction.
BIO- SAFETY AND DISPOSAL
When you are working to generate new organisms by molecular methods, you are regulated in most countries
according to international biosafety guidelines:
When working with microorganisms such as bacteria and viruses, there are four BioSafety Levels (BSL) numbered BSL1–4.
Biosafety level is the level of the biocontainment precautions required to isolate dangerous biological agents in an enclosed
The levels of containment range from the lowest biosafety level 1 to the highest at level 4, and Ministry of Health and Welfare and Korea Centers
for Disease Control and Prevention have specified these levels facility.
Biocontainment can be classified by the relative danger to the surrounding environment as biological
safety levels. As of 2006, there are four safety levels. These are called BSL1 through BSL4