Difference between revisions of "Team:China Tongji/Outreach"
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− | <p class="contentP">The higher the score, the more important people think the aspect is. They think “Follow the rules in lab” and “Mine safety training to the | + | <p class="contentP">The higher the score ranks, the more important people think the aspect is. They think “Follow the rules in lab” and “Mine safety training to the students”are the most important. These shows that people may know something about the rules in lab, but we still should supervise them to obey it. </p> |
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<h3>3.2.7 What do you think of transgenosis?</h3> | <h3>3.2.7 What do you think of transgenosis?</h3> |
Revision as of 16:44, 18 September 2015
![](https://static.igem.org/mediawiki/2015/9/92/China-Tongji-outreachBug.png)
Outreach
1. Human Practice
- 1.1 Three Teams Communication Meeting 1
- 1.2 Three Teams Communication Meeting 2
- 1.3 Popularize Scientific Knowledge about Synthetic Biology
- 1.4 Communication Meeting with SYSU-China
- 1.5 Exhibition at Shanghai Technology Museum
2. Collaboration
- 2.1 Collaboration with SJTU-BioX-Shanghai
- 2.2 Collaboration with SJTU-Software
3. Questionare
- 3.1 Introduction
- 3.2 Questionare
- 3.3 Analyses
- 3.4 Conclusion
1. Human Practice
This year, our team mainly focus on communicating with other iGEM teams and raising public awareness towards synthetic biology. Through our practice, we have learned more from each other and built sincere friendship among our team and other teams. Moreover, we promoted the public’s understanding of iGEM and our project.
1.1 Three Teams Communication Meeting 1 -- 2015.4.19
On April 19th, we went to Shanghai Jiaotong University to have a communication meeting .Three teams including SJTU-BioX-Shanghai, NJAU_China and Tongji have attended. In this meeting, we first introduced our project respectively. Then we raised some question and gave some advice on other teams projects. We not only considered these suggestions and perfected our projects but made friends with each other as well.
1.2 Three Teams Communication Meeting 2 -- 2015.6.8
On Jun 8th we had a meet-up with NYU Shanghai, SJTU and a behalf from ITB, at NYU Shanghai. This culture-mixed gathering covered not only those topics on our projects, but exchanging of our daily experiences as well. After this happy meeting, we had a general understanding about each other’s idea and set up a long-term union for mutual assistance.
1.3 Popularize Scientific Knowledge about Synthetic Biology -- 2015.6.22
On June 22nd,we had a class in Tongji primary school.
Firstly, we introduced iGEM and synthetic biology to them. Then, we briefly explained our project. We had adapted our project for a interesting story. So in this class, we showed them this story by PPT and our explanation. They were very interested about C.elegans and asked many questions about it.
After this class, these kids had more knowledges about synthetic biology and iGEM. We achieved our goal that popularizing scientific knowledge.
1.4 Communication Meeting with SYSU-China -- 2015.7.11
On July 11st, we communicated with the captain of SYSU-China. We exchanged some problems and difficulties that we met in our experimental process. This led us to make a summing up of the experience and lessons.
We showed our logo and gave some advice to each other. What’s more, we talked about the construction of our wikis.
![](https://static.igem.org/mediawiki/2015/8/8b/China-Tongji-humanPractice-20150711-1.jpg)
1.5 Exhibition at Shanghai Technology Museum -- 2015.8.8
On Aug 8th, with 6 other iGEM teams, we made an exhibition at Shanghai Technology Museum. We introduced our project and the iGEM competition to the people who visited the museum that day. More than 5000 people have seen our project, what’s more, we had a good communication with other teams about our projects!
Teams include: NYU-Shanghai, Tongji, FDU, SJTU, SJTU-software, XJTLU, ZJU.
2. Collaboration
2.1 Collaboration with SJTU-BioX-Shanghai
-- Help them start their project
![](https://static.igem.org/mediawiki/2015/0/04/China-Tongji-Outreach-figure-2-1.jpg)
SJTU-BioX-Shanghai
2.1.1 Offer them special bacteria
The SJTU-BioX-Shanghai wants to use the bacterial luciferase in the Vibrio fischeri, and Professor Zhifen Lin in College of Environmental Science and Engineering in Tongji University has done a lot of research on Vibrio fischeri .So we help them to communicate with Professor Lin to give the SJTU-BioX-Shanghai some Vibrio fischerifor their project. And we also learn the using method including the culturing method, the molecular cloning method, then we teach the SJTU-BioX-Shanghai how to work with the strain and extract the bacterial luciferase.
2.1.2 Give some advises for their project
Our project is focus on Optogenetics and channelrhodopsins. At the meantime, the SJTU-BioX-Shanghai starts their project on halorhodopsin. Since our projects are all basing on the transmembrane rhodopsin, so we communicate a lot about our projects. The transmembrane rhodopsin needs the retinaldehyde(ATR) as its cofactor. However, SJTU-BioX-Shanghai didn’t know how to make the solution of ATR and prepare it for the culture medium of their algae. So we taught them the relevant experimental method and the mixture system.
2.2 Collaboration with SJTU-Software
-- Help them test and improve their software
![](https://static.igem.org/mediawiki/2015/6/63/China-Tongji-Outreach-figure-2-2.png)
SJTU-Software
2.2.1 How we collaborate with each other
SJTU-Software built up a multi-level biobrick evaluation and visualization system。Many existing biobricks are not well documented, which make it challenging to use them. They present a web-based tool BASE (Biobrick Auxiliary Selection Explorer) to search, evaluate and visualize biobricks at part, devise and system levels. Biobrick parts are scored according to their current status, user comments and reviews, times of citation and etc. At device level, features, such as the order of parts and the co-existence frequency of two parts in a device, are added into the scoring system. At each level, the biobricks can be searched, evaluated and ranked according to their scores.
We provide them with our parts using in this year’s project including not only the parts we submit ( BBa_K1634002, BBa_K1634003, BBa_K1634004, BBa_K1634007, BBa_K1634008 ) but also some parts which we constructed and used in our experiments such as ppD95.75_Pmyo3-chETA-EYFP. Then they use their software to score our parts. Finally, we compared the parts' characterization in our wet experiment with their output, and offered them some feedbacks to help them improve their scoring system.
The analysis result they give us:
“This year, TJU used the optogenetics technology to control the simple movement of nematodes. In the pathognosticmotoneuron and muscles of nematodes,the opsin will be expressed, which is a type of photosensitive channel protein that will be activated at a specific wavelength of light. Then it will stimulate some neurons and ultimately achieve the purpose of nematode movement control.
However, during the evaluation, we found that some of their biobricks are so new that we nearly couldn't find them in our database which covered the data in the iGEM official website. The result showed that those biobricks were never synthesized before.
Beyond that, we also found the regulator BBa_K1634003 is almost 80% similar to BBa_K309026 which is made by a team from Canada. This result might be used to compare the function of their biobricks with the other team; and explored more useful information from the alike one. ”
2.2.2 Feedback
Through this cooperation, we help them find advantages and disadvantages of their software.
Advantage:
They have focused their software on scoring the parts but ignored there is another strong function of their software, which is helping users to search for a similar or relative parts more efficiently, thus helps a lot for comparing and improving their parts. SJTU-Software starts pay more attention on this function and improve it.
Disadvantage:
During the evaluation, they found that our biobricks are so new that they couldn't find them in our database which covered the data in the iGEM official website. The result showed that those biobricks were never synthesized before. Then they couldn’t score our parts. Since most of the parts submitted by iGEM teams are new, we suggest them expanding their data source to cover more parts which can make it a more functional scoring system.
3. Questionare
3.1 Introduction
In order to know people' attitudes to iGEM ,Synthetic biology and Biology safety, we made this survey. From the result we have received, we get many useful information.
There are altogether 317 person filled in our questionnaire. Most of them(95.9%) come from all over China such as Beijing, Shanghai, Guangdong, etc. Some of them(4.1%) come from other countries such as Congo-Brazzaville, Australia and so on.
3.2 Questionare
China_Tongji’s questionnaire about iGEM and synthetic biology.
3.2.1 How old are you?
option | 10-16 years old | 17-22 years old | 23-30 years old | More than 30 years old |
---|---|---|---|---|
317 | 4 | 155 | 39 | 119 |
Different age levels people have filled in this questionnaire, which demonstrates that our research is significative. Most of them are teenagers from 17-22 years old. It shows that teenagers are more interested to iGEM.
3.2.2 What's your major (or the major you'd like to study)?
option | Biology | Science (beside Biology) | Engineering | Liberal arts |
---|---|---|---|---|
317 | 58 | 70 | 126 | 63 |
People in different majors have filled in this questionnaire, which demonstrates that our research is significative. So we can unite other majors people to join in iGEM in the future. What’s more, we might set up an organization to help other students learn more knowledges about iGEM.
3.2.3 Do you know iGEM (International Genetically Engineered Machine Competition)
option | I had join in that! | I know it. | Only heard. | What's that? |
---|---|---|---|---|
317 | 11 | 19 | 108 | 179 |
It’s necessary to enhance people’knowledge about iGEM, because there are more than 50% people said that they never heard iGEM before! Only 3.47% people have joint it.
3.2.4 What do you think of Synthetic biology? (Multiple-choice)
Synthetic biology is an interdisciplinary branch of biology, combining disciplines such as biotechnology, evolutionary biology, molecular biology, systems biology, biophysics, computer engineering, and genetic engineering.
Option | Subtotal | proportion |
---|---|---|
I know it well. | 11 | 3.47% |
Know something about it. | 41 | 12.93% |
Only heard. | 134 | 42.27% |
I don't know this. | 99 | 31.23% |
I support that. | 143 | 45.11% |
I worry about that. | 44 | 13.88% |
I object to such thing! | 2 | 0.63% |
The total number | 317 |
option | I know it well. | Know something about it. | Only heard. | I don't know this. | I support that. | I worry about that. | I object to such thing! |
---|---|---|---|---|---|---|---|
317 | 11 | 41 | 134 | 99 | 143 | 44 | 2 |
This question is a multiple-choice question. Most people just hear about Synthetic biology or even don’t know it .However, most people have positive attitude to Synthetic biology and support it. So we can do more propagandize to change the mind of people who have opposed attitude to Synthetic biology.
3.2.5 For Synthetic biology, safety and science, which is the most important?
option | Safety is more important. | They are equal important. | Who cares about safety? |
---|---|---|---|
317 | 201 | 112 | 4 |
Most people think that safety is more important than science. I appreciate their choice because I think that the development of science is also need safety. Meanwhile, we won’t give up studying biology. Safety and science must advance side by side.
3.2.6 Rank the options below by the safety level that you think.
Option | Average score |
---|---|
Follow the rules in lab. | 5.62 |
Mine safety training to the students. | 4.66 |
Keep experiment materials only in lab. | 4.31 |
Biology rubbish grading. | 4.28 |
Specialty company deal the Biology rubbish. | 3.61 |
Insist aseptic operation. | 3.39 |
Do propaganda and education work about Biology safety. | 2.42 |
incineration or buryed. | 0.8 |
Others. | 0.36 |
The higher the score ranks, the more important people think the aspect is. They think “Follow the rules in lab” and “Mine safety training to the students”are the most important. These shows that people may know something about the rules in lab, but we still should supervise them to obey it.
3.2.7 What do you think of transgenosis?
option | It can makes our life be better. | They may create a devil! | There are both advantages and disadvantages. |
---|---|---|---|
317 | 136 | 27 | 154 |
Only a few people think transgenosis is vicious thing. Most people think it has both advantages and disadvantages, we should adopt its good points and avoid its shortcomings.
3.2.8 Do you think Synthetic biology is useful?
option | Sure! It have broad prospects. | It is hard to apply. | I don't think they can success. |
---|---|---|---|
317 | 217 | 90 | 10 |
Most people think Synthetic biology is of great significance. We can create many interesting organism that never exist before. We may apply these research achievement in production and scientific research.
3.3 Analyses
Chart 3-1: Relationship of the major and the knowledge about iGEM
![800,380 Chart 3-1: Relationship of the major and the knowledge about iGEM](https://static.igem.org/mediawiki/2015/7/78/China-Tongji-Outreach-Questionare-chart1.jpg)
From thischart, we can see that people in Biology, Science and Engineering know iGEM better.
Chart 3-2: the opinions people hold about synthetic biology of different ages
![800,380 Chart 3-2: the opinions people hold about synthetic biology of different ages](https://static.igem.org/mediawiki/2015/6/65/China-Tongji-Outreach-Questionare-chart2.jpg)
We have found in this chart that the young men have more confidence about Synthetic biology.
3.4 Conclusion
From these results, we can come to the conclusion that the popularity of iGEM is barely satisfactory. So it’s necessary for us to introduce iGEM and Synthetic biology to more people.And that is what we have tried to do.
The good news is that many people have high awareness of Biology safety.And they think it's possible to balance development and safety.Thus we will pay more attention to safety in our experiment.
Particularly worth mentioning is the moral support that many people give to us .Most of them have positive attitudes to Synthetic biology,which encourages us a lot.
![](https://static.igem.org/mediawiki/2015/9/96/China-Tongji-Team-sponser-GENEWIZ.jpg)
![](https://static.igem.org/mediawiki/2015/f/f5/China-Tongji-Team-sponser-THORLABS.jpg)
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