Difference between revisions of "Team:Bielefeld-CeBiTec/Primers"

 
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Line 11: Line 11:
 
     });
 
     });
 
});
 
});
 +
$("#notebook").addClass("navbar-active");
 
</script>
 
</script>
  
Line 20: Line 21:
 
<div class="jumbotron-text">
 
<div class="jumbotron-text">
 
<h1 style="margin-bottom: 0px">Primers</h1>
 
<h1 style="margin-bottom: 0px">Primers</h1>
−
<p>...</p>
+
<p>Small in size, but great in performance!</p>
 
</div>
 
</div>
 
</div>
 
</div>
  
−
  <div class="row">
+
 
−
   <div class="col-md-2">
+
   <div class="col-md-8 col-md-offset-1">
−
</div>
+
−
  <div class="col-md-8">
+
  
 
     <table class="table table-hover primers">
 
     <table class="table table-hover primers">
Line 41: Line 40:
 
                 <td colspan="2">Standard primers</td>
 
                 <td colspan="2">Standard primers</td>
 
             </tr>
 
             </tr>
 +
<tr id="mRFP1_rev">
 +
<td>mRFP1_rev</td>
 +
<td>GGACGACCTTCACCTTCACCTTCACC</td>
 +
</tr>
 
           <tr id="SB-prep-2Ea">
 
           <tr id="SB-prep-2Ea">
 
                 <td>SB-prep-2Ea</td>
 
                 <td>SB-prep-2Ea</td>
Line 57: Line 60:
 
                 <td>GTATTACCGCCTTTGAGT</td>
 
                 <td>GTATTACCGCCTTTGAGT</td>
 
             </tr>
 
             </tr>
−
 
  
 
             <tr class="primers-subheading">
 
             <tr class="primers-subheading">
Line 64: Line 66:
 
             <tr id="Ars_1_rev">
 
             <tr id="Ars_1_rev">
 
                 <td>Ars_1_rev</td>
 
                 <td>Ars_1_rev</td>
−
                 <td>tatgacttaacgaatgtgtaagtgcagaggCGGATAAAACACATCTGAAAATTC</td>
+
                 <td>TATGACTTAACGAATGTGTAAGTGCAGAGGCGGATAAAACACATCTGAAAATTC</td>
 
             </tr>
 
             </tr>
 
             <tr id="Ars_2_fwd">
 
             <tr id="Ars_2_fwd">
 
                 <td>Ars_2_fwd</td>
 
                 <td>Ars_2_fwd</td>
−
                 <td>cctctgcacttacacattcgttaagtcataGGTACTAGTAGCGGCC</td>
+
                 <td>CCTCTGCACTTACACATTCGTTAAGTCATAGGTACTAGTAGCGGCC</td>
 
             </tr>
 
             </tr>
 
           <tr id="Ars_3_fwd">
 
           <tr id="Ars_3_fwd">
 
                 <td>Ars_3_fwd</td>
 
                 <td>Ars_3_fwd</td>
−
                 <td>cattcgttaagtcatatatgtttttgacttGGTACTAGTAGCGGCC</td>
+
                 <td>CATTCGTTAAGTCATATATGTTTTTGACTTGGTACTAGTAGCGGCC</td>
 
             </tr>
 
             </tr>
 
           <tr id="Ars_3_rev">
 
           <tr id="Ars_3_rev">
 
                 <td>Ars_3_rev</td>
 
                 <td>Ars_3_rev</td>
−
                 <td>tcaaaaacatatatgacttaacgaatgtgtCGGATAAAACACATCTGAAAATTC</td>
+
                 <td>TCAAAAACATATATGACTTAACGAATGTGTCGGATAAAACACATCTGAAAATTC</td>
 
             </tr>
 
             </tr>
 
           <tr id="arsRhis_arsR_fwd">
 
           <tr id="arsRhis_arsR_fwd">
 
                 <td>arsRhis_arsR_fwd</td>
 
                 <td>arsRhis_arsR_fwd</td>
−
                 <td>tatagggaaagaggagaaatactagagATGTCATTTCTGTTACCCATC</td>
+
                 <td>TATAGGGAAAGAGGAGAAATACTAGAGATGTCATTTCTGTTACCCATC</td>
 
             </tr>
 
             </tr>
 
           <tr id="arsRhis_arsR2_r">
 
           <tr id="arsRhis_arsR2_r">
 
                 <td>arsRhis_arsR2_r</td>
 
                 <td>arsRhis_arsR2_r</td>
−
                 <td>ctagtattagtggtgatggtgatgatgACTGCAAATGTTCTTACTG</td>
+
                 <td>CTAGTATTAGTGGTGATGGTGATGATGACTGCAAATGTTCTTACTG</td>
 
             </tr>
 
             </tr>
 
           <tr id="arsRhis_Trp_fwd">
 
           <tr id="arsRhis_Trp_fwd">
Line 96: Line 98:
 
           <tr id="UTR_arsO_rev">
 
           <tr id="UTR_arsO_rev">
 
                 <td>UTR_arsO_rev</td>
 
                 <td>UTR_arsO_rev</td>
−
                 <td>ccttcttttttttttttaaagttaaacaaaattatTAAGTCAAAAACATATATGACTTAACG</td>
+
                 <td>CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTAAGTCAAAAACATATATGACTTAACG</td>
−
            </tr>
+
          <tr id="UTR_arsR_fwd">
−
           <tr id="UTR_noT7_rev">
+
                <td>UTR_arsR_fwd</td>
−
                 <td>UTR_noT7_rev</td>
+
                <td>GTTTAACTTTAAAAAAAAAAAAGAAGGAGATATACATATGTCATTTCTGTTACCCATCC</td>
−
                 <td>ccttcttttttttttttaaagttaaacaaaattattCTCTAGAAGCGGCCGCGA</td>
+
           <tr id="UTR_arsR_rev">
−
            </tr>
+
                 <td>UTR_arsR_rev</td>
 +
                 <td>CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTCCCTATAGTGAGTCGTATTACTC</td>
 +
       
 +
       
 +
<tr id="UTR_arsR_fwd">
 +
<td>UTR_arsR_fwd</td>
 +
<td>GTTTAACTTTAAAAAAAAAAAAGAAGGAGATATACATATGTCATTTCTGTTACCCATCC</td>
 +
</tr>
 +
 
 +
<tr id="UTR_arsR_rev">
 +
<td>UTR_arsR_rev</td>
 +
<td>CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTCCCTATAGTGAGTCGTATTACTC</td>
  
 
<tr class="primers-subheading">
 
<tr class="primers-subheading">
Line 128: Line 141:
 
  <tr id="pZ9_pT7_rne_fwd">
 
  <tr id="pZ9_pT7_rne_fwd">
 
                 <td>pZ9_pT7_rne_fwd</td>
 
                 <td>pZ9_pT7_rne_fwd</td>
−
                 <td>gtccgcaacctgttgagtaaCTGCAGGAAGGGGATCCG</td>
+
                 <td>GTCCGCAACCTGTTGAGTAACTGCAGGAAGGGGATCCG</td>
 
             </tr>
 
             </tr>
 
<tr id="pZ9_pT7_rne_rev ">
 
<tr id="pZ9_pT7_rne_rev ">
 
                 <td>pZ9_pT7_rne_rev</td>
 
                 <td>pZ9_pT7_rne_rev</td>
−
                 <td>tagtatttctcctctttctctagtatctcCCTATAGTGAGTCGTATTAC</td>
+
                 <td>TAGTATTTCTCCTCTTTCTCTAGTATCTCCCTATAGTGAGTCGTATTAC</td>
 
             </tr>
 
             </tr>
  
Line 205: Line 218:
 
                 <td>TTATTATTTATACAGCTCATCCATACC</td>
 
                 <td>TTATTATTTATACAGCTCATCCATACC</td>
 
             </tr>
 
             </tr>
 +
  </tr>
 +
<tr id="UTR_noT7_rev">
 +
<td>UTR_noT7_rev</td>
 +
<td>CCTTCTTTTTTTTTTTTAAAGTTAAAGTTAAACAAAATTATTCTCTAGAAGCGGCCGCGA</td>
 +
</tr>
 +
 
<tr id="UTR_fwd">
 
<tr id="UTR_fwd">
 
                 <td>UTR_fwd</td>
 
                 <td>UTR_fwd</td>
Line 240: Line 259:
 
           <tr id="pSB1C3_BlcR_fwd">
 
           <tr id="pSB1C3_BlcR_fwd">
 
                 <td>pSB1C3_BlcR_fwd</td>
 
                 <td>pSB1C3_BlcR_fwd</td>
−
                 <td>cttgggtaccgatcgcagaaagactagTACTAGTAGCGGCCGCTGCAG</td>
+
                 <td>CTTGGGTACCGATCGCAGAAAGACTAGTACTAGTAGCGGCCGCTGCAG</td>
 
             </tr>
 
             </tr>
  
 
<tr id="pSB_BlcRfwd_new">
 
<tr id="pSB_BlcRfwd_new">
−
<td>psB_BlcRfwd_new"</td>
+
<td>psB_BlcRfwd_new</td>
 
<td>CTTGGGTACCGATCGCAGAAAGACTAATACTAGTAGCGGCCGCTGCAG</td>
 
<td>CTTGGGTACCGATCGCAGAAAGACTAATACTAGTAGCGGCCGCTGCAG</td>
 
</tr>
 
</tr>
Line 250: Line 269:
 
           <tr id="pSB1C3_BlcR_rev">
 
           <tr id="pSB1C3_BlcR_rev">
 
                 <td>pSB1C3_BlcR_rev</td>
 
                 <td>pSB1C3_BlcR_rev</td>
−
                 <td>ttgacacacttggcccctttggcccatctagtaTTTCTCCTCTTTCTCTAGTAGCTAGCACAATAC</td>
+
                 <td>TTGACACACTTGGCCCCTTTGGCCCATCTAGTATTTCTCCTCTTTCTCTAGTAGCTAGCACAATAC</td>
 
             </tr>
 
             </tr>
  
Line 263: Line 282:
  
 
<tr id="bclR-rv">
 
<tr id="bclR-rv">
−
<td>bclR-rv"</td>
+
<td>bclR-rv</td>
 
<td>GTCTTTCTGCGATCGGTACCC</td>
 
<td>GTCTTTCTGCGATCGGTACCC</td>
 
</tr>
 
</tr>
Line 284: Line 303:
 
           <tr id="K516030proB_rev">
 
           <tr id="K516030proB_rev">
 
                 <td>K516030proB_rev</td>
 
                 <td>K516030proB_rev</td>
−
                 <td>gggccaagtgtgtcaaggaaggtgcatCTCTAGAAGCGGCCGCGAATTCC</td>
+
                 <td>GGGCCAAGTGTGTCAAGGAAGGTGCATCTCTAGAAGCGGCCGCGAATTCC</td>
 
             </tr>
 
             </tr>
  
 
           <tr id="K516030proB_fwd">
 
           <tr id="K516030proB_fwd">
 
                 <td>K516030proB_fwd</td>
 
                 <td>K516030proB_fwd</td>
−
                 <td>tgaactctaatgcgggagaggtcgATGGCTTCCTCCGAAGACGTTAT</td>
+
                 <td>TGAACTCTAATGCGGGAGAGGTCGATGGCTTCCTCCGAAGACGTTAT</td>
 
             </tr>
 
             </tr>
  
Line 298: Line 317:
  
 
<tr id="BlcB_mut_rev">
 
<tr id="BlcB_mut_rev">
−
<td>BlcB_mut_rev"</td>
+
<td>BlcB_mut_rev</td>
 
<td>AGGGAACGGTGCGGAACTCGAAAGG</td>
 
<td>AGGGAACGGTGCGGAACTCGAAAGG</td>
 
</tr>
 
</tr>
Line 318: Line 337:
  
 
<tr id="K60_BlcPshort_f">
 
<tr id="K60_BlcPshort_f">
−
<td>K60_BlcPshort_f"</td>
+
<td>K60_BlcPshort_f</td>
 
<td>GCTAGCTACTAGAGCCATAGTTCACTCTAATGATTC</td>
 
<td>GCTAGCTACTAGAGCCATAGTTCACTCTAATGATTC</td>
 
</tr>
 
</tr>
Line 331: Line 350:
 
<td>AAGAAGGCTCCCGAATTTTACGCGTAATACTAGTAGCGGCCGCTGCAG</td>
 
<td>AAGAAGGCTCCCGAATTTTACGCGTAATACTAGTAGCGGCCGCTGCAG</td>
 
</tr>
 
</tr>
 +
 +
<tr id="Pblc_UTR_fwd">
 +
<td>Pblc_UTR_fwd</td>
 +
<td>TCAAGTTCAATTCGTTGCACTCTAATGAATAATTTTGTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATC</td>
 +
</tr>
 +
 +
<tr id="Pblc_UTR_rev">
 +
<td>Pblc_UTR_rev</td>
 +
<td>ATTGAACTTGAATCATTAGAGTGAACTCCCTATAGTGAGTCGTATTACTCTAG</td>
 +
</tr>
 +
 +
<tr id="BlcR-His_fwd">
 +
<td>BlcR-His_fwd</td>
 +
<td>CGTACTACCATCACCATCACCATCACGGCGCCATGGGCCAAAGGGGCC</td>
 +
</tr>
 +
 +
<tr id="BlcR-His_rev">
 +
<td>BlcR-His_rev</td>
 +
<td>CCGTGATGGTGATGGTGATGGTAGTACGACATCTAGTATTTCTCCTCTTTCTCTAGTA</td>
 +
</tr>
 +
 +
<tr id="gadR-qRT-fw">
 +
<td>gadR-qRT-fw</td>
 +
<td>TCAATCCCCTGTAACGG</td>
 +
</tr>
 +
 +
<tr id="gadR-qRT-rev">
 +
<td>gadR-qRT-rev</td>
 +
<td>CCAGGCGCACCGAACAAGAC</td>
 +
</tr>
 +
 +
<tr id="gadT-qRT-fw">
 +
<td>gadT-qRT-fw</td>
 +
<td>ATGAGTCAAACAACAGCAAGCATC</td>
 +
</tr>
 +
 +
<tr id="gadT-qRT-rev">
 +
<td>gadT-qRT-rev</td>
 +
<td>CTCAACCACCTTCGGATG</td>
 +
</tr>
 +
 +
<tr id="BlcR_oligorev">
 +
                <td>oligo_BlcR_51_rev</td>
 +
                <td>CCCGCATTAGAGTTCAACTAATTGAACTTGAATCATTAGAGTGAACTA
 +
TGG</td>
 +
            </tr>
 +
<tr id="BlcR_oligofwd">
 +
                <td>oligo_BlcR_51_fwd
 +
_biot</td>
 +
                <td>Biotin_CCATAGTTCACTCTAATGATTCAAGTTCAATTAGTTGAACTCT
 +
AATGCGGG</td>
 +
            </tr>
 +
<tr id="BlcR_oligorev_cy3">
 +
                <td>oligo_BlcR_51_rev_Cy3</td>
 +
                <td>Cy3_CCCGCATTAGAGTTCAACTAATTGAACTTGAATCATTAGAGTGAACTA
 +
TGG_Cy3</td>
 +
            </tr>
 +
<tr id="BlcR_oligofwd_cy3">
 +
                <td>oligo_BlcR_51_fwd
 +
_Cy3</td>
 +
                <td>Cy3_CCATAGTTCACTCTAATGATTCAAGTTCAATTAGTTGAACTCT
 +
AATGCGGG_Cy3</td>
 +
            </tr>
  
  
Line 339: Line 421:
 
           <tr id="GABA_B_pSB1C3_f">
 
           <tr id="GABA_B_pSB1C3_f">
 
                 <td>GABA_B_pSB1C3_f</td>
 
                 <td>GABA_B_pSB1C3_f</td>
−
                 <td>ataagaaaataccgacagggggatattcacTACTAGTAGCGGCCGCTGC</td>
+
                 <td>ATAAGAAAATACCGACAGGGGGATATTCACTACTAGTAGCGGCCGCTGC</td>
 
             </tr>
 
             </tr>
 
           <tr id="GABA_B_pSB1C3_r">
 
           <tr id="GABA_B_pSB1C3_r">
 
                 <td>GABA_B_pSB1C3_r</td>
 
                 <td>GABA_B_pSB1C3_r</td>
−
                 <td>cataaaaaaatcccagtcacaggtgattgaCTCTAGAAGCGGCCGCGA</td>
+
                 <td>CATAAAAAAATCCCAGTCACAGGTGATTGACTCTAGAAGCGGCCGCGA</td>
 
             </tr>
 
             </tr>
 
           <tr id="GABA_R_pSB1C3_f">
 
           <tr id="GABA_R_pSB1C3_f">
 
                 <td>GABA_R_pSB1C3_f</td>
 
                 <td>GABA_R_pSB1C3_f</td>
−
                 <td>tagttttcaaccctttcagcggaggagccatcgTACTAGTAGCGGCCGCTGC</td>
+
                 <td>TAGTTTTCAACCCTTTCAGCGGAGGAGCCATCGTACTAGTAGCGGCCGCTGC</td>
 
             </tr>
 
             </tr>
 
           <tr id="GABA_R_pSB1C3_r">
 
           <tr id="GABA_R_pSB1C3_r">
 
                 <td>GABA_R_pSB1C3_r</td>
 
                 <td>GABA_R_pSB1C3_r</td>
−
                 <td>ctgtggattaacacaccacctactttttaaCTCTAGAAGCGGCCGCGA</td>
+
                 <td>CTGTGGATTAACACACCACCTACTTTTTAACTCTAGAAGCGGCCGCGA</td>
 
             </tr>
 
             </tr>
 
           <tr id="Trp_sy_pSB1C3_f">
 
           <tr id="Trp_sy_pSB1C3_f">
 
                 <td>Trp_sy_pSB1C3_f</td>
 
                 <td>Trp_sy_pSB1C3_f</td>
−
                 <td>aaagaagttcatcatcaccatcaccactaaTACTAGTAGCGGCCGCTGC</td>
+
                 <td>AAAGAAGTTCATCATCACCATCACCACTAATACTAGTAGCGGCCGCTGC</td>
 
             </tr>
 
             </tr>
 
           <tr id="Trp_sy_pSB1C3_r">
 
           <tr id="Trp_sy_pSB1C3_r">
 
                 <td>Trp_sy_pSB1C3_r</td>
 
                 <td>Trp_sy_pSB1C3_r</td>
−
                 <td>tctcctctttccctatagtgagtcgtattaCTCTAGAAGCGGCCGCGA</td>
+
                 <td>TCTCCTCTTTCCCTATAGTGAGTCGTATTACTCTAGAAGCGGCCGCGA</td>
 
             </tr>
 
             </tr>
 +
     
 +
          <tr class="primers-subheading">
 +
                <td colspan="2">Heavy metals</td>
 +
            </tr>
 +
<tr id="cueR_rev">
 +
<td>cueR_rev</td>
 +
<td>TCACCCTGCCCGATGATGAC</td>
 +
</tr>
 +
 +
<tr id="cueR_fwd">
 +
<td>cueR_fwd</td>
 +
<td>AGTCCTAGGTATTGTGCTAGCTACTAGAGAAAGAGGAGAAAATGAACATCAGCGATGTAGC</td>
 +
</tr>
 +
 +
 +
<tr id="rcnR_PstI_fwd">
 +
<td>rnR_PstI_fwd</td>
 +
<td>CGAATGCGCAGTTTTAC</td>
 +
</tr>
 +
 +
<tr id="rcnR_PstI_rev">
 +
<td>rnR_PstI_rev</td>
 +
<td>GTAAAACTGCGGCGCATTCG</td>
 +
</tr>
 +
 +
<tr id="pSB1C3_copAP_fwd">
 +
<td>pSB1C3_copAP_fwd</td>
 +
<td>TTGACCTTCCCCTTGCTGGAAGGTTTAACCTTTATCACAATTAAAGAGGAGAAATACTAG</td>
 +
</tr>
 +
 +
<tr id="pSB1C3_copAP_rev">
 +
<td>pSB1C3_copAP_rev</td>
 +
<td>TAAACCTTCCAGCAAGGGGAAGGTCAAAGAAATTAATAAACTCTAGAAGCGGCCGCGAAT</td>
 +
</tr>
 +
 +
<tr id="pSB1C3_copA_rev">
 +
<td>pSB1C3_copA_rev</td>
 +
<td>GGTCAAGAAATTAATAAACCAGGCGGGTCTCTAGAAGCGGCCGCGAAT</td>
 +
</tr>
 +
 +
<tr id="Chrom2_pSB1C3_fwd">
 +
<td>Chrom2_pSB1C3_fwd</td>
 +
<td>AGCTTTGAAAAAGGGACCCTCACCGTATGATACTAGTAGCGGCCGCTGC</td>
 +
</tr>
 +
 +
<tr id="Chrom2_pSB1C3_rev">
 +
<td>Chrom2_pSB1C3_rev</td>
 +
<td>AATAAAAGCAAATGGCAATAGGAATAAGCAATCCTCTAGAAGCGGCCGCGA</td>
 +
</tr>
 +
 +
 +
<tr id="pSB1C3_cueR_pre">
 +
<td>pSB1C3_cueR_pre</td>
 +
<td>GGCTGCTGTCATCATCGGGCAGGGTGACTCTAGAAGCGGCCGCGAAT</td>
 +
</tr>
 +
 +
<tr id="pSB1C3_cueR_suf">
 +
<td>pSB1C3_cueR_suf</td>
 +
<td>CTAGTAGCTAGCACAATACCTAGGACTGAGCTAGCTGTCAATACTAGTAGCGGCCGCTGCA</td>
 +
</tr>
 +
 +
<tr id="pSB1C3_cueR_rev">
 +
<td>pSB1C3_cueR_rev</td>
 +
<td>GGTTGTTGTCATCATCGCGCAGGGTAACTCTAGAAGCGGCCGCGAAT</td>
 +
</tr>
 +
 +
<tr id="pSB1C3_rcnA_fwd">
 +
<td>pSB1C3_rcnA_fwd</td>
 +
<td>TAGATTAATAGTGCTATGATTTTTTATTGTTAACCAGGTGTTGCCATGTCTCATCTCTAGAAGCGGCCGCGAAT</td>
 +
</tr>
 +
 +
<tr id="pSB1C3_rcnA_rev">
 +
<td>pSB1C3_rcnA_rev</td>
 +
<td>TGAAAAATCATAGCACTATTAATCTAGGGGGGTAGTATCAGGTACTGGGGGGGAGTAATTAAAGAGGAGAAATACTA</td>
 +
</tr>
 +
 +
<tr id="pSB1C3_rcnR_rev">
 +
<td>pSB1C3_rcnR_rev</td>
 +
<td>AGTCCTAGGTATTGTGCTAGCTACTAGAGAAAGAGGAGAAAATGTCTCATACAATCCGTGATA</td>
 +
</tr>
 +
 +
<tr id="pSB1C3_rcnR_fwd">
 +
<td>pSB1C3_rcnR_fwd</td>
 +
<td>CGCGGCCGCTTCTAGAGTTACTTGATATATGAATCCAGC</td>
 +
</tr>
 +
 +
<tr id="pSB1C3_rcnR_f">
 +
<td>pSB1C3_rcnR_f</td>
 +
<td>CTAGTAGCTAGCACAATACCTAGGACTGAGCTAGCTGTAAATACTAGTAGCGGCCGCTGCAGTCC</td>
 +
</tr>
 +
 +
<tr id="pSB1C3_rcnR_rev">
 +
<td>pSB1C3_rcnR_rev</td>
 +
<td>GAAAGTCCTTGATTCGTACATCAAATGACTCTAGAAGCGGCCGCGAAT</td>
 +
</tr>
 +
 +
<tr id="pSB1C3_merR_suf">
 +
<td>pSB1C3_merR_suf</td>
 +
<td>CTAGCACAATACCTAGGACTGAGCTAGCTGTCAATAGTAGTAGCGGCCGCTGCAG</td>
 +
</tr>
 +
 +
<tr id="pSB1C3_merR_pre">
 +
<td>pSB1C3_merR_pre</td>
 +
<td>GGCCTGGCAAGGTCAGCTAGTCCTTAGCTCTAGAAGCGGCCGCGAAT</td>
 +
</tr>
 +
 +
 +
<tr id="rcnA_UTR_fwd">
 +
<td>rcnA_UTR_fwd</td>
 +
<td>CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTTTTCTCCTCTTTTACTCCCCCC</td>
 +
</tr>
 +
 +
<tr id="UTR_mod_fwd">
 +
<td>UTR_mod_fwd</td>
 +
<td>GTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATCTCTGCGTAAAGGCGAAGAGCTGTTCAC</td>
 +
</tr>
 +
 +
<tr id="MerR_fwd">
 +
<td>MerR_fwd</td>
 +
<td>TAGCTCAGTCCTAGGTATTGTGCTAGCAAAGAGGAGAAATACTAGATGG</td>
 +
</tr>
 +
 +
<tr id="merR_rev">
 +
<td>merR_rev</td>
 +
<td>CTAAGGCATAGCTGATCTTG</td>
 +
</tr>
 +
 +
<tr id="copAP_UTR_rev">
 +
<td>copAP_UTR_rev</td>
 +
<td>CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTCTAGTACTTTAAGACAGTTTTG</td>
 +
</tr>
 +
 +
<tr id="CrProm_UTR_rev">
 +
<td>CrProm_UTR_rev</td>
 +
<td>CTTTTTTTTTTTTAAAGTTAAACAAAATTATTGTCGTAGATGTTACTACAATAATGAG</td>
 +
</tr>
 +
 +
<tr id="pbrA_UTR_rev">
 +
<td>pbrA_UTR_rev</td>
 +
<td>CTCCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTCTAGTATTTCTCCTCTTTTTCTCGCGTTGCC</td>
 +
</tr>
 +
 +
<tr id="pSB1C3_merT_rev">
 +
<td>pSB1C3_merT_rev</td>
 +
<td>GGATAGCGTAACCTTACTTCCGTACTCATGTACGGAGTCAAGCGATATGGAACTCTAGAAGCGGCCGCGAATTC</td>
 +
</tr>
 +
 +
<tr id="merT_omRFP_fwd">
 +
<td>merT_omRFP_fwd</td>
 +
<td>CTCCGTACATGAGTACGGAAGTAAGGTTACGCTATCCAATCCTACTAGATTAAAGAGGAGAAATAC</td>
 +
</tr>
 +
 +
<tr id="1C3_pre_merT_rev">
 +
<td>1C3_pre_merT_rev</td>
 +
<td>CTTACTTCCGTACTCATGTACGGAGTCAAGCGATATGGAACTCTAGAAGCGGCCGCGAATTC</td>
 +
</tr>
 +
 +
 +
<tr id="UTR_merT_fwd">
 +
<td>UTR_merT_fwd</td>
 +
<td>GAGTACGGAAGTAAGGTTACGCTATCCAATCCTACGACAATAATTTTGTTTAACTTTAAAAAAAAAAAAG</td>
 +
</tr>
 +
 +
<tr id="pSB1C3_kPrm_fwd">
 +
<td>pSB1C3_kPrm_fwd</td>
 +
<td>CTAGGACTGAGCTAGCTGTCAATACTAGTAGCGGCCGCTGCA</td>
 +
</tr>
 +
 +
<tr id="pSB1C3_kPrm_r">
 +
<td>pSB1C3_kPrm_r</td>
 +
<td>GCACCACCGCGCACCCGTCCGATTAACTCTAGAAGCGGCCGCGAAT</td>
 +
</tr>
 +
 +
<tr id="pSB1C3-pbrR-fwd">
 +
<td>pSB1C3-pbrR-fwd</td>
 +
<td>CCACCGCGCACCCGTCCGATTAACTCTAGAAGGGCCGCGAAT</td>
 +
</tr>
 +
 +
<tr id="pSB1C3-pbrR-f">
 +
<td>pSB1C3-pbrR-f</td>
 +
<td>CAATACCTAGGACTGAGCTAGCTGTCAATACTAGTAGCGGCCGCTGCA</td>
 +
</tr>
 +
 +
<tr id="pSB1C3-pbrA_rev">
 +
<td>pSB1C3-pbrA_rev</td>
 +
<td>CTATAGAGTCAAGACATCTCCCACTCTAGAAGCGGCCGCGAAT</td>
 +
</tr>
 +
 +
<tr id="pSB1C3_RFP_rev">
 +
<td>pSB1C3_RFP_rev</td>
 +
<td>CCGCCACTCAACGGGTGCCTGATAATACTAGTAGCGGCCGCTGCA</td>
 +
</tr>
 +
 +
<tr id="pSB1C3_rcnA_rev">
 +
<td>pSB1C3_rcnA_rev</td>
 +
<td>CTTGTTAACCAGGTGTTGCCATGTCTCATCTCTAGAAGCGGCCGCGAAT</td>
 +
</tr>
 +
 +
<tr id="pSB1C3_merR_rev">
 +
<td>pSB1C3_merR_rev</td>
 +
<td>CGGTCTTGCCCGGAGCGCAATGCCGTGACTCTAGAAGCGGCCGCGAAT</td>
 +
</tr>
 +
 +
<tr id="pSB1C3_chrB_rev">
 +
<td>pSB1C3_chrB_rev</td>
 +
<td>CTTTTGAAAAAGGCACTCTGACCGTTTGACTCTAGAAGCGGCCGCGAAT</td>
 +
</tr>
 +
 +
<tr id="pSB1C3_chrP_rev">
 +
<td>pSB1C3_chrP_rev</td>
 +
<td>CAAATGGCAATAGGAATAAGCAATCCTCTAGAAGCGGCCGCGAAT</td>
 +
</tr>
 +
     
  
 
             <tr class="primers-subheading">
 
             <tr class="primers-subheading">
Line 414: Line 709:
 
           <tr id="arsO-fw (amino labeled)">
 
           <tr id="arsO-fw (amino labeled)">
 
                 <td>arsO-fw</td>
 
                 <td>arsO-fw</td>
−
                 <td>ACATTCGTTAAGTCATATATGTTTTTGACTTA</td>
+
                 <td>Aminolink_ACATTCGTTAAGTCATATATGTTTTTGACTTA</td>
 
             </tr>
 
             </tr>
 
           <tr id="arsO-rv (Cy3 labeled)">
 
           <tr id="arsO-rv (Cy3 labeled)">
 
                 <td>arsO-rv</td>
 
                 <td>arsO-rv</td>
−
                 <td>TGTAAGCAATTCAGTATATACAAAAACTGAAT</td>
+
                 <td>Cy3_TGTAAGCAATTCAGTATATACAAAAACTGAAT</td>
 
             </tr>
 
             </tr>
 
           <tr id="SuffixR-fw (amino labeled)">
 
           <tr id="SuffixR-fw (amino labeled)">
 
                 <td>SuffixR-fw</td>
 
                 <td>SuffixR-fw</td>
−
                 <td>CTGCAGCGGCCGCTACTAGTA </td>
+
                 <td>Aminolink_CTGCAGCGGCCGCTACTAGTA </td>
 
             </tr>
 
             </tr>
 
           <tr id="lacO-klein">
 
           <tr id="lacO-klein">
Line 429: Line 724:
 
             </tr>
 
             </tr>
 
           <tr id="lacO-gross">
 
           <tr id="lacO-gross">
−
                 <td>lacO-klein</td>
+
                 <td>lacO-gross</td>
 
                 <td>TGTGGAATTGTGAGCGGATAACAATTACG</td>
 
                 <td>TGTGGAATTGTGAGCGGATAACAATTACG</td>
 
             </tr>
 
             </tr>
Line 480: Line 775:
 
                 <td>GTCTTTCTGCGATCGGTACCC</td>
 
                 <td>GTCTTTCTGCGATCGGTACCC</td>
 
             </tr>
 
             </tr>
 +
          <tr id="lacIhis0418_fw">
 +
                <td>lacIhis0418_fw</td>
 +
                <td>GGGCAGCACCATCACCATCACCATTAATAATACTAGTAGCGGCCGCTGC</td>
 +
            </tr>
 +
          <tr id="lacIhis0418_rv">
 +
                <td>lacIhis0418_rv</td>
 +
                <td>TTATTAATGGTGATGGTGATGGTGCTGCCCGCTTTCCAGTCG</td>
 +
            </tr>
 +
<tr id="VF-Cy3">
 +
<td>VF-Cy3</td>
 +
<td>Cy3-CCACCTGACGTCTAAGAAAC</td>
 +
</tr>
  
  
−
 
+
<tr id="arsO-rv_cy3">
 +
<td>arsO-rv_Cy3</td>
 +
<td>Cy3_TGTAAGCAATTCAGTATATACAAAAACTGAAT</td>
 +
</tr>
  
 
             <tr class="primers-subheading">
 
             <tr class="primers-subheading">
Line 512: Line 822:
 
         </tbody>
 
         </tbody>
 
     </table>
 
     </table>
 +
   
 +
    </div>
  
−
<br>
 
−
<p>The gene-specific part of the primer is written in uppercase letters, while lowercase letters denote an overhang.</p>
 
  
−
</div
+
<div class="row footer">
 +
<div class="col-md-2 col-md-offset-10 text-center">
 +
<a type="button" class="btn btn-default btn-next" href="https://2015.igem.org/Team:Bielefeld-CeBiTec/Organisms"><img src="https://static.igem.org/mediawiki/2015/b/bc/Bielefeld-CeBiTec_notebook_logo.png"><p>Organisms</p></a>
 +
</div>
 +
</div>
 +
 
 +
</div>
 
</body>
 
</body>
  

Latest revision as of 17:00, 18 September 2015

iGEM Bielefeld 2015


Primers

Small in size, but great in performance!

Name Sequence (5´ → 3´)
Standard primers
mRFP1_rev GGACGACCTTCACCTTCACCTTCACC
SB-prep-2Ea ATGAATTCCAGAAATCATCCTTAGCG
SB-prep-3P-1 GCCGCTGCAGTCCGGCAAAAAA
VF CCACCTGACGTCTAAGAA
VR GTATTACCGCCTTTGAGT
Arsenic biosensor
Ars_1_rev TATGACTTAACGAATGTGTAAGTGCAGAGGCGGATAAAACACATCTGAAAATTC
Ars_2_fwd CCTCTGCACTTACACATTCGTTAAGTCATAGGTACTAGTAGCGGCC
Ars_3_fwd CATTCGTTAAGTCATATATGTTTTTGACTTGGTACTAGTAGCGGCC
Ars_3_rev TCAAAAACATATATGACTTAACGAATGTGTCGGATAAAACACATCTGAAAATTC
arsRhis_arsR_fwd TATAGGGAAAGAGGAGAAATACTAGAGATGTCATTTCTGTTACCCATC
arsRhis_arsR2_r CTAGTATTAGTGGTGATGGTGATGATGACTGCAAATGTTCTTACTG
arsRhis_Trp_fwd CATCATCACCATCACCAC
arsRhis_Trp_rev CTCTAGTATTTCTCCTCTTTCC
UTR_arsO_rev CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTAAGTCAAAAACATATATGACTTAACG
UTR_arsR_fwd GTTTAACTTTAAAAAAAAAAAAGAAGGAGATATACATATGTCATTTCTGTTACCCATCC
UTR_arsR_rev CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTCCCTATAGTGAGTCGTATTACTC
UTR_arsR_fwd GTTTAACTTTAAAAAAAAAAAAGAAGGAGATATACATATGTCATTTCTGTTACCCATCC
UTR_arsR_rev CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTCCCTATAGTGAGTCGTATTACTC
CFPS
Mut_gap_UTR_fwd AGAATAATCTATGCGTAAAGGCGAAGAG
pBS1C3_RraA_pre ACAAAGCTCGGAAGTATCGTATTTCATCTCTAGAAGCGGCCGCGAATTCC
pBS1C3_RraA_suf TCAGAAGATCCGCTGGATATTGAATGATACTAGTAGCGGCCGCTGCAG
pBS1C3_RraB_pre TTCTTCCAGTTGTTCCGGGTTTGCCATCTCTAGAAGCGGCCGCGAATTCC
pBS1C3_RraB_suf GAAGACGATGACGGAGTTCGCCACTAATACTAGTAGCGGCCGCTGCAG
pZ9_pT7_rne_fwd GTCCGCAACCTGTTGAGTAACTGCAGGAAGGGGATCCG
pZ9_pT7_rne_rev TAGTATTTCTCCTCTTTCTCTAGTATCTCCCTATAGTGAGTCGTATTAC
rne_qPCR_fwd1 GTGCTCGGCATAAGCCGAAG
rne_qPCR_fwd2 GCATAAGCCGAAGATATCG
rne_qPCR_fwd3 CCGAAGATATCGGTAGAGTT
rne_qPCR_fwd4 CTGCGCTACGGTAGCGAAAC
rne_qPCR_rev2 TTTGCGCCTCGTTATCATCC
rne_to_pZ9_fwd GATACTAGAGAAAGAGGAGAAATACTAGATGAAAAGAATGTTAATCAACGCAACTC
rne_to_pZ9_rev CCTGCAGTTACTCAACAGGTTGCGGACGCGCAG
Rra_purif_fwd TCAGAAGATCCGCTGGATATTGAATGATACTAGAGCCAGGCATCAAA
RraA_fwd ATGAAATACGATACTTCCGAGCTTTG
RraA_purif_rev ACAAAGCTCGGAAGTATCGTATTTCATGGCGCCCTGAAAATACAG
RraA_rev TCATTCAATATCCAGCGGATCTTC
RraB_fwd ATGGCAAACCCGGAACAACTG
RraB_purif_fwd GAAGACGATGACGGAGTTCGCCACTAATACTAGAGCCAGGCATCAAA
RraB_purif_rev TTCTTCCAGTTGTTCCGGGTTTGCCATGGCGCCCTGAAAATACAG
RraB_rev TTAGTGGCGAACTCCGTCATC
SYFP_only_fwd ATGGTTAGCAAGGGCGAAGA
SYFP_only_rev TTATTATTTATACAGCTCATCCATACC
UTR_noT7_rev CCTTCTTTTTTTTTTTTAAAGTTAAAGTTAAACAAAATTATTCTCTAGAAGCGGCCGCGA
UTR_fwd GTTTAACTTTAAAAAAAAAAAAGAAGGAGATATACATATGCGTAAAGGCGAAGAGCTGTTCAC
UTR_rev CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTTCTCCCTATAGTGAGTCGTATTACTCTAGAAG
UTR_RFP_fwd GTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATCTATGGCTTCCTCCGAAGACGTTATC
UTR_SYFP_fwd GTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATCTATGGTTAGCAAGGGCGAAGAAC
Date rape drugs
BlcR_fwd ATGGGCCAAAGGGGCCAAGT
BlcR_rev CTAGTCTTTCTGCGATCGGTACCC
pSB1C3_BlcR_fwd CTTGGGTACCGATCGCAGAAAGACTAGTACTAGTAGCGGCCGCTGCAG
psB_BlcRfwd_new CTTGGGTACCGATCGCAGAAAGACTAATACTAGTAGCGGCCGCTGCAG
pSB1C3_BlcR_rev TTGACACACTTGGCCCCTTTGGCCCATCTAGTATTTCTCCTCTTTCTCTAGTAGCTAGCACAATAC
BlcC_rev TTACGCGTAAAATTCGGGAGCCTTC
BlcC_rev_new TTAGTCTTTCTGCGATCGGTACCC
bclR-rv GTCTTTCTGCGATCGGTACCC
blcR-CBD-fw AAGCTTGGGTACCGATCGCAGAAAGACCCCGGCGCCAATCCGCCCGGT
pro_Blc_fwd ATGCACCTTCCTTGACACACT
pro_Blc_rev CATCGACCTCTCCCGCATTAG
K516030proB_rev GGGCCAAGTGTGTCAAGGAAGGTGCATCTCTAGAAGCGGCCGCGAATTCC
K516030proB_fwd TGAACTCTAATGCGGGAGAGGTCGATGGCTTCCTCCGAAGACGTTAT
BlcB_mut_fwd CCTTTCGAGTTCCGCACCGTTCCCT
BlcB_mut_rev AGGGAACGGTGCGGAACTCGAAAGG
BlcR-L-sfGFP-rv TCGCCTTTACGCATGCCGGTGTCTTTCTGCGATCGGTACCC
BlcR-T7-RBS-fw TAGAGAAAGAGGAGAAATACTAGATGGGCCAAAG
K60_BlcPshort_r GAGTGAACTATGGCTCTAGTAGCTAGCACAATACC
K60_BlcPshort_f GCTAGCTACTAGAGCCATAGTTCACTCTAATGATTC
bclR-rv GTCTTTCTGCGATCGGTACCC
pSB1C3blcOnewfw AAGAAGGCTCCCGAATTTTACGCGTAATACTAGTAGCGGCCGCTGCAG
Pblc_UTR_fwd TCAAGTTCAATTCGTTGCACTCTAATGAATAATTTTGTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATC
Pblc_UTR_rev ATTGAACTTGAATCATTAGAGTGAACTCCCTATAGTGAGTCGTATTACTCTAG
BlcR-His_fwd CGTACTACCATCACCATCACCATCACGGCGCCATGGGCCAAAGGGGCC
BlcR-His_rev CCGTGATGGTGATGGTGATGGTAGTACGACATCTAGTATTTCTCCTCTTTCTCTAGTA
gadR-qRT-fw TCAATCCCCTGTAACGG
gadR-qRT-rev CCAGGCGCACCGAACAAGAC
gadT-qRT-fw ATGAGTCAAACAACAGCAAGCATC
gadT-qRT-rev CTCAACCACCTTCGGATG
oligo_BlcR_51_rev CCCGCATTAGAGTTCAACTAATTGAACTTGAATCATTAGAGTGAACTA TGG
oligo_BlcR_51_fwd _biot Biotin_CCATAGTTCACTCTAATGATTCAAGTTCAATTAGTTGAACTCT AATGCGGG
oligo_BlcR_51_rev_Cy3 Cy3_CCCGCATTAGAGTTCAACTAATTGAACTTGAATCATTAGAGTGAACTA TGG_Cy3
oligo_BlcR_51_fwd _Cy3 Cy3_CCATAGTTCACTCTAATGATTCAAGTTCAATTAGTTGAACTCT AATGCGGG_Cy3
gBlocks
GABA_B_pSB1C3_f ATAAGAAAATACCGACAGGGGGATATTCACTACTAGTAGCGGCCGCTGC
GABA_B_pSB1C3_r CATAAAAAAATCCCAGTCACAGGTGATTGACTCTAGAAGCGGCCGCGA
GABA_R_pSB1C3_f TAGTTTTCAACCCTTTCAGCGGAGGAGCCATCGTACTAGTAGCGGCCGCTGC
GABA_R_pSB1C3_r CTGTGGATTAACACACCACCTACTTTTTAACTCTAGAAGCGGCCGCGA
Trp_sy_pSB1C3_f AAAGAAGTTCATCATCACCATCACCACTAATACTAGTAGCGGCCGCTGC
Trp_sy_pSB1C3_r TCTCCTCTTTCCCTATAGTGAGTCGTATTACTCTAGAAGCGGCCGCGA
Heavy metals
cueR_rev TCACCCTGCCCGATGATGAC
cueR_fwd AGTCCTAGGTATTGTGCTAGCTACTAGAGAAAGAGGAGAAAATGAACATCAGCGATGTAGC
rnR_PstI_fwd CGAATGCGCAGTTTTAC
rnR_PstI_rev GTAAAACTGCGGCGCATTCG
pSB1C3_copAP_fwd TTGACCTTCCCCTTGCTGGAAGGTTTAACCTTTATCACAATTAAAGAGGAGAAATACTAG
pSB1C3_copAP_rev TAAACCTTCCAGCAAGGGGAAGGTCAAAGAAATTAATAAACTCTAGAAGCGGCCGCGAAT
pSB1C3_copA_rev GGTCAAGAAATTAATAAACCAGGCGGGTCTCTAGAAGCGGCCGCGAAT
Chrom2_pSB1C3_fwd AGCTTTGAAAAAGGGACCCTCACCGTATGATACTAGTAGCGGCCGCTGC
Chrom2_pSB1C3_rev AATAAAAGCAAATGGCAATAGGAATAAGCAATCCTCTAGAAGCGGCCGCGA
pSB1C3_cueR_pre GGCTGCTGTCATCATCGGGCAGGGTGACTCTAGAAGCGGCCGCGAAT
pSB1C3_cueR_suf CTAGTAGCTAGCACAATACCTAGGACTGAGCTAGCTGTCAATACTAGTAGCGGCCGCTGCA
pSB1C3_cueR_rev GGTTGTTGTCATCATCGCGCAGGGTAACTCTAGAAGCGGCCGCGAAT
pSB1C3_rcnA_fwd TAGATTAATAGTGCTATGATTTTTTATTGTTAACCAGGTGTTGCCATGTCTCATCTCTAGAAGCGGCCGCGAAT
pSB1C3_rcnA_rev TGAAAAATCATAGCACTATTAATCTAGGGGGGTAGTATCAGGTACTGGGGGGGAGTAATTAAAGAGGAGAAATACTA
pSB1C3_rcnR_rev AGTCCTAGGTATTGTGCTAGCTACTAGAGAAAGAGGAGAAAATGTCTCATACAATCCGTGATA
pSB1C3_rcnR_fwd CGCGGCCGCTTCTAGAGTTACTTGATATATGAATCCAGC
pSB1C3_rcnR_f CTAGTAGCTAGCACAATACCTAGGACTGAGCTAGCTGTAAATACTAGTAGCGGCCGCTGCAGTCC
pSB1C3_rcnR_rev GAAAGTCCTTGATTCGTACATCAAATGACTCTAGAAGCGGCCGCGAAT
pSB1C3_merR_suf CTAGCACAATACCTAGGACTGAGCTAGCTGTCAATAGTAGTAGCGGCCGCTGCAG
pSB1C3_merR_pre GGCCTGGCAAGGTCAGCTAGTCCTTAGCTCTAGAAGCGGCCGCGAAT
rcnA_UTR_fwd CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTTTTCTCCTCTTTTACTCCCCCC
UTR_mod_fwd GTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATCTCTGCGTAAAGGCGAAGAGCTGTTCAC
MerR_fwd TAGCTCAGTCCTAGGTATTGTGCTAGCAAAGAGGAGAAATACTAGATGG
merR_rev CTAAGGCATAGCTGATCTTG
copAP_UTR_rev CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTCTAGTACTTTAAGACAGTTTTG
CrProm_UTR_rev CTTTTTTTTTTTTAAAGTTAAACAAAATTATTGTCGTAGATGTTACTACAATAATGAG
pbrA_UTR_rev CTCCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTCTAGTATTTCTCCTCTTTTTCTCGCGTTGCC
pSB1C3_merT_rev GGATAGCGTAACCTTACTTCCGTACTCATGTACGGAGTCAAGCGATATGGAACTCTAGAAGCGGCCGCGAATTC
merT_omRFP_fwd CTCCGTACATGAGTACGGAAGTAAGGTTACGCTATCCAATCCTACTAGATTAAAGAGGAGAAATAC
1C3_pre_merT_rev CTTACTTCCGTACTCATGTACGGAGTCAAGCGATATGGAACTCTAGAAGCGGCCGCGAATTC
UTR_merT_fwd GAGTACGGAAGTAAGGTTACGCTATCCAATCCTACGACAATAATTTTGTTTAACTTTAAAAAAAAAAAAG
pSB1C3_kPrm_fwd CTAGGACTGAGCTAGCTGTCAATACTAGTAGCGGCCGCTGCA
pSB1C3_kPrm_r GCACCACCGCGCACCCGTCCGATTAACTCTAGAAGCGGCCGCGAAT
pSB1C3-pbrR-fwd CCACCGCGCACCCGTCCGATTAACTCTAGAAGGGCCGCGAAT
pSB1C3-pbrR-f CAATACCTAGGACTGAGCTAGCTGTCAATACTAGTAGCGGCCGCTGCA
pSB1C3-pbrA_rev CTATAGAGTCAAGACATCTCCCACTCTAGAAGCGGCCGCGAAT
pSB1C3_RFP_rev CCGCCACTCAACGGGTGCCTGATAATACTAGTAGCGGCCGCTGCA
pSB1C3_rcnA_rev CTTGTTAACCAGGTGTTGCCATGTCTCATCTCTAGAAGCGGCCGCGAAT
pSB1C3_merR_rev CGGTCTTGCCCGGAGCGCAATGCCGTGACTCTAGAAGCGGCCGCGAAT
pSB1C3_chrB_rev CTTTTGAAAAAGGCACTCTGACCGTTTGACTCTAGAAGCGGCCGCGAAT
pSB1C3_chrP_rev CAAATGGCAATAGGAATAAGCAATCCTCTAGAAGCGGCCGCGAAT
PRIA
T7-RBS-arsR-fw AGGGAGATACTAGAGAAAGAGGAGAAATACTAGATGTCATTTCTGTTACC
arsR-L-sfGFP-rv CAGCTCTTCGCCTTTACGCATGCCGGTTAACTGCAAATGTTCTTAC
L-sfGFP-fw ACCGGCATGCGTAAAGGCGAAGAGCTG
T7-RBS-arsR-rv CTAGTATTTCTCCTCTTTCTCTAGTATC
lacI-sfGFP-fw CCCGACTGGAAAGCGGGCAGACCGGCATGCGTAAAGGCGAAGAGC
lacI-sfGFP-His-rv TAATGGTGATGGTGATGGTGTTTGTACAGTTCATCCATACCATGCGTGATGC
T7-RBS-lacI-rv CTGCCCGCTTTCCAGTCGGGAAACCTGTC
C3-lacI-His-fw AACACCATCACCATCACCATTAATAA
UberHis-arsR-fw CACCATCACCATCACCATTAATAATACTAGAGCCAGGCATCAAA
arsR-UberHis_rv TTATTAATGGTGATGGTGATGGTGTTTGTACAGTTCATCCATACC
BlcR-T7-RBS-fw TAGAGAAAGAGGAGAAATACTAGATGGGCCAAAG
BlcR-L-sfGFP-rv TCGCCTTTACGCATGCCGGTGTCTTTCTGCGATCGGTACCC
arsO-fw Aminolink_ACATTCGTTAAGTCATATATGTTTTTGACTTA
arsO-rv Cy3_TGTAAGCAATTCAGTATATACAAAAACTGAAT
SuffixR-fw Aminolink_CTGCAGCGGCCGCTACTAGTA
lacO-klein ATTTGCTTTGTGAGCGGATAACAAT
lacO-gross TGTGGAATTGTGAGCGGATAACAATTACG
lacI-CBD-fw GACAGGTTTCCCGACTGGAAAGCGGGCAGCCCGGCGCCAATCCGCCCGGTACTACG
lacI-CBD-Vektor-rv GGACTGCAGCGGCCGCTACTAGTATTATTAAAGACACTGGGAGTAGTACG
EndHis-Vektor-lacI-fw TAATAATACTAGTAGCGGCCGCTGCAGTCC
lacI-rv CTGCCCGCTTTCCAGTCGGGAAACC
arsR-dCBD-fw GGGACAGTAAGAACATTTGCAGTTACCCGGCGCCAATCCGCCCGGTAC
CBD-eHisTerm-rv TATTTGATGCCTGGCTCTAGTATTATTAAAGACACTGGGAGTAGTACG
eHis-dTerm-fw TAATAATACTAGAGCCAGGCATCAAA
arsR-rv TAACTGCAAATGTTCTTACTG
bclR-CBD-fw AAGCTTGGGTACCGATCGCAGAAAGACCCCGGCGCCAATCCGCCCGGTACT
CBD-eGFPTerm-rv ATTTGATGCCTGGCTCTAGTATCATCAAAGACACTGGGAGTAGTACG
eGFP-dTerm-fw TGATGATACTAGAGCCAGGCATCAAAT
bclR-rv GTCTTTCTGCGATCGGTACCC
lacIhis0418_fw GGGCAGCACCATCACCATCACCATTAATAATACTAGTAGCGGCCGCTGC
lacIhis0418_rv TTATTAATGGTGATGGTGATGGTGCTGCCCGCTTTCCAGTCG
VF-Cy3 Cy3-CCACCTGACGTCTAAGAAAC
arsO-rv_Cy3 Cy3_TGTAAGCAATTCAGTATATACAAAAACTGAAT
Split primers
Cm_fwd CGGCATCAGCACCTTGTC
Cm_rev TATACGCAAGGCGACAAG

Organisms