Difference between revisions of "Team:Bielefeld-CeBiTec/Primers"

 
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     <table class="table table-hover primers">
 
     <table class="table table-hover primers">
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</tr>
 
</tr>
  
−
<tr id="pSB1C3_copAP_fw">
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<tr id="pSB1C3_copAP_fwd">
−
<td>pSB1C3_copAP_fw</td>
+
<td>pSB1C3_copAP_fwd</td>
 
<td>TTGACCTTCCCCTTGCTGGAAGGTTTAACCTTTATCACAATTAAAGAGGAGAAATACTAG</td>
 
<td>TTGACCTTCCCCTTGCTGGAAGGTTTAACCTTTATCACAATTAAAGAGGAGAAATACTAG</td>
 
</tr>
 
</tr>
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</tr>
 
</tr>
  
−
<tr id="pSB1C3_copA_r">
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<tr id="pSB1C3_copA_rev">
−
<td>pSB1C3_copA_r</td>
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<td>pSB1C3_copA_rev</td>
 
<td>GGTCAAGAAATTAATAAACCAGGCGGGTCTCTAGAAGCGGCCGCGAAT</td>
 
<td>GGTCAAGAAATTAATAAACCAGGCGGGTCTCTAGAAGCGGCCGCGAAT</td>
 
</tr>
 
</tr>
  
−
<tr id="Chrom2_pSB1C3_f">
+
<tr id="Chrom2_pSB1C3_fwd">
−
<td>Chrom2_pSB1C3_f</td>
+
<td>Chrom2_pSB1C3_fwd</td>
 
<td>AGCTTTGAAAAAGGGACCCTCACCGTATGATACTAGTAGCGGCCGCTGC</td>
 
<td>AGCTTTGAAAAAGGGACCCTCACCGTATGATACTAGTAGCGGCCGCTGC</td>
 
</tr>
 
</tr>
  
−
<tr id="Chrom2_pSB1C3_r">
+
<tr id="Chrom2_pSB1C3_rev">
−
<td>Chrom2_pSB1C3_r</td>
+
<td>Chrom2_pSB1C3_rev</td>
 
<td>AATAAAAGCAAATGGCAATAGGAATAAGCAATCCTCTAGAAGCGGCCGCGA</td>
 
<td>AATAAAAGCAAATGGCAATAGGAATAAGCAATCCTCTAGAAGCGGCCGCGA</td>
 
</tr>
 
</tr>
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</tr>
 
</tr>
  
−
<tr id="pSB1C3_cueR_r">
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<tr id="pSB1C3_cueR_rev">
−
<td>pSB1C3_cueR_r</td>
+
<td>pSB1C3_cueR_rev</td>
 
<td>GGTTGTTGTCATCATCGCGCAGGGTAACTCTAGAAGCGGCCGCGAAT</td>
 
<td>GGTTGTTGTCATCATCGCGCAGGGTAACTCTAGAAGCGGCCGCGAAT</td>
 
</tr>
 
</tr>
  
−
<tr id="pSB1C3_rcnA_fw">
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<tr id="pSB1C3_rcnA_fwd">
−
<td>pSB1C3_rcnA_fw</td>
+
<td>pSB1C3_rcnA_fwd</td>
 
<td>TAGATTAATAGTGCTATGATTTTTTATTGTTAACCAGGTGTTGCCATGTCTCATCTCTAGAAGCGGCCGCGAAT</td>
 
<td>TAGATTAATAGTGCTATGATTTTTTATTGTTAACCAGGTGTTGCCATGTCTCATCTCTAGAAGCGGCCGCGAAT</td>
 
</tr>
 
</tr>
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</tr>
 
</tr>
  
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<tr id="pSB1C3_rcnR_r">
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<tr id="pSB1C3_rcnR_rev">
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<td>pSB1C3_rcnR_r</td>
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<td>pSB1C3_rcnR_rev</td>
 
<td>GAAAGTCCTTGATTCGTACATCAAATGACTCTAGAAGCGGCCGCGAAT</td>
 
<td>GAAAGTCCTTGATTCGTACATCAAATGACTCTAGAAGCGGCCGCGAAT</td>
 
</tr>
 
</tr>
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<td>pSB1C3_merR_pre</td>
 
<td>pSB1C3_merR_pre</td>
 
<td>GGCCTGGCAAGGTCAGCTAGTCCTTAGCTCTAGAAGCGGCCGCGAAT</td>
 
<td>GGCCTGGCAAGGTCAGCTAGTCCTTAGCTCTAGAAGCGGCCGCGAAT</td>
−
</tr>
 
−
 
−
<tr id="RFP_fwd">
 
−
<td>RFP_fwd</td>
 
−
<td><p></p></td>
 
−
</tr>
 
−
 
−
<tr id="kPrm_fwd">
 
−
<td>kPrm_fwd</td>
 
−
<td><p></td>
 
 
</tr>
 
</tr>
  
  
−
<tr id="rcnA_UTR_fw">
+
<tr id="rcnA_UTR_fwd">
−
<td>rcnA_UTR_fw</td>
+
<td>rcnA_UTR_fwd</td>
 
<td>CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTTTTCTCCTCTTTTACTCCCCCC</td>
 
<td>CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTTTTCTCCTCTTTTACTCCCCCC</td>
 
</tr>
 
</tr>
  
−
<tr id="UTR_mod_fw">
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<tr id="UTR_mod_fwd">
−
<td>UTR_mod_fw</td>
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<td>UTR_mod_fwd</td>
 
<td>GTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATCTCTGCGTAAAGGCGAAGAGCTGTTCAC</td>
 
<td>GTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATCTCTGCGTAAAGGCGAAGAGCTGTTCAC</td>
 
</tr>
 
</tr>
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</tr>
 
</tr>
  
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<tr id="1C3_pre_merT_rv">
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<tr id="1C3_pre_merT_rev">
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<td>1C3_pre_merT_rv</td>
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<td>1C3_pre_merT_rev</td>
 
<td>CTTACTTCCGTACTCATGTACGGAGTCAAGCGATATGGAACTCTAGAAGCGGCCGCGAATTC</td>
 
<td>CTTACTTCCGTACTCATGTACGGAGTCAAGCGATATGGAACTCTAGAAGCGGCCGCGAATTC</td>
 
</tr>
 
</tr>
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</tr>
 
</tr>
  
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<tr id="pSB1C3_kPrm_rev">
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<tr id="pSB1C3_kPrm_fwd">
−
<td>pSB1C3_kPrm_rev</td>
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<td>pSB1C3_kPrm_fwd</td>
 
<td>CTAGGACTGAGCTAGCTGTCAATACTAGTAGCGGCCGCTGCA</td>
 
<td>CTAGGACTGAGCTAGCTGTCAATACTAGTAGCGGCCGCTGCA</td>
 
</tr>
 
</tr>
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</tr>
 
</tr>
  
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<tr id="pSB1C3-pbrA_r">
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<tr id="pSB1C3-pbrA_rev">
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<td>pSB1C3-pbrA_r</td>
+
<td>pSB1C3-pbrA_rev</td>
 
<td>CTATAGAGTCAAGACATCTCCCACTCTAGAAGCGGCCGCGAAT</td>
 
<td>CTATAGAGTCAAGACATCTCCCACTCTAGAAGCGGCCGCGAAT</td>
 
</tr>
 
</tr>
  
−
<tr id="pSB1C3_RFP_fw">
+
<tr id="pSB1C3_RFP_rev">
−
<td>pSB1C3_RFP_fw</td>
+
<td>pSB1C3_RFP_rev</td>
 
<td>CCGCCACTCAACGGGTGCCTGATAATACTAGTAGCGGCCGCTGCA</td>
 
<td>CCGCCACTCAACGGGTGCCTGATAATACTAGTAGCGGCCGCTGCA</td>
 
</tr>
 
</tr>
  
−
<tr id="pSB1C3_rcnA_r">
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<tr id="pSB1C3_rcnA_rev">
−
<td>pSB1C3_rcnA_r</td>
+
<td>pSB1C3_rcnA_rev</td>
 
<td>CTTGTTAACCAGGTGTTGCCATGTCTCATCTCTAGAAGCGGCCGCGAAT</td>
 
<td>CTTGTTAACCAGGTGTTGCCATGTCTCATCTCTAGAAGCGGCCGCGAAT</td>
 
</tr>
 
</tr>
  
−
<tr id="pSB1C3_merR_r">
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<tr id="pSB1C3_merR_rev">
−
<td>pSB1C3_merR_r</td>
+
<td>pSB1C3_merR_rev</td>
 
<td>CGGTCTTGCCCGGAGCGCAATGCCGTGACTCTAGAAGCGGCCGCGAAT</td>
 
<td>CGGTCTTGCCCGGAGCGCAATGCCGTGACTCTAGAAGCGGCCGCGAAT</td>
 
</tr>
 
</tr>
  
−
<tr id="pSB1C3_chrB_r">
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<tr id="pSB1C3_chrB_rev">
−
<td>pSB1C3_chrB_r</td>
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<td>pSB1C3_chrB_rev</td>
 
<td>CTTTTGAAAAAGGCACTCTGACCGTTTGACTCTAGAAGCGGCCGCGAAT</td>
 
<td>CTTTTGAAAAAGGCACTCTGACCGTTTGACTCTAGAAGCGGCCGCGAAT</td>
 
</tr>
 
</tr>
  
−
<tr id="pSB1C3_chrP_r">
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<tr id="pSB1C3_chrP_rev">
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<td>pSB1C3_chrP_r</td>
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<td>pSB1C3_chrP_rev</td>
 
<td>CAAATGGCAATAGGAATAAGCAATCCTCTAGAAGCGGCCGCGAAT</td>
 
<td>CAAATGGCAATAGGAATAAGCAATCCTCTAGAAGCGGCCGCGAAT</td>
 
</tr>
 
</tr>
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         </tbody>
 
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<a type="button" class="btn btn-default btn-next" href="https://2015.igem.org/Team:Bielefeld-CeBiTec/Organisms"><img src="https://static.igem.org/mediawiki/2015/b/bc/Bielefeld-CeBiTec_notebook_logo.png"><p>Organisms</p></a>
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</body>
 
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Latest revision as of 17:00, 18 September 2015

iGEM Bielefeld 2015


Primers

Small in size, but great in performance!

Name Sequence (5´ → 3´)
Standard primers
mRFP1_rev GGACGACCTTCACCTTCACCTTCACC
SB-prep-2Ea ATGAATTCCAGAAATCATCCTTAGCG
SB-prep-3P-1 GCCGCTGCAGTCCGGCAAAAAA
VF CCACCTGACGTCTAAGAA
VR GTATTACCGCCTTTGAGT
Arsenic biosensor
Ars_1_rev TATGACTTAACGAATGTGTAAGTGCAGAGGCGGATAAAACACATCTGAAAATTC
Ars_2_fwd CCTCTGCACTTACACATTCGTTAAGTCATAGGTACTAGTAGCGGCC
Ars_3_fwd CATTCGTTAAGTCATATATGTTTTTGACTTGGTACTAGTAGCGGCC
Ars_3_rev TCAAAAACATATATGACTTAACGAATGTGTCGGATAAAACACATCTGAAAATTC
arsRhis_arsR_fwd TATAGGGAAAGAGGAGAAATACTAGAGATGTCATTTCTGTTACCCATC
arsRhis_arsR2_r CTAGTATTAGTGGTGATGGTGATGATGACTGCAAATGTTCTTACTG
arsRhis_Trp_fwd CATCATCACCATCACCAC
arsRhis_Trp_rev CTCTAGTATTTCTCCTCTTTCC
UTR_arsO_rev CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTAAGTCAAAAACATATATGACTTAACG
UTR_arsR_fwd GTTTAACTTTAAAAAAAAAAAAGAAGGAGATATACATATGTCATTTCTGTTACCCATCC
UTR_arsR_rev CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTCCCTATAGTGAGTCGTATTACTC
UTR_arsR_fwd GTTTAACTTTAAAAAAAAAAAAGAAGGAGATATACATATGTCATTTCTGTTACCCATCC
UTR_arsR_rev CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTCCCTATAGTGAGTCGTATTACTC
CFPS
Mut_gap_UTR_fwd AGAATAATCTATGCGTAAAGGCGAAGAG
pBS1C3_RraA_pre ACAAAGCTCGGAAGTATCGTATTTCATCTCTAGAAGCGGCCGCGAATTCC
pBS1C3_RraA_suf TCAGAAGATCCGCTGGATATTGAATGATACTAGTAGCGGCCGCTGCAG
pBS1C3_RraB_pre TTCTTCCAGTTGTTCCGGGTTTGCCATCTCTAGAAGCGGCCGCGAATTCC
pBS1C3_RraB_suf GAAGACGATGACGGAGTTCGCCACTAATACTAGTAGCGGCCGCTGCAG
pZ9_pT7_rne_fwd GTCCGCAACCTGTTGAGTAACTGCAGGAAGGGGATCCG
pZ9_pT7_rne_rev TAGTATTTCTCCTCTTTCTCTAGTATCTCCCTATAGTGAGTCGTATTAC
rne_qPCR_fwd1 GTGCTCGGCATAAGCCGAAG
rne_qPCR_fwd2 GCATAAGCCGAAGATATCG
rne_qPCR_fwd3 CCGAAGATATCGGTAGAGTT
rne_qPCR_fwd4 CTGCGCTACGGTAGCGAAAC
rne_qPCR_rev2 TTTGCGCCTCGTTATCATCC
rne_to_pZ9_fwd GATACTAGAGAAAGAGGAGAAATACTAGATGAAAAGAATGTTAATCAACGCAACTC
rne_to_pZ9_rev CCTGCAGTTACTCAACAGGTTGCGGACGCGCAG
Rra_purif_fwd TCAGAAGATCCGCTGGATATTGAATGATACTAGAGCCAGGCATCAAA
RraA_fwd ATGAAATACGATACTTCCGAGCTTTG
RraA_purif_rev ACAAAGCTCGGAAGTATCGTATTTCATGGCGCCCTGAAAATACAG
RraA_rev TCATTCAATATCCAGCGGATCTTC
RraB_fwd ATGGCAAACCCGGAACAACTG
RraB_purif_fwd GAAGACGATGACGGAGTTCGCCACTAATACTAGAGCCAGGCATCAAA
RraB_purif_rev TTCTTCCAGTTGTTCCGGGTTTGCCATGGCGCCCTGAAAATACAG
RraB_rev TTAGTGGCGAACTCCGTCATC
SYFP_only_fwd ATGGTTAGCAAGGGCGAAGA
SYFP_only_rev TTATTATTTATACAGCTCATCCATACC
UTR_noT7_rev CCTTCTTTTTTTTTTTTAAAGTTAAAGTTAAACAAAATTATTCTCTAGAAGCGGCCGCGA
UTR_fwd GTTTAACTTTAAAAAAAAAAAAGAAGGAGATATACATATGCGTAAAGGCGAAGAGCTGTTCAC
UTR_rev CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTTCTCCCTATAGTGAGTCGTATTACTCTAGAAG
UTR_RFP_fwd GTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATCTATGGCTTCCTCCGAAGACGTTATC
UTR_SYFP_fwd GTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATCTATGGTTAGCAAGGGCGAAGAAC
Date rape drugs
BlcR_fwd ATGGGCCAAAGGGGCCAAGT
BlcR_rev CTAGTCTTTCTGCGATCGGTACCC
pSB1C3_BlcR_fwd CTTGGGTACCGATCGCAGAAAGACTAGTACTAGTAGCGGCCGCTGCAG
psB_BlcRfwd_new CTTGGGTACCGATCGCAGAAAGACTAATACTAGTAGCGGCCGCTGCAG
pSB1C3_BlcR_rev TTGACACACTTGGCCCCTTTGGCCCATCTAGTATTTCTCCTCTTTCTCTAGTAGCTAGCACAATAC
BlcC_rev TTACGCGTAAAATTCGGGAGCCTTC
BlcC_rev_new TTAGTCTTTCTGCGATCGGTACCC
bclR-rv GTCTTTCTGCGATCGGTACCC
blcR-CBD-fw AAGCTTGGGTACCGATCGCAGAAAGACCCCGGCGCCAATCCGCCCGGT
pro_Blc_fwd ATGCACCTTCCTTGACACACT
pro_Blc_rev CATCGACCTCTCCCGCATTAG
K516030proB_rev GGGCCAAGTGTGTCAAGGAAGGTGCATCTCTAGAAGCGGCCGCGAATTCC
K516030proB_fwd TGAACTCTAATGCGGGAGAGGTCGATGGCTTCCTCCGAAGACGTTAT
BlcB_mut_fwd CCTTTCGAGTTCCGCACCGTTCCCT
BlcB_mut_rev AGGGAACGGTGCGGAACTCGAAAGG
BlcR-L-sfGFP-rv TCGCCTTTACGCATGCCGGTGTCTTTCTGCGATCGGTACCC
BlcR-T7-RBS-fw TAGAGAAAGAGGAGAAATACTAGATGGGCCAAAG
K60_BlcPshort_r GAGTGAACTATGGCTCTAGTAGCTAGCACAATACC
K60_BlcPshort_f GCTAGCTACTAGAGCCATAGTTCACTCTAATGATTC
bclR-rv GTCTTTCTGCGATCGGTACCC
pSB1C3blcOnewfw AAGAAGGCTCCCGAATTTTACGCGTAATACTAGTAGCGGCCGCTGCAG
Pblc_UTR_fwd TCAAGTTCAATTCGTTGCACTCTAATGAATAATTTTGTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATC
Pblc_UTR_rev ATTGAACTTGAATCATTAGAGTGAACTCCCTATAGTGAGTCGTATTACTCTAG
BlcR-His_fwd CGTACTACCATCACCATCACCATCACGGCGCCATGGGCCAAAGGGGCC
BlcR-His_rev CCGTGATGGTGATGGTGATGGTAGTACGACATCTAGTATTTCTCCTCTTTCTCTAGTA
gadR-qRT-fw TCAATCCCCTGTAACGG
gadR-qRT-rev CCAGGCGCACCGAACAAGAC
gadT-qRT-fw ATGAGTCAAACAACAGCAAGCATC
gadT-qRT-rev CTCAACCACCTTCGGATG
oligo_BlcR_51_rev CCCGCATTAGAGTTCAACTAATTGAACTTGAATCATTAGAGTGAACTA TGG
oligo_BlcR_51_fwd _biot Biotin_CCATAGTTCACTCTAATGATTCAAGTTCAATTAGTTGAACTCT AATGCGGG
oligo_BlcR_51_rev_Cy3 Cy3_CCCGCATTAGAGTTCAACTAATTGAACTTGAATCATTAGAGTGAACTA TGG_Cy3
oligo_BlcR_51_fwd _Cy3 Cy3_CCATAGTTCACTCTAATGATTCAAGTTCAATTAGTTGAACTCT AATGCGGG_Cy3
gBlocks
GABA_B_pSB1C3_f ATAAGAAAATACCGACAGGGGGATATTCACTACTAGTAGCGGCCGCTGC
GABA_B_pSB1C3_r CATAAAAAAATCCCAGTCACAGGTGATTGACTCTAGAAGCGGCCGCGA
GABA_R_pSB1C3_f TAGTTTTCAACCCTTTCAGCGGAGGAGCCATCGTACTAGTAGCGGCCGCTGC
GABA_R_pSB1C3_r CTGTGGATTAACACACCACCTACTTTTTAACTCTAGAAGCGGCCGCGA
Trp_sy_pSB1C3_f AAAGAAGTTCATCATCACCATCACCACTAATACTAGTAGCGGCCGCTGC
Trp_sy_pSB1C3_r TCTCCTCTTTCCCTATAGTGAGTCGTATTACTCTAGAAGCGGCCGCGA
Heavy metals
cueR_rev TCACCCTGCCCGATGATGAC
cueR_fwd AGTCCTAGGTATTGTGCTAGCTACTAGAGAAAGAGGAGAAAATGAACATCAGCGATGTAGC
rnR_PstI_fwd CGAATGCGCAGTTTTAC
rnR_PstI_rev GTAAAACTGCGGCGCATTCG
pSB1C3_copAP_fwd TTGACCTTCCCCTTGCTGGAAGGTTTAACCTTTATCACAATTAAAGAGGAGAAATACTAG
pSB1C3_copAP_rev TAAACCTTCCAGCAAGGGGAAGGTCAAAGAAATTAATAAACTCTAGAAGCGGCCGCGAAT
pSB1C3_copA_rev GGTCAAGAAATTAATAAACCAGGCGGGTCTCTAGAAGCGGCCGCGAAT
Chrom2_pSB1C3_fwd AGCTTTGAAAAAGGGACCCTCACCGTATGATACTAGTAGCGGCCGCTGC
Chrom2_pSB1C3_rev AATAAAAGCAAATGGCAATAGGAATAAGCAATCCTCTAGAAGCGGCCGCGA
pSB1C3_cueR_pre GGCTGCTGTCATCATCGGGCAGGGTGACTCTAGAAGCGGCCGCGAAT
pSB1C3_cueR_suf CTAGTAGCTAGCACAATACCTAGGACTGAGCTAGCTGTCAATACTAGTAGCGGCCGCTGCA
pSB1C3_cueR_rev GGTTGTTGTCATCATCGCGCAGGGTAACTCTAGAAGCGGCCGCGAAT
pSB1C3_rcnA_fwd TAGATTAATAGTGCTATGATTTTTTATTGTTAACCAGGTGTTGCCATGTCTCATCTCTAGAAGCGGCCGCGAAT
pSB1C3_rcnA_rev TGAAAAATCATAGCACTATTAATCTAGGGGGGTAGTATCAGGTACTGGGGGGGAGTAATTAAAGAGGAGAAATACTA
pSB1C3_rcnR_rev AGTCCTAGGTATTGTGCTAGCTACTAGAGAAAGAGGAGAAAATGTCTCATACAATCCGTGATA
pSB1C3_rcnR_fwd CGCGGCCGCTTCTAGAGTTACTTGATATATGAATCCAGC
pSB1C3_rcnR_f CTAGTAGCTAGCACAATACCTAGGACTGAGCTAGCTGTAAATACTAGTAGCGGCCGCTGCAGTCC
pSB1C3_rcnR_rev GAAAGTCCTTGATTCGTACATCAAATGACTCTAGAAGCGGCCGCGAAT
pSB1C3_merR_suf CTAGCACAATACCTAGGACTGAGCTAGCTGTCAATAGTAGTAGCGGCCGCTGCAG
pSB1C3_merR_pre GGCCTGGCAAGGTCAGCTAGTCCTTAGCTCTAGAAGCGGCCGCGAAT
rcnA_UTR_fwd CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTTTTCTCCTCTTTTACTCCCCCC
UTR_mod_fwd GTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATCTCTGCGTAAAGGCGAAGAGCTGTTCAC
MerR_fwd TAGCTCAGTCCTAGGTATTGTGCTAGCAAAGAGGAGAAATACTAGATGG
merR_rev CTAAGGCATAGCTGATCTTG
copAP_UTR_rev CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTCTAGTACTTTAAGACAGTTTTG
CrProm_UTR_rev CTTTTTTTTTTTTAAAGTTAAACAAAATTATTGTCGTAGATGTTACTACAATAATGAG
pbrA_UTR_rev CTCCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTCTAGTATTTCTCCTCTTTTTCTCGCGTTGCC
pSB1C3_merT_rev GGATAGCGTAACCTTACTTCCGTACTCATGTACGGAGTCAAGCGATATGGAACTCTAGAAGCGGCCGCGAATTC
merT_omRFP_fwd CTCCGTACATGAGTACGGAAGTAAGGTTACGCTATCCAATCCTACTAGATTAAAGAGGAGAAATAC
1C3_pre_merT_rev CTTACTTCCGTACTCATGTACGGAGTCAAGCGATATGGAACTCTAGAAGCGGCCGCGAATTC
UTR_merT_fwd GAGTACGGAAGTAAGGTTACGCTATCCAATCCTACGACAATAATTTTGTTTAACTTTAAAAAAAAAAAAG
pSB1C3_kPrm_fwd CTAGGACTGAGCTAGCTGTCAATACTAGTAGCGGCCGCTGCA
pSB1C3_kPrm_r GCACCACCGCGCACCCGTCCGATTAACTCTAGAAGCGGCCGCGAAT
pSB1C3-pbrR-fwd CCACCGCGCACCCGTCCGATTAACTCTAGAAGGGCCGCGAAT
pSB1C3-pbrR-f CAATACCTAGGACTGAGCTAGCTGTCAATACTAGTAGCGGCCGCTGCA
pSB1C3-pbrA_rev CTATAGAGTCAAGACATCTCCCACTCTAGAAGCGGCCGCGAAT
pSB1C3_RFP_rev CCGCCACTCAACGGGTGCCTGATAATACTAGTAGCGGCCGCTGCA
pSB1C3_rcnA_rev CTTGTTAACCAGGTGTTGCCATGTCTCATCTCTAGAAGCGGCCGCGAAT
pSB1C3_merR_rev CGGTCTTGCCCGGAGCGCAATGCCGTGACTCTAGAAGCGGCCGCGAAT
pSB1C3_chrB_rev CTTTTGAAAAAGGCACTCTGACCGTTTGACTCTAGAAGCGGCCGCGAAT
pSB1C3_chrP_rev CAAATGGCAATAGGAATAAGCAATCCTCTAGAAGCGGCCGCGAAT
PRIA
T7-RBS-arsR-fw AGGGAGATACTAGAGAAAGAGGAGAAATACTAGATGTCATTTCTGTTACC
arsR-L-sfGFP-rv CAGCTCTTCGCCTTTACGCATGCCGGTTAACTGCAAATGTTCTTAC
L-sfGFP-fw ACCGGCATGCGTAAAGGCGAAGAGCTG
T7-RBS-arsR-rv CTAGTATTTCTCCTCTTTCTCTAGTATC
lacI-sfGFP-fw CCCGACTGGAAAGCGGGCAGACCGGCATGCGTAAAGGCGAAGAGC
lacI-sfGFP-His-rv TAATGGTGATGGTGATGGTGTTTGTACAGTTCATCCATACCATGCGTGATGC
T7-RBS-lacI-rv CTGCCCGCTTTCCAGTCGGGAAACCTGTC
C3-lacI-His-fw AACACCATCACCATCACCATTAATAA
UberHis-arsR-fw CACCATCACCATCACCATTAATAATACTAGAGCCAGGCATCAAA
arsR-UberHis_rv TTATTAATGGTGATGGTGATGGTGTTTGTACAGTTCATCCATACC
BlcR-T7-RBS-fw TAGAGAAAGAGGAGAAATACTAGATGGGCCAAAG
BlcR-L-sfGFP-rv TCGCCTTTACGCATGCCGGTGTCTTTCTGCGATCGGTACCC
arsO-fw Aminolink_ACATTCGTTAAGTCATATATGTTTTTGACTTA
arsO-rv Cy3_TGTAAGCAATTCAGTATATACAAAAACTGAAT
SuffixR-fw Aminolink_CTGCAGCGGCCGCTACTAGTA
lacO-klein ATTTGCTTTGTGAGCGGATAACAAT
lacO-gross TGTGGAATTGTGAGCGGATAACAATTACG
lacI-CBD-fw GACAGGTTTCCCGACTGGAAAGCGGGCAGCCCGGCGCCAATCCGCCCGGTACTACG
lacI-CBD-Vektor-rv GGACTGCAGCGGCCGCTACTAGTATTATTAAAGACACTGGGAGTAGTACG
EndHis-Vektor-lacI-fw TAATAATACTAGTAGCGGCCGCTGCAGTCC
lacI-rv CTGCCCGCTTTCCAGTCGGGAAACC
arsR-dCBD-fw GGGACAGTAAGAACATTTGCAGTTACCCGGCGCCAATCCGCCCGGTAC
CBD-eHisTerm-rv TATTTGATGCCTGGCTCTAGTATTATTAAAGACACTGGGAGTAGTACG
eHis-dTerm-fw TAATAATACTAGAGCCAGGCATCAAA
arsR-rv TAACTGCAAATGTTCTTACTG
bclR-CBD-fw AAGCTTGGGTACCGATCGCAGAAAGACCCCGGCGCCAATCCGCCCGGTACT
CBD-eGFPTerm-rv ATTTGATGCCTGGCTCTAGTATCATCAAAGACACTGGGAGTAGTACG
eGFP-dTerm-fw TGATGATACTAGAGCCAGGCATCAAAT
bclR-rv GTCTTTCTGCGATCGGTACCC
lacIhis0418_fw GGGCAGCACCATCACCATCACCATTAATAATACTAGTAGCGGCCGCTGC
lacIhis0418_rv TTATTAATGGTGATGGTGATGGTGCTGCCCGCTTTCCAGTCG
VF-Cy3 Cy3-CCACCTGACGTCTAAGAAAC
arsO-rv_Cy3 Cy3_TGTAAGCAATTCAGTATATACAAAAACTGAAT
Split primers
Cm_fwd CGGCATCAGCACCTTGTC
Cm_rev TATACGCAAGGCGACAAG

Organisms