Difference between revisions of "Team:SCUT/Safety"

 
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    <h1 id="safetyTitle">SAFETY</h1>
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    <h2 style="color:#00b4ed">Laboratory Safety</h2>
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    <p>In our project we worked with BL1 materials E. coli for cloning and BL2 materials Human Embryonic Kidney 293 cells for transfection. All students in our team received lab-specific training and students who worked with BL2 material received additional blood-borne pathogens training. Before we start our experiment, we made a detailed protocol and communicated with our instructor to ensure that we are followed the safety standard in laboratory. Before each experiment we wore protective equipment such as lab coat, gloves and gauze mask. To keep ourselves and our sample safe all the experiments of BL2 materials were handled in biosafety cabinets .After experiment, BL2 material devil liquor was handled with decontamination bleach mixture under ultraviolet rays for 24 hours. All laboratory chemicals were handled in accordance with the information on their MSDS to keep us safe.</p>
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<h2>Safety</h2>
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<h3 class="service">What have you done to stay safe in the lab ?</h3>
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<p class="clear">Firstly ,we don’t use any pathogenic microorganism ,secondly ,we use gloves and mask when necessary ,thirdly, all the vectors and genetic microorganism and Cd2+ solution won’t be abandoned directly into environment unless they are treated properly.</p>
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<h3 class="service"> What potential damage may be caused when the genetic microorganism are used in industrial environment?</h3>
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<p class="clear">Because the Cd2+ solution can only be absorbed rather than be broken down, if the genetic microorganism which have absorbed Cd2+ are not recycled,it will release Cd2+ to natural environment sooner or later. At the same time ,the genetic microorganisms may cause a potential damage to the natural ecosystem.</p>
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<h3 class="service">How do you dispose the waste Escherichia coli?</h3>
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    <h2 style="color:#e5004f">Biological Parts</h2>
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<p class="clear">The E.coli we used is not harmful to human beings. But in order to protect everyone in the laboratory and prevent the E.coli from polluting the environment. We take the boil way to kill the E.coli before it is abandoned. </p>
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    <p>We construct 2 basic parts that encoding internal protein in human body and designed 3 scilencing devices . The most hazardous biological parts in our parts are the scilencing devices since they encode the hairpin RNA that do not exsist in human body and they may cause potential risks. We transfected the scilencing devices into HEK293 to scilence the PDE5A gene. To make our parts safer we designed a hypoxia responsive switch, only under hypoxia situation the down stream parts will work. In our experiment we used lentivirus to packaged our plasmids so that all the transfection experiments were handled in II biological safety cabinet.</p>
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<h3 class="service">How do you dispose the medium with cadmium ion?</h3>
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<p class="clear">Since Cadmium ion is a highly toxic heavy metal, our project utilizes the Cadmium ion that is low contribution. In order to keep the cadmium ion pollution under control, we add the phosphate buffer to the cadmium ion solution to form precipitation. Then, we would send the cadmium ion precipitation to the environment agency of SCUT.</p>
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<h3 class="service">What will we do to improve the problem mentioned above ?</h3>
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<p class="clear">We have come up with 2 possible ways to solve it. First of all, we tend to add a adhesion molecule mechanism which is controlled by PCadA to make it easier to get down in the bottom of the water as the microorganism begin to absorb Cd2+.So the Secondly, we add auxotrophic genes in the relevant auxotrophic bacteria which are controlled by PCadA so that the genetic microorganisms can live in our polluted water rather than natural environment.</p>
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<h3 class="service"> Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?</h3>
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<p class="clear">We believe using specific strains designed specifically for labwork will be safer. It is possible to genetically modify the organisms so that, they are not able to grow outside the lab environment. There probably is not a way to build some biobricks that can be modified in order to make them safer since their function will be the cause of safety issues.</p>
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<h3>About Us</h3>
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<p class="percent-two-third">In 2015, we SCUT teams won top ten innovative and entrepreneurial team set up by SCUT.Because of the strong support of the college, our team is being on the right track, and increasing understanding of the subject and experience.  </p>
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<h3 style="margin-left:20px;">Thanks</h3>
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<ul>
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<li>Zhang Zhenwu,Prof. Guo Shouqian,Dr. Li, Dr. Li Cheng,Dr. Wang Meng,Chen Kejie</li>
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<li>Guangzhou Municipal Environmental Protection Bureau<br/>
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</a></li>
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<p>COPYRIGHT &copy;2015-SCUT</p>
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Latest revision as of 17:04, 18 September 2015

Team:SCUT

Safety

What have you done to stay safe in the lab ?

Firstly ,we don’t use any pathogenic microorganism ,secondly ,we use gloves and mask when necessary ,thirdly, all the vectors and genetic microorganism and Cd2+ solution won’t be abandoned directly into environment unless they are treated properly.

What potential damage may be caused when the genetic microorganism are used in industrial environment?

Because the Cd2+ solution can only be absorbed rather than be broken down, if the genetic microorganism which have absorbed Cd2+ are not recycled,it will release Cd2+ to natural environment sooner or later. At the same time ,the genetic microorganisms may cause a potential damage to the natural ecosystem.

How do you dispose the waste Escherichia coli?

The E.coli we used is not harmful to human beings. But in order to protect everyone in the laboratory and prevent the E.coli from polluting the environment. We take the boil way to kill the E.coli before it is abandoned.

How do you dispose the medium with cadmium ion?

Since Cadmium ion is a highly toxic heavy metal, our project utilizes the Cadmium ion that is low contribution. In order to keep the cadmium ion pollution under control, we add the phosphate buffer to the cadmium ion solution to form precipitation. Then, we would send the cadmium ion precipitation to the environment agency of SCUT.

What will we do to improve the problem mentioned above ?

We have come up with 2 possible ways to solve it. First of all, we tend to add a adhesion molecule mechanism which is controlled by PCadA to make it easier to get down in the bottom of the water as the microorganism begin to absorb Cd2+.So the Secondly, we add auxotrophic genes in the relevant auxotrophic bacteria which are controlled by PCadA so that the genetic microorganisms can live in our polluted water rather than natural environment.

Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?

We believe using specific strains designed specifically for labwork will be safer. It is possible to genetically modify the organisms so that, they are not able to grow outside the lab environment. There probably is not a way to build some biobricks that can be modified in order to make them safer since their function will be the cause of safety issues.

About Us

In 2015, we SCUT teams won top ten innovative and entrepreneurial team set up by SCUT.Because of the strong support of the college, our team is being on the right track, and increasing understanding of the subject and experience.

Thanks

  • Zhang Zhenwu,Prof. Guo Shouqian,Dr. Li, Dr. Li Cheng,Dr. Wang Meng,Chen Kejie
  • Guangzhou Municipal Environmental Protection Bureau

COPYRIGHT ©2015-SCUT