Difference between revisions of "Team:DTU-Denmark"

Line 3: Line 3:
 
     <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1, user-scalable=no">
 
     <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1, user-scalable=no">
 
     <meta http-equiv="X-UA-Compatible" content="IE=edge">
 
     <meta http-equiv="X-UA-Compatible" content="IE=edge">
 +
   
 +
   
 +
    <link href="/Team:DTU-Denmark/loadphpcss?action=raw&ctype=text/css" rel="stylesheet">
 +
    <link href="/Team:DTU-Denmark/bootstrapcss?action=raw&ctype=text/css" rel="stylesheet">
 +
    <link href="/Team:DTU-Denmark/font-awesomecss?action=raw&ctype=text/css" rel="stylesheet">
 +
    <link href="/Team:DTU-Denmark/wikicss?action=raw&ctype=text/css" rel="stylesheet">
 +
    <link href="/Team:DTU-Denmark/timelinecss?action=raw&ctype=text/css" rel="stylesheet">
 +
    <link href="/Team:DTU-Denmark/menufixcss?action=raw&ctype=text/css" rel="stylesheet">
  
−
      <link rel=stylesheet href="/Team:DTU-Denmark/loadphpcss?action=raw&ctype=text/css">
+
   
−
      <link rel=stylesheet href="/Team:DTU-Denmark/bootstrapcss?action=raw&ctype=text/css">
+
−
      <link rel=stylesheet href="/Team:DTU-Denmark/font-awesomecss?action=raw&ctype=text/css">
+
−
      <link rel=stylesheet href="/Team:DTU-Denmark/wikicss?action=raw&ctype=text/css">
+
−
      <link rel=stylesheet href="/Team:DTU-Denmark/timelinecss?action=raw&ctype=text/css">
+
−
      <link rel=stylesheet href="/Team:DTU-Denmark/menufixcss?action=raw&ctype=text/css">
+
−
 
+
 
     <script type="text/javascript" src="/Team:DTU-Denmark/jqueryjs?action=raw&ctype=text/javascript"></script>
 
     <script type="text/javascript" src="/Team:DTU-Denmark/jqueryjs?action=raw&ctype=text/javascript"></script>
 
     <script type="text/javascript" src="/Team:DTU-Denmark/bootstrapjs?action=raw&ctype=text/javascript"></script>
 
     <script type="text/javascript" src="/Team:DTU-Denmark/bootstrapjs?action=raw&ctype=text/javascript"></script>
 
     <script type="text/javascript" src="/Team:DTU-Denmark/jqueryeasingjs?action=raw&ctype=text/javascript"></script>
 
     <script type="text/javascript" src="/Team:DTU-Denmark/jqueryeasingjs?action=raw&ctype=text/javascript"></script>
 
     <script type="text/javascript" src="/Team:DTU-Denmark/wikijs?action=raw&ctype=text/javascript"></script>
 
     <script type="text/javascript" src="/Team:DTU-Denmark/wikijs?action=raw&ctype=text/javascript"></script>
−
 
 
     <!-- MathJax (LaTeX for the web) -->
 
     <!-- MathJax (LaTeX for the web) -->
 
     <script src="https://2015.igem.org/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_SVG"></script>
 
     <script src="https://2015.igem.org/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_SVG"></script>
Line 37: Line 38:
 
     <nav class="navbar navbar-default navbar-fixed-top" role="navigation">
 
     <nav class="navbar navbar-default navbar-fixed-top" role="navigation">
 
       <!-- Brand and toggle get grouped for better mobile display -->
 
       <!-- Brand and toggle get grouped for better mobile display -->
−
       <div class="container">
+
       <div class="container-fluid">
 
         <div class="navbar-header">
 
         <div class="navbar-header">
 
           <button type="button" class="navbar-toggle" data-toggle="collapse" data-target="#nav-collapse">
 
           <button type="button" class="navbar-toggle" data-toggle="collapse" data-target="#nav-collapse">
 
             <span class="sr-only">Toggle navigation</span>
 
             <span class="sr-only">Toggle navigation</span>
 
             <i class="fa fa-bars"></i>
 
             <i class="fa fa-bars"></i>
−
            <!--span class="icon-bar"></span>
 
−
            <span class="icon-bar"></span>
 
−
            <span class="icon-bar"></span-->
 
 
           </button>
 
           </button>
−
           <a class="navbar-brand navimg" href="#"><img src="https://static.igem.org/mediawiki/2015/b/b7/DTU-Denmark_dtulogo.png"></a>
+
           <a class="navbar-brand navimg" href="#"><img src="/wiki/images/b/b7/DTU-Denmark_dtulogo.png"></a>
 
         </div>
 
         </div>
−
 
 
 
         <!-- Collect the nav links, forms, and other content for toggling -->
 
         <!-- Collect the nav links, forms, and other content for toggling -->
 
         <div class="collapse navbar-collapse" id="nav-collapse">
 
         <div class="collapse navbar-collapse" id="nav-collapse">
−
           <ul class="nav navbar-nav navbar-right hidden-lg hidden-xs">
+
           <ul class="nav navbar-nav">
−
            <li>
+
<li class="dropdown active">
−
              <a href="https://igem.org" target="_blank" class="navimg">
+
<a href="/Team:DTU-Denmark"
−
                <img src="https://static.igem.org/mediawiki/2015/a/af/DTU-Denmark_igemlogo.png">
+
  >Home
−
              </a>
+
                                          </a></li>
−
            </li>
+
<li class="dropdown">
−
          </ul>
+
<a href="/Team:DTU-Denmark/attributions"
−
          <ul class="nav navbar-nav navbar-right hidden-xs">
+
  >Team and Attributions
−
             <li>
+
                                          </a></li>
 +
<li class="dropdown">
 +
<a href="/Team:DTU-Denmark/Project"
 +
  class="dropdown-toggle"
 +
  data-toggle="dropdown"
 +
  role="button"
 +
  aria-expanded="false">Project
 +
                                          <span class="caret"></span></a><ul class="dropdown-menu" role="menu">
 +
<li class="dropdown">
 +
<a href="/Team:DTU-Denmark/Project/Overview"
 +
  >Overview
 +
                                          </a></li>
 +
<li class="dropdown">
 +
<a href="/Team:DTU-Denmark/Procject/Background"
 +
  >Background
 +
                                          </a></li>
 +
<li class="dropdown">
 +
<a href="/Team:DTU-Denmark/Project/MAGE"
 +
  >MAGE Subtilis
 +
                                          </a></li>
 +
<li class="dropdown">
 +
<a href="/Team:DTU-Denmark/Project/Surfactin"
 +
  >Surfactin
 +
                                          </a></li>
 +
<li class="dropdown">
 +
<a href="/Team:DTU-Denmark/Project/Tyrocidine"
 +
  >Tyrocidine
 +
                                          </a></li>
 +
<li class="dropdown">
 +
<a href="/Team:DTU-Denmark/Project/Chip"
 +
  >Lab-on-a-disc
 +
                                          </a></li>
 +
<li class="dropdown">
 +
<a href="/Team:DTU-Denmark/Project/Intein"
 +
  >Intein
 +
                                          </a></li>
 +
<li class="dropdown">
 +
<a href="/Team:DTU-Denmark/Project/Detection"
 +
  >Detection of NRP
 +
                                          </a></li></ul></li>
 +
<li class="dropdown">
 +
<a href="/Team:DTU-Denmark/Practices"
 +
  >Human Practices
 +
                                          </a></li>
 +
<li class="dropdown">
 +
<a href="/Team:DTU-Denmark/Part_Collection"
 +
  >Parts
 +
                                          </a></li>
 +
<li class="dropdown">
 +
<a href="/Team:DTU-Denmark/Journal"
 +
  >Journal
 +
                                          </a></li>
 +
<li class="dropdown">
 +
<a href="/Team:DTU-Denmark/Software"
 +
  >Software
 +
                                          </a></li>
 +
<li class="dropdown">
 +
<a href="/Team:DTU-Denmark/Achievements"
 +
  >Achievements
 +
                                          </a></li>
 +
             <li class="hidden-xs">
 
               <a href="http://facebook.com/dtubiobuilders" data-toggle="tooltip" data-placement="bottom" title="Follow us on Facebook">
 
               <a href="http://facebook.com/dtubiobuilders" data-toggle="tooltip" data-placement="bottom" title="Follow us on Facebook">
−
                 <i class="fa fa-facebook"></i>
+
                 <i class="fa fa-facebook"></i><span class="visible-xs"> Follow us on Facebook</span>
 
               </a>
 
               </a>
 
             </li>
 
             </li>
−
             <li>
+
             <li class="visible-xs"><a href="http://facebook.com/dtubiobuilders"><i class="fa fa-facebook-square"></i> Follow us on Facebook</a></li>
 +
            <li class="hidden-xs">
 
               <a href="https://twitter.com/IGEM_DTU" data-toggle="tooltip" data-placement="bottom" title="Follow us on Twitter">
 
               <a href="https://twitter.com/IGEM_DTU" data-toggle="tooltip" data-placement="bottom" title="Follow us on Twitter">
 
                 <i class="fa fa-twitter"></i>
 
                 <i class="fa fa-twitter"></i>
 
               </a>
 
               </a>
 
             </li>
 
             </li>
−
          </ul>
+
             <li class="visible-xs"><a href="https://twitter.com/IGEM_DTU"><i class="fa fa-twitter-square"></i> Follow us on Twitter</a></li>
−
          <ul class="nav nav-justified">
+
             <li class="hidden-lg">
−
           
+
−
             <li class="active" >
+
−
              <a href="/Team:DTU-Denmark">Home</a>
+
−
            </li>
+
−
           
+
−
            <li >
+
−
              <a href="/Team:DTU-Denmark/Team">Team</a>
+
−
            </li>
+
−
           
+
−
            <li >
+
−
              <a href="/Team:DTU-Denmark/Project">Project</a>
+
−
            </li>
+
−
           
+
−
            <li >
+
−
              <a href="/Team:DTU-Denmark/Modeling">Modeling</a>
+
−
            </li>
+
−
           
+
−
            <li >
+
−
              <a href="/Team:DTU-Denmark/Software">Software</a>
+
−
            </li>
+
−
           
+
−
            <li >
+
−
              <a href="/Team:DTU-Denmark/Practices">Human Practices</a>
+
−
            </li>
+
−
           
+
−
             <li >
+
−
              <a href="/Team:DTU-Denmark/Journal">Journal</a>
+
−
            </li>
+
−
           
+
−
            <li >
+
−
              <a href="/Team:DTU-Denmark/Part_Collection">Parts</a>
+
−
            </li>
+
−
           
+
−
            <li >
+
−
              <a href="/Team:DTU-Denmark/Achievements">Achievements</a>
+
−
            </li>
+
−
           
+
−
          </ul>
+
−
          <ul class="nav navbar-nav navbar-right visible-xs">
+
−
            <li>
+
 
               <a href="https://igem.org" target="_blank" class="navimg">
 
               <a href="https://igem.org" target="_blank" class="navimg">
−
                 <img src="https://static.igem.org/mediawiki/2015/a/af/DTU-Denmark_igemlogo.png">
+
                 <img src="/wiki/images/a/af/DTU-Denmark_igemlogo.png">
 
               </a>
 
               </a>
 
             </li>
 
             </li>
−
            <li><a href="http://facebook.com/dtubiobuilders"><i class="fa fa-facebook-square"></i> Follow us on Facebook</a></li>
 
−
            <li><a href="https://twitter.com/IGEM_DTU"><i class="fa fa-twitter-square"></i> Follow us on Twitter</a></li>
 
 
           </ul>
 
           </ul>
 
         </div><!-- /.navbar-collapse -->
 
         </div><!-- /.navbar-collapse -->
Line 122: Line 138:
 
     </nav>
 
     </nav>
 
     <nav class="navbar navbar-default extrabar hidden-xs hidden" data-spy="affix">
 
     <nav class="navbar navbar-default extrabar hidden-xs hidden" data-spy="affix">
−
       <div class="container" id="scrollspy">
+
       <div class="container-fluid" id="scrollspy">
−
         <ul class="nav nav-justified">
+
         <ul class="nav navbar-nav">
 
           <li><a class="page-scroll" href="#Project-description">Project description</a></li><li><a class="page-scroll" href="#Social-Media">Social Media</a></li><li><a class="page-scroll" href="#Overview">Overview</a></li><li><a class="page-scroll" href="#Sitemap">Sitemap</a></li>
 
           <li><a class="page-scroll" href="#Project-description">Project description</a></li><li><a class="page-scroll" href="#Social-Media">Social Media</a></li><li><a class="page-scroll" href="#Overview">Overview</a></li><li><a class="page-scroll" href="#Sitemap">Sitemap</a></li>
 
         </ul>
 
         </ul>
 
       </div>
 
       </div>
 
     </nav>
 
     </nav>
−
     <div class="jumbotron hero" style="background-image: url(https://static.igem.org/mediawiki/2015/0/0b/DTU-Denmark_hero.jpg)">
+
     <div class="jumbotron hero bg-hero">
 
       <div class="igem-logo visible-lg">
 
       <div class="igem-logo visible-lg">
 
         <a href="https://igem.org" target="_blank">
 
         <a href="https://igem.org" target="_blank">
−
           <img src="https://static.igem.org/mediawiki/2015/a/af/DTU-Denmark_igemlogo.png">
+
           <img src="/wiki/images/a/af/DTU-Denmark_igemlogo.png">
 
         </a>
 
         </a>
 
       </div>
 
       </div>
−
       <div class="container">
+
       <div class="container-fluid">
 
         <div class="row hero-text">
 
         <div class="row hero-text">
 
           <div class="col-md-12">
 
           <div class="col-md-12">
Line 185: Line 201:
 
           <div class="col-md-12">
 
           <div class="col-md-12">
 
             Scroll down for more<br>
 
             Scroll down for more<br>
−
             <a href="#" class="btn btn-circle btn-transparent page-scroll">
+
             <a href="#Project-description" class="btn btn-circle btn-transparent page-scroll">
 
               <i class="fa fa-angle-double-down"></i>
 
               <i class="fa fa-angle-double-down"></i>
 
             </a>
 
             </a>
Line 193: Line 209:
 
     </div>
 
     </div>
 
      
 
      
−
       
+
 
<div id="Project-description">
 
<div id="Project-description">
 
   <div class="container">
 
   <div class="container">
Line 200: Line 216:
 
         Project description
 
         Project description
 
       </h1>
 
       </h1>
−
       <p style="text-align: center;"><span class="fa fa-headphones" style="color:rgb(0, 0, 0);font-size:72px;"></span></p>
+
       <p style="text-align: center;"><span style="font-size:72px;"><span class="fa fa-headphones " style="color:#000000;"></span></span></p>
  
 
<p style="text-align: center;">Tune in and listen to The Synthesizers podcast explaining our project in 20 seconds, 5 minutes, or 10 minutes</p>
 
<p style="text-align: center;">Tune in and listen to The Synthesizers podcast explaining our project in 20 seconds, 5 minutes, or 10 minutes</p>
  
−
<p style="text-align: justify;">Non-ribosomal peptides have important anti-bacterial, anti-cancer, and immunosuppressive biological activities. They are synthesized by modular, high molecular weight enzymes that assemble more than 500 different amino acid substrates in an assembly line manner. For this reason, synthetic biologists have tried to engineer these proteins and to switch modules to create analogs and novel natural products, but with little success. Despite being modular, the interactions between modules have evolved to be highly specific, making synthetic Non-Ribosomal Peptide Synthases (NRPS) a challenge to engineer. Instead of switching modules we introduced a recombination system targeting oligo integration in Bacillus subtilis. We used the recombineering system to alter the active sites determining substrate specificity, thereby creating variants of antibiotics. Our focus was the tyrocidine antibiotic, which cannot be used intravenously due to its toxicity. Our goal is to create new analogs through multiplex automated genome engineering to reduce toxicity.</p>
+
<p>Non-ribosomal peptides have important anti-bacterial, anti-cancer, and immunosuppressive biological activities. They are synthesized by modular, high molecular weight enzymes that assemble more than 500 different amino acid substrates in an assembly line manner. For this reason, synthetic biologists have tried to engineer these proteins and to switch modules to create analogs and novel natural products, but with little success. Despite being modular, the interactions between modules have evolved to be highly specific, making synthetic Non-Ribosomal Peptide Synthases (NRPS) a challenge to engineer. Instead of switching modules we introduced a recombination system targeting oligo integration in Bacillus subtilis. We used the recombineering system to alter the active sites determining substrate specificity, thereby creating variants of antibiotics. Our focus was the tyrocidine antibiotic, which cannot be used intravenously due to its toxicity. Our goal is to create new analogs through multiplex automated genome engineering to reduce toxicity.</p>
 +
 
 +
<p>&nbsp;</p>
  
 
     </div>
 
     </div>
 
   </div>
 
   </div>
 
</div>
 
</div>
−
   
+
−
       
+
 
<div id="Social-Media">
 
<div id="Social-Media">
 
   <div class="container">
 
   <div class="container">
Line 217: Line 235:
 
         Social Media
 
         Social Media
 
       </h1>
 
       </h1>
−
       <p><a data-widget-id="631150747221139456" href="https://twitter.com/iGEM_DTU">Tweets by @iGEM_DTU</a> <script>!function(d,s,id){var js,fjs=d.getElementsByTagName(s)[0],p=/^http:/.test(d.location)?'http':'https';if(!d.getElementById(id)){js=d.createElement(s);js.id=id;js.src=p+"://platform.twitter.com/widgets.js";fjs.parentNode.insertBefore(js,fjs);}}(document,"script","twitter-wjs");</script></p>
+
       <div class="col-md-6">
−
 
+
<p><a class="twitter-timeline" data-widget-id="631150747221139456" href="https://twitter.com/iGEM_DTU">Tweets by @iGEM_DTU</a> <script>!function(d,s,id){var js,fjs=d.getElementsByTagName(s)[0],p=/^http:/.test(d.location)?'http':'https';if(!d.getElementById(id)){js=d.createElement(s);js.id=id;js.src=p+"://platform.twitter.com/widgets.js";fjs.parentNode.insertBefore(js,fjs);}}(document,"script","twitter-wjs");</script></p>
 +
</div>
 +
<div class="col-md-6">
 +
<div class="fb-page" data-adapt-container-width="true" data-height="600" data-hide-cover="false" data-href="https://www.facebook.com/dtubiobuilders" data-show-facepile="true" data-show-posts="true" data-small-header="false">
 +
<div class="fb-xfbml-parse-ignore">
 
<blockquote cite="https://www.facebook.com/dtubiobuilders"><a href="https://www.facebook.com/dtubiobuilders">DTU Biobuilders</a></blockquote>
 
<blockquote cite="https://www.facebook.com/dtubiobuilders"><a href="https://www.facebook.com/dtubiobuilders">DTU Biobuilders</a></blockquote>
 +
</div>
 +
</div>
 +
</div>
  
 
     </div>
 
     </div>
 
   </div>
 
   </div>
 
</div>
 
</div>
−
   
+
−
       
+
 
<div id="Overview">
 
<div id="Overview">
 
   <div class="container">
 
   <div class="container">
Line 237: Line 262:
 
   </div>
 
   </div>
 
</div>
 
</div>
−
   
+
−
       
+
 
<div id="Sitemap">
 
<div id="Sitemap">
 
   <div class="container">
 
   <div class="container">
Line 245: Line 270:
 
         Sitemap
 
         Sitemap
 
       </h1>
 
       </h1>
−
       <p>Missing</p>
+
       <p>TODO</p>
  
 
     </div>
 
     </div>
 
   </div>
 
   </div>
 
</div>
 
</div>
−
   
+
−
     <footer class="container">
+
     <div class="container">
 
       <div class="row">
 
       <div class="row">
 
         <div class="col-md-2 col-md-offset-2">
 
         <div class="col-md-2 col-md-offset-2">
 
           <a href="http://www.dtu.dk" target="_blank">
 
           <a href="http://www.dtu.dk" target="_blank">
−
             <img src="https://static.igem.org/mediawiki/2015/b/b7/DTU-Denmark_dtulogo.png" height="130px">
+
             <img src="/wiki/images/b/b7/DTU-Denmark_dtulogo.png" height="130px">
 
           </a>
 
           </a>
 
         </div>
 
         </div>
Line 271: Line 296:
 
         <div class="col-md-2">
 
         <div class="col-md-2">
 
           <a href="https://igem.org" style="float:left" target="_blank">
 
           <a href="https://igem.org" style="float:left" target="_blank">
−
               <img src="https://static.igem.org/mediawiki/2015/a/af/DTU-Denmark_igemlogo.png" height="90px">
+
               <img src="/wiki/images/a/af/DTU-Denmark_igemlogo.png" height="90px">
 
           </a>
 
           </a>
 
         </div>
 
         </div>
 
       </div>
 
       </div>
−
     </footer>
+
     </div>
 
     <div class="sponsors">
 
     <div class="sponsors">
−
       <div class="container">
+
       <div class="container-fluid">
 
         <div class="row">
 
         <div class="row">
 
            
 
            
 
           <div class="sponsorlogo">
 
           <div class="sponsorlogo">
 
             <a href="http://www.lundbeckfonden.com/" target="_blank">
 
             <a href="http://www.lundbeckfonden.com/" target="_blank">
−
               <img src="">
+
               <img src="/wiki/images/e/e2/DTU-Denmark_Lundbeckfonden.jpg">
 
             </a>
 
             </a>
 
           </div>
 
           </div>
Line 288: Line 313:
 
           <div class="sponsorlogo">
 
           <div class="sponsorlogo">
 
             <a href="http://www.ottomoensted.dk" target="_blank">
 
             <a href="http://www.ottomoensted.dk" target="_blank">
−
               <img src="">
+
               <img src="/wiki/images/3/35/DTU-Denmark_Ottomoensted.png">
 
             </a>
 
             </a>
 
           </div>
 
           </div>
Line 294: Line 319:
 
           <div class="sponsorlogo">
 
           <div class="sponsorlogo">
 
             <a href="http://www.novonordiskfonden.dk/en" target="_blank">
 
             <a href="http://www.novonordiskfonden.dk/en" target="_blank">
−
               <img src="">
+
               <img src="/wiki/images/5/5c/DTU-Denmark_NovoNordiskFonden_logo.png">
 
             </a>
 
             </a>
 
           </div>
 
           </div>
Line 300: Line 325:
 
           <div class="sponsorlogo">
 
           <div class="sponsorlogo">
 
             <a href="https://dk.vwr.com/" target="_blank">
 
             <a href="https://dk.vwr.com/" target="_blank">
−
               <img src="">
+
               <img src="/wiki/images/e/ea/DTU-Denmark_VWR.jpg">
 
             </a>
 
             </a>
 
           </div>
 
           </div>
Line 306: Line 331:
 
           <div class="sponsorlogo">
 
           <div class="sponsorlogo">
 
             <a href="http://frisenette.dk" target="_blank">
 
             <a href="http://frisenette.dk" target="_blank">
−
               <img src="">
+
               <img src="/wiki/images/c/c8/DTU-Denmark_Frisenette.jpg">
 
             </a>
 
             </a>
 
           </div>
 
           </div>
Line 312: Line 337:
 
           <div class="sponsorlogo">
 
           <div class="sponsorlogo">
 
             <a href="http://www.in-vitro.dk/" target="_blank">
 
             <a href="http://www.in-vitro.dk/" target="_blank">
−
               <img src="">
+
               <img src="/wiki/images/7/7c/DTU-Denmark_In_Vitro.jpg">
 
             </a>
 
             </a>
 
           </div>
 
           </div>
Line 318: Line 343:
 
           <div class="sponsorlogo">
 
           <div class="sponsorlogo">
 
             <a href="https://dk.fishersci.com/dk/" target="_blank">
 
             <a href="https://dk.fishersci.com/dk/" target="_blank">
−
               <img src="">
+
               <img src="/wiki/images/b/b4/DTU-Denmark_Fisher_Scientific.jpg">
 
             </a>
 
             </a>
 
           </div>
 
           </div>
Line 324: Line 349:
 
           <div class="sponsorlogo">
 
           <div class="sponsorlogo">
 
             <a href="http://us.akg.com/akg-homepage-us.html" target="_blank">
 
             <a href="http://us.akg.com/akg-homepage-us.html" target="_blank">
−
               <img src="">
+
               <img src="/wiki/images/9/9d/DTU-Denmark_AKG.png">
 
             </a>
 
             </a>
 
           </div>
 
           </div>
Line 330: Line 355:
 
           <div class="sponsorlogo">
 
           <div class="sponsorlogo">
 
             <a href="http://www.macrogen.com/" target="_blank">
 
             <a href="http://www.macrogen.com/" target="_blank">
−
               <img src="">
+
               <img src="/wiki/images/1/11/DTU-Denmark_Marcogen.jpg">
 +
            </a>
 +
          </div>
 +
         
 +
          <div class="sponsorlogo">
 +
            <a href="http://www.snapgene.com/" target="_blank">
 +
              <img src="/wiki/images/5/58/DTU-Denmark_Snapgene.png">
 
             </a>
 
             </a>
 
           </div>
 
           </div>
Line 336: Line 367:
 
           <div class="sponsorlogo">
 
           <div class="sponsorlogo">
 
             <a href="https://www.neb.com/" target="_blank">
 
             <a href="https://www.neb.com/" target="_blank">
−
               <img src="">
+
               <img src="/wiki/images/e/e0/DTU-Denmark_NEB_logo.png">
 
             </a>
 
             </a>
 
           </div>
 
           </div>

Revision as of 17:09, 18 September 2015

Technical University of Denmark

The Synthesizer

Scroll down for more

Project description

Tune in and listen to The Synthesizers podcast explaining our project in 20 seconds, 5 minutes, or 10 minutes

Non-ribosomal peptides have important anti-bacterial, anti-cancer, and immunosuppressive biological activities. They are synthesized by modular, high molecular weight enzymes that assemble more than 500 different amino acid substrates in an assembly line manner. For this reason, synthetic biologists have tried to engineer these proteins and to switch modules to create analogs and novel natural products, but with little success. Despite being modular, the interactions between modules have evolved to be highly specific, making synthetic Non-Ribosomal Peptide Synthases (NRPS) a challenge to engineer. Instead of switching modules we introduced a recombination system targeting oligo integration in Bacillus subtilis. We used the recombineering system to alter the active sites determining substrate specificity, thereby creating variants of antibiotics. Our focus was the tyrocidine antibiotic, which cannot be used intravenously due to its toxicity. Our goal is to create new analogs through multiplex automated genome engineering to reduce toxicity.

 

Social Media

DTU Biobuilders

Overview

Overview stuff

Sitemap

TODO

Technical University of Denmark
Department of Systems Biology
Søltofts Plads 221
2800 Kgs. Lyngby
Denmark
P: +45 45 25 25 25
M: dtu-igem-2015@googlegroups.com