Difference between revisions of "Team:San Andres/Parts"

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{{San_Andres}}
 
 
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<h2> Part Documentation</h2>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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  <title></title>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<img src="https://static.igem.org/mediawiki/2015/0/09/Firma_800.jpg"
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<h4>Note</h4>
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<title>wiki 2</title>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<h4>Adding parts to the registry</h4>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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width: 100%;
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<h4>What information do I need to start putting my parts on the Registry?</h4>
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<p>The information needed to initially create a part on the Registry is:</p>
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</style><!--navigation menu -->
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<table class="gwd-table-nb4m editable" width="100%">
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  <tbody>
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    </tr>
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    <tr heigth="75px">
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      <td style="border: 2px solid black;"
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onmouseover="this.bgColor='#ffba00'"
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onmouseout="this.bgColor='#fcff00'" align="center"
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bgcolor="#fcff00" height="45">
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      <a href="https://2015.igem.org/Team:San_Andres"
 +
style="color: rgb(0, 0, 0);">Home </a> </td>
 +
      <td style="border: 2px solid black;"
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onmouseover="this.bgColor='#ffba00'"
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onmouseout="this.bgColor='#fcff00'" align="center"
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bgcolor="#fcff00" height="45">
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      <a href="https://2015.igem.org/Team:San_Andres/Team"
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style="color: rgb(0, 0, 0);"> Team </a> </td>
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      <a href="https://2015.igem.org/Team:San_Andres/Description"
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style="color: rgb(0, 0, 0);"> Project </a>
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      </td>
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      <td style="border: 2px solid black;"
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onmouseover="this.bgColor='#ffba00'"
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onmouseout="this.bgColor='#fcff00'" align="center"
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bgcolor="#fcff00" height="45">
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      <a href="https://2015.igem.org/Team:San_Andres/Notebook"
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style="color: rgb(0, 0, 0);"> Statistics</a>
 +
      </td>
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      <td style="border: 2px solid black;"
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onmouseover="this.bgColor='#ffba00'"
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      <a href="https://2015.igem.org/Team:San_Andres/Experiments"
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style="color: rgb(0, 0, 0);"> Enzymes</a>
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      </td>
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      <td style="border: 2px solid black;"
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      <a href="https://2015.igem.org/Team:San_Andres/Parts"
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style="color: rgb(0, 0, 0);"> Parts</a>
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      </td>
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      <td style="border: 2px solid black;"
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onmouseover="this.bgColor='#ffba00'"
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      <a href="https://2015.igem.org/Team:San_Andres/Notebook"
 +
style="color: rgb(0, 0, 0);"> Metodology</a>
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      </td>
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      <a href="https://2015.igem.org/Team:San_Andres/Results"
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style="color: rgb(0, 0, 0);"> Results </a>
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      </td>
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      <a href="https://2015.igem.org/Team:San_Andres/Collaborations"
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style="color: rgb(0, 0, 0);"> Future Projections </a>
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      </td>
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      <td align="center">
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      <a href="https://igem.org/Team_List?year=2015"><img
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src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"
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width="55"></a></td>
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<div class="gwd-div-2ed9"></div>
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<div class="gwd-div-7xzo gwd-a-1thu">
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<h1>Parts&nbsp;</h1>
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<big>Throughout the project we started to learn the fundamental
 +
principles of synthetic biology to get to work on our plasmid with
 +
which we want to see gluten degradation via the enzyme Kumamax. For
 +
this we going to insert in an e. coli the parts (Biobricks) needed to
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make our future bacteria can degrade gluten. The parts are:<br>
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</big>
 
<ul>
 
<ul>
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<li>Part Name</li>
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  <li><big><span
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<li>Part type</li>
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style="font-family: 'Arial','sans-serif';"><strong
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<li>Creator</li>
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style="font-weight: bold;">Promoter (BBa_J23119</strong><span
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<li>Sequence</li>
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style="font-weight: bold;">)</span>: </span>Constitutive
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<li>Short Description (60 characters on what the DNA does)</li>
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promoter (which works permanently) that is give in the relative
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<li>Long Description (Longer description of what the DNA does)</li>
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fluorescence of these plasmids in the TG1 strain grown in LB medium.</big></li>
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<li>Design considerations</li>
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  <li><big><strong style="font-weight: bold;">RBS
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(BBa_K1084103)</strong>: Synthetic RBS with uplifting sequence.</big></li>
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  <li><big><strong><span
 +
style="font-family: 'Arial','sans-serif';">Vector:<span
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style="font-weight: normal;"> pET29B+</span></span></strong></big></li>
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  <li><big><strong><span
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style="font-family: 'Arial','sans-serif';">Coding Region:
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KumaMax (BBa_K590087):</span></strong> It degrades gluten,
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celiac disease leading cause. Enzyme generated by rational mutation for
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the active site of it.</big></li>
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  <li><big><strong><span
 +
style="font-family: 'Arial','sans-serif';">Reporter: RFP
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(BBa_J04450):</span></strong><span
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style="font-family: Arial,sans-serif;"> Red f</span>luorescence
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protein.</big></li>
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  <li><big><span style="font-family: Arial,sans-serif;"><strong>Terminator
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(BBa_B0015):</strong> </span>Dual terminator consisting of
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the B0010 and B0012 parties. It serves to give greater efficiency in
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transcription</big>.</li>
 
</ul>
 
</ul>
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<div style="text-align: center;"><img alt="File:1.png"
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<p>
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  src="https://static.igem.org/mediawiki/2015/4/4a/1.png" height="68"
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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width="312"><br>
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<img alt="File:2.png"
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src="https://static.igem.org/mediawiki/2015/f/fb/2.png" height="276"
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width="698"><br>
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<div style="text-align: left;"><big>This is a
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graphic model of as it has be our plasmid where we can visualize the
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promoter, the RBS, the enzyme KumaMax, the RFP and the terminator,
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<h4>Inspiration</h4>
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joined by means of prefixes and suffixes that indicate the locations of
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<p>We have a created a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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court.</big><br>
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<br>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<div style="text-align: center;"><img
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<ul>
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alt="File:Plasmido.jpg"
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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src="https://static.igem.org/mediawiki/2015/thumb/c/ce/Plasmido.jpg/664px-Plasmido.jpg"
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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height="600" width="664"></div>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
+
</div>
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</ul>
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</div>
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<big><br>
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</big>
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<div style="text-align: center;"><br>
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<h4>Part Table </h4>
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<br>
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</html>
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</div>
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<groupparts>iGEM015 Example</groupparts>
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Revision as of 20:09, 5 July 2015

wiki 2

Home Team Project Statistics Enzymes Parts Metodology Results Future Projections

Parts 

Throughout the project we started to learn the fundamental principles of synthetic biology to get to work on our plasmid with which we want to see gluten degradation via the enzyme Kumamax. For this we going to insert in an e. coli the parts (Biobricks) needed to make our future bacteria can degrade gluten. The parts are:
  • Promoter (BBa_J23119): Constitutive promoter (which works permanently) that is give in the relative fluorescence of these plasmids in the TG1 strain grown in LB medium.
  • RBS (BBa_K1084103): Synthetic RBS with uplifting sequence.
  • Vector: pET29B+
  • Coding Region: KumaMax (BBa_K590087): It degrades gluten, celiac disease leading cause. Enzyme generated by rational mutation for the active site of it.
  • Reporter: RFP (BBa_J04450): Red fluorescence protein.
  • Terminator (BBa_B0015): Dual terminator consisting of the B0010 and B0012 parties. It serves to give greater efficiency in transcription.
File:1.png
File:2.png
This is a graphic model of as it has be our plasmid where we can visualize the promoter, the RBS, the enzyme KumaMax, the RFP and the terminator, joined by means of prefixes and suffixes that indicate the locations of court.

File:Plasmido.jpg