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| | <style> | | <style> |
| − | .ui.menu { margin-top: 10px !important;} | + | .sponsor img, |
| − | | + | .ufscar img { |
| − | .slides img { | + | height: 150px; |
| − | width: 100%;
| + | } |
| − | height: auto;
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| − | } | + | h1.ui.header { |
| − | .fluid.container .text.container { | + | padding: 0 0; |
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| + | margin-bottom: 0; |
| − | } | + | } |
| − | .ufscar.slider img, .sponsor.slider img { | + | |
| − | height: 150px;
| + | h2.ui.header { |
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| | + | .segment .text.container { |
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| | </style> | | </style> |
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| − | <div class="ui hidden divider"></div> | + | <div class="ui hidden divider"></div> |
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| − | <div class="ui blue vertical center aligned inverted segment"> | + | <div class="ui vertical center aligned inverted segment"> |
| − | <div class="ui fluid container"> | + | <div class="ui fluid container"> |
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| − | <div class="banner slider">
| + | <img class="ui fluid image" src=" https:/ /static.igem. org/ mediawiki/ 2015/ e/ e1/ Banner- 1.jpg" alt=""> |
| − | <div>
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| − | <img class ="ui fluid image" src=" img/ banner-2. jpg" alt=""> | + | |
| − | </div>
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| − | <div>
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| − | <img class ="ui fluid image" src="img/banner-1.jpg" alt="">
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| − | </div>
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| − | <div>
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| − | <img class ="ui fluid image" src="img/banner-3.jpg" alt="">
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| − | <img class ="ui fluid image" src="img/banner-4.jpg" alt="">
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| − | <div class="ui hidden divider"></div>
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| − | <div class="ui text container"> | + | <div class="ui hidden divider"></div> |
| − | <h1 class="ui inverted header">
| + | |
| − | UFSCar - Brasil
| + | |
| − | </h1>
| + | |
| − | <h2>People helping people</h2>
| + | |
| − | <div class="ui huge inverted primary basic button">See our project <i class="right arrow icon"></i></div>
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| − | </div>
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| − | <div class="spacer"></div>
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| − | </div>
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| − | </div>
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| − | <div class="ui vertical stripe segment"> | + | <div class="ui text container"> |
| − | <div class="ui middle aligned stackable grid container"> | + | <h1 class="ui teal header"> |
| − | <div class="row"> | + | UFSCar-Brasil |
| − | <div class="eight wide column">
| + | </h1> |
| − | <h3 class="ui header">The Project</h3>
| + | <h2 class="ui teal header">People helping people</h2> |
| − | <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Aliquam voluptate nihil nesciunt ut minus atque eaque voluptates provident laboriosam explicabo quia eligendi quam vero esse quos vel, mollitia nobis eveniet.</p>
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| − | <h3 class="ui header">Something</h3>
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| − | <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Deleniti temporibus ullam minus hic labore quaerat, nisi optio, earum illo sapiente assumenda molestias minima voluptatibus. Saepe laudantium soluta error veniam alias.</p>
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| − | </div>
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| − | <div class="six wide right floated column">
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| − | <img src="http://placehold.it/500x500" class="ui large bordered rounded image">
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| − | </div>
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| − | </div>
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| − | <div class="row">
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| − | <div class="center aligned column">
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| − | <a class="ui huge button">Check our project!</a>
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| − | </div>
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| − | </div>
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| − | </div>
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| | </div> | | </div> |
| | + | <div class="spacer"></div> |
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| − | <div class="ui vertical stripe segment "> | + | <div class="ui vertical stripe segment stackable two column grid container"> |
| − | <div class="ui middle aligned stackable grid container">
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| − | | + | <h3 class="ui header" id="overview">Bug Shoo - an innovative repellent</h3> |
| − | <div class="six wide left floated column">
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| − | <img src="http://placehold.it/500x500" class="ui large bordered rounded image">
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| − | </div>
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| | | | |
| − | <div class="eight wide column">
| + | <p> Our team designed a product, manufactured with tools of synthetic biology, which should be effective in combating diseases transmitted by insect vectors, such as dengue fever, malaria, yellow fever and leishmaniasis. Its active ingredient will be |
| − | <h3 class="ui header">The Team</h3>
| + | D-limonene, a terpene naturally produced by citrus plants, which has a repellent effect. Its production will be performed by a specific genic circuit triggered by plasmolysis. It will ensure its functioning just in human skin. After all, bacteria |
| − | <p> Lorem ipsum dolor sit amet, consectetur adipisicing elit. Aliquam voluptate nihil nesciunt ut minus atque eaque voluptates provident laboriosam explicabo quia eligendi quam vero esse quos vel, mollitia nobis eveniet. </p> | + | will dye by an innovative kill switch system. Find out in our project the main characteristics that make Bug Shoo a single repellent which would be presented later to the market. Also, follow along as our team has developed Policy & Practices of |
| − | <h3 class="ui header">Something</h3>
| + | structured activities to raise awareness about dengue fever prevention and Synthetic Biology issues.</p> |
| − | <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Deleniti temporibus ullam minus hic labore quaerat, nisi optio, earum illo sapiente assumenda molestias minima voluptatibus. Saepe laudantium soluta error veniam alias.</p>
| + | </p> |
| − | </div>
| + | </div> |
| − | </div>
| + | |
| − | <div class="row">
| + | |
| − | <div class="center aligned column">
| + | |
| − | <a class="ui huge button">Check our team!</a>
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| − | </div>
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| − | </div>
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| − | </div>
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| − | </div>
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| − | <div class=" ui vertical stripe segment"> | + | |
| − | <div class="ui text container">
| + | <img class="ui large rounded image" src="https://static.igem.org/mediawiki/2015/9/99/UFSCar-Brasil_bug_shoo.png"> |
| − | <h3 class="ui header">Breaking The Grid, Grabs Your Attention</h3>
| + | </div> |
| − | <p>Instead of focusing on content creation and hard work, we have learned how to master the art of doing nothing by providing massive amounts of whitespace and generic content that can seem massive, monolithic and worth your attention.</p>
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| − | <a class="ui large button">Read More</a>
| + | |
| − | <h4 class="ui horizontal header divider">
| + | |
| − | <a href="#">Case Studies</a>
| + | |
| − | </h4>
| + | |
| − | <h3 class="ui header">Lorem ipsum?</h3>
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| − | <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Iusto aperiam ratione officiis ex error deleniti, numquam odio blanditiis vero similique nam reprehenderit quis enim culpa deserunt iure. Quas, harum, esse.</p>
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| − | <a class="ui large button">I'm Still Quite Interested</a>
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| − | </div>
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| − | </div>
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| − | <div class=" ui vertical stripe segment"> | + | <div class=" centered row"> |
| − | <div class=" ui container"> | + | |
| − | <h3 class="ui center aligned header">Our amazing sponsors!</h3>
| + | <h3 class="ui center aligned teal header">See more!</h3> |
| − | <div class=" sponsor slider"> | + | <div class=" ui two huge buttons"> |
| − | <img src="img/logo/aquarela.jpg" alt="">
| + | <a href="https://2015.igem.org/Team:UFSCar-Brasil/Description" class="ui teal button">Our project</a> |
| − | <img src="img/logo/ccbs.gif" alt="">
| + | <div class="or"></div> |
| − | <img src="img/logo/exxtend.png" alt="">
| + | <a href="https://2015.igem.org/wiki/index.php?title=Team:UFSCar-Brasil/team.html" class="ui teal button">Our team</a> |
| − | <img src="img/logo/idt.jpg" alt="">
| + | |
| − | <img src="img/logo/igem.png" alt="">
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| − | <img src="img/logo/lmp.jpg" alt="">
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| − | <img src="img/logo/mathworks.jpg" alt="">
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| − | <img src="img/logo/ufscar.jpg" alt="">
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| − | <img src="img/logo/dge.png" alt="">
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| − | </div>
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| − | <div class="ufscar slider"> | + | |
| − | <img src="img/logo/dmp.jpg" alt="">
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| − | <img src="img/logo/propg.jpg" alt="">
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| − | <img src="img/logo/propq.jpg" alt="">
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| − | </div>
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| − | </div>
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| − | </div>
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| − | <div class="ui inverted vertical footer segment">
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| − | <div class="ui container">
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| − | <div class="ui stackable inverted divided equal height stackable grid"> | + | |
| − | <div class="three wide column">
| + | |
| − | <h4 class="ui inverted header">About</h4>
| + | |
| − | <div class="ui inverted link list">
| + | |
| − | <a href=" #" class="item">Sitemap</ a> | + | |
| − | <a href="#" class="item">Contact Us</a>
| + | |
| − | <a href="#" class="item">Religious Ceremonies</a>
| + | |
| − | <a href="#" class="item">Gazebo Plans</a>
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| − | </div>
| + | |
| − | </div>
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| − | <div class="three wide column">
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| − | <h4 class="ui inverted header">Services</h4>
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| − | <div class="ui inverted link list">
| + | |
| − | <a href="#" class="item">Pre-Order</a>
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| − | <a href="#" class="item">DNA FAQ</a>
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| − | <a href="#" class="item">How To Access</a>
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| − | <a href="#" class="item">Favorite</a>
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| − | </div>
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| − | </div>
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| − | <div class="seven wide column">
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| − | <h4 class="ui inverted header">Footer Header</h4>
| + | |
| − | <p>Extra space for a call to action inside the footer that could help re-engage users.</p>
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| − | </div>
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| − | </div>
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| | </div> | | </div> |
| | </div> | | </div> |
| | </div> | | </div> |
| − | <h2> Project Description </h2>
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| − | <p align="justify">Diseases transmitted by insect vectors, such as malaria and dengue, affect significantly the Brazilian population. In our city, São Carlos, this year in summer the public health organ featured a big number of cases. Working in this problem, our project consists in the development of an alternative repellent of that currently sold in the market and more or equally effective in preventing mosquitoes bites transmitted diseases. The main compound in current repellents is DEET (N, N-diethyl-m-toluamide), a toxic molecule which at certain concentrations could be lethal, and therefore must have strict control on their use in products. The main characteristic of our repellent is the long duration when compared to other products and the replacement of the compound DEET by D-limonene. Instead, the D-limonene has low toxicity, is highly volatile and present a pleasant smell.</p>
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| − | <p align="justify">Our proposal is building a bacteria carrying out the production of D-limonene via limonene synthase. To enable long term storage at room temperatures, the bacterial cells that make up the repellent will be plasmolyzed, with suspended metabolism, considered a dormant state. The maintenance of this state will be obtained by a solution of polyethyleneglycol (PEG) that will raise the osmotic pressures. Once in contact with the skin, the PEG solution will be diluted by sweat, inducing an osmotic shock in cells. Then the universal stress protein promoter (UspA) will be activated and will induce the expression of limonene synthase. Beyond PEG, other compounds such as glycerol and metal ions in low concentrations will compose our insect repellent cream, in order to sustain the bacteria to the required metabolites building and posterior enzyme activities. In this way, the distribution of our repellent may become feasible, allowing its use.</p>
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| − | <p align="justify">To overcome those problems with limonene synthase folding, we used constitutive promoters for the expression of chaperones from all Escherichia coli available classes (like ClpB, DnaK and IbpA / IbpB). It will reinforce the stockpile of chaperones naturally produced during osmotic shocks (heat-shock proteins) in bacteria. Our goal is to improve protein solubility, indirectly creatinga toolkit for protein solubility enhancement for future iGEM teams. . Besides, reducing the occurrence of insoluble bodies and improving the production of limonene.</p>
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| − | <p align="justify">Finally, a way to ensure the biosafety in the use of our repellent and keep a control of bacterial cells is the use of a system of programmed death or Kill Switch. This system will work with two suicide genes: Killer red, a protein which trigger oxidative species generation destroying the DNA (plasmid and chromosomal) allied to a potent RNAse which will avoid any contribution of RNA to host or normal microbiota. These genes will be switch on through an association with a zinc sensitive promoter triggered by zur proteins which associate with zinc and avoid the expression of them allowing the bacterial growing. After long periods of repellent action, bacteria activities will decrease zinc concentration in medium, which allows the gene activation and cells death.</p>
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