Difference between revisions of "Team:UFSCar-Brasil"

 
(93 intermediate revisions by 5 users not shown)
Line 1: Line 1:
−
{{UFSCar-Brasil}}
+
{{:Team:UFSCar-Brasil/Templates/Head}}
 +
 
 
<html>
 
<html>
−
<h2> Project Description </h2>
+
<style>
−
<p>Diseases transmitted by insect vectors, such as malaria and dengue, affect significantly the Brazilian population. In our city, San Carlos, this year in summer the public health organ featured a big number of cases. Working in this problem, our project consists in the development of an alternative repellent of that currently sold in the market and more or equally effective in preventing mosquitoes bites transmitted diseases. The main compound in current repellents isDEET (N, N-diethyl-m-toluamide), a toxic molecule which at certain concentrations could be lethal, and therefore must have strict control on their use in products. The main characteristic of our repellent is the long duration when compared to other products and the replacement of the compound DEET  by D-limonene. Instead, the D-limonene has low toxicity, is highly volatile and present a pleasant smell.
+
  .sponsor img,
−
Our proposal is building a bacteria carrying out the production of D-limonene via limonene synthase. To enable long term storage at room temperatures, the bacterial cells that make up the repellent will be plasmolyzed, with suspended metabolism, considered a dormant state. The maintenance of this state will be obtained by a solution of polyethyleneglycol (PEG) that will raise the osmotic pressures. Once in contact with the skin, the PEG solution will be diluted by sweat, inducing an osmotic shock in cells. Then the universal stress protein promoter (UspA) will be activated and will induce the expression of limonene synthase. Beyond PEG, other compounds such as glycerol and metal ions in low concentrations will compose our insect repellent cream, in order to sustain the bacteria to the required metabolites building and posterior enzyme activities. In this way, the distribution of our repellent may become feasible, allowing its use.</p>
+
  .ufscar img {
−
<p>To overcome those problems with limonene synthase folding, we used constitutive promoters for the expression of chaperones from all Escherichia coli available classes (like ClpB, DnaK and IbpA / IbpB). It will reinforce the stockpile of chaperones naturally produced during osmotic shocks (heat-shock proteins) in bacteria. Our goal is to improve protein solubility, indirectly creatinga toolkit for protein solubility enhancement for future iGEM teams. . Besides, reducing the occurrence of insoluble bodies and improving the production of limonene.</p>
+
    height: 150px;
−
<p>Finally, a way to ensure the biosafety in the use of our repellent and keep a control of bacterial cells is the use of a system of programmed death or Kill Switch. This system will work with two suicide genes: Killer red, a protein which trigger oxidative species generation destroying the DNA (plasmid and chromosomal) allied to a potent RNAse which will avoid any contribution of RNA to host or normal microbiota. These genes will be switch on through an association with a zinc sensitive promoter triggered by zur proteins which  associate with zinc and avoid the expression of them allowing the bacterial growing. After long periods of repellent action, bacteria activities will decrease zinc concentration in medium, which allows the gene activation and cells death.</p>
+
  }
−
</div></div> <!--These are the closing tags for div id="mainContainer" and div id="contentContainer". The corresponding opening tags appear in the template that is {{included}} at the top of this page.-->
+
 
 +
  h1.ui.header {
 +
    padding: 0 0;
 +
    margin-bottom: 0;
 +
  }
 +
 
 +
  h2.ui.header {
 +
    padding: 0 0;
 +
    margin: 0 0;
 +
  }
 +
 
 +
  .segment .text.container {
 +
    padding: 0 0;
 +
  }
 +
 
 +
  h1.firstHeading {
 +
    display: none;
 +
  }
 +
</style>
 +
 
 +
<div class="ui hidden divider"></div>
 +
 
 +
<div class="ui vertical center aligned inverted segment">
 +
  <div class="ui fluid container">
 +
 
 +
    <img class="ui fluid image" src="https://static.igem.org/mediawiki/2015/e/e1/Banner-1.jpg" alt="">
 +
 
 +
 
 +
    <div class="ui hidden divider"></div>
 +
 
 +
    <div class="ui text container">
 +
      <h1 class="ui teal header">
 +
        UFSCar-Brasil
 +
      </h1>
 +
      <h2 class="ui teal header">People helping people</h2>
 +
    </div>
 +
    <div class="spacer"></div>
 +
  </div>
 +
</div>
 +
 
 +
<div class="ui vertical stripe segment stackable two column grid container">
 +
  <div class="column">
 +
    <h3 class="ui header" id="overview">Bug Shoo - an innovative repellent</h3>
 +
 
 +
    <p>Our team designed a product, manufactured with tools of synthetic biology, which should be effective in combating diseases transmitted by insect vectors, such as dengue fever, malaria, yellow fever and leishmaniasis. Its active ingredient will be
 +
      D-limonene, a terpene naturally produced by citrus plants, which has a repellent effect. Its production will be performed by a specific genic circuit triggered by plasmolysis. It will ensure its functioning just in human skin. After all, bacteria
 +
      will dye by an innovative kill switch system. Find out in our project the main characteristics that make Bug Shoo a single repellent which would be presented later to the market. Also, follow along as our team has developed Policy & Practices of
 +
      structured activities to raise awareness about dengue fever prevention and Synthetic Biology issues.</p>
 +
    </p>
 +
  </div>
 +
 
 +
  <div class="column">
 +
    <img class="ui large rounded image" src="https://static.igem.org/mediawiki/2015/9/99/UFSCar-Brasil_bug_shoo.png">
 +
  </div>
 +
 
 +
  <div class="centered row">
 +
    <div class="column">
 +
      <h3 class="ui center aligned teal header">See more!</h3>
 +
      <div class="ui two huge buttons">
 +
        <a href="https://2015.igem.org/Team:UFSCar-Brasil/Description" class="ui teal button">Our project</a>
 +
        <div class="or"></div>
 +
        <a href="https://2015.igem.org/wiki/index.php?title=Team:UFSCar-Brasil/team.html" class="ui teal button">Our team</a>
 +
      </div>
 +
    </div>
 +
  </div>
 +
</div>
  
 
</html>
 
</html>
 +
 +
{{:Team:UFSCar-Brasil/Templates/Footer}}

Latest revision as of 19:10, 18 September 2015

Home
Project
Overview
Issue Plasmolysis and Limonene synthase Protein Solubilization Toolkit Kill Switch
Team
Our Team Attributions
Achievements
Overview
Parts Results Judging Criteria
Policy & Practices
Overview
Political Social Educational Safety/Security Meetings Fundraising
Modeling
Overview
Plasmolysis Protein Solubilization Toolkit Kill Switch
Notebook
Protocols Problems Collaborations Safety Acknowledgements

UFSCar-Brasil

People helping people

Bug Shoo - an innovative repellent

Our team designed a product, manufactured with tools of synthetic biology, which should be effective in combating diseases transmitted by insect vectors, such as dengue fever, malaria, yellow fever and leishmaniasis. Its active ingredient will be D-limonene, a terpene naturally produced by citrus plants, which has a repellent effect. Its production will be performed by a specific genic circuit triggered by plasmolysis. It will ensure its functioning just in human skin. After all, bacteria will dye by an innovative kill switch system. Find out in our project the main characteristics that make Bug Shoo a single repellent which would be presented later to the market. Also, follow along as our team has developed Policy & Practices of structured activities to raise awareness about dengue fever prevention and Synthetic Biology issues.

See more!

Our project
Our team

Our amazing sponsors!

Links

iGEM UFSCar

About

Team

Thanks for reading our wiki!

Come Again :)