Difference between revisions of "Team:EPF Lausanne/Part Collection"

 
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<div class="col-md-6">
 
<div class="col-md-6">
 
<h2>dCas9-ω</h2>
 
<h2>dCas9-ω</h2>
−
<p>dCa9s-ω is our main tool to build our project in <i>E. coli</i>. This catalytically dead Cas9 fused to the RNA polymerase ω subunit can act as an activator or as an inhibitor when provided the right guide RNA.</p>
+
<p>dCa9s-ω is our main tool to build our project in <i>E. coli</i>. This catalytically dead Cas9 fused to the RNA polymerase ω subunit can act as an activating or inhibiting transcription factor,  when provided with the right guide RNA.</p>
 
</div>
 
</div>
  
Line 48: Line 48:
 
<div class="col-md-6">
 
<div class="col-md-6">
 
<h2>PAM rich URS J23117 promoter</h2>
 
<h2>PAM rich URS J23117 promoter</h2>
−
<p>This promoter is composed of the weak J23117 promoter, BBa_J23117, fused to a PAM rich upstream regulatory region (URS) that is needed for dCas9 to bind as dCas9 cannot bind without a PAM sequence.</p>
+
<p>This promoter is composed of the weak J23117 promoter, BBa_J23117, fused to a PAM rich Upstream Regulatory Sequence (URS). Remember that dCas9 can bind only to PAM-flanked target sites.</p>
 
</div>
 
</div>
  
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<div class="col-md-6">
 
<div class="col-md-6">
 
<h2>sgRNA Z0 expressing cassette</h2>
 
<h2>sgRNA Z0 expressing cassette</h2>
−
<p>the sgRNA (single guide-RNA) Z0 inhibit PAM rich URS J23117 promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence Z0 makes this part unique, and the scaffold is a common part to all the sgRNAs and is recognized by dCas9-ω.</p>
+
<p>sgRNA (single guide-RNA) Z0 inhibits PAM rich URS J23117 promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence Z0 makes this part unique, and the scaffold is a common part to all sgRNAs. </p>
 
</div>
 
</div>
  
Line 84: Line 84:
 
<div class="col-md-6">
 
<div class="col-md-6">
 
<h2>sgRNA Z4 expressing cassette</h2>
 
<h2>sgRNA Z4 expressing cassette</h2>
−
<p>the sgRNA Z4 activates PAM rich URS J23117 promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence Z4 makes this part unique, and the scaffold is a common part to all the sgRNAs and is recognized by dCas9-ω.</p>
+
<p>sgRNA Z4 activates PAM rich URS J23117 promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence Z4 makes this part unique, and the scaffold is a common part to all the sgRNAs.</p>
−
<p><font color="red">This part is not RFC10. Removing bad restriction site would imply a lose of function.</font></p>
+
<p><font color="red">This part is not RFC10 compatible. Removing bad restriction site would imply a lose of function.</font></p>
 
</div>
 
</div>
  
Line 103: Line 103:
 
<div class="col-md-6">
 
<div class="col-md-6">
 
<h2>sgRNA Z35 expressing cassette</h2>
 
<h2>sgRNA Z35 expressing cassette</h2>
−
<p>the sgRNA Z35 inhibit PAM rich URS J23117 promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence Z35 makes this part unique, and the scaffold is a common part to all the sgRNAs and is recognized by dCas9-ω.</p>
+
<p>sgRNA Z35 inhibits PAM rich URS J23117 promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence Z35 makes this part unique, and the scaffold is a common part to all the sgRNAs.</p>
 
</div>
 
</div>
  
Line 120: Line 120:
 
<div class="col-md-6">
 
<div class="col-md-6">
 
<h2>PAM rich URS J23117Alt promoter</h2>
 
<h2>PAM rich URS J23117Alt promoter</h2>
−
<p>This promoter was made on the same template as PAM rich URS J23117 promoter, but was mutated to obtain different sequences for sgRNA targeting. It is also fused to a PAM rich upstream regulatory region (URS) that is needed for dCas9 to bind, as dCas9 cannot bind without a PAM sequence.</p>
+
<p>This promoter was made on the same template as PAM rich URS J23117 promoter, but was mutated to use different sgRNAs. It is also fused to a PAM rich upstream regulatory region (URS). Remember that dCas9 can bind only to PAM-flanked target sites.</p>
 
</div>
 
</div>
  
Line 138: Line 138:
 
<div class="col-md-6">
 
<div class="col-md-6">
 
<h2>sgRNA X0 expressing cassette</h2>
 
<h2>sgRNA X0 expressing cassette</h2>
−
<p>the sgRNA (single guide-RNA) X0 inhibit PAM rich URS J23117Alt promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence X0 makes this part unique, and the scaffold is a common part to all the sgRNAs and is recognized by dCas9-ω.</p>
+
<p>sgRNA (single guide-RNA) X0 inhibits PAM rich URS J23117Alt promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence X0 makes this part unique, and the scaffold is a common part to all the sgRNAs.</p>
 
</div>
 
</div>
  
Line 156: Line 156:
 
<div class="col-md-6">
 
<div class="col-md-6">
 
<h2>sgRNA X4 expressing cassette</h2>
 
<h2>sgRNA X4 expressing cassette</h2>
−
<p>the sgRNA X4 activates PAM rich URS J23117Alt promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence X4 makes this part unique, and the scaffold is a common part to all the sgRNAs and is recognized by dCas9-ω.</p>
+
<p>sgRNA X4 activates PAM rich URS J23117Alt promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence X4 makes this part unique, and the scaffold is a common part to all the sgRNAs.</p>
 
</div>
 
</div>
  
Line 174: Line 174:
 
<div class="col-md-6">
 
<div class="col-md-6">
 
<h2>sgRNA X35 expressing cassette</h2>
 
<h2>sgRNA X35 expressing cassette</h2>
−
<p>the sgRNA X35 activates PAM rich URS J23117Alt promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence X35 makes this part unique, and the scaffold is a common part to all the sgRNAs and is recognized by dCas9-ω.</p>
+
<p>sgRNA X35 activates PAM rich URS J23117Alt promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence X35 makes this part unique, and the scaffold is a common part to all the sgRNAs.</p>
 
</div>
 
</div>
  
Line 197: Line 197:
 
<h2>dCas9_VP64</h2>
 
<h2>dCas9_VP64</h2>
 
<p>dCa9s-VP64 is the main tool to build our project in <i>S. cerevisiae</i>. This catalytically dead Cas9 fused to a nuclear localization sequence (NLS) and the eukaryotic transcription factor VP64 can act as an activating (or as an inhibitin) transcription factor when forming a complex with the appropriate guide RNA.</p>
 
<p>dCa9s-VP64 is the main tool to build our project in <i>S. cerevisiae</i>. This catalytically dead Cas9 fused to a nuclear localization sequence (NLS) and the eukaryotic transcription factor VP64 can act as an activating (or as an inhibitin) transcription factor when forming a complex with the appropriate guide RNA.</p>
 +
<p><font color="red">This part is not RFC10 compatible.</font></p>
 
</div>
 
</div>
  
Line 212: Line 213:
 
<div class="col-md-6">
 
<div class="col-md-6">
 
<h2>c3_0 gRNA expressing sequence</h2>
 
<h2>c3_0 gRNA expressing sequence</h2>
−
<p>This sequence produces guide RNA c3_0 when put under the control of a RNA polymerase II promoter. gRNA c3_0 can form a complex together with dCas9_VP64 which activates promoter CYC_0.</p>
+
<p>This sequence produces guide RNA c3_0 when put under the control of a RNA polymerase II promoter. gRNA c3_0 forms a complex together with dCas9_VP64 which activates promoter CYC_0.</p>
 
</div>
 
</div>
  
Line 228: Line 229:
 
<div class="col-md-6">
 
<div class="col-md-6">
 
<h2>c3_1 gRNA expressing sequence</h2>
 
<h2>c3_1 gRNA expressing sequence</h2>
−
<p>This sequence produces guide RNA c3_1 when put under the control of a RNA polymerase II promoter. gRNA c3_0 can form a complex together with dCas9_VP64 which activates promoter CYC_1.</p>
+
<p>This sequence produces guide RNA c3_1 when put under the control of a RNA polymerase II promoter. gRNA c3_1 forms a complex together with dCas9_VP64 which activates promoter CYC_1.</p>
 
</div>
 
</div>
  
Line 244: Line 245:
 
<div class="col-md-6">
 
<div class="col-md-6">
 
<h2>c3_2 gRNA expressing sequence</h2>
 
<h2>c3_2 gRNA expressing sequence</h2>
−
<p>This sequence produces guide RNA c3_2 when put under the control of a RNA polymerase II promoter. gRNA c3_0 can form a complex together with dCas9_VP64 which activates promoter CYC_2.</p>
+
<p>This sequence produces guide RNA c3_2 when put under the control of a RNA polymerase II promoter. gRNA c3_2 forms a complex together with dCas9_VP64 which activates promoter CYC_2.</p>
 
</div>
 
</div>
  
Line 260: Line 261:
 
<div class="col-md-6">
 
<div class="col-md-6">
 
<h2>c3_3 gRNA expressing sequencee</h2>
 
<h2>c3_3 gRNA expressing sequencee</h2>
−
<p>This sequence produces guide RNA c3_3 when put under the control of a RNA polymerase II promoter. gRNA c3_0 can form a complex together with dCas9_VP64 which activates promoter CYC_3.</p>
+
<p>This sequence produces guide RNA c3_3 when put under the control of a RNA polymerase II promoter. gRNA c3_3 forms a complex together with dCas9_VP64 which activates promoter CYC_3.</p>
 
</div>
 
</div>
  
Line 276: Line 277:
 
<div class="col-md-6">
 
<div class="col-md-6">
 
<h2>c6_0 gRNA expressing sequence</h2>
 
<h2>c6_0 gRNA expressing sequence</h2>
−
<p>This sequence produces guide RNA c6_0 when put under the control of a RNA polymerase II promoter. gRNA c6_0 can form a complex together with dCas9_VP64 which inhibits promoter CYC_0.</p>
+
<p>This sequence produces guide RNA c6_0 when put under the control of a RNA polymerase II promoter. gRNA c6_0 forms a complex together with dCas9_VP64 which inhibits promoter CYC_0.</p>
 
</div>
 
</div>
  
Line 292: Line 293:
 
<div class="col-md-6">
 
<div class="col-md-6">
 
<h2>c6_1 gRNA expressing sequence</h2>
 
<h2>c6_1 gRNA expressing sequence</h2>
−
<p>This sequence produces guide RNA c6_1 when put under the control of a RNA polymerase II promoter. gRNA c6_1 can form a complex together with dCas9_VP64 which inhibits promoter CYC_1.</p>
+
<p>This sequence produces guide RNA c6_1 when put under the control of a RNA polymerase II promoter. gRNA c6_1 forms a complex together with dCas9_VP64 which inhibits promoter CYC_1.</p>
 
</div>
 
</div>
  
Line 308: Line 309:
 
<div class="col-md-6">
 
<div class="col-md-6">
 
<h2>c6_2 gRNA expressing sequence</h2>
 
<h2>c6_2 gRNA expressing sequence</h2>
−
<p>This sequence produces guide RNA c6_2 when put under the control of a RNA polymerase II promoter. gRNA c6_2 can form a complex together with dCas9_VP64 which inhibits promoter CYC_2.</p>
+
<p>This sequence produces guide RNA c6_2 when put under the control of a RNA polymerase II promoter. gRNA c6_2 forms a complex together with dCas9_VP64 which inhibits promoter CYC_2.</p>
 
</div>
 
</div>
  
Line 324: Line 325:
 
<div class="col-md-6">
 
<div class="col-md-6">
 
<h2>c6_3 gRNA expressing sequence</h2>
 
<h2>c6_3 gRNA expressing sequence</h2>
−
<p>This sequence produces guide RNA c6_3 when put under the control of a RNA polymerase II promoter. gRNA c6_3 can form a complex together with dCas9_VP64 which inhibits promoter CYC_3.</p>
+
<p>This sequence produces guide RNA c6_3 when put under the control of a RNA polymerase II promoter. gRNA c6_3 forms a complex together with dCas9_VP64 which inhibits promoter CYC_3.</p>
 
</div>
 
</div>
  
Line 340: Line 341:
 
<div class="col-md-6">
 
<div class="col-md-6">
 
<h2>c7_0 gRNA expressing sequence</h2>
 
<h2>c7_0 gRNA expressing sequence</h2>
−
<p>This sequence produces guide RNA c7_0 when put under the control of a RNA polymerase II promoter. gRNA c7_0 can form a complex together with dCas9_VP64 which inhibits promoter CYC_0.</p>
+
<p>This sequence produces guide RNA c7_0 when put under the control of a RNA polymerase II promoter. gRNA c7_0 forms a complex together with dCas9_VP64 which inhibits promoter CYC_0.</p>
 
</div>
 
</div>
  
Line 356: Line 357:
 
<div class="col-md-6">
 
<div class="col-md-6">
 
<h2>c7_1 gRNA expressing sequence</h2>
 
<h2>c7_1 gRNA expressing sequence</h2>
−
<p>This sequence produces guide RNA c7_1 when put under the control of a RNA polymerase II promoter. gRNA c7_1 can form a complex together with dCas9_VP64 which inhibits promoter CYC_1.</p>
+
<p>This sequence produces guide RNA c7_1 when put under the control of a RNA polymerase II promoter. gRNA c7_1 forms a complex together with dCas9_VP64 which inhibits promoter CYC_1.</p>
 
</div>
 
</div>
  
 
<div class="col-md-3 text-center">
 
<div class="col-md-3 text-center">
 
<br><br><br><br><br>
 
<br><br><br><br><br>
−
<a href="http://parts.igem.org/Part:BBa_K1723018"><b>BBa_K1723018"</b></a>
+
<a href="http://parts.igem.org/Part:BBa_K1723018"><b>BBa_K1723018</b></a>
 
</div>
 
</div>
 
</div>
 
</div>
Line 372: Line 373:
 
<div class="col-md-6">
 
<div class="col-md-6">
 
<h2>c7_2 gRNA expressing sequence</h2>
 
<h2>c7_2 gRNA expressing sequence</h2>
−
<p>This sequence produces guide RNA c7_2 when put under the control of a RNA polymerase II promoter. gRNA c7_2 can form a complex together with dCas9_VP64 which inhibits promoter CYC_2.</p>
+
<p>This sequence produces guide RNA c7_2 when put under the control of a RNA polymerase II promoter. gRNA c7_2 forms a complex together with dCas9_VP64 which inhibits promoter CYC_2.</p>
 
</div>
 
</div>
  
Line 387: Line 388:
  
 
<div class="col-md-6">
 
<div class="col-md-6">
−
<h2>c7_3 gRNA expressing sequence</p>
+
<h2>c7_3 gRNA expressing sequence</h2>
−
<p>This sequence produces guide RNA c7_3 when put under the control of a RNA polymerase II promoter. gRNA c7_3 can form a complex together with dCas9_VP64 which inhibits promoter CYC_3.</p>
+
<p>This sequence produces guide RNA c7_3 when put under the control of a RNA polymerase II promoter. gRNA c7_3 forms a complex together with dCas9_VP64 which inhibits promoter CYC_3.</p>
 
</div>
 
</div>
  
Line 394: Line 395:
 
<br><br><br><br><br>
 
<br><br><br><br><br>
 
<a href="http://parts.igem.org/Part:BBa_K1723020"><b>BBa_K1723020</b></a>
 
<a href="http://parts.igem.org/Part:BBa_K1723020"><b>BBa_K1723020</b></a>
 +
</div>
 +
 +
</div>
 +
<div class="row">
 +
<div class="col-md-3 text-center">
 +
<img src="https://static.igem.org/mediawiki/2015/b/b6/EPF_Lausanne_CYC_0.png" style="width:75%">
 +
</div>
 +
 +
<div class="col-md-6">
 +
<h2>CYC_0 promoter</h2>
 +
<p>CYC_0 promoter acts as a transistor in yeast. It is activated by the complex c3_0-dCas9_VP64 but repressed by complexes c6_0-dCas9_VP64 and c7_0-dCas9_VP64. When any combination of those three complexes is expressed, inhibition prevails.</p>
 +
</div>
 +
 +
<div class="col-md-3 text-center">
 +
<br><br><br><br><br>
 +
<a href="http://parts.igem.org/Part:BBa_K1723022"><b>BBa_K1723022</b></a>
 +
</div>
 +
 +
</div>
 +
<div class="row">
 +
<div class="col-md-3 text-center">
 +
<img src="https://static.igem.org/mediawiki/2015/c/cb/EPF_Lausanne_CYC_1.png" style="width:75%">
 +
</div>
 +
 +
<div class="col-md-6">
 +
<h2>CYC_1 promoter</h2>
 +
<p>CYC_1 promoter acts as a transistor in yeast. It is activated by the complex c3_1-dCas9_VP64 but repressed by complexes c6_1-dCas9_VP64 and c7_1-dCas9_VP64. When any combination of those three complexes is expressed, inhibition prevails.</p>
 +
</div>
 +
 +
<div class="col-md-3 text-center">
 +
<br><br><br><br><br>
 +
<a href="http://parts.igem.org/Part:BBa_K1723023"><b>BBa_K1723023</b></a>
 +
</div>
 +
 +
</div>
 +
<div class="row">
 +
<div class="col-md-3 text-center">
 +
<img src="https://static.igem.org/mediawiki/2015/0/0f/EPF_Lausanne_CYC_2.png" style="width:75%">
 +
</div>
 +
 +
<div class="col-md-6">
 +
<h2>CYC_2 promoter</h2>
 +
<p>CYC_2 promoter acts as a transistor in yeast. It is activated by the complex c3_2-dCas9_VP64 but repressed by complexes c6_2-dCas9_VP64 and c7_2-dCas9_VP64. When any combination of those three complexes is expressed, inhibition prevails.</p>
 +
</div>
 +
 +
<div class="col-md-3 text-center">
 +
<br><br><br><br><br>
 +
<a href="http://parts.igem.org/Part:BBa_K1723024"><b>BBa_K1723024</b></a>
 +
</div>
 +
 +
</div>
 +
<div class="row">
 +
<div class="col-md-3 text-center">
 +
<img src="https://static.igem.org/mediawiki/2015/e/e2/EPF_Lausanne_CYC_3.png" style="width:75%">
 +
</div>
 +
 +
<div class="col-md-6">
 +
<h2>CYC_3 promoter</h2>
 +
<p>CYC_3 promoter acts as a transistor in yeast. It is activated by the complex c3_3-dCas9_VP64 but repressed by complexes c6_3-dCas9_VP64 and c7_3-dCas9_VP64. When any combination of those three complexes is expressed, inhibition prevails.</p>
 +
</div>
 +
 +
<div class="col-md-3 text-center">
 +
<br><br><br><br><br>
 +
<a href="http://parts.igem.org/Part:BBa_K1723025"><b>BBa_K1723025</b></a>
 
</div>
 
</div>
  

Latest revision as of 19:45, 18 September 2015

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

OUR PART COLLECTION

E. coli

dCas9-ω

dCa9s-ω is our main tool to build our project in E. coli. This catalytically dead Cas9 fused to the RNA polymerase ω subunit can act as an activating or inhibiting transcription factor, when provided with the right guide RNA.






BBa_K1723000

PAM rich URS J23117 promoter

This promoter is composed of the weak J23117 promoter, BBa_J23117, fused to a PAM rich Upstream Regulatory Sequence (URS). Remember that dCas9 can bind only to PAM-flanked target sites.






BBa_K1723001

sgRNA Z0 expressing cassette

sgRNA (single guide-RNA) Z0 inhibits PAM rich URS J23117 promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence Z0 makes this part unique, and the scaffold is a common part to all sgRNAs.






BBa_1723002

sgRNA Z4 expressing cassette

sgRNA Z4 activates PAM rich URS J23117 promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence Z4 makes this part unique, and the scaffold is a common part to all the sgRNAs.

This part is not RFC10 compatible. Removing bad restriction site would imply a lose of function.






BBa_1723003

sgRNA Z35 expressing cassette

sgRNA Z35 inhibits PAM rich URS J23117 promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence Z35 makes this part unique, and the scaffold is a common part to all the sgRNAs.






BBa_K1723004

PAM rich URS J23117Alt promoter

This promoter was made on the same template as PAM rich URS J23117 promoter, but was mutated to use different sgRNAs. It is also fused to a PAM rich upstream regulatory region (URS). Remember that dCas9 can bind only to PAM-flanked target sites.






BBa_K1723005

sgRNA X0 expressing cassette

sgRNA (single guide-RNA) X0 inhibits PAM rich URS J23117Alt promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence X0 makes this part unique, and the scaffold is a common part to all the sgRNAs.






BBa_K1723006

sgRNA X4 expressing cassette

sgRNA X4 activates PAM rich URS J23117Alt promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence X4 makes this part unique, and the scaffold is a common part to all the sgRNAs.






BBa_K1723007

sgRNA X35 expressing cassette

sgRNA X35 activates PAM rich URS J23117Alt promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence X35 makes this part unique, and the scaffold is a common part to all the sgRNAs.






BBa_K1723008

S. cerevisiae

dCas9_VP64

dCa9s-VP64 is the main tool to build our project in S. cerevisiae. This catalytically dead Cas9 fused to a nuclear localization sequence (NLS) and the eukaryotic transcription factor VP64 can act as an activating (or as an inhibitin) transcription factor when forming a complex with the appropriate guide RNA.

This part is not RFC10 compatible.






BBa_K1723021

c3_0 gRNA expressing sequence

This sequence produces guide RNA c3_0 when put under the control of a RNA polymerase II promoter. gRNA c3_0 forms a complex together with dCas9_VP64 which activates promoter CYC_0.






BBa_K1723009

c3_1 gRNA expressing sequence

This sequence produces guide RNA c3_1 when put under the control of a RNA polymerase II promoter. gRNA c3_1 forms a complex together with dCas9_VP64 which activates promoter CYC_1.






BBa_K1723010

c3_2 gRNA expressing sequence

This sequence produces guide RNA c3_2 when put under the control of a RNA polymerase II promoter. gRNA c3_2 forms a complex together with dCas9_VP64 which activates promoter CYC_2.






BBa_K1723011

c3_3 gRNA expressing sequencee

This sequence produces guide RNA c3_3 when put under the control of a RNA polymerase II promoter. gRNA c3_3 forms a complex together with dCas9_VP64 which activates promoter CYC_3.






BBa_K1723012

c6_0 gRNA expressing sequence

This sequence produces guide RNA c6_0 when put under the control of a RNA polymerase II promoter. gRNA c6_0 forms a complex together with dCas9_VP64 which inhibits promoter CYC_0.






BBa_K1723013

c6_1 gRNA expressing sequence

This sequence produces guide RNA c6_1 when put under the control of a RNA polymerase II promoter. gRNA c6_1 forms a complex together with dCas9_VP64 which inhibits promoter CYC_1.






BBa_K1723014

c6_2 gRNA expressing sequence

This sequence produces guide RNA c6_2 when put under the control of a RNA polymerase II promoter. gRNA c6_2 forms a complex together with dCas9_VP64 which inhibits promoter CYC_2.






BBa_K1723015

c6_3 gRNA expressing sequence

This sequence produces guide RNA c6_3 when put under the control of a RNA polymerase II promoter. gRNA c6_3 forms a complex together with dCas9_VP64 which inhibits promoter CYC_3.






BBa_K1723016

c7_0 gRNA expressing sequence

This sequence produces guide RNA c7_0 when put under the control of a RNA polymerase II promoter. gRNA c7_0 forms a complex together with dCas9_VP64 which inhibits promoter CYC_0.






BBa_K1723017

c7_1 gRNA expressing sequence

This sequence produces guide RNA c7_1 when put under the control of a RNA polymerase II promoter. gRNA c7_1 forms a complex together with dCas9_VP64 which inhibits promoter CYC_1.






BBa_K1723018

c7_2 gRNA expressing sequence

This sequence produces guide RNA c7_2 when put under the control of a RNA polymerase II promoter. gRNA c7_2 forms a complex together with dCas9_VP64 which inhibits promoter CYC_2.






BBa_K1723019

c7_3 gRNA expressing sequence

This sequence produces guide RNA c7_3 when put under the control of a RNA polymerase II promoter. gRNA c7_3 forms a complex together with dCas9_VP64 which inhibits promoter CYC_3.






BBa_K1723020

CYC_0 promoter

CYC_0 promoter acts as a transistor in yeast. It is activated by the complex c3_0-dCas9_VP64 but repressed by complexes c6_0-dCas9_VP64 and c7_0-dCas9_VP64. When any combination of those three complexes is expressed, inhibition prevails.






BBa_K1723022

CYC_1 promoter

CYC_1 promoter acts as a transistor in yeast. It is activated by the complex c3_1-dCas9_VP64 but repressed by complexes c6_1-dCas9_VP64 and c7_1-dCas9_VP64. When any combination of those three complexes is expressed, inhibition prevails.






BBa_K1723023

CYC_2 promoter

CYC_2 promoter acts as a transistor in yeast. It is activated by the complex c3_2-dCas9_VP64 but repressed by complexes c6_2-dCas9_VP64 and c7_2-dCas9_VP64. When any combination of those three complexes is expressed, inhibition prevails.






BBa_K1723024

CYC_3 promoter

CYC_3 promoter acts as a transistor in yeast. It is activated by the complex c3_3-dCas9_VP64 but repressed by complexes c6_3-dCas9_VP64 and c7_3-dCas9_VP64. When any combination of those three complexes is expressed, inhibition prevails.






BBa_K1723025
EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits