Difference between revisions of "Team:XJTLU-CHINA/Collaborations"

We provided plasmids synthesized by ourselves, E. coli strains and IPTG for NYU-Shanghai to support their experiment. Two kinds of functional plasmids were synthesized by us. One was the plasmid of FwYellow chromoprotein, noted for BBa _K1033910. The other was that of Aeblue chromoprotein, noted for BBa_K864401. We also supplied 5000μL of BL21 competent cell for them to create new type of E. coli. For inducing the expression of chromoproteins, we gave them IPTG as the inducer. All these biology materials were packaged hermetically and Victor Zhou sent them to teammates in NYU-Shanghai in person in order to prevent any separation during the transportation. They wanted to use arabinose to stimulate transcription but with no color, we suggested them to use different concentration of arabinose to find the best concentration to show the color. Because they used arabinose to stimulate transcription, we suggested that IPTG may be a better choice. At that time, we were using IPTG inducible plasmid pET21a respectively with blue chromoprotein and yellow chromoprotein in BL21 (DE3), both of them showed the color. Therefore, three weeks ago, they used our donation and made more samples. They thanked us a lot, and we were also proud of supporting other groups in 2015 iGEM competition.

Synbio Tech is an innovator in the field of synthetic biology in China, having a wide range of cooperation with both international companies and universities. It aims to provide customers with a perfect technical platform of the synthetic biology. For now, by applying their unique self-established technical platform Syno® involving sound chip-based gene synthesis and high throughput gene sequencing service, it already develops the technology of constructing recombinant antibody libraries as well as highly-purified vaccines, genome synthesis and DNA storage.
As a major sponsor of XJTLU-China 2015, they provided a lab equipped with all apparatuses and reagents needed and directly gave a hand in the de novo gene synthesis in the circuits’ construction. At the beginning of iGEM project, the company offered us a training program including both theoretical basis of synthetic biology and practical skills useful in later wet experiments. Also during the collaboration this summer, they were active in supervising our experiments and giving valuable suggestions on experiment protocols and result analysis.