Difference between revisions of "Team:FAU Erlangen/Tour34"
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− | < | + | <h1>Set backs </h1> |
− | rpd3 | + | <p class='intro'> |
+ | “Science never solves a problem without creating ten more” - <b>George Bernard Shaw</b><br> | ||
+ | </p> | ||
+ | <p> | ||
+ | <a class="popupImg alignLeft" style="width:350px" target="_blank" href="https://static.igem.org/mediawiki/2015/4/41/FAU_Setback2.jpeg" title="dt. gesperrt = engl. closed"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/4/41/FAU_Setback2.jpeg" style="width:350px" /> | ||
+ | </a> | ||
+ | </p> | ||
+ | <h4>Sequences</h4> | ||
+ | |||
+ | <ul> | ||
+ | |||
+ | <li> We planned to work with three different loci, yet forgot to synthesize a selectionmarker <b> within</b> | ||
+ | the sequence for the recombination. | ||
+ | |||
+ | <li>Due to misunderstanding the only working homologous insert for the can1 gene was not used. | ||
+ | </ul> | ||
+ | |||
+ | <h4>rpd3 </h4> | ||
<ul> | <ul> | ||
<li>the rpd3 clones sent for sequencing always contained mutations </li> | <li>the rpd3 clones sent for sequencing always contained mutations </li> | ||
</ul> | </ul> | ||
− | Golden Gate | + | <h4>Golden Gate </h4> |
<ul> | <ul> | ||
− | <li>we ordered a wrong enzyme with a way too high optimum --> reaction had very little production </li> | + | <li>we ordered a wrong enzyme with a way too high temperature optimum --> reaction had very little production </li> |
<li>accidentally digestion of our Golden Gate fragments over night with an enzyme with star activity </li> | <li>accidentally digestion of our Golden Gate fragments over night with an enzyme with star activity </li> | ||
</ul> | </ul> | ||
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Latest revision as of 20:43, 18 September 2015
Set backs
“Science never solves a problem without creating ten more” - George Bernard Shaw
Sequences
- We planned to work with three different loci, yet forgot to synthesize a selectionmarker within the sequence for the recombination.
- Due to misunderstanding the only working homologous insert for the can1 gene was not used.
rpd3
- the rpd3 clones sent for sequencing always contained mutations
Golden Gate
- we ordered a wrong enzyme with a way too high temperature optimum --> reaction had very little production
- accidentally digestion of our Golden Gate fragments over night with an enzyme with star activity