Difference between revisions of "Team:Technion HS Israel/Notebook"

Line 10: Line 10:
 
</head>
 
</head>
 
<rel="stylesheet">
 
<rel="stylesheet">
<div id="toc" >
+
<div id="toc" class="toc">
<ol><ol><ol><a href="#">Top</a>
+
<ol><ol><ol>
 +
<a href="#">Top</a>
 
<a href="#Summery">Summery</a>
 
<a href="#Summery">Summery</a>
 
<a href="#Preparation">Preparation</a>
 
<a href="#Preparation">Preparation</a>

Revision as of 21:57, 18 September 2015

Technion 2015 HS Team's Wiki

        Top Summery Preparation July 12th July 13th July 15th July 20th July 22th July 29th August 2th August 3th August 4th August 5th August 6th August 9th August 10th August 11th August 12th August 13th August 16th August 17th August 18th /div>

Notebook

Here is a summery of our notebook.

Week Design Cloning Experiment Finish new BioBrick
Jul 5th to Jul 9th (2015) Primers : OGEM 1 and 2 to open j09855 plasmid .

Gblock :RBS- Aiia with a Gibson overlabs.
we got j09855 part from the distribution kit. - -
June 12th to July 16th (2015) Primers : OGEM 3 and 4 to got the k1767001 plasmid without LuxR , after doing the PCR we will get a linear DNA so we put two enzymes in the overlaps of the primers to close the plasmids. PCR to open the j09855 plasmid.Gibson to add Aiia . - BBa_K1767001
Aug. 2nd to Aug. 6th (2015) - We got p0440 part from the distribution kit.

We built BBa_K1767002 and BBa_K1767004 Constructs by restriction enzymes.

PCR to get the k1767001 plasmid without LuxR .
- BBa_K1767002

BBa_K1767004
Aug. 9th to Aug. 13th (2015) Primers : OGEM 5 and 6 to check if we succeed in the PCR ( Those primers for colony PCR) We got I13521 part from the distribution kit.
We built BBa_K1767003 and BBa_K1767005 Constructs by restriction enzymes.
Colony PCR to check if we have LuxR in the plasmid.
- BBa_K1767003
BBa_K1767005
Aug. 16th to Aug. 20th (2015) - - AHL experiment #1 -
Aug. 23rd to Aug. 27th (2015) Primers : OGEM 7 and 8 to check if we have yfp in the plasmid ( for colony PCR ). We got I13003 part from the distribution kit. AHL experiment #2 -
Aug. 30th to Sept. 9th (2015) Primers: OGEM 9 and 10 to replaced the RFP with RFP ( with LVA ) We built BBa_K1767009 and BBa_K1767010 Constructs by restriction enzymes.
Colony PCR to check if we have YFP in the plasmid.
- BBa_K1767009
BBa_K1767010
Sept. 6th to Sept. 11th (2015) - - AHL experiment # 3 -

preparation

  • 1. LB medium
  • 2. Miniprep
Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5 https://2015.igem.org/File:Technion_HS_Israel_pre6 https://2015.igem.org/File:Technion_HS_Israel_pre7 https://2015.igem.org/File:Technion_HS_Israel_pre2 https://2015.igem.org/File:Technion_HS_Israel_pre3 https://2015.igem.org/File:Technion_HS_Israel_pre4

July 12th

Technion_HS_Israel_pre1

July 13th

Technion_HS_Israel_pre1

July 15th

Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5

July 20th

Technion_HS_Israel_pre1

July 22th

Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5

July 29th

Technion_HS_Israel_pre1

august 2th

Technion_HS_Israel_pre1

august 3th

Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5

august 4th

Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5

august 5th

Technion_HS_Israel_pre1

august 6th

Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5

august 9th

Technion_HS_Israel_pre1

august 10th

Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5

august 11th

Technion_HS_Israel_pre1

august 12th

Technion_HS_Israel_pre1

august 13th

Technion_HS_Israel_pre1

august 16th

Technion_HS_Israel_pre1

august 17th

Technion_HS_Israel_pre1

august 18th

Technion_HS_Israel_pre1

august 19th

Technion_HS_Israel_pre1

august 20th

Technion_HS_Israel_pre1

august 23th

Technion_HS_Israel_pre1

august 24th

Technion_HS_Israel_pre1

august 25th

Technion_HS_Israel_pre1

august 26th

Technion_HS_Israel_pre1

august 27th

Technion_HS_Israel_pre1

august 29th

Technion_HS_Israel_pre1

august 30th

Technion_HS_Israel_pre1

august 31th

Technion_HS_Israel_pre1

september 1th

Technion_HS_Israel_pre1

september 2th

Technion_HS_Israel_pre1

september 3th

Technion_HS_Israel_pre1

september 5th

Technion_HS_Israel_pre1

september 6th

Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5

september 7th

Technion_HS_Israel_pre1