Difference between revisions of "Team:Technion HS Israel/Notebook"

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<span>Contents</span>
 
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<li><a href="#"><span>Top</span></a></li>
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<li><a href="#Summery"><span>Summery</span></a></li>
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<li><a href="#Preparation"><span>Preparation</span></a></li>
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<li><a href="#July 12th"><span>July 12th</span></a></li>
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<li><a href="#July 13th"><span>July 13th</span></a></li>
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<li><a href="#July 15th"><span>July 15th</span></a></li>
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<li><a href="#July 20th"><span>July 20th</span></a></li>
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<li><a href="#July 22th"><span>July 22th</span></a></li>
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<li><a href="#July 29th"><span>July 29th</span></a></li>
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<li><a href="#august 2th"><span>August 2th</span></a></li>
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<li><a href="#august 3th"><span>August 3th</span></a></li>
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<li><a href="#august 4th"><span>August 4th</span></a></li>
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<li><a href="#august 5th"><span>August 5th</span></a></li>
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<li><a href="#august 6th"><span>August 6th</span></a></li>
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<li><a href="#august 9th"><span>August 9th</span></a></li>
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<li><a href="#august 10th"><span>August 10th</span></a></li>
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<li><a href="#august 11th"><span>August 11th</span></a></li>
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<li><a href="#august 12th"><span>August 12th</span></a></li>
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<li><a href="#august 13th"><span>August 13th</span></a></li>
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<li><a href="#august 16th"><span>August 16th</span></a></li>
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<li><a href="#august 17th"><span>August 17th</span></a></li>
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<li><a href="#august 18th"><span>August 18th</span></a></li>
 
<li><a href="#august 19th"><span>August 19th</span></a></li>
 
<li><a href="#august 19th"><span>August 19th</span></a></li>
 
<li><a href="#august 20th"><span>August 20th</span></a></li>
 
<li><a href="#august 20th"><span>August 20th</span></a></li>

Revision as of 22:17, 18 September 2015

Technion 2015 HS Team's Wiki

Notebook

Here is a summery of our notebook.

Week Design Cloning Experiment Finish new BioBrick
Jul 5th to Jul 9th (2015) Primers : OGEM 1 and 2 to open j09855 plasmid .

Gblock :RBS- Aiia with a Gibson overlabs.
we got j09855 part from the distribution kit. - -
June 12th to July 16th (2015) Primers : OGEM 3 and 4 to got the k1767001 plasmid without LuxR , after doing the PCR we will get a linear DNA so we put two enzymes in the overlaps of the primers to close the plasmids. PCR to open the j09855 plasmid.Gibson to add Aiia . - BBa_K1767001
Aug. 2nd to Aug. 6th (2015) - We got p0440 part from the distribution kit.

We built BBa_K1767002 and BBa_K1767004 Constructs by restriction enzymes.

PCR to get the k1767001 plasmid without LuxR .
- BBa_K1767002

BBa_K1767004
Aug. 9th to Aug. 13th (2015) Primers : OGEM 5 and 6 to check if we succeed in the PCR ( Those primers for colony PCR) We got I13521 part from the distribution kit.
We built BBa_K1767003 and BBa_K1767005 Constructs by restriction enzymes.
Colony PCR to check if we have LuxR in the plasmid.
- BBa_K1767003
BBa_K1767005
Aug. 16th to Aug. 20th (2015) - - AHL experiment #1 -
Aug. 23rd to Aug. 27th (2015) Primers : OGEM 7 and 8 to check if we have yfp in the plasmid ( for colony PCR ). We got I13003 part from the distribution kit. AHL experiment #2 -
Aug. 30th to Sept. 9th (2015) Primers: OGEM 9 and 10 to replaced the RFP with RFP ( with LVA ) We built BBa_K1767009 and BBa_K1767010 Constructs by restriction enzymes.
Colony PCR to check if we have YFP in the plasmid.
- BBa_K1767009
BBa_K1767010
Sept. 6th to Sept. 11th (2015) - - AHL experiment # 3 -

preparation

  • 1. LB medium
  • 2. Miniprep
Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5 https://2015.igem.org/File:Technion_HS_Israel_pre6 https://2015.igem.org/File:Technion_HS_Israel_pre7 https://2015.igem.org/File:Technion_HS_Israel_pre2 https://2015.igem.org/File:Technion_HS_Israel_pre3 https://2015.igem.org/File:Technion_HS_Israel_pre4

July 12th

Technion_HS_Israel_pre1

July 13th

Technion_HS_Israel_pre1

July 15th

Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5

July 20th

Technion_HS_Israel_pre1

July 22th

Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5

July 29th

Technion_HS_Israel_pre1

august 2th

Technion_HS_Israel_pre1

august 3th

Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5

august 4th

Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5

august 5th

Technion_HS_Israel_pre1

august 6th

Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5

august 9th

Technion_HS_Israel_pre1

august 10th

Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5

august 11th

Technion_HS_Israel_pre1

august 12th

Technion_HS_Israel_pre1

august 13th

Technion_HS_Israel_pre1

august 16th

Technion_HS_Israel_pre1

august 17th

Technion_HS_Israel_pre1

august 18th

Technion_HS_Israel_pre1

august 19th

Technion_HS_Israel_pre1

august 20th

Technion_HS_Israel_pre1

august 23th

Technion_HS_Israel_pre1

august 24th

Technion_HS_Israel_pre1

august 25th

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august 26th

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august 27th

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august 29th

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august 30th

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august 31th

Technion_HS_Israel_pre1

september 1th

Technion_HS_Israel_pre1

september 2th

Technion_HS_Israel_pre1

september 3th

Technion_HS_Israel_pre1

september 5th

Technion_HS_Israel_pre1

september 6th

Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5

september 7th

Technion_HS_Israel_pre1