Difference between revisions of "Team:Hong Kong-CUHK/Description"
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<p><font face="Times New Roman" size="8pt">Part Improvement:</font></p> | <p><font face="Times New Roman" size="8pt">Part Improvement:</font></p> | ||
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<p><font face="Times New Roman" size="5pt">Background:</font></p> | <p><font face="Times New Roman" size="5pt">Background:</font></p> | ||
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− | <p><font face="Times New Roman" size="4pt">Magnetosomes, an organelle having encapsulating magnetic iron crystal can be applied in many different aspects. One of the various applications is to construct a more efficient microbial fuel cell (MFC). MFC is a machine which uses electrons produced by microorganism to generate electricity. If we genetically modify the bacteria | + | <p><font face="Times New Roman" size="4pt">Magnetosomes, an organelle having encapsulating magnetic iron crystal can be applied in many different aspects. One of the various applications is to construct a more efficient microbial fuel cell (MFC). MFC is a machine which uses electrons produced by microorganism to generate electricity. If we genetically modify the bacteria Azotobacter vinelandii to have magnetosomes, magnetosomes inside them would be attracted towards the electrodes by magnetic force and in the process, bringing the whole bacteria along with it. In consequence, the physical distance between the bacteria and electrodes will be shortened. As a result, this will increase the efficiency of the MFC as the diffusion rate for the electron to the electrode can be greatly increased.</p></font> |
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− | <p><font face="Times New Roman" size="4pt">Additionally, in the review of the | + | <p><font face="Times New Roman" size="4pt">Additionally, in the review of the previous iGEM teams, the idea of constructing an MFC has been popular. For example, the iGEM 2013 Bielefeld-Germany team also constructed an MFC. After a brief study of their project, we understood that one of their manipulated parts is the oprF gene, which has the biobrick code - k1172501. The team has claimed that oprF, an outer membrane porin, could increase the efficiency of MFC by allowing electron shuttle-mediated extracellular electron transfer from bacteria to electrodes. </p></font> |
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<p><font face="Times New Roman" size="5pt">Investigation on K1172501</p></font> | <p><font face="Times New Roman" size="5pt">Investigation on K1172501</p></font> | ||
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− | <p><font face="Times New Roman" size="4pt">However, with a further study, we have found that the translated sequence exists many stop codons in all of the wrong places. Moreover, with a careful checking of the sequence in silico, it is concluded that the sequence of the biobrick - k1172501 provided by the Bielefeld-Germany team will not be able to translate into an | + | <p><font face="Times New Roman" size="4pt">However, with a further study, we have found that the translated sequence exists many stop codons in all of the wrong places. Moreover, with a careful checking of the sequence in silico, it is concluded that the sequence of the biobrick - k1172501 provided by the Bielefeld-Germany team will not be able to translate into an oprF porin protein. As the DNA sequences of K1172501 is greatly different from oprF DNA sequence from Pseudomonas fluorescens which is the bacteria Germany team claimed to obtain oprF gene sequence.</p></font> |
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− | <p><font face="Times New Roman" size="5pt">OprF in | + | <p><font face="Times New Roman" size="5pt">OprF in Azotobacter vinelandii</p></font> |
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− | <p><font face="Times New Roman" size="4pt">We have found that OprF exists on the outer membrane of | + | <p><font face="Times New Roman" size="4pt">We have found that OprF exists on the outer membrane of Azotobacter vinelandii, which is the bacteria we have been working on. Therefore we chose the Azotobacter vinelandii to provide an alternative source of OprF. The sequence provided by theAzotobacter vinelandii can be completely translated to form OprF with no stop codon appearing in the gene excepting in the last residue. For this, we provide the biobrick, [http://parts.igem.org/Part:BBa_K1648045 BBa_K1648045] and we are planning to provide BBa_K1648047 for insertion of different promoter.</p></font> |
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Revision as of 22:36, 18 September 2015