Difference between revisions of "Team:Jilin China/Collaborations"

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   iGEM encourages  us to communicate and cooperate with other teams. We found that the project  conducted by Wuhan University last year is meaningful and of significance to  environment and human health. Unfortunately, they didn&rsquo;t finish their project  successfully. Therefore, we analyze Wuhan University&rsquo;s last project and make  some improvements this year. They are also willing to collaborate with us and had  already sent us their pSB1C3 vector in their detection system. We start our  project based on this part.  </p>
 
   iGEM encourages  us to communicate and cooperate with other teams. We found that the project  conducted by Wuhan University last year is meaningful and of significance to  environment and human health. Unfortunately, they didn&rsquo;t finish their project  successfully. Therefore, we analyze Wuhan University&rsquo;s last project and make  some improvements this year. They are also willing to collaborate with us and had  already sent us their pSB1C3 vector in their detection system. We start our  project based on this part.  </p>
 
<p><strong>Purpose:</strong><br>
 
<p><strong>Purpose:</strong><br>
   After our confirming experiment, we found that the reason for their  failure is mainly because the hxlR promoter&rsquo;s starting ability is too weak and  their system will do harm to the bacteria&rsquo;s metabolic system. So we decided to  change the promoter in their cleaning system and we add T7 RNA polymerase gene  in the detection system (click here to see more details about  WHU-China 2014 project). By doing this, we are  able to reduce the unnecessary expression in the cells and make the whole  system work well. </p>
+
   After our confirming experiment, we found that the reason for their  failure is mainly because the hxlR promoter&rsquo;s starting ability is too weak and  their system will do harm to the bacteria&rsquo;s metabolic system. So we decided to  change the promoter in their cleaning system and we add T7 RNA polymerase gene  in the detection system (click <a href="https://2014.igem.org/Team:WHU-China">here</a> to see more details about  WHU-China 2014 project). By doing this, we are  able to reduce the unnecessary expression in the cells and make the whole  system work well. </p>
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
 
<p><strong>Materials:</strong></p>
 
<p><strong>Materials:</strong></p>

Revision as of 22:45, 18 September 2015



collaborations

Improve the project of WHU-China in2014

Experiment background:
iGEM encourages us to communicate and cooperate with other teams. We found that the project conducted by Wuhan University last year is meaningful and of significance to environment and human health. Unfortunately, they didn’t finish their project successfully. Therefore, we analyze Wuhan University’s last project and make some improvements this year. They are also willing to collaborate with us and had already sent us their pSB1C3 vector in their detection system. We start our project based on this part. 

Purpose:
After our confirming experiment, we found that the reason for their failure is mainly because the hxlR promoter’s starting ability is too weak and their system will do harm to the bacteria’s metabolic system. So we decided to change the promoter in their cleaning system and we add T7 RNA polymerase gene in the detection system (click here to see more details about  WHU-China 2014 project). By doing this, we are able to reduce the unnecessary expression in the cells and make the whole system work well.

 

Materials:


Name

Source

pSB1C3 vector

Wuhan University

T7 RNA polymerase

Pro.Gao’s lab

FDH

Pro.Gao’s lab

PADH

Pro.Gao’s lab