Difference between revisions of "Team:UCSC/Parts"

Line 257: Line 257:
 
<h1> Parts</h2>
 
<h1> Parts</h2>
 
<p style="font-family: Courier;">
 
<p style="font-family: Courier;">
−
>Natural halophilic protein linker 1
+
<b>>Natural halophilic protein linker 1</b>
 
<br/>
 
<br/>
 
CGGCGAGAGAACATGCTGTGGTTTCGAGTGCCC
 
CGGCGAGAGAACATGCTGTGGTTTCGAGTGCCC
 
<br/>
 
<br/>
−
>Natural halophilic protein linker 2
+
<br/>
 +
<b>>Natural halophilic protein linker 2</b>
 
<br/>
 
<br/>
 
CGAGCCGAGAACATGCTGTGGCACAAACTTCCC
 
CGAGCCGAGAACATGCTGTGGCACAAACTTCCC
 
<br/>
 
<br/>
−
>Engineered rigid halophilic protein linker
+
<br/>
 +
<b>>Engineered rigid halophilic protein linker</b>
 
<br/>
 
<br/>
 
GAAGCTGCTGCTAAAGAAGCTGCTGCTAAA
 
GAAGCTGCTGCTAAAGAAGCTGCTGCTAAA
 
<br/>
 
<br/>
−
>Engineered flexible halophilic protein linker
+
<br/>
 +
<b>>Engineered flexible halophilic protein linker</b>
 
<br/>
 
<br/>
 
GGAGGAGGAGGAAGTGGAGGAGGAGGAAGT
 
GGAGGAGGAGGAAGTGGAGGAGGAGGAAGT
 
<br/>
 
<br/>
−
>Codon Optimized Shewanella Sediminis Alcohol Dehydrogenase for expression in Haloferax Volcanii
+
<br/>
 +
<b>>Codon Optimized Shewanella Sediminis Alcohol Dehydrogenase for expression in Haloferax Volcanii</b>
 
<br/>
 
<br/>
 
ATGACGGTCACGAACGAGCAAGAGCTCGATCTGCTGGTCCAGCGAGTGGCCGATGCGCAG
 
ATGACGGTCACGAACGAGCAAGAGCTCGATCTGCTGGTCCAGCGAGTGGCCGATGCGCAG
Line 318: Line 322:
 
TTTCTGGCCAAACTCGATGAACTCGCCGAGGACGCGTTTGACGATCAATGTACGGGCGCC
 
TTTCTGGCCAAACTCGATGAACTCGCCGAGGACGCGTTTGACGATCAATGTACGGGCGCC
 
AATCCGCGCTACCCGCTGATTGAGGAACTGAAGCAAGTCCTCCTCGCGTCGTTCTACGGC
 
AATCCGCGCTACCCGCTGATTGAGGAACTGAAGCAAGTCCTCCTCGCGTCGTTCTACGGC
 +
<br/>
 
ACGGCCTATTCGGACAAGTGA
 
ACGGCCTATTCGGACAAGTGA
 
<br/>
 
<br/>
−
>Codon optimized Zymomonas mobilis pyruvate decarboxylase for expression in Haloferax Volcanii
+
<br/>
 +
<b>>Codon optimized Zymomonas mobilis pyruvate decarboxylase for expression in Haloferax Volcanii</b>
 
<br/>
 
<br/>
 
ATGTCGTACACGGTCGGCACGTACCTCGCCGAGCGCCTCGTCCAGATCGGCCTCAAACAC
 
ATGTCGTACACGGTCGGCACGTACCTCGCCGAGCGCCTCGTCCAGATCGGCCTCAAACAC
Line 350: Line 356:
 
AGGCCATCAAGGTCGCGCTGGCGAATACGGATGGCCCGACGCTCATCGAGTGCTTCATTG
 
AGGCCATCAAGGTCGCGCTGGCGAATACGGATGGCCCGACGCTCATCGAGTGCTTCATTG
 
GCCGCGAAGACTGCACGGAAGAACTCGTCAAATGGGGCAAGCGCGTGGCCGCCGCCAATA
 
GCCGCGAAGACTGCACGGAAGAACTCGTCAAATGGGGCAAGCGCGTGGCCGCCGCCAATA
 +
<br/>
 
GCCGCAAACCGGTCAACAAGCTCCTCTGA
 
GCCGCAAACCGGTCAACAAGCTCCTCTGA
 
<br/>
 
<br/>
−
>Codon Optimized Haloarcula Hispanica beta-glucosidase
+
<br/>
 +
<b>>Codon Optimized Haloarcula Hispanica beta-glucosidase</b>
 
<br/>
 
<br/>
 
ATGGCACATGACACGACTGACGACGGTATGCAGGCGTCTCGGCGGACATTTCTGAAAGCG
 
ATGGCACATGACACGACTGACGACGGTATGCAGGCGTCTCGGCGGACATTTCTGAAAGCG
Line 396: Line 404:
 
GCGTCGATCACGGATCCCGATCCGGTGCCGGGTCTGCCGGACTGGTCCGGCTCCACGCCC
 
GCGTCGATCACGGATCCCGATCCGGTGCCGGGTCTGCCGGACTGGTCCGGCTCCACGCCC
 
GGTCGCGGCAACTCGGGCAACAACGGCAACGGTAACAATACGTCGCGAGACCCCACGATG
 
GGTCGCGGCAACTCGGGCAACAACGGCAACGGTAACAATACGTCGCGAGACCCCACGATG
 +
<br/>
 
GAGGATGTCGTGGACCTCCTGGATGAGGTCCGCGGTCGCTCGTAG
 
GAGGATGTCGTGGACCTCCTGGATGAGGTCCGCGGTCGCTCGTAG
 
</p>
 
</p>

Revision as of 22:52, 18 September 2015

Parts

>Natural halophilic protein linker 1
CGGCGAGAGAACATGCTGTGGTTTCGAGTGCCC

>Natural halophilic protein linker 2
CGAGCCGAGAACATGCTGTGGCACAAACTTCCC

>Engineered rigid halophilic protein linker
GAAGCTGCTGCTAAAGAAGCTGCTGCTAAA

>Engineered flexible halophilic protein linker
GGAGGAGGAGGAAGTGGAGGAGGAGGAAGT

>Codon Optimized Shewanella Sediminis Alcohol Dehydrogenase for expression in Haloferax Volcanii
ATGACGGTCACGAACGAGCAAGAGCTCGATCTGCTGGTCCAGCGAGTGGCCGATGCGCAG GCCGAGTACGCCAATTTCTCGCAGGAGCAGGTGGACAAGATTTTCCGAGCGGCGGCGCTG GCGGCCGCGGATGCCCGGATCAGCCTCGCGAAGATGGCGGCGAATGAAACGGGCATGGGC GTGATCGAGGACAAGGTCATCAAGAATCACTTCGCCTCGGAGTACATTTACAACAAGTAC AAGGATGAGAAAACCTGTGGCATTCTGGACGAGGATCCCACGTTCGGCACGATCACCATC GCGGAGCCGGTCGGTATCATTTGCGGGATCGTCCCGACGACGAACCCCACCTCCACGGCG ATCTTTAAGGCCCTGATTTCGCTCAAGACCCGCAACGGCATCATCTTCTCGCCGCACCCC CGAGCCAAGGAGTCCACCACGACGGCGGCCCGGCTCGTCCTGAATGCCGCGATCGAAGCG GGTGCGCCCAAGGATATTATCGGGTGGATCGATGAGCCGTCGGTGGCCCTGTCGAACCAG CTCATGACGCATGATAAGATTAACCTCATTCTCGCCACCGGCGGTCCCGGTATGGTGAAG GCCGCCTACTCGTCGGGGAAGCCGGCCATTGGGGTGGGTGCCGGGAACACCCCCATCGTC ATTGACGAAACCGCGGACATCAAGCGCGCGGTGTCGTCCATCCTCATGTCCAAGACCTTT GACAACGGGGTGGTGTGCGCGTCGGAGCAAGCCGTGGTCATTGTCGACGAAATTTACGAT ACGGTCAAGGAGCGGTTTGCCACGCACGGGGGCTACATTCTCAGCAAAGACGAGACCGCC GCCATGCAAAATGTCATTCTGAAGAATGGCGGGCTCAACGCGGATATCGTCGGTCAGAGC GCCGTGACCATTGCGGCGATGGCCGACATTGAGGTCCCCTCGTGGACGAAGGTCCTGATT GGTGAGGTCGAGGATGTCTCGGAGGCGGAGGCCTTTGCGCATGAGAAGCTCAGCCCGCTG CTGGGCATGTACCGCGTGAAGGACTTTGAAGCCGCCATGGACAAAGCCGAAGCCCTGGTC ACCCTCGGGGGTATCGGTCACACGAGCGGTCTGTACACGAACCAAGACACGCAGACGGAA CGCGTGATGGCGTTTGGCTTCCGCATGAAGACGGCGCGCATCCTGATCAACACGCCGGCG TCCCAAGGTGGTATTGGCGACCTGTATAACTTTAAGCTGGCCCCCTCGCTCACGCTGGGC TGCGGCAGCTGGGGTGGGAATTCGATTAGCGAGAACGTCGGCCCGTCGCACCTCATCAAT AAGAAAATGGTCGCGAAACGCGCGGAGAACATGCTCTGGCACAAGCTCCCGTCGTCGATC TACTTCCGACGGGGGTCGCTCCCGATTGCCCTCGAAGAACTCAGCGATAAAAAGCGAGCG CTGATCGTCACGGATAAGTACCTCTTTAATAACGGCTATTGCGATGAGACCATCAAAATT CTCAAGTCGCAGGGGCTCGAAACGGAGATCTTCTACGAAGTGGAAGCGGACCCGACGCTC GCGATTGTCACGCAGGGCGCGAAGATGGCCCAATCGTTCCAGCCGGATGTGATCATTGCC CTGGGTGGGGGCAGCCCGATGGATGCCGCCAAGATTATTTGGGTCATGTACGAGCACCCG GACGTCGATTTTGCGGACCTCGCCCTCCGGTTTATGGACATTCGCAAGCGCATCTACAAG TTCCCGAAACTGGGGGTCAAAGCGCAGATGGTGGCCATCCCGACCACGTCGGGCACCGGC AGCGAGGTGACGCCGTTCGCGGTCGTCACCGATGAAAAGACGGGTATGAAGTACCCCATT GCCGATTATCAACTGACGCCCAACATGGCGATCGTGGATCCGAACCTGGTGATGGATATG CCGAAGTCGCTGACCGCCTTCGGGGGGATTGACGCGGTGACCCACGCGCTCGAGGCCTAC GTCTCGGTCATGGCCAACGAATACTCGGACGGCCAGGCGCTCCAAGCGCTGGATCTCCTC TTCAAGCACCTCCCGGACAGCTACAACCACGGTGCCGCGGCGCCCATCGCCCGGGAAAAG GTCCACAATGGCGCGACGATTGCGGGGATCGCGTTCGCCAACGCCTTCCTCGGGATTTGT CACAGCATGGCCCATAAGCTGGGGGCCGAATTTCACCTGGCCCACGGGCTCGCCAATGCC CTGCTCATTAACAACGTCATCCGGTTCAACGCGACGGACATGCCGACGAAGCAGGCGGCG TTTTCGCAGTACGATCGACCCAAGGCGCTGTGCCGCTACGCGAAAATCGCCGAATATCTG AACCTGGGCGGTACGACGGATGAGGAGAAGGTGGAGGCCCTCATTGAAGAAGTCGACCAG CTGAAAAAAACCATTGGTATTCCGGCGAGCATTCGAGAGGCGGGTGTCAACGAGGCCGAC TTTCTGGCCAAACTCGATGAACTCGCCGAGGACGCGTTTGACGATCAATGTACGGGCGCC AATCCGCGCTACCCGCTGATTGAGGAACTGAAGCAAGTCCTCCTCGCGTCGTTCTACGGC
ACGGCCTATTCGGACAAGTGA

>Codon optimized Zymomonas mobilis pyruvate decarboxylase for expression in Haloferax Volcanii
ATGTCGTACACGGTCGGCACGTACCTCGCCGAGCGCCTCGTCCAGATCGGCCTCAAACAC CACTTCGCGGTGGCCGGAGATTACAACCTGGTCCTGCTCGACAACCTCCTCCTCAACAAA AACATGGAGCAGGTCTACTGCTGCAACGAACTGAATTGCGGTTTTTCCGCCGAGGGCTAC GCGCGAGCGAAAGGTGCCGCGGCGGCGGTCGTGACGTACTCGGTCGGGGCCCTGTCGGCC TTCGACGCGATCGGAGGAGCGTATGCGGAGAACCTCCCCGTCATCCTGATCTCGGGTGCG CCGAATAACAACGATCATGCCGCCGGTCACGTCCTCCACCACGCCCTGGGCAAGACGGAT TACCACTACCAGCTCGAAATGGCGAAGAACATTACCGCCGCCGCGGAAGCCATCTACACG CCCGAAGAGGCGCCGGCCAAGATTGATCATGTCATTAAGACGGCCCTGCGAGAGAAAAAG CCGGTCTACCTCGAGATCGCCTGTAACATTGCCTCGATGCCGTGTGCCGCCCCCGGTCCC GCGTCGGCGCTCTTCAATGATGAAGCCTCGGACGAAGCGTCGCTCAATGCGGCCGTCGAG GAGACGCTGAAATTCATCGCCAACCGCGACAAGGTGGCGGTCCTCGTCGGCTCGAAGCTG CGAGCCGCGGGTGCCGAGGAAGCCGCCGTCAAGTTCGCGGATGCGCTGGGGGGAGCGGTC GCCACGATGGCGGCCGCGAAAAGCTTCTTCCCGGAAGAGAACCCGCACTATATCGGTACG TCGTGGGGTGAGGTCTCCTATCCGGGTGTGGAGAAGACGATGAAGGAGGCGGACGCGGTC ATCGCGCGCTCGCCCCGGTGTTCAACGACTACAGCACCACGGGCTGGACGGACATCCCGG ACCCGAAAAAACTCGTGCTCGCGGAGCCGCGATCCGTCGTCGTGAATGGCATTCGCTTCC CGTCGGTCCACCTCAAGGACTACCTCACGCGCCTGGCCCAAAAGGTCTCGAAGAAAACCG GAGCCCTGGACTTCTTCAAGTCGCTGAATGCCGGTGAACTCAAGAAAGCGGCGCCCGCGG ATCCCTCCGCCCCGCTCGTGAACGCGGAAATCGCCCGCCAGGTCGAAGCGCTCCTGACGC CGAACACGACGGTGATTGCCGAGACGGGCGACTCGTGGTTCAATGCCCAACGCATGAAGC TCCCGAACGGAGCGCGGGTCGAATACGAGATGCAGTGGGGCCACATCGGCTGGTCGGTGC CGGCCGCCTTCGGCTATGCGGTGGGAGCCCCGGAACGGCGAAATATTCTCATGGTCGGCG ACGGCTCGTTTCAGCTCACGGCGCAGGAGGTCGCCCAGATGGTCCGACTGAAACTCCCGG TCATTATCTTCCTCATCAACAACTACGGCTACACGATCGAGGTCATGATCCATGATGGGC CCTACAACAACATCAAAAACTGGGACTACGCGGGCCTGATGGAAGTCTTCAACGGCAATG GCGGATACGACAGCGGTGCGGGCAAGGGTCTCAAAGCGAAAACGGGTGGCGAACTGGCCG AGGCCATCAAGGTCGCGCTGGCGAATACGGATGGCCCGACGCTCATCGAGTGCTTCATTG GCCGCGAAGACTGCACGGAAGAACTCGTCAAATGGGGCAAGCGCGTGGCCGCCGCCAATA
GCCGCAAACCGGTCAACAAGCTCCTCTGA

>Codon Optimized Haloarcula Hispanica beta-glucosidase
ATGGCACATGACACGACTGACGACGGTATGCAGGCGTCTCGGCGGACATTTCTGAAAGCG ACCGGTGTGGCCACGGCAGCGGCCGCCACCGGCGCAGGTGTTGGGACCGCCGCGGCACAA CCGGACGTCGACAGTCTCATTGGCGACCTCACCCTCGAGCAGAAAGCGGCCCAGATGACG CAGGTCGCGATCTCGTCGTTCGAAGCCGAACCGGAAGAGTCGAATGTGCCGGATTCGTTT GGGGTCGACACGGTCGGCGAGTACTTCAGCGAGCTGGGCATCGGGTCGATCCTGTCGGGT GGCGCGGAACCGCCGTCGTTTGACGCGAACGCGGTCGTCCAGGGGATCAACGCCCTGCAG CAATACAACCTCGACAACGCGGACCACGACATCCCGTTCCTCTACGGGGTGGATGCGACG CACGGCAACGGCCTCCTGGAAGGGGCCACCGTCTTCCCGCAACGCCTCAACATGGGCGTC ACGCGAGATCTGTCGCTCATCGCCGAAGCGGAACGCCACACGTCGGACGCCACGGCGAGC ATGGGTGCCCATTGGACCTTTGCGCCGACGACGGACCTCCAACGCGACCCGCGCTGGGGC CGATTTTTCGAAGGCATCTCGGAAGACCCGAAGCTGGAGGCGGATGTCAGCCGAGCCCGA GCGCGAGCCCTCGAAGACGACGATCGCCTCACGGCCTGCGTGAAGCACTTCGCGGCGTAC TCGGTGCCGAACAATGGCAATGATCGAGCGCCCGCCAGCACGAGCCTCCGAGACCTCCGC ACGAACATCCTCCCGCCGTACCGAGAGGCGCTGGAATCGGAGCCCGGCACGGTCATGGTC AACTCGGGTAGCATCAACGGCGTGCCCGCCCACGCCTCCCACTGGCTCCTCACGACGCTC CTCCGCGACACGTACGGCTACGAGGGGATGGTCGTGTCGGATTGGGACGATCTCAATCGC ATGATCACGAATCACGACTATGCCCCCGATTTCGAGACGGCGACGGAGATGGCGATCAAT GCGGGCGTCGATATGTATATGATCGGTAACGGTGGGGATGCCCCGGGTCCCGTCCAATTC ATCGACACCGTGGTGTCGCTGGTCGAGGACGGTGCGATCCCGATGGAGCGAATCGACGAG GCGGTCCGCCGCATTCTCGAGCTCAAGGCCGACCTGGGCCTCTTTGAACAACCGACGGTC GACGAATCGCGAATTGAGACGGTCCTGGGTGGTGCGCAGGAGACCGCCGAGACGATGGCG AAAGAGTCGATGGTGCTCCTGAAGAACACCGACGATACGCTCCCGCTCTCGGGCGATGAG TCCGTCCTCCTGACGGGTCCGGGTGTGGACTCGAATGGCAATAATACGCGAGCCCTCATG CAGCATGGTGGTTGGACGCTCGGCTGGCAAGGCGCCAGCGCCGGTGGCCCGTTTCCGCGC CAGAACCTGCTGGAGGCCGAACTCCGAGCGCGAGTCGGCTCCCTCACGCACGTCCCGACG AGCTACGAGAATACCACCTGGTGGGCGGGTGAAGGCGACGGCGAGAACCAGCAGTCGGAT GAAAATGGTAATTTCGACTTCACGGCGGAGCAGCGCTCGCGGGTCGAATCCGCCGGTCCC GAATCGGATGTGGTGGTCGTGGTCCTGGGGGAAGGCACGCACAACGAGGGCTTCGGCGAC CGAGATGAACTCGTGCTCGACGAGTCGCAGCAGGCGCTCCTCGATACCGTGGTGGAATCG ACGGATGACAGCACGCCGATCATCGGGGTCATGCTGGCCGGCTCGCCCCGAGGCTCCCCC GAGACGTTTAGCCAGCTCGATGCCCTCCTCTTTGCGGGTCAACCGGGTAGCGACGGGGGT GTCGCGATTGCGGAGACGCTGGTCGGCGAGTACAACCCCTCGGGTAAGCTCCCGTTCTCG TGGCCGGAGAATGTCGGCACGACCCCGGTCCAGTACACGCGCTACGACCCGACGTCCACG GGTGGTACGGACAACACGGCGATCTACGAATATGGGCACGGCCTGTCGTACACGGATTTC GAGTACGGCTCGGTCAGCGTGACGCAGCCGACCGTCGGCAGCCCGGCCAACCAATCCGAG GTCACCGTGTCGGTGGAGGTGACGAACACGGGTCGCATGGCCGGTGAGCACATTGTCGAG GTCTTCAACACGCAGTCGTATGGGACGGTCCTCCAACCGCGACGACGACTCCTCGGTTAC GAACGCGTCTCGCTGGGTCCCGGGGAACTGACGACCGTGGATGTCACGGTGGATCTCACG GCCCTGGAAGTGGTGCCCGGTGACGTGCTGGGCCTCGGTCCGAAGGTCGTCGAAGCGGGG GAATACGAGCTGACGGTGGGCCAAGACGGCCCGACGGCCACCCTCACCGTCCAGAATACG GCGTCGATCACGGATCCCGATCCGGTGCCGGGTCTGCCGGACTGGTCCGGCTCCACGCCC GGTCGCGGCAACTCGGGCAACAACGGCAACGGTAACAATACGTCGCGAGACCCCACGATG
GAGGATGTCGTGGACCTCCTGGATGAGGTCCGCGGTCGCTCGTAG