Difference between revisions of "Team:Technion HS Israel/Notebook"

 
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<link href="//2015.igem.org/Template:Technion_HS_Israel4/Technion_HS_Israel_menu_style?action=raw&ctype=text/css"  
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<h1><font color="#FF0000">Notebook</font></h1>
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<p> Here is a summery of our notebook. <!--For the full notebook please look at the following scans--!></p>   
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<div id="toc" class="toc">
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<span>Contents</span>
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<ul><font size="4">
 +
<li><a href="#"><span>Top</span></a></li>
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<li><a href="#Summery"><span>Summery</span></a></li>
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<li><a href="#Preparation"><span>Preparation</span></a></li>
 +
<li><a href="#July"><span>July</span></a></li>
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<li><a href="#August"><span>August</span></a></li>
 +
<li><a href="#September"><span>September</span></a></li>
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</ul>
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</div>
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<a name="Summery"><h1><font color="#008080">Notebook</font></h1>
 +
<p> Here is a summery of our notebook. <For the full notebook please look at the following scans></p>   
 
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<img src="https://static.igem.org/mediawiki/2015/3/3e/Technion_HS_Israel_pre6_2.jpg" alt="https://2015.igem.org/File:Technion_HS_Israel_pre6" style="width:456px;  
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<img src="https://static.igem.org/mediawiki/2015/9/94/Technion_HS_Israel_pre7_2.jpg" alt="https://2015.igem.org/File:Technion_HS_Israel_pre7" style="width:456px;  
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<img src="https://static.igem.org/mediawiki/2015/e/e6/Technion_HS_Israel_pre3_2.jpg" alt="https://2015.igem.org/File:Technion_HS_Israel_pre3" style="width:456px;  
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<img src="https://static.igem.org/mediawiki/2015/3/31/Technion_HS_Israel_pre4_2.jpg" alt="https://2015.igem.org/File:Technion_HS_Israel_pre4" style="width:456px;
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<a name="July"><h3>July</h3></a>
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<p>&nbsp;<a href="#July 12th"><span>July 12th</span></a> 
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&nbsp;<a href="#July 13th"><span>July 13th</span></a> 
 +
&nbsp;<a href="#July 15th"><span>July 15th</span></a> 
 +
&nbsp;<a href="#July 20th"><span>July 20th</span></a> 
 +
&nbsp; <a href="#July 22th"><span>July 22th</span></a> 
 +
&nbsp;<a href="#July 29th"><span>July 29th</span></a></p> 
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<a name="July 12th"><h4>July 12th</h4></a>
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<img  src="https://static.igem.org/mediawiki/2015/6/64/Technion_HS_Israel_12_7.jpg"
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alt="Technion_HS_Israel_pre1" style="width:456px;
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height:642px; border:solid; border-width:4px;" >
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<a name="July 13th"><h4>July 13th</h4></a>
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<img  src="https://static.igem.org/mediawiki/2015/5/50/Technion_HS_Israel_13_7.jpg"
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alt="Technion_HS_Israel_pre1" style="width:456px;
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<a name="July 15th"><h4>July 15th</h4></a>
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<img  src="https://static.igem.org/mediawiki/2015/6/6b/Technion_HS_Israel_15_7_1.jpg"
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<img src="https://static.igem.org/mediawiki/2015/6/6b/Technion_HS_Israel_15_7_2.jpg" alt="https://2015.igem.org/File:Technion_HS_Israel_pre5" style="width:456px;  
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height:642px; border:solid; border-width:4px;" >
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<a name="July 20th"><h4>July 20th</h4></a>
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<img  src="https://static.igem.org/mediawiki/2015/6/6b/Technion_HS_Israel_20_7.jpg"
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alt="Technion_HS_Israel_pre1" style="width:456px;
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height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="July 22th"><h4>July 22th</h4></a>
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 +
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<img  src="https://static.igem.org/mediawiki/2015/b/bb/Technion_HS_Israel_22_7_1.jpg"
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alt="Technion_HS_Israel_pre1" style="width:456px;
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height:642px; border:solid; border-width:4px;" >
 +
<!--2/2--!>
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<img src="https://static.igem.org/mediawiki/2015/8/86/Technion_HS_Israel_22_7_2.jpg" alt="https://2015.igem.org/File:Technion_HS_Israel_pre5" style="width:456px;
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height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="July 29th"><h4>July 29th</h4></a>
 +
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<img  src="https://static.igem.org/mediawiki/2015/a/a2/Technion_HS_Israel_29_7.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
 
 +
 
 +
 
 +
<a name="August"><h3>August</h3></a>
 +
 
 +
<p> &nbsp;<a href="#august 2th"><span>August 2th</span></a>
 +
&nbsp;<a href="#august 3th"><span>August 3th</span></a>
 +
&nbsp;<a href="#august 4th"><span>August 4th</span></a>
 +
&nbsp;<a href="#august 5th"><span>August 5th</span></a>
 +
&nbsp;<a href="#august 6th"><span>August 6th</span></a>
 +
&nbsp;<a href="#august 9th"><span>August 9th</span></a>
 +
&nbsp;<a href="#august 10th"><span>August 10th</span></a>
 +
&nbsp;<a href="#august 11th"><span>August 11th</span></a>
 +
&nbsp;<a href="#august 12th"><span>August 12th</span></a>
 +
&nbsp;<a href="#august 13th"><span>August 13th</span></a>
 +
&nbsp;<a href="#august 16th"><span>August 16th</span></a>
 +
&nbsp;<a href="#august 17th"><span>August 17th</span></a>
 +
&nbsp;<a href="#august 18th"><span>August 18th</span></a>
 +
&nbsp;<a href="#august 19th"><span>August 19th</span></a>
 +
&nbsp;<a href="#august 20th"><span>August 20th</span></a>
 +
&nbsp;<a href="#august 23th"><span>August 23th</span></a>
 +
&nbsp;<a href="#august 24th"><span>August 24th</span></a>
 +
&nbsp;<a href="#august 25th"><span>August 25th</span></a>
 +
&nbsp;<a href="#august 26th"><span>August 26th</span></a>
 +
&nbsp;<a href="#august 27th"><span>August 27th</span></a>
 +
&nbsp;<a href="#august 29th"><span>August 29th</span></a>
 +
&nbsp;<a href="#august 30th"><span>August 30th</span></a>
 +
&nbsp;<a href="#august 31th"><span>August 31th</span></a> </p>
 +
 
 +
 
 +
 
 +
 
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 +
 
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 +
 
 +
 
 +
 
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 +
 
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 +
<a name="august 2th"><h4>august 2th</h4></a>
 +
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<img  src="https://static.igem.org/mediawiki/2015/f/f6/Technion_HS_Israel_2_8.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="august 3th"><h4>august 3th</h4></a>
 +
 
 +
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<img  src="https://static.igem.org/mediawiki/2015/8/85/Technion_HS_Israel_3_8_1.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
<!--2/2--!>
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<img src="https://static.igem.org/mediawiki/2015/a/a7/Technion_HS_Israel_3_8_2.jpg" alt="https://2015.igem.org/File:Technion_HS_Israel_pre5" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="august 4th"><h4>august 4th</h4></a>
 +
 
 +
<!--1/2--!>
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<img  src="https://static.igem.org/mediawiki/2015/2/24/Technion_HS_Israel_4_8_1.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
<!--2/2--!>
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<img src="https://static.igem.org/mediawiki/2015/c/ca/Technion_HS_Israel_4_8_2.jpg" alt="https://2015.igem.org/File:Technion_HS_Israel_pre5" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="august 5th"><h4>august 5th</h4></a>
 +
<!--1/1--!>
 +
<img  src="https://static.igem.org/mediawiki/2015/b/b8/Technion_HS_Israel_5_8.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="august 6th"><h4>august 6th</h4></a>
 +
 
 +
<!--1/2--!>
 +
<img  src="https://static.igem.org/mediawiki/2015/0/02/Technion_HS_Israel_6_8_1.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
<!--2/2--!>
 +
<img src="https://static.igem.org/mediawiki/2015/1/12/Technion_HS_Israel_6_8_2.jpg" alt="https://2015.igem.org/File:Technion_HS_Israel_pre5" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="august 9th"><h4>august 9th</h4></a>
 +
<!--1/1--!>
 +
<img  src="https://static.igem.org/mediawiki/2015/3/36/Technion_HS_Israel_9_8.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="august 10th"><h4>august 10th</h4></a>
 +
 
 +
<!--1/2--!>
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<img  src="https://static.igem.org/mediawiki/2015/8/8e/Technion_HS_Israel_10_8_1.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
<!--2/2--!>
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<img src="https://static.igem.org/mediawiki/2015/7/73/Technion_HS_Israel_10_8_2.jpg" alt="https://2015.igem.org/File:Technion_HS_Israel_pre5" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="august 11th"><h4>august 11th</h4></a>
 +
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<img  src="https://static.igem.org/mediawiki/2015/c/ce/Technion_HS_Israel_11_8.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="august 12th"><h4>august 12th</h4></a>
 +
<!--1/1--!>
 +
<img  src="https://static.igem.org/mediawiki/2015/2/22/Technion_HS_Israel_12_8.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="august 13th"><h4>august 13th</h4></a>
 +
<!--1/1--!>
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<img  src="https://static.igem.org/mediawiki/2015/f/f9/Technion_HS_Israel_13_8.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="august 16th"><h4>august 16th</h4></a>
 +
<!--1/1--!>
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<img  src="https://static.igem.org/mediawiki/2015/d/d7/Technion_HS_Israel_16_8.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="august 17th"><h4>august 17th</h4></a>
 +
<!--1/1--!>
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<img  src="https://static.igem.org/mediawiki/2015/2/25/Technion_HS_Israel_17_8.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="august 18th"><h4>august 18th</h4></a>
 +
<!--1/1--!>
 +
<img  src="https://static.igem.org/mediawiki/2015/e/e7/Technion_HS_Israel_18_8.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="august 19th"><h4>august 19th</h4></a>
 +
<!--1/1--!>
 +
<img  src="https://static.igem.org/mediawiki/2015/d/d0/Technion_HS_Israel_19_8.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="august 20th"><h4>august 20th</h4></a>
 +
<!--1/1--!>
 +
<img  src="https://static.igem.org/mediawiki/2015/d/d7/Technion_HS_Israel_20_8.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="august 23th"><h4>august 23th</h4></a>
 +
<!--1/1--!>
 +
<img  src="https://static.igem.org/mediawiki/2015/1/12/Technion_HS_Israel_23_8.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="august 24th"><h4>august 24th</h4></a>
 +
<!--1/1--!>
 +
<img  src="https://static.igem.org/mediawiki/2015/e/ee/Technion_HS_Israel_24_8.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="august 25th"><h4>august 25th</h4></a>
 +
<!--1/1--!>
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<img  src="https://static.igem.org/mediawiki/2015/5/52/Technion_HS_Israel_25_8.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="august 26th"><h4>august 26th</h4></a>
 +
<!--1/1--!>
 +
<img  src="https://static.igem.org/mediawiki/2015/f/fe/Technion_HS_Israel_26_8.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="august 27th"><h4>august 27th</h4></a>
 +
<!--1/1--!>
 +
<img  src="https://static.igem.org/mediawiki/2015/5/56/Technion_HS_Israel_27_8.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="august 29th"><h4>august 29th</h4></a>
 +
<!--1/1--!>
 +
<img  src="https://static.igem.org/mediawiki/2015/d/d7/Technion_HS_Israel_29_8.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="august 30th"><h4>august 30th</h4></a>
 +
<!--1/1--!>
 +
<img  src="https://static.igem.org/mediawiki/2015/b/bb/Technion_HS_Israel_30_8.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="august 31th"><h4>august 31th</h4></a>
 +
<!--1/1--!>
 +
<img  src="https://static.igem.org/mediawiki/2015/1/11/Technion_HS_Israel_31_8.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
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height:642px; border:solid; border-width:4px;" >
 +
 
 +
 
 +
 
 +
<a name="September"><h3>September</h3></a>
 +
 
 +
<p>&nbsp;<a href="#september 1th"><span>September 1th</span></a>
 +
&nbsp;<a href="#september 2th"><span>September 2th</span></a>
 +
&nbsp;<a href="#september 3th"><span>September 3th</span></a>
 +
&nbsp;<a href="#september 5th"><span>September 5th</span></a>
 +
&nbsp;<a href="#september 6th"><span>September 6th</span></a>
 +
&nbsp;<a href="#september 7th"><span>September 7th</span></a> </p>
 +
 
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 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
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 +
 
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 +
<a name="september 1th"><h4>september 1th</h4></a>
 +
<!--1/1--!>
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<img  src="https://static.igem.org/mediawiki/2015/e/e6/Technion_HS_Israel_1_9.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
height:642px; border:solid; border-width:4px;" >
 +
 
 +
<a name="september 2th"><h4>september 2th</h4></a>
 +
<!--1/1--!>
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<img  src="https://static.igem.org/mediawiki/2015/c/c7/Technion_HS_Israel_2_9.jpg"
 +
alt="Technion_HS_Israel_pre1" style="width:456px;
 +
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Latest revision as of 22:55, 18 September 2015

Technion 2015 HS Team's Wiki

Notebook

Here is a summery of our notebook.

Week Design Cloning Experiment Finish new BioBrick
Jul 5th to Jul 9th (2015) Primers : OGEM 1 and 2 to open j09855 plasmid .

Gblock :RBS- Aiia with a Gibson overlabs.		 we got j09855 part from the distribution kit. - -
June 12th to July 16th (2015) Primers : OGEM 3 and 4 to got the k1767001 plasmid without LuxR , after doing the PCR we will get a linear DNA so we put two enzymes in the overlaps of the primers to close the plasmids. PCR to open the j09855 plasmid.Gibson to add Aiia . - BBa_K1767001
Aug. 2nd to Aug. 6th (2015) - We got p0440 part from the distribution kit.

We built BBa_K1767002 and BBa_K1767004 Constructs by restriction enzymes.

PCR to get the k1767001 plasmid without LuxR .		 - BBa_K1767002

BBa_K1767004
Aug. 9th to Aug. 13th (2015) Primers : OGEM 5 and 6 to check if we succeed in the PCR ( Those primers for colony PCR) We got I13521 part from the distribution kit.
We built BBa_K1767003 and BBa_K1767005 Constructs by restriction enzymes.
Colony PCR to check if we have LuxR in the plasmid.		 - BBa_K1767003
BBa_K1767005
Aug. 16th to Aug. 20th (2015) - - AHL experiment #1 -
Aug. 23rd to Aug. 27th (2015) Primers : OGEM 7 and 8 to check if we have yfp in the plasmid ( for colony PCR ). We got I13003 part from the distribution kit. AHL experiment #2 -
Aug. 30th to Sept. 9th (2015) Primers: OGEM 9 and 10 to replaced the RFP with RFP ( with LVA ) We built BBa_K1767009 and BBa_K1767010 Constructs by restriction enzymes.
Colony PCR to check if we have YFP in the plasmid.		 - BBa_K1767009
BBa_K1767010
Sept. 6th to Sept. 11th (2015) - - AHL experiment # 3 -

preparation
  • 1. LB medium
  • 2. Miniprep
Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5 https://2015.igem.org/File:Technion_HS_Israel_pre6 https://2015.igem.org/File:Technion_HS_Israel_pre7 https://2015.igem.org/File:Technion_HS_Israel_pre2 https://2015.igem.org/File:Technion_HS_Israel_pre3 https://2015.igem.org/File:Technion_HS_Israel_pre4

July

 July 12th  July 13th  July 15th  July 20th   July 22th  July 29th

July 12th

 Technion_HS_Israel_pre1

July 13th

 Technion_HS_Israel_pre1

July 15th

 Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5

July 20th

 Technion_HS_Israel_pre1

July 22th

 Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5

July 29th

 Technion_HS_Israel_pre1

August

 August 2th  August 3th  August 4th  August 5th  August 6th  August 9th  August 10th  August 11th  August 12th  August 13th  August 16th  August 17th  August 18th  August 19th  August 20th  August 23th  August 24th  August 25th  August 26th  August 27th  August 29th  August 30th  August 31th

august 2th

 Technion_HS_Israel_pre1

august 3th

 Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5

august 4th

 Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5

august 5th

 Technion_HS_Israel_pre1

august 6th

 Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5

august 9th

 Technion_HS_Israel_pre1

august 10th

 Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5

august 11th

 Technion_HS_Israel_pre1

august 12th

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august 13th

 Technion_HS_Israel_pre1

august 16th

 Technion_HS_Israel_pre1

august 17th

 Technion_HS_Israel_pre1

august 18th

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august 19th

 Technion_HS_Israel_pre1

august 20th

 Technion_HS_Israel_pre1

august 23th

 Technion_HS_Israel_pre1

august 24th

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august 25th

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august 26th

 Technion_HS_Israel_pre1

august 27th

 Technion_HS_Israel_pre1

august 29th

 Technion_HS_Israel_pre1

august 30th

 Technion_HS_Israel_pre1

august 31th

 Technion_HS_Israel_pre1

September

 September 1th  September 2th  September 3th  September 5th  September 6th  September 7th

september 1th

 Technion_HS_Israel_pre1

september 2th

 Technion_HS_Israel_pre1

september 3th

 Technion_HS_Israel_pre1

september 5th

 Technion_HS_Israel_pre1

september 6th

 Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5

september 7th

 Technion_HS_Israel_pre1