Difference between revisions of "Team:Technion HS Israel/Notebook"

 
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<h1><font color="#FF0000">Notebook</font></h1>
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<p> Here is a summery of our notebook. <!--For the full notebook please look at the following scans--!></p> 
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<span>Contants</span>
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<span>Contents</span>
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<ul>
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<ul><font size="4">
 
<li><a href="#"><span>Top</span></a></li>
 
<li><a href="#"><span>Top</span></a></li>
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<li><a href="#Technion_HS_Israel_pre1"><span>NanoBio Protocol for gene knockout</span></a></li>
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<li><a href="#Summery"><span>Summery</span></a></li>
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<li><a href="#Antibiotics preparation"<span>Antibiotics preparation</span></a><li>
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<li><a href="#Preparation"><span>Preparation</span></a></li>
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<li><a href="#Chemical transformation"><span>Chemical transformation</span></a></li>
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<li><a href="#July"><span>July</span></a></li>
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<li><a href="#DNA_Kit_Plate"><span>DNA Kit Plate</span></a></li>
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<li><a href="#August"><span>August</span></a></li>
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<li><a href="#Gel_and_PCR_Extraction"><span>Gel and PCR Extraction</span></a></li>
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<li><a href="#September"><span>September</span></a></li>
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<li><a href="#Gel Prep"><span>Gel Prep</span></a></li>
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<li><a href="#Glycerol stock"><span>Glycerol stock</span></a></li>
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<li><a href="#LB SOB SOC BA"><span>LB SOB SOC BA</span></a></li>
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<li><a href="#Ligation"><span>Ligation</span></a></li>
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<li><a href="#manual NEB gibson assembly"><span>manual NEB (gibson assembly) </span></a></li>
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<li><a href="#MixandGo"><span>MixandGo</span></a></li>
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<li><a href="#PCR"><span>PCR</span></a></li>
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<li><a href="#Phosphorylation and blunt ligation"><span>Phosphorylation and blunt ligation</span></a></li>
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<li><a href="#Presto Mini Plasmid Kit Protocol"><span>Presto Mini Plasmid Kit Protocol</span></a></li>
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<li><a href="#Restriction enzyme"><span>Restriction enzyme</span></a></li>
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<li><a href="#Transformation to 3300 LG using electroporation"><span>Transformation to 3300 LG using electroporation</span></a></li>
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<li><a href="#AHL inducer experiment protocol"><span>AHL inducer experiment protocol </span></a></li>
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</ul>
 
</ul>
 
</div>
 
</div>
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<a name="Summery"><h1><font color="#008080">Notebook</font></h1>
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<p> Here is a summery of our notebook. <For the full notebook please look at the following scans></p> 
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   <th><font color="#FF3399">Week</font></th>
 
   <th><font color="#FF3399">Week</font></th>
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<a name="Preparation"><h4>preparation</h4></a>
 
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<li>1. LB medium</li>
 
<li>1. LB medium</li>
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<img src="https://static.igem.org/mediawiki/2015/3/31/Technion_HS_Israel_pre4_2.jpg" alt="https://2015.igem.org/File:Technion_HS_Israel_pre4" style="width:456px;  
 
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<a name="July"><h3>July</h3></a>
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<p>&nbsp;<a href="#July 12th"><span>July 12th</span></a> 
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&nbsp;<a href="#July 13th"><span>July 13th</span></a> 
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&nbsp;<a href="#July 15th"><span>July 15th</span></a> 
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&nbsp;<a href="#July 20th"><span>July 20th</span></a> 
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&nbsp; <a href="#July 22th"><span>July 22th</span></a> 
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&nbsp;<a href="#July 29th"><span>July 29th</span></a></p> 
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<a name="July 12th"><h4>July 12th</h4></a>
 
<a name="July 12th"><h4>July 12th</h4></a>
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<a name="August"><h3>August</h3></a>
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<p> &nbsp;<a href="#august 2th"><span>August 2th</span></a>
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&nbsp;<a href="#august 3th"><span>August 3th</span></a>
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&nbsp;<a href="#august 4th"><span>August 4th</span></a>
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&nbsp;<a href="#august 5th"><span>August 5th</span></a>
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&nbsp;<a href="#august 6th"><span>August 6th</span></a>
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&nbsp;<a href="#august 9th"><span>August 9th</span></a>
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&nbsp;<a href="#august 10th"><span>August 10th</span></a>
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&nbsp;<a href="#august 11th"><span>August 11th</span></a>
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&nbsp;<a href="#august 12th"><span>August 12th</span></a>
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&nbsp;<a href="#august 13th"><span>August 13th</span></a>
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&nbsp;<a href="#august 16th"><span>August 16th</span></a>
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&nbsp;<a href="#august 17th"><span>August 17th</span></a>
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&nbsp;<a href="#august 18th"><span>August 18th</span></a>
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&nbsp;<a href="#august 19th"><span>August 19th</span></a>
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&nbsp;<a href="#august 20th"><span>August 20th</span></a>
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&nbsp;<a href="#august 23th"><span>August 23th</span></a>
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&nbsp;<a href="#august 24th"><span>August 24th</span></a>
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&nbsp;<a href="#august 25th"><span>August 25th</span></a>
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&nbsp;<a href="#august 26th"><span>August 26th</span></a>
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&nbsp;<a href="#august 27th"><span>August 27th</span></a>
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&nbsp;<a href="#august 29th"><span>August 29th</span></a>
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&nbsp;<a href="#august 30th"><span>August 30th</span></a>
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&nbsp;<a href="#august 31th"><span>August 31th</span></a> </p>
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<a name="august 2th"><h4>august 2th</h4></a>
 
<a name="august 2th"><h4>august 2th</h4></a>
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<a name="September"><h3>September</h3></a>
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<p>&nbsp;<a href="#september 1th"><span>September 1th</span></a>
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&nbsp;<a href="#september 2th"><span>September 2th</span></a>
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&nbsp;<a href="#september 3th"><span>September 3th</span></a>
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&nbsp;<a href="#september 5th"><span>September 5th</span></a>
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&nbsp;<a href="#september 6th"><span>September 6th</span></a>
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&nbsp;<a href="#september 7th"><span>September 7th</span></a> </p>
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<a name="september 1th"><h4>september 1th</h4></a>
 
<a name="september 1th"><h4>september 1th</h4></a>

Latest revision as of 22:55, 18 September 2015

Technion 2015 HS Team's Wiki

Notebook

Here is a summery of our notebook.

Week Design Cloning Experiment Finish new BioBrick
Jul 5th to Jul 9th (2015) Primers : OGEM 1 and 2 to open j09855 plasmid .

Gblock :RBS- Aiia with a Gibson overlabs.		 we got j09855 part from the distribution kit. - -
June 12th to July 16th (2015) Primers : OGEM 3 and 4 to got the k1767001 plasmid without LuxR , after doing the PCR we will get a linear DNA so we put two enzymes in the overlaps of the primers to close the plasmids. PCR to open the j09855 plasmid.Gibson to add Aiia . - BBa_K1767001
Aug. 2nd to Aug. 6th (2015) - We got p0440 part from the distribution kit.

We built BBa_K1767002 and BBa_K1767004 Constructs by restriction enzymes.

PCR to get the k1767001 plasmid without LuxR .		 - BBa_K1767002

BBa_K1767004
Aug. 9th to Aug. 13th (2015) Primers : OGEM 5 and 6 to check if we succeed in the PCR ( Those primers for colony PCR) We got I13521 part from the distribution kit.
We built BBa_K1767003 and BBa_K1767005 Constructs by restriction enzymes.
Colony PCR to check if we have LuxR in the plasmid.		 - BBa_K1767003
BBa_K1767005
Aug. 16th to Aug. 20th (2015) - - AHL experiment #1 -
Aug. 23rd to Aug. 27th (2015) Primers : OGEM 7 and 8 to check if we have yfp in the plasmid ( for colony PCR ). We got I13003 part from the distribution kit. AHL experiment #2 -
Aug. 30th to Sept. 9th (2015) Primers: OGEM 9 and 10 to replaced the RFP with RFP ( with LVA ) We built BBa_K1767009 and BBa_K1767010 Constructs by restriction enzymes.
Colony PCR to check if we have YFP in the plasmid.		 - BBa_K1767009
BBa_K1767010
Sept. 6th to Sept. 11th (2015) - - AHL experiment # 3 -

preparation
  • 1. LB medium
  • 2. Miniprep
Technion_HS_Israel_pre1 https://2015.igem.org/File:Technion_HS_Israel_pre5 https://2015.igem.org/File:Technion_HS_Israel_pre6 https://2015.igem.org/File:Technion_HS_Israel_pre7 https://2015.igem.org/File:Technion_HS_Israel_pre2 https://2015.igem.org/File:Technion_HS_Israel_pre3 https://2015.igem.org/File:Technion_HS_Israel_pre4

July

 July 12th  July 13th  July 15th  July 20th   July 22th  July 29th

July 12th

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July 13th

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July 15th

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July 20th

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July 22th

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July 29th

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August

 August 2th  August 3th  August 4th  August 5th  August 6th  August 9th  August 10th  August 11th  August 12th  August 13th  August 16th  August 17th  August 18th  August 19th  August 20th  August 23th  August 24th  August 25th  August 26th  August 27th  August 29th  August 30th  August 31th

august 2th

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august 3th

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august 4th

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august 5th

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august 6th

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august 9th

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august 10th

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august 11th

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august 12th

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august 13th

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august 16th

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august 17th

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august 18th

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august 19th

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august 20th

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august 23th

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august 24th

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august 25th

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august 26th

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august 27th

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august 29th

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august 30th

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august 31th

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September

 September 1th  September 2th  September 3th  September 5th  September 6th  September 7th

september 1th

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september 2th

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september 3th

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september 5th

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september 6th

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september 7th

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