Difference between revisions of "Team:Northeastern Boston/BasicParts"
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<div class='wrapper'> | <div class='wrapper'> | ||
<div id='sidebar'> | <div id='sidebar'> | ||
| − | <div id='bar_title' class='title'> | + | <div id='bar_title' class='title'><a href="https://2015.igem.org/Team:Northeastern_Boston"> |
| − | Northeastern | + | Northeastern</a> |
</div> | </div> | ||
<ul class='nav'> | <ul class='nav'> | ||
<li> | <li> | ||
| − | <a | + | <a id='a-home' href="https://2015.igem.org/Team:Northeastern_Boston">Home</a> |
</li> | </li> | ||
<li> | <li> | ||
| − | <a href="https://2015.igem.org/Team:Northeastern_Boston/ | + | <a id='a-team' href="https://2015.igem.org/Team:Northeastern_Boston/Attributions">Team</a> |
</li> | </li> | ||
<li> | <li> | ||
| − | <a href="https://2015.igem.org/Team:Northeastern_Boston/ | + | <a id='a-project' href="https://2015.igem.org/Team:Northeastern_Boston/Description">Project</a> |
</li> | </li> | ||
<div class='sub-nav__container'> | <div class='sub-nav__container'> | ||
| − | <li class='sub-nav__parent'> | + | <li id='a-parts' class='sub-nav__parent'> |
| − | <a>Parts</a> | + | <a> |
| + | Parts | ||
| + | <div class='nav-container__arrow flip-vertical'></div> | ||
| + | </a> | ||
</li> | </li> | ||
| − | <div class='sub-nav__children | + | <div class='sub-nav__children'> |
<li class='sub-nav__child'> | <li class='sub-nav__child'> | ||
| − | <a>Basic & Composite</a> | + | <a class='active'>Basic & Composite</a> |
</li> | </li> | ||
<li class='sub-nav__child'> | <li class='sub-nav__child'> | ||
| − | <a>Expression Plasmid</a> | + | <a href="https://2015.igem.org/Team:Northeastern_Boston/Design">Expression Plasmid</a> |
</li> | </li> | ||
</div> | </div> | ||
</div> | </div> | ||
<div class='sub-nav__container'> | <div class='sub-nav__container'> | ||
| − | <li class='sub-nav__parent'> | + | <li id='a-notebook' class='sub-nav__parent'> |
| − | <a>Notebook</a> | + | <a> |
| + | Notebook | ||
| + | <div class='nav-container__arrow'></div> | ||
| + | </a> | ||
</li> | </li> | ||
<div class='sub-nav__children hidden'> | <div class='sub-nav__children hidden'> | ||
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</li> | </li> | ||
<li class='sub-nav__child'> | <li class='sub-nav__child'> | ||
| − | <a>Workflow</a> | + | <a href="https://2015.igem.org/Team:Northeastern_Boston/Workflow">Workflow</a> |
</li> | </li> | ||
</div> | </div> | ||
</div> | </div> | ||
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| − | |||
| − | |||
<div class='sub-nav__container'> | <div class='sub-nav__container'> | ||
| − | <li class='sub-nav__parent'> | + | <li id='a-humanpractices' class='sub-nav__parent'> |
| − | <a>Human Practices</a> | + | <a> |
| + | Human Practices | ||
| + | <div class='nav-container__arrow'></div> | ||
| + | </a> | ||
</li> | </li> | ||
<div class='sub-nav__children hidden'> | <div class='sub-nav__children hidden'> | ||
<li class='sub-nav__child'> | <li class='sub-nav__child'> | ||
| − | <a href="https://2015.igem.org/Team:Northeastern_Boston/ | + | <a href="https://2015.igem.org/Team:Northeastern_Boston/Collaborations">Collaborations</a> |
</li> | </li> | ||
<li class='sub-nav__child'> | <li class='sub-nav__child'> | ||
| − | <a href="https://2015.igem.org/Team:Northeastern_Boston/ | + | <a href="https://2015.igem.org/Team:Northeastern_Boston/Practices">Outreach</a> |
</li> | </li> | ||
<li class='sub-nav__child'> | <li class='sub-nav__child'> | ||
| − | <a href="https://2015.igem.org/Team:Northeastern_Boston/ | + | <a href="https://2015.igem.org/Team:Northeastern_Boston/Written_Investigation">Written Investigation</a> |
</li> | </li> | ||
</div> | </div> | ||
</div> | </div> | ||
<li> | <li> | ||
| − | <a href="https://2015.igem.org/Team:Northeastern_Boston/safety">Safety</a> | + | <a id="a-safety" href="https://2015.igem.org/Team:Northeastern_Boston/safety">Safety</a> |
</li> | </li> | ||
<li> | <li> | ||
| − | <a href="https://2015.igem.org/Team:Northeastern_Boston/measurement"> | + | <a id="a-measurement" href="https://2015.igem.org/Team:Northeastern_Boston/measurement">Interlab</a> |
</li> | </li> | ||
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<div id="project_description" class='article'> | <div id="project_description" class='article'> | ||
| − | <h1> | + | <h1 id="project_header" style="border-width:0">BASIC & COMPOSITE PARTS</h1> |
| + | |||
| + | <h2><a href="http://parts.igem.org/partsdb/edit_seq.cgi?part=BBa_K1857001" target="_blank" style="color:#7CAAD9; margin-right:25px">BBa_K1857001</a> Hygromycin B Resistance Cassette </h2> | ||
| + | |||
| + | <img src="https://static.igem.org/mediawiki/2015/0/0c/HygResCassettetake14-01.jpg" style="width:100%"> | ||
| − | < | + | <p>Recognizing that the iGEM repository lacks a modular selection marker for microalgae, Northeastern submits a Hygromycin B resistance cassette. This composite part was cloned from <a href="http://chlamycollection.org/plasmid/popt_mvenus_hyg" target="_blank">pOpt_mVenus_Hyg</a> and adapted to the iGEM standards. As a complete resistance cassette, this part is expected to confer Hygromycin B resistance to any <i>Chlamydomonas reinhardtii</i> transformed with this part.</p> |
| − | < | + | <p>This part contains intron 2 of RBCS2 after a fusion HSP70A-RBCS2 promoter. [<a href="http://onlinelibrary.wiley.com/store/10.1046/j.1365-313x.2000.00652.x/asset/j.1365-313x.2000.00652.x.pdf?v=1&t=iejf1jm1&s=f3a9125d087876eeeb8fe8892916ac1a9a96c3a9" target="_blank">1</a>] Aph7'', a phosphotransferase, confers resistance to Hygromycin B at agar antibiotic concentrations up to 200g/mL. [<a href="http://www.sciencedirect.com/science/article/pii/S1434461004701170" target="_blank">2</a>]</p> |
| − | < | + | <h2><a href="http://parts.igem.org/partsdb/edit_seq.cgi?part=BBa_K1857000" target="_blank" style="color:#7CAAD9; margin-right:25px">BBa_K1857000</a> mVenus Fluorescent Protein</h2> |
| − | < | + | <img src="https://static.igem.org/mediawiki/2015/c/ce/MVenusattempt11-01.jpg" style="width:100%"> |
| + | <p>This part contains mVenus, a nuclear codon-optimized YFP variant (excitation/emission: 515/528nm) [<a href="http://onlinelibrary.wiley.com/doi/10.1111/tpj.12165/full" target="_blank">3</a>]. It has Intron 1 and 2 of RBCS2 for increased expression. This is the first independent fluorescent protein codon-optimized for <i>Chlamydomonas reinhardtii</i> submitted to the iGEM registry.</p> | ||
Latest revision as of 23:01, 18 September 2015
BASIC & COMPOSITE PARTS
BBa_K1857001 Hygromycin B Resistance Cassette
Recognizing that the iGEM repository lacks a modular selection marker for microalgae, Northeastern submits a Hygromycin B resistance cassette. This composite part was cloned from pOpt_mVenus_Hyg and adapted to the iGEM standards. As a complete resistance cassette, this part is expected to confer Hygromycin B resistance to any Chlamydomonas reinhardtii transformed with this part.
This part contains intron 2 of RBCS2 after a fusion HSP70A-RBCS2 promoter. [1] Aph7'', a phosphotransferase, confers resistance to Hygromycin B at agar antibiotic concentrations up to 200g/mL. [2]
BBa_K1857000 mVenus Fluorescent Protein
This part contains mVenus, a nuclear codon-optimized YFP variant (excitation/emission: 515/528nm) [3]. It has Intron 1 and 2 of RBCS2 for increased expression. This is the first independent fluorescent protein codon-optimized for Chlamydomonas reinhardtii submitted to the iGEM registry.