Difference between revisions of "Team:Technion Israel/Parts"

 
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<a href="https://2015.igem.org/Team:Technion_Israel/Team/About_Us">About&nbsp;Us</a>
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<a href="https://2015.igem.org/Team:Technion_Israel/Team/About_Us" id="Team_Links_About_Us">About&nbsp;Us</a>
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<a href="https://2015.igem.org/Team:Technion_Israel/Parts">Parts</a>
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<a href="https://2015.igem.org/Team:Technion_Israel/Judging_Criteria/Achievements">Achievements</a>
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<a href="https://2015.igem.org/Team:Technion_Israel/Project/Overview">Overview</a>
 
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<a href="https://2015.igem.org/Team:Technion_Israel/Project/Bacillus">Bacillus</a>
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<a href="https://2015.igem.org/Team:Technion_Israel/Project/Secretion">Secretion</a>
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<a href="https://2015.igem.org/Team:Technion_Israel/Project/NADPH">NADPH</a>
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<br><h1>Parts</h1>
 
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<h1><b>Parts</b></h1>
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<h2><b>The Design</b></h2>
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<p>The <b>3α-HSD</b> is secreted from <i>B. subtilis</i> as a pre-protein fused to the <b>Secretion Signal Peptide</b>. At the same time, the <b>G6PD</b> is expressed in <i>E. coli</i> to overproduce NADPH, a cofactor of the 3α-HSD enzyme.</p>
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<p>Both bacteria are placed in a syringe with a membrane that prevents the exit of <i>E. coli</i> onto the user's scalp. The medium with the <i>B. subtilis</i> is transferred to the scalp by a special 3-D printed comb.</p>
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<p>On the scalp, the 3α-HSD catalyzes the reduction of DHT to 3α-androstanediol with NADPH from the <i>E. coli</i> as a cofactor.</p>
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<p>An illustration of this concept can be  seen in Figure 1 below.</p>
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<figure><img src="https://static.igem.org/mediawiki/2015/6/69/Technion_Israel_2015_parts.png" width="100%"/><figcaption>Figure 1: Schematic illustration of project design and relevant BioBrick parts</figcaption></figure></br>
 
<h2><b>Biobricks</b></h2>
 
<h2><b>Biobricks</b></h2>
 
<p>The table below features the parts which we submitted to the <a href="http://parts.igem.org/Main_Page" target="_blank">iGEM Registry</a> of Standard Biological Parts</a>.  Information about the applications and characterization of each part can be seen in the biobrick pages of the registry.
 
<p>The table below features the parts which we submitted to the <a href="http://parts.igem.org/Main_Page" target="_blank">iGEM Registry</a> of Standard Biological Parts</a>.  Information about the applications and characterization of each part can be seen in the biobrick pages of the registry.
 
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<div id='groupparts' style='min-height:100px;width:700px;'><div style='width:300px;margin:2px;padding:20px;color:gray;border:1px solid gray'>Loading.....</div></div><script>$('#groupparts').load('/cgi/api/groupparts.cgi', { t:'iGEM015', g:'Technion_Israel' },function(){ $('#groupparts .tablesorter').tablesorter();} );</script>
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<div id='groupparts' style='min-height:100px;width:700px;'><div style='width:300px;margin:2px;padding:20px;color:gray;border:1px solid gray'>Loading.....</div></div><script>$('#groupparts').load('/cgi/api/groupparts.cgi', { t:'iGEM015', g:'Technion_Israel' },function(){ $('#groupparts .tablesorter').tablesorter();} );</script></br>
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<h2><b>Our Favorite New Parts</b></h2>
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<p><b>Secretion Signal Peptide, <a href="http://parts.igem.org/Part:BBa_K1674000" target="_blank">BBa_K1674000</a>:</b> Originated from the <i>B.subtilis</i> extracellular protease AprE. This signal peptide is routed via the Sec export-dependent pathway.</p>
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<p><b>3α-Hydroxysteroid Dehydrogenase - 3α-HSD, <a href="http://parts.igem.org/Part:BBa_K1674001" target="_blank">BBa_K1674001</a>:</b> Specific dehydrogenase originated from rat liver. Catalyzes the conversion of dihydrotestosterone (DHT) to 3α-androstanediol.</p>
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<p><b>Glucose-6-Phosphate Dehydrogenase - G6PD (<i>zwf</i>), <a href="http://parts.igem.org/Part:BBa_K1674004" target="_blank">BBa_K1674004</a>:</b> G6PD is a key enzyme since it catalyzes the first step of the pentose-phosphate pathway, which is the main resource of NADPH in the cell. </p>
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<h2><b>Best Basic Part</b></h2>
 
<h2><b>Best Basic Part</b></h2>
 
<p>We believe that our biobrick: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1674000">BBa_K1674000</a> is a pretty great basic part.  Go to the page for this <a href="https://2015.igem.org/Team:Technion_Israel/Basic_Part" target="_blank">Basic Part</a> to learn more.</p>
 
<p>We believe that our biobrick: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1674000">BBa_K1674000</a> is a pretty great basic part.  Go to the page for this <a href="https://2015.igem.org/Team:Technion_Israel/Basic_Part" target="_blank">Basic Part</a> to learn more.</p>

Latest revision as of 23:04, 18 September 2015

Team: Technion 2015

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Parts

The Design

The 3α-HSD is secreted from B. subtilis as a pre-protein fused to the Secretion Signal Peptide. At the same time, the G6PD is expressed in E. coli to overproduce NADPH, a cofactor of the 3α-HSD enzyme.

Both bacteria are placed in a syringe with a membrane that prevents the exit of E. coli onto the user's scalp. The medium with the B. subtilis is transferred to the scalp by a special 3-D printed comb.

On the scalp, the 3α-HSD catalyzes the reduction of DHT to 3α-androstanediol with NADPH from the E. coli as a cofactor.

An illustration of this concept can be seen in Figure 1 below.

Figure 1: Schematic illustration of project design and relevant BioBrick parts

Biobricks

The table below features the parts which we submitted to the iGEM Registry of Standard Biological Parts. Information about the applications and characterization of each part can be seen in the biobrick pages of the registry.

Loading.....

Our Favorite New Parts

Secretion Signal Peptide, BBa_K1674000: Originated from the B.subtilis extracellular protease AprE. This signal peptide is routed via the Sec export-dependent pathway.

3α-Hydroxysteroid Dehydrogenase - 3α-HSD, BBa_K1674001: Specific dehydrogenase originated from rat liver. Catalyzes the conversion of dihydrotestosterone (DHT) to 3α-androstanediol.

Glucose-6-Phosphate Dehydrogenase - G6PD (zwf), BBa_K1674004: G6PD is a key enzyme since it catalyzes the first step of the pentose-phosphate pathway, which is the main resource of NADPH in the cell.

Best Basic Part

We believe that our biobrick: BBa_K1674000 is a pretty great basic part. Go to the page for this Basic Part to learn more.

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