Difference between revisions of "Team:Technion Israel/Parts"

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.title h1{ color: rgb(173, 201, 219); font-weight: bold; font-size: 100px; text-align: center; background: rgba(0, 0, 0, 0.8); font-family: sans-serif; font-style: Arial; height: 100px; text-shadow: black 6px 6px 6px; line-height: 80px;}
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<h2> Part Documentation</h2>
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<title>Team: Technion 2015</title>
  
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<script src="http://ajax.googleapis.com/ajax/libs/jquery/1.11.1/jquery.min.js"></script> <!-- 33 KB -->
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<link  href="http://cdnjs.cloudflare.com/ajax/libs/fotorama/4.5.2/fotorama.css" rel="stylesheet"> <!-- 3 KB -->
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<a href="https://2015.igem.org/Team:Technion_Israel" style="position:fixed; width: 170px; display:inline-block; text-decoration: none;" class="nav_home_link">
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<h4>Note</h4>
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<img src="https://static.igem.org/mediawiki/2015/c/c1/Team_Technion_Navbar_Home.png" alt="Home" id="nav_home" /><div class="home_text">Home</div></a>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<div class="main_buttons">
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<div id="project" class="main_button">Project</div>
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<div id="Lab_Notebook" class="main_button">Lab&nbsp;Notebook</div>
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<div id="Human_Practices" class="main_button">Human&nbsp;Practices</div>
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<div id="Judging_Criteria" class="main_button">Judging&nbsp;Criteria</div>
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<div id="Team" class="main_button">Team</div>
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<a href="https://2015.igem.org/Team:Technion_Israel/Team/About_Us" id="Team_Links_About_Us">About&nbsp;Us</a>
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<a href="https://2015.igem.org/Team:Technion_Israel/Attributions" id="Team_Links_Attributions">Attributions</a>
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<a href="https://2015.igem.org/Team:Technion_Israel/Team/Sponsors" id="Team_Links_Sponsors">Sponsors</a>
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<a href="https://2015.igem.org/Team:Technion_Israel/Team/gallery" id="Team_Links_gallery">Gallery</a>
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<div id="Judging_Criteria_Links" class="main_links">
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<a href="https://2015.igem.org/Team:Technion_Israel/Parts" id="Judging_Criteria_Links_Parts">Parts</a>
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<a href="https://2015.igem.org/Team:Technion_Israel/Judging_Criteria/Achievements" id="Judging_Criteria_Links_Achievements">Achievements</a>
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<a href="https://2015.igem.org/Team:Technion_Israel/Collaborations" id="Judging_Criteria_Links_Collaborations">Collaborations</a>
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<div id="Human_Practices_Links" class="main_links">
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<a href="https://2015.igem.org/Team:Technion_Israel/Practices">Practices</a>
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<a href="https://2015.igem.org/Team:Technion_Israel/Practices/safety">Safety</a>
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<div id="Lab_Notebook_Links" class="main_links">
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<a href="https://2015.igem.org/Team:Technion_Israel/Lab_Notebook/Overview">Overview</a>
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<a href="https://2015.igem.org/Team:Technion_Israel/Lab_Notebook/Protocols">Protocols</a>
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<div id="project_Links" class="main_links">
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<a href="https://2015.igem.org/Team:Technion_Israel/Project/Overview">Overview</a>
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<a href="https://2015.igem.org/Team:Technion_Israel/Project/Secretion">Secretion</a>
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<a href="https://2015.igem.org/Team:Technion_Israel/Project/Expression">Expression</a>
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<a href="https://2015.igem.org/Team:Technion_Israel/Project/Cofactor">Cofactor</a>
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<a href="https://2015.igem.org/Team:Technion_Israel/Design">Comb</a>
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<a href="https://2015.igem.org/Team:Technion_Israel/Modeling">Modeling</a>
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<a href="https://2015.igem.org/Team:Technion_Israel/Project/Results">Results</a>
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<a href="#" class="up_link" style="text-decoration: none;">
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    <div class="up_button_text" style="text-decoration: none;">UP</div>
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    <img src="https://static.igem.org/mediawiki/2015/f/f4/Technion_2015_Up_suit.png" alt="Scroll to top" />
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<div class="title">
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<h1><b>Parts</b></h1>
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<h4>Adding parts to the registry</h4>
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<div class="main-text">
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<h2><b>The Design</b></h2>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<p>The <b>3α-HSD</b> is secreted from <i>B. subtilis</i> as a pre-protein fused to the <b>Secretion Signal Peptide</b>. At the same time, the <b>G6PD</b> is expressed in <i>E. coli</i> to overproduce NADPH, a cofactor of the 3α-HSD enzyme.</p>
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<p>Both bacteria are placed in a syringe with a membrane that prevents the exit of <i>E. coli</i> onto the user's scalp. The medium with the <i>B. subtilis</i> is transferred to the scalp by a special 3-D printed comb.</p>
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<p>On the scalp, the 3α-HSD catalyzes the reduction of DHT to 3α-androstanediol with NADPH from the <i>E. coli</i> as a cofactor.</p>
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<p>An illustration of this concept can be  seen in Figure 1 below.</p>
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<figure><img src="https://static.igem.org/mediawiki/2015/6/69/Technion_Israel_2015_parts.png" width="100%"/><figcaption>Figure 1: Schematic illustration of project design and relevant BioBrick parts</figcaption></figure></br>
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<h2><b>Biobricks</b></h2>
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<p>The table below features the parts which we submitted to the <a href="http://parts.igem.org/Main_Page" target="_blank">iGEM Registry</a> of Standard Biological Parts</a>. Information about the applications and characterization of each part can be seen in the biobrick pages of the registry.
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<div id='groupparts' style='min-height:100px;width:700px;'><div style='width:300px;margin:2px;padding:20px;color:gray;border:1px solid gray'>Loading.....</div></div><script>$('#groupparts').load('/cgi/api/groupparts.cgi', { t:'iGEM015', g:'Technion_Israel' },function(){ $('#groupparts .tablesorter').tablesorter();} );</script></br>
  
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<h2><b>Our Favorite New Parts</b></h2>
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<p><b>Secretion Signal Peptide, <a href="http://parts.igem.org/Part:BBa_K1674000" target="_blank">BBa_K1674000</a>:</b> Originated from the <i>B.subtilis</i> extracellular protease AprE. This signal peptide is routed via the Sec export-dependent pathway.</p>
  
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<h4>What information do I need to start putting my parts on the Registry?</h4>
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<p><b>3α-Hydroxysteroid Dehydrogenase - 3α-HSD, <a href="http://parts.igem.org/Part:BBa_K1674001" target="_blank">BBa_K1674001</a>:</b> Specific dehydrogenase originated from rat liver. Catalyzes the conversion of dihydrotestosterone (DHT) to 3α-androstanediol.</p>
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<p>The information needed to initially create a part on the Registry is:</p>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<p><b>Glucose-6-Phosphate Dehydrogenase - G6PD (<i>zwf</i>), <a href="http://parts.igem.org/Part:BBa_K1674004" target="_blank">BBa_K1674004</a>:</b> G6PD is a key enzyme since it catalyzes the first step of the pentose-phosphate pathway, which is the main resource of NADPH in the cell. </p>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h2><b>Best Basic Part</b></h2>
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<p>We believe that our biobrick: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1674000">BBa_K1674000</a> is a pretty great basic part.  Go to the page for this <a href="https://2015.igem.org/Team:Technion_Israel/Basic_Part" target="_blank">Basic Part</a> to learn more.</p>
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<h3>Contact Us</h3>
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<nav class="connect_links">
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<div class="facebook_link">
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<a href="https://www.facebook.com/technioniGEM2015?pnref=story" target="_blank">
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<img src="https://static.igem.org/mediawiki/2015/0/06/Team_Technion_Footer_facebook.png" alt="Follow us on facebook" /></a>
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<div class="email_link">
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<a href="mailto:igem.technion2015@gmail.com">
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<img src="https://static.igem.org/mediawiki/2015/d/da/Team_Technion_Footer_gmail.png" alt="Send us an Email" /></a>
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</footer>
  
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<h4>Inspiration</h4>
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<script type="text/javascript" src="jquery-1.8.0.min.js"></script>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<script type="text/javascript" src="https://2015.igem.org/Template:Technion/navbar/javascript?
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<groupparts>iGEM015 Example</groupparts>
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Latest revision as of 23:04, 18 September 2015

Team: Technion 2015

Home
Home
Project
Lab Notebook
Human Practices
Judging Criteria
Team
UP
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Parts

The Design

The 3α-HSD is secreted from B. subtilis as a pre-protein fused to the Secretion Signal Peptide. At the same time, the G6PD is expressed in E. coli to overproduce NADPH, a cofactor of the 3α-HSD enzyme.

Both bacteria are placed in a syringe with a membrane that prevents the exit of E. coli onto the user's scalp. The medium with the B. subtilis is transferred to the scalp by a special 3-D printed comb.

On the scalp, the 3α-HSD catalyzes the reduction of DHT to 3α-androstanediol with NADPH from the E. coli as a cofactor.

An illustration of this concept can be seen in Figure 1 below.

Figure 1: Schematic illustration of project design and relevant BioBrick parts

Biobricks

The table below features the parts which we submitted to the iGEM Registry of Standard Biological Parts. Information about the applications and characterization of each part can be seen in the biobrick pages of the registry.

Loading.....

Our Favorite New Parts

Secretion Signal Peptide, BBa_K1674000: Originated from the B.subtilis extracellular protease AprE. This signal peptide is routed via the Sec export-dependent pathway.

3α-Hydroxysteroid Dehydrogenase - 3α-HSD, BBa_K1674001: Specific dehydrogenase originated from rat liver. Catalyzes the conversion of dihydrotestosterone (DHT) to 3α-androstanediol.

Glucose-6-Phosphate Dehydrogenase - G6PD (zwf), BBa_K1674004: G6PD is a key enzyme since it catalyzes the first step of the pentose-phosphate pathway, which is the main resource of NADPH in the cell.

Best Basic Part

We believe that our biobrick: BBa_K1674000 is a pretty great basic part. Go to the page for this Basic Part to learn more.

Contact Us