Difference between revisions of "Team:Tufts/Results"
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− | The first few steps of the project involved purifying and checking both of the genes for Cas9 and aTcdB. This involved purifying the products of PCR and running them on electrophoresis gels, as well as using a nanodrop machine to identify the concentration and purity of DNA recovered. | + | <p>The first few steps of the project involved purifying and checking both of the genes for Cas9 and aTcdB. This involved purifying the products of PCR and running them on electrophoresis gels, as well as using a nanodrop machine to identify the concentration and purity of DNA recovered.</p> |
− | pHis1522-aTcdB | + | 1. pHis1522-aTcdB<br> |
− | + | <table> | |
Sample | Sample | ||
Concentration (ng/μL) | Concentration (ng/μL) | ||
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B | B | ||
170.2 | 170.2 | ||
− | + | </table> | |
2. pHsp70-Cas9 | 2. pHsp70-Cas9 | ||
Revision as of 23:28, 18 September 2015
The first few steps of the project involved purifying and checking both of the genes for Cas9 and aTcdB. This involved purifying the products of PCR and running them on electrophoresis gels, as well as using a nanodrop machine to identify the concentration and purity of DNA recovered.
1. pHis1522-aTcdB![](https://static.igem.org/mediawiki/2015/3/3e/Tuftsresultsgel1.png)
![](https://static.igem.org/mediawiki/2015/8/86/Tuftsresultsgel2.png)