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| | <h2>Protocols</h2> | | <h2>Protocols</h2> |
| − | <h3><a href="#">Preparing liquid YEPD media</a></h3> | + | <h3><a href="https://2015.igem.org/Team:London_Biohackspace/protocols/liiquid-yepd">Preparing liquid YEPD media</a></h3> |
| − | <p>1. For 1 litre of YEPD media measure the following into an erlenmayer or duran flask<br/><br/> | + | <h3><a href="https://2015.igem.org/Team:London_Biohackspace/protocols/yepd-agar-plates">Preparing YEPD agar plates</a></h3> |
| − | Bacto yeast extract (Difco 0127-17) (1%) 10g<br/>
| + | <h3><a href="https://2015.igem.org/Team:London_Biohackspace/protocols/ecoli-tansformation">Electroporation Transformation of <i>E. coli</i> DH5aplha</a></h3> |
| − | Bacto peptone (Difco 0118-17) (2%) 20g<br/>
| + | < h3><a href=" https:// 2015.igem.org/ Team:London_Biohackspace/ protocols/yeast-tansformation"> Electroporation Transformation of <i>S. cerevisiae</i></a></h3> |
| − | Glucose (2%) 20g<br/><br/>
| + | |
| − | Available premixed as Difco 0428-17.<br/><br/>
| + | |
| − | 2. Autoclave for at least 15 minutes at 121C<br/>
| + | |
| − | </p>
| + | |
| − | <h3>Preparing YEPD agar plates</h3>
| + | |
| − | <p>1. For 1 litre of YEPD media measure the following into an erlenmayer or duran flask<br/><br/>
| + | |
| − | Bacto yeast extract (Difco 0127-17) (1%) 10g<br/> | + | |
| − | Bacto peptone (Difco 0118-17) (2%) 20g<br/>
| + | |
| − | Glucose (2%) 20g<br/><br/>
| + | |
| − | Available premixed as Difco 0428-17.<br/><br/>
| + | |
| − | Bacto agar (Difco 0140-01) (2%) 20g<br/><br/>
| + | |
| − | 2. Autoclave for at least 15 minutes at 121C<br/>
| + | |
| − | 2. Pour 20ml into an agar plate. Leave to cool at room temperature for 2-3 hours and store at -4C<br/>
| + | |
| − | </p>
| + | |
| − | <h3>Electroporation Transformation of <i>E. coli</i> DH5aplha</h3>
| + | |
| − | <p><ul> | + | |
| − | <li><a href=" #">Protocol</ a></ li> | + | |
| − | </ul></p>
| + | |
| − | <h3><a href="#">Transformation of <i>S. cerevisiae</i></a></h3>
| + | |
| − | <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</p>
| + | |
| | | | |
| | <h2>Experiments</h2> | | <h2>Experiments</h2> |
| − | <h3>Expressing Miraculin in <i>S. cerevisiae</i></h3> | + | <h3><a href="https://2015.igem.org/Team:London_Biohackspace/protocols/egassembly">SLiCE/<i>Ex vivo</i> DNA Assembly <i> i.e.</i> Cheap Gibson </a></h3> |
| − | <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</p>
| + | <h3><a href="https://2015.igem.org/Team:London_Biohackspace/protocols/filtercomp">Filtration method for competent cells preparation</a></h3> |
| − | <h3>Expressing Lycopene in <i>S. cerevisiae</i></h3>
| + | <h3><a href="https://2015.igem.org/Team:London_Biohackspace/experiments/yeast-culturing">CULTURING 'WILD' <i>S. cerevisiae</i></a></h3> |
| − | <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</p> | + | <h3><a href="https://2015.igem.org/Team:London_Biohackspace/experiments/miraculin">Expressing Miraculin in <i>S. cerevisiae</i></a></h3> |
| − | <h3>Creating Leucine and Uracil auxotrophic <i>S. cerevisiae</i> strains</h3>
| + | <h3><a href="https://2015.igem.org/Team:London_Biohackspace/experiments/rna-based-regulation">RNA interference based regulation of RFP in <i>E. coli</i></a></h3> |
| − | <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</p> | + | <h3><a href="https://2015.igem.org/Team:London_Biohackspace/experiments/lycopene">Expressing Lycopene in <i>E.coli</i> and <i>S. cerevisiae</i></a></h3> |
| − | <h3>RNAi based regulation of mRFP in <i>E. coli</i></h3>
| + | <h3><a href="https://2015.igem.org/Team:London_Biohackspace/experiments/aking-auxotrophic-yeast">Creating Leucine auxotrophic <i>S. cerevisiae</i> strains</a></h3> |
| − | <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</p> | + | |
| − | | + | |
| − | <h2>Results</h2>
| + | |
| − | <h3>Expressing Miraculin in <i>S. cerevisiae</i></h3>
| + | |
| − | <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</p> | + | |
| − | <h3>Expressing Lycopene in <i>S. cerevisiae</i></h3>
| + | |
| − | <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</p> | + | |
| − | <h3>Creating Leucine and Uracil auxotrophic <i>S. cerevisiae</i> strains</h3>
| + | |
| − | <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</p> | + | |
| − | <h3>RNAi based regulation of mRFP in <i>E. coli</i></h3>
| + | |
| − | <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</p>
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| − | | + | |
| | </div> | | </div> |
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