Difference between revisions of "Team:SZU China/Safety"

 
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    <p id="heading">Safety</p>
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            <h2>About Our Lab</h2>
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            <h3>1.Chassis and Organism</h3>
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            <p>The chassis organism is human bladder cancer cell line 5637 and T24.The organism used for control group are Hela（cervical carcinoma cell) and HFC (human fiber epithelial cell).</p>
  
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            <h2>Experiment environment</h2>
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            <p>We handle the biological material in Ultra clean bench and Cell Culture Laboratory. For protective equipment, for example, we use a board to protect ourselves from the X-ray when we are doing gel extraction under the gel imager.</p>
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            <h2>Rules and Regulations on safety</h2>
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            <h3>1.Link to the laboratory safety training requirements of our institution</h3>
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            <p>The college of Biology and Life Science has clear regulation about the safety of biology labs including the pre-training courses on safety manipulation before experiment in the labs,docunmenting record in the labs etc.We have Laboratory And Equipment Department at our institution which is responsible for our biological safety.The officers have inspected our laboratory and gave us suggestions about biosafety, which were already fulfilled as request.
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<a href="http://sbc.szu.edu.cn/2013/key.asp?trees=:0:97:124:&ID=485">Regulations of laboratory safety and environmental management of Shenzhen University</a></p>
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            <h3>2.Regulations that govern biosafety in research laboratories</h3>
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            <p><a href="http://www.biosafety.gov.cn/gjzcfg/flfg/200401/t20040115_88044.htm">Safety management method for gene engineering in China</a></p>
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            <h2>Risk of Project</h2>
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            <h3>1.Potential risk and solutions</h3>
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            <p>If the experiment did not proceed carefully that result in the leak of the culture medium, the bacteria may breed at a considerable speed especially for the human cell culture medium and serum we would use in our lab.Thus it will contaminate the lab.To prevent this,we instruct every student to learn the lab regulations and they are asked to check the vessels and dispose the waste well before exiting from the lab. Referring to the case that people carry the extraneous bacteria into the lab without disinfection, we have strict hygiene standard which requires all entrants to wear the lab coat and disinfect before experiment.</p>
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            <h3>2.future work and risk solutions</h3>
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            <p>If the system develops well in the future,it could be injected into human body to cure the bladder cancer. When doing so,though our system won't hurt normal cells,the translation of effector gene before termination codon may cause burden to normal cells.To reduce these risk, when we designed the mutation site,we choose the site as far as possible close to the front.</p>
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<a href="https://2015.igem.org/Team:SZU_China"><li>HOME</li></a>
 
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<a href="https://2015.igem.org/Team:SZU_China/Team"><li>TEAM</li></a>
 
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<a href="#"><li>PROJECT
 
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<a href="https://2015.igem.org/Team:SZU_China/Description"><li>Description</li></a>
 
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<a href="https://2015.igem.org/Team:SZU_China/Experiments"><li>Experiments & Protocols</li></a> 
 
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<a href="https://2015.igem.org/Team:SZU_China/Results"><li>Results</li></a> 
 
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<a href="#"><li>PARTS
 
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<a href="https://2015.igem.org/Team:SZU_China/Basic_Part"><li>Basic Parts</li></a> 
 
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<a href="https://2015.igem.org/Team:SZU_China/Composite_Part"><li>Composite Parts</li></a>
 
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<a href="https://2015.igem.org/Team:SZU_China/Part_Collection"><li>Part Collection</li></a> 
 
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<a href="https://2015.igem.org/Team:SZU_China/Practices"><li>HUMAN PRACTICES</li></a>
 
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<a href="https://2015.igem.org/Team:SZU_China/Safety"><li>SAFETY</li></a>
 
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<a href="https://2015.igem.org/Team:SZU_China/Modeling"><li>MODELING</li></a>
 
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<a href="https://2015.igem.org/Team:SZU_China/Measurement"><li>MEASUREMENT</li></a>
 
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<a href="https://2015.igem.org/Team:SZU_China/Software"><li>SOFTWARE</li></a>
 
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<h1> WIKI 正在建设中 </h1>
 
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<h2>Safety in iGEM</h2>
 
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<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
 
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<h4>Safe Project Design</h4>
 
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
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<li>Choosing a non-pathogenic chassis</li>
 
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<li>Choosing parts that will not harm humans / animals / plants</li>
 
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
 
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<li>Including an "induced lethality" or "kill-switch" device</li>
 
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<h4>Safe Lab Work</h4>
 
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
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<h4>Safe Shipment</h4>
 
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
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Latest revision as of 00:02, 19 September 2015



Safety




About Our Lab

1.Chassis and Organism

The chassis organism is human bladder cancer cell line 5637 and T24.The organism used for control group are Hela（cervical carcinoma cell) and HFC (human fiber epithelial cell).

Experiment environment

We handle the biological material in Ultra clean bench and Cell Culture Laboratory. For protective equipment, for example, we use a board to protect ourselves from the X-ray when we are doing gel extraction under the gel imager.

Rules and Regulations on safety

1.Link to the laboratory safety training requirements of our institution

The college of Biology and Life Science has clear regulation about the safety of biology labs including the pre-training courses on safety manipulation before experiment in the labs,docunmenting record in the labs etc.We have Laboratory And Equipment Department at our institution which is responsible for our biological safety.The officers have inspected our laboratory and gave us suggestions about biosafety, which were already fulfilled as request. Regulations of laboratory safety and environmental management of Shenzhen University


2.Regulations that govern biosafety in research laboratories

Safety management method for gene engineering in China

Risk of Project

1.Potential risk and solutions

If the experiment did not proceed carefully that result in the leak of the culture medium, the bacteria may breed at a considerable speed especially for the human cell culture medium and serum we would use in our lab.Thus it will contaminate the lab.To prevent this,we instruct every student to learn the lab regulations and they are asked to check the vessels and dispose the waste well before exiting from the lab. Referring to the case that people carry the extraneous bacteria into the lab without disinfection, we have strict hygiene standard which requires all entrants to wear the lab coat and disinfect before experiment.


2.future work and risk solutions

If the system develops well in the future,it could be injected into human body to cure the bladder cancer. When doing so,though our system won't hurt normal cells,the translation of effector gene before termination codon may cause burden to normal cells.To reduce these risk, when we designed the mutation site,we choose the site as far as possible close to the front.