Difference between revisions of "Team:Lethbridge/Composite Part"

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<h2> Composite Parts</h2>
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<h4>Note</h4>
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<p>In order to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best New Composite Part award</a>, you must fill out this page. Please give links to the Registry entries for the Composite parts you have made. Please see the Registry's <a href="http://parts.igem.org/Help:Parts#Basic_and_Composite_Parts"> Help:Parts page</a> for more information on part types.</p>
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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                <ul>
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                    <li><a href="https://2015.igem.org/">iGEM</a></li>
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                    <li><a href="https://2015.igem.org/Team:Lethbridge">RNAiCare</a></li>
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                    <li>
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                        <a href="https://2015.igem.org/Team:Lethbridge/Project">Project</a>
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                        <ul>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Description">Description</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Design">Design</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Results">Results</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Project_Production">Production</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Project_Judging">Judging</a></li>
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                        </ul>
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                    </li>
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                    <li>
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                        <a href="https://2015.igem.org/Team:Lethbridge/Parts">Parts</a>
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                        <ul>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Basic_Part">Basic Parts</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Composite_Part">Composite Parts</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Measurement">Measurement</a></li>
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                        </ul>
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                        <a href="https://2015.igem.org/Team:Lethbridge/Practices">Practices</a>
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                        <ul>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Practices_Risks">Risks</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Practices_Stakeholders">Stakeholders</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Practices_Current">Current Problems</a></li>
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                        </ul>
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                    </li>
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                    <li>
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                        <a href="https://2015.igem.org/Team:Lethbridge/Notebook">Notebook</a>
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                        <ul>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Notebook_July">July</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Notebook_August">August</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Notebook_September">September</a></li>
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                        </ul>
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                        <a href="https://2015.igem.org/Team:Lethbridge/Team">Team</a>
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                        <ul>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Team_Members">Members</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Collaborations">Collaborations</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Attributions">Attributions</a></li>
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                            <li><a href="https://2015.igem.org/Team:Lethbridge/Sponsors">Sponsors</a></li>
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                        </ul>
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                    </li>
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                    <h1>Composite Parts</h1>
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                    <h2>Part BBa_K1791001</h2>
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<p>Part BBa_K1791001 contains two modules, a standard ribosome binding site (RBS) expressing MS2 coat protein and a theophylline ribozyme with a MS2 aptamer at the 3’ end. To express MS2 coat  protein the part utilizes expression of c-terminal 6x His tagged MS2 coat protein under the regulation of  a T7 promoter and a standard Shine Dalgarno sequence followed by a double terminator (BBa_0015).  Downstream of the RBS is a T7 promoter followed by a theophylline ribozyme and a MS2 aptamer at the  3’ end. Coexpression of the two modules results in MS2 binding to the 3’ end of the theophylline ribozyme module which allows for MS2-RNA purification by nickel affinity chromatography, RNA can  then be eluted from the MS2-RNA complex by addition of theophylline.  </p>
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<img src="https://static.igem.org/mediawiki/2015/8/84/Uleth15_StandardRPA.png" height="30%" width="30%">
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<p>Check it out on the <a href="http://parts.igem.org/Part:BBa_K1791001">Registry</a></p>
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                    <h2>Part BBa_K1791002</h2>
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<p>Part BBa_K17910012 contains two modules, a low efficiency ribosome binding site (RBS)  expressing MS2 coat protein and a theophylline ribozyme with a MS2 aptamer at the 3’ end. To express  MS2 coat protein the part utilizes expression of c-terminal 6x His tagged MS2 coat protein under a T7  promoter and a standard Shine Dalgarno sequence followed by a double terminator (BBa_0015).  Downstream of the RBS is a T7 promoter followed by a theophylline ribozyme and a MS2 aptamer at the  3’ end. Coexpression of the two modules results in MS2 binding to the 3’ end of the theophylline  ribozyme construct which allows for MS2-RNA purification by nickel affinity chromatography, RNA can  then be eluted from the MS2-RNA complex by addition of theophylline.</p>
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<img src="https://static.igem.org/mediawiki/2015/e/e5/Uleth_ConstructSchematic.png" height="30%" width="30%">
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<p>Check it out on the <a href="http://parts.igem.org/Part:BBa_K1791002">Registry</a></p>
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Latest revision as of 00:37, 19 September 2015

iGEM

Composite Parts

Part BBa_K1791001

Part BBa_K1791001 contains two modules, a standard ribosome binding site (RBS) expressing MS2 coat protein and a theophylline ribozyme with a MS2 aptamer at the 3’ end. To express MS2 coat protein the part utilizes expression of c-terminal 6x His tagged MS2 coat protein under the regulation of a T7 promoter and a standard Shine Dalgarno sequence followed by a double terminator (BBa_0015). Downstream of the RBS is a T7 promoter followed by a theophylline ribozyme and a MS2 aptamer at the 3’ end. Coexpression of the two modules results in MS2 binding to the 3’ end of the theophylline ribozyme module which allows for MS2-RNA purification by nickel affinity chromatography, RNA can then be eluted from the MS2-RNA complex by addition of theophylline.

Check it out on the Registry

Part BBa_K1791002

Part BBa_K17910012 contains two modules, a low efficiency ribosome binding site (RBS) expressing MS2 coat protein and a theophylline ribozyme with a MS2 aptamer at the 3’ end. To express MS2 coat protein the part utilizes expression of c-terminal 6x His tagged MS2 coat protein under a T7 promoter and a standard Shine Dalgarno sequence followed by a double terminator (BBa_0015). Downstream of the RBS is a T7 promoter followed by a theophylline ribozyme and a MS2 aptamer at the 3’ end. Coexpression of the two modules results in MS2 binding to the 3’ end of the theophylline ribozyme construct which allows for MS2-RNA purification by nickel affinity chromatography, RNA can then be eluted from the MS2-RNA complex by addition of theophylline.

Check it out on the Registry