Difference between revisions of "Team:Lethbridge/Composite Part"
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+ | <ul> | ||
+ | <li><a href="https://2015.igem.org/">iGEM</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Lethbridge">RNAiCare</a></li> | ||
+ | <li> | ||
+ | <a href="https://2015.igem.org/Team:Lethbridge/Project">Project</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2015.igem.org/Team:Lethbridge/Description">Description</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Lethbridge/Design">Design</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Lethbridge/Results">Results</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Lethbridge/Project_Production">Production</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Lethbridge/Project_Judging">Judging</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2015.igem.org/Team:Lethbridge/Parts">Parts</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2015.igem.org/Team:Lethbridge/Basic_Part">Basic Parts</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Lethbridge/Composite_Part">Composite Parts</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Lethbridge/Measurement">Measurement</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2015.igem.org/Team:Lethbridge/Practices">Practices</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2015.igem.org/Team:Lethbridge/Practices_Risks">Risks</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Lethbridge/Practices_Stakeholders">Stakeholders</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Lethbridge/Practices_Current">Current Problems</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2015.igem.org/Team:Lethbridge/Notebook">Notebook</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2015.igem.org/Team:Lethbridge/Notebook_July">July</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Lethbridge/Notebook_August">August</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Lethbridge/Notebook_September">September</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2015.igem.org/Team:Lethbridge/Team">Team</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2015.igem.org/Team:Lethbridge/Team_Members">Members</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Lethbridge/Collaborations">Collaborations</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Lethbridge/Attributions">Attributions</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Lethbridge/Sponsors">Sponsors</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | </ul> | ||
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+ | <h1>Composite Parts</h1> | ||
+ | |||
+ | <h2>Part BBa_K1791001</h2> | ||
+ | <p>Part BBa_K1791001 contains two modules, a standard ribosome binding site (RBS) expressing MS2 coat protein and a theophylline ribozyme with a MS2 aptamer at the 3’ end. To express MS2 coat protein the part utilizes expression of c-terminal 6x His tagged MS2 coat protein under the regulation of a T7 promoter and a standard Shine Dalgarno sequence followed by a double terminator (BBa_0015). Downstream of the RBS is a T7 promoter followed by a theophylline ribozyme and a MS2 aptamer at the 3’ end. Coexpression of the two modules results in MS2 binding to the 3’ end of the theophylline ribozyme module which allows for MS2-RNA purification by nickel affinity chromatography, RNA can then be eluted from the MS2-RNA complex by addition of theophylline. </p> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/8/84/Uleth15_StandardRPA.png" height="30%" width="30%"> | ||
+ | <p>Check it out on the <a href="http://parts.igem.org/Part:BBa_K1791001">Registry</a></p> | ||
+ | |||
+ | <h2>Part BBa_K1791002</h2> | ||
+ | <p>Part BBa_K17910012 contains two modules, a low efficiency ribosome binding site (RBS) expressing MS2 coat protein and a theophylline ribozyme with a MS2 aptamer at the 3’ end. To express MS2 coat protein the part utilizes expression of c-terminal 6x His tagged MS2 coat protein under a T7 promoter and a standard Shine Dalgarno sequence followed by a double terminator (BBa_0015). Downstream of the RBS is a T7 promoter followed by a theophylline ribozyme and a MS2 aptamer at the 3’ end. Coexpression of the two modules results in MS2 binding to the 3’ end of the theophylline ribozyme construct which allows for MS2-RNA purification by nickel affinity chromatography, RNA can then be eluted from the MS2-RNA complex by addition of theophylline.</p> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/e/e5/Uleth_ConstructSchematic.png" height="30%" width="30%"> | ||
+ | <p>Check it out on the <a href="http://parts.igem.org/Part:BBa_K1791002">Registry</a></p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
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Latest revision as of 00:37, 19 September 2015
Composite Parts
Part BBa_K1791001
Part BBa_K1791001 contains two modules, a standard ribosome binding site (RBS) expressing MS2 coat protein and a theophylline ribozyme with a MS2 aptamer at the 3’ end. To express MS2 coat protein the part utilizes expression of c-terminal 6x His tagged MS2 coat protein under the regulation of a T7 promoter and a standard Shine Dalgarno sequence followed by a double terminator (BBa_0015). Downstream of the RBS is a T7 promoter followed by a theophylline ribozyme and a MS2 aptamer at the 3’ end. Coexpression of the two modules results in MS2 binding to the 3’ end of the theophylline ribozyme module which allows for MS2-RNA purification by nickel affinity chromatography, RNA can then be eluted from the MS2-RNA complex by addition of theophylline.
![](https://static.igem.org/mediawiki/2015/8/84/Uleth15_StandardRPA.png)
Check it out on the Registry
Part BBa_K1791002
Part BBa_K17910012 contains two modules, a low efficiency ribosome binding site (RBS) expressing MS2 coat protein and a theophylline ribozyme with a MS2 aptamer at the 3’ end. To express MS2 coat protein the part utilizes expression of c-terminal 6x His tagged MS2 coat protein under a T7 promoter and a standard Shine Dalgarno sequence followed by a double terminator (BBa_0015). Downstream of the RBS is a T7 promoter followed by a theophylline ribozyme and a MS2 aptamer at the 3’ end. Coexpression of the two modules results in MS2 binding to the 3’ end of the theophylline ribozyme construct which allows for MS2-RNA purification by nickel affinity chromatography, RNA can then be eluted from the MS2-RNA complex by addition of theophylline.
![](https://static.igem.org/mediawiki/2015/e/e5/Uleth_ConstructSchematic.png)
Check it out on the Registry