Difference between revisions of "Team:Harvard BioDesign/Safety"

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{{Harvard_BioDesign}}
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<h2>Safety in iGEM</h2>
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              <span class="harvardLogo"><a href="https://2015.igem.org/Team:Harvard_BioDesign"><img src="https://static.igem.org/mediawiki/2015/9/94/HarvardBioDesign2015Logo2.png"alt="Harvard Logo" style="width:275px;height:200px;margin-left:-50px"/></a> </span>
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            <!-- <h1 id="title">Harvard BioDesign 2015</h1>-->
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                <li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Safety#project" id="top-link" class="skel-layers-ignoreHref"><span class="icon fa-home">Project Design</span></a></li>
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                <li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Safety#lab" id="overview-link" class="skel-layers-ignoreHref"><span class="icon fa-th">Safe Lab Work</span></a></li>
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                <li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Safety#shipment" id="sitemap-link" class="skel-layers-ignoreHref"><span class="icon fa-user">Safe Shipment</span></a></li>
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<li><a href="https://2015.igem.org/Team:Harvard_BioDesign">Home</a></li>
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<li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Project">Project</a></li>
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<li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Results">Results</a></li>
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<li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Notebook">Notebook</a></li>
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<li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Parts">Parts</a></li>
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<li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Practices">Practices</a></li>
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<li><a class="curPage" href="https://2015.igem.org/Team:Harvard_BioDesign/Safety">Safety</a></li>
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<li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Collaborations">Collaborations</a></li>
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<li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Team">Team</a></li> 
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<li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Attributions">Attributions</a></li>
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<img src = "https://static.igem.org/mediawiki/2015/4/44/Harvard2015_Safety.png"/>
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                <h2 class="alt">Project Design</h2>
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                <p>We extracted fim from the genome of E. coli K-12, a non-pathogenic laboratory strain. We transformed our plasmids into the Keio parent strain, another a non-pathogenic laboratory strain. In response to feedback from experts, we also transformed our plasmids into Nissle, a probiotic that is not only non-pathogenic but also beneficial. We knocked out mannose-mediated binding in some of our plasmids which makes our system safer because it prevents our engineered E. coli from binding to healthy epithelial cells.</p>
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                <h2>Safe Lab Work</h2>
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<p>We worked at the Joshi Lab in the Wyss Institute, a BSL-1 laboratory. Before beginning work in lab, we took a safety course online that trained us in general lab safety, biosafety, radioactive materials safety, and laser microscope use. We learned some important lab practices, such as wearing gloves, tying back long hair, and what to do in emergency situations, and also received a healthy dose of fear- to quote our training manual, “Blindness is forever!”.  One case where we had to take extra safety precautions was when we gel extracted bands from electrophoresis gels. We wore UV protection goggles to protect our eyes from the UV light.</p>
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<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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          <section id="shipment" class="three">
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            <div class="container">
  
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              <header>
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                <h2>Safe Shipment</h2>
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<p>Since we are based in Cambridge, close to iGEM HQ, we were able to skip the hassle of shipping and deliver our biobricks directly to iGEM HQ.</p>
  
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<h4>Safe Project Design</h4>
 
  
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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            </div>
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          </section>
  
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<ul>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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</ul>
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<h4>Safe Lab Work</h4>
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      </div>
  
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h4>Safe Shipment</h4>
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        <!-- Copyright -->
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          <li>Site template designed by: <a href="http://html5up.net">HTML5 UP</a></li>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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Latest revision as of 00:52, 19 September 2015


Prologue by HTML5 UP

Project Design

We extracted fim from the genome of E. coli K-12, a non-pathogenic laboratory strain. We transformed our plasmids into the Keio parent strain, another a non-pathogenic laboratory strain. In response to feedback from experts, we also transformed our plasmids into Nissle, a probiotic that is not only non-pathogenic but also beneficial. We knocked out mannose-mediated binding in some of our plasmids which makes our system safer because it prevents our engineered E. coli from binding to healthy epithelial cells.

Safe Lab Work

We worked at the Joshi Lab in the Wyss Institute, a BSL-1 laboratory. Before beginning work in lab, we took a safety course online that trained us in general lab safety, biosafety, radioactive materials safety, and laser microscope use. We learned some important lab practices, such as wearing gloves, tying back long hair, and what to do in emergency situations, and also received a healthy dose of fear- to quote our training manual, “Blindness is forever!”. One case where we had to take extra safety precautions was when we gel extracted bands from electrophoresis gels. We wore UV protection goggles to protect our eyes from the UV light.

Safe Shipment

Since we are based in Cambridge, close to iGEM HQ, we were able to skip the hassle of shipping and deliver our biobricks directly to iGEM HQ.