Difference between revisions of "Team:ETH Zurich/Safety"

 
(33 intermediate revisions by 4 users not shown)
Line 1: Line 1:
 
{{ETH_Zurich}}
 
{{ETH_Zurich}}
 
<html>
 
<html>
−
<h2>Safety at the ETH Zurich labs</h2>
+
<!-- Start of content -->
 +
<div class="expContainer" style=position:relative> <!--The closing tag for contentContainer should be placed at the bottom of each content page.-->
  
−
<p>Every new person in the lab must undergo a specifical safety training provided by Niels Bürckert.</p>
+
<h1>General Safety Considerations</h1>
  
−
<h3>Lab personal equipment</h3>
+
<!--[if gte IE 9]><!-->
−
<p>Lab coat use is compulsory inside any of the labs. This is essential for the proper protection of ourselves in case of splashing of chemicals. In our lab, the lab coats are colour coded: a green collar is for the normal lab, a yellow collar is for working with radioactivity, and the red collar is for the biosafety 2 lab. Of course, no lab cost can be worn inside the dining room!
+
<!--<div class="imgBox">-->
−
Goggles are essential for avoiding the entrance of chemicals inside the eyes. It is highly recommended to wear goggles all the time in the lab, as accidents may involve people who are nearby. Goggles have to protect the eyes, there is no use of them if they are hanging on the nose!</p>
+
<a href="https://static.igem.org/mediawiki/2015/6/6d/ETH_Safety.svg">
−
<p>Gloves is the third essential in the personal equipment. Gloves have to be used only once and then removed carefully to avoid any contact of contaminants with the skin. No gloves can be worn outside the lab, as small traces of chemicals might be on them!</p>
+
<object class="svg" data="https://static.igem.org/mediawiki/2015/6/6d/ETH_Safety.svg" width="12%" style="position:absolute;top:100px;right:44%">
 +
</object></a>
 +
<!--</div>-->
 +
<!--<![endif]-->
 +
<!--[if lte IE 8]>
 +
<![endif]-->
 +
<p></p>
 +
<p></p>
 +
<p></p>
  
−
<h3>General lab rules</h3>
+
<p></p>
−
<p>No smoking, eating or drinking is allowed inside the lab. A proper labelling of the chemicals has to be done. Nobody wants to find tens of suspiciously looking bottles after coming from the weekend!</p>
+
<p></p>
−
<p>Doors should usually be kept closed. This allow a proper exchange rate of air, can permit maintaining negative pressure in the labs that require it, and might be helpful in case of fire.
+
<p></p>
−
Chemicals have to be stored in an ordered way, never above the eye level and not in the middle of the way!</p>
+
<p></p>
 +
<p></p>
 +
<p></p>
  
−
<h3>Use of specific machinery</h3>
+
 
 +
 
 +
<h2>Method of Safety Evaluation</h2>
 +
 
 +
<p>To manage the risks associated with the development of our MicroBeacon CTC detection system we used the following scheme to evaluate possible adverse scenarios and the effectiveness of countermeasures:</p>
 +
 
 +
<img src="https://static.igem.org/mediawiki/2015/thumb/a/aa/RiskManagement.png/738px-RiskManagement.png" width="50%" align="center">
 +
 
 +
<p>Preventive measures can either serve to decrease the likelihood (x-axis) or the severity (y-axis) of a potential scenario or both. The goal is to move the risks as far as possible into the green corner.</p>
 +
 
 +
 
 +
 
 +
 
 +
<h2>Working at the ETH Zurich Labs</h2>
 +
 
 +
<p>Responsible conduct during everyday lab hours is parmount for the prevention of accidents. Every new person in the ETH D-BSSE lab must undergo <a href="https://www.bsse.ethz.ch/department/administration-services/infrastructure/safety-security.html">safety training instructed by a Technical Manager</a>. No exception was made for our iGEM team. Though much of it is common sense and our work was restricted to Biosafety Level 1, there is still a lot that can go wrong. Here are a few examples:</p>
 +
 
 +
<table>
 +
<tr>
 +
<td><b>Possible Scenario</b></td>
 +
<td><b>Old Risk</b></td>
 +
<td><b>Countermeasures</b></td>
 +
<td><b>New Risk</b></td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td><b>Accidental poisoning</b></td>
 +
<td>
 +
<img src="https://static.igem.org/mediawiki/2015/7/71/RiskE4.png" width="70%">
 +
</td>
 +
<td>
 
<ul>
 
<ul>
−
<li><p><i>Centrifuge</i>: must be always properly equilibrated. An unequilibrated centrifuge can be lethal if it dismantles at high speed.</p></li>
+
<li><p>Wear lab coats and safety goggles at all times</p>
−
<li><p><i>Balance</i>: must be kept clean at all times! No one wants to find a suspicious unknown powder spread all over the place.</p></li>
+
</li>
−
</ul>
+
<li><p>Wear gloves, be concentrated and handle chemicals with extra care.</p>
 +
</li>
 +
<li><p>No smoking, eating or drinking is in the lab.</p>
 +
</li>
 +
<li><p>Chemicals have to be properly labelled.</p>
 +
</li>
 +
<li><p>Just in case save emergency numbers on your phone: Ambulance (144) and Center for Toxicology (145)</p>
 +
</li>
 +
</b></td>
 +
<td>
 +
<img src="https://static.igem.org/mediawiki/2015/d/d5/RiskD1.png" width="70%">
 +
</td>
 +
</tr>
  
−
<h3>What to do if an accident occurs?</h3>
+
 
 +
<tr>
 +
<td><b>My <i>E. coli</i> are on fire!</b></td>
 +
<td>
 +
<img src="https://static.igem.org/mediawiki/2015/7/71/RiskE4.png" width="70%">
 +
</td>
 +
<td>
 
<ul>
 
<ul>
−
<li><i>All the bottle of ethidium bromide ended up on my lab coat!</i>You need to go outside the lab and take an emergency shower. It is better if you also take out the potentially contaminated clothes.
+
<p>Since we use Bunsen burners to create a semi-sterile working environment it is quite likely that something catches fire.
−
<li><i>I forgot to put my goggles on and now I have sulphuric acid in my eye! </i>There are eye washers in every lab. The eye should be washed for about ten minutes and a doctor must be visited afterwards to check the state of the eye and if it suffered any damage.</li>
+
<li><p>Keep flamable items (paper, cloth, flamable chemicals) away from the flame.</p>
−
<li><i>My</i> E. coli <i>are on fire</i>! First, do not panic! By panicking one might expand the fire. If the fire is small, it is possible to use a fire blanket to cut the access of oxygen to the fire. If the fire is difficult to isolate or if it involves the presence of chemicals it is better to use a CO2 fire extinguisher. Also, emergency services have to be called just in case the fire gets out of control.</li>
+
</li>
−
</ul>
+
<li><p>Have a fire extinguisher ready and familiarize yourself with how to use it. If the fire is small, it is possible to use a fire blanket to cut off its oxygen supply. If it is difficult to isolate or if it is a chemical fire, then it is better to use a CO<sub>2</sub> fire extinguisher.</p>
 +
</li>
 +
<li><p>Be aware of emergency exits.</p>
 +
</li>
 +
<li><p>Keep doors closed: This allows for proper ventilation, can allow for the maintenance of negative pressure in labs that require it, and might be helpful in case of fire.</p>
 +
</li>
 +
<li><p>Save emergency numbers on your phone: Fire brigade (118) and Security center of the lab and surrounding industrial area (+41 44 342 11 88).</p>
 +
</li>
 +
<li><p>When working with open fire keep hair short or use a hair tie.</p>
 +
</li>
 +
</b></td>
 +
<td>
 +
<img src="https://static.igem.org/mediawiki/2015/d/df/RiskB1.png" width="70%">
 +
</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td><b>I spilled and entire bottle of ethidium bromide on my lab coat!</b></td>
 +
<td>
 +
<img src="https://static.igem.org/mediawiki/2015/7/78/RiskD4.png" width="70%">
 +
</td>
 +
<td>
 +
<ul>
 +
<li><p>Wear lab coats and safety goggles at all times</p>
 +
</li>
 +
<li><p>Be concentrated and handle chemicals with extra care.</p>
 +
</li>
 +
<li><p>In case it still happens: Get out of the lab and take an emergency shower immediately.</p>
 +
</li>
 +
<li><p>Also take off potentially contaminated clothes.</p>
 +
</li>
 +
</b></td>
 +
<td>
 +
<img src="https://static.igem.org/mediawiki/2015/5/55/RiskC1.png" width="70%">
 +
</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td><b>I forgot to put my goggles on and now I have sulphuric acid in my eye!</b></td>
 +
<td>
 +
<img src="https://static.igem.org/mediawiki/2015/d/da/RiskC5.png" width="70%">
 +
</td>
 +
<td>
 +
<ul>
 +
<li><p>Wear lab coats and safety goggles at all times</p>
 +
</li>
 +
<li><p>Be concentrated and handle chemicals with extra care.</p>
 +
</li>
 +
<li><p>In case it still happens: There are eye washers in every lab. The eye(s) should be washed for about ten minutes.</p>
 +
</li>
 +
<li><p>Visit a doctor afterwards to check the state of the eye(s) and to see if it suffered any damage.</p>
 +
</li>
 +
</b></td>
 +
<td>
 +
<img src="https://static.igem.org/mediawiki/2015/d/df/RiskB1.png" width="70%">
 +
</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td><b>Dangerous equipment</b> </td>
 +
<td>
 +
<img src="https://static.igem.org/mediawiki/2015/7/71/RiskE4.png" width="70%">
 +
</td>
 +
<td>
 +
<ul>
 +
<li><p><i>Centrifuge</i>: must be always properly equilibrated. An unequilibrated centrifuge can be lethal if it dismantles at high speed.</p>
 +
</li>
 +
<li><p><i>Balance</i>: must be kept clean at all times! No one wants to find a suspicious unknown powder spread all over the place.</p>
 +
</li>
 +
</b></td>
 +
<td>
 +
<img src="https://static.igem.org/mediawiki/2015/d/d5/RiskD1.png" width="70%">
 +
</td>
 +
</tr>
 +
</table>
 +
 
 +
 
 +
 
 +
<h2>Handling Genetically Modified Organisms</h2>
 +
 
 +
 
 +
<p>Our MicroBeacon <i>E. coli</i> cells do not express any toxic substances and the fact that they prefer temperatures around 37 °C makes it unlikely that they survive for a long time when accidentally released. However, as they are genetically modified they might still harbor a potential and unforseen risk to the environment. </p>
 +
 
 +
 
 +
<table>
 +
<tr>
 +
<td><b>Possible Scenario</b></td>
 +
<td><b>Old Risk</b></td>
 +
<td><b>Countermeasures</b></td>
 +
<td><b>New Risk</b></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td><b><i>E.coli</i> infection</b></td>
 +
<td>
 +
<img src="https://static.igem.org/mediawiki/2015/7/75/RiskD3.png" width="70%">
 +
</td>
 +
<td>
 +
<ul>
 +
<li><p>Autoclave waste</p>
 +
</li>
 +
<li><p>Change gloves and wash hands frequently</p>
 +
</li>
 +
<li><p>Mimimize virulence: We decided to introduce as few antibiotic resistance genes as possible into our <i>E. coli</i> cells.</p>
 +
</li>
 +
</b></td>
 +
<td>
 +
<img src="https://static.igem.org/mediawiki/2015/d/df/RiskB1.png" width="70%">
 +
</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td><b><i>E.coli</i> released into environment</b></td>
 +
<td>
 +
<img src="https://static.igem.org/mediawiki/2015/b/b4/RiskB5.png" width="70%">
 +
</td>
 +
<td>
 +
<ul>
 +
<li><p>Autoclave waste.</p>
 +
</li>
 +
<li><p>Change gloves and wash hands frequently.</p>
 +
</li>
 +
<li><p>Clean benches and floor, disinfect shoes after work.</p>
 +
</li>
 +
</b></td>
 +
<td>
 +
<img src="https://static.igem.org/mediawiki/2015/d/df/RiskB1.png" width="70%">
 +
</td>
 +
</tr>
 +
</table>
 +
 
 +
 
 +
 
 +
 
 +
<h2>Using our MicroBeacon CTC Detection System for Medical Diagnosis</h2>
 +
 
 +
<p>It is never too early to think ahead what might happen if our MicroBeacon project is further developed and commercially used as a cancer diagnosis kit.  
 +
Since one component of the kit are live bacteria, special care has to be taken in a clinical environment.</p>
 +
 
 +
<table>
 +
<tr>
 +
<td><b>Possible Scenario</b></td>
 +
<td><b>Old Risk</b></td>
 +
<td><b>Countermeasures</b></td>
 +
<td><b>New Risk</b></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td><b><i>E.coli</i> infection</b></td>
 +
<td>
 +
<img src="https://static.igem.org/mediawiki/2015/7/71/RiskE4.png" width="70%">
 +
</td>
 +
<td>
 +
<ul>
 +
<li><p>Keep MicroBeacons away from patients: Collect blood sample and do the test experiment in a seperate room.</p>
 +
</li>
 +
<li><p>After use autoclave waste and destroy remaining blood sample or store in a safe container.</p>
 +
</li>
 +
<li><p>The test should be handled by trained medical personnel only.</p>
 +
</li>
 +
<li><p>Have suitable antibiotics ready.</p>
 +
</li>
 +
</b></td>
 +
<td>
 +
<img src="https://static.igem.org/mediawiki/2015/5/55/RiskC1.png" width="70%">
 +
</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td><b>False positive test result</b></td>
 +
<td>
 +
<img src="https://static.igem.org/mediawiki/2015/7/78/RiskD4.png" width="70%">
 +
</td>
 +
<td>
 +
<ul>
 +
<p>Consider the ethical implications of diagnosing cancer metastasis in a healthy individual.
 +
<li><p>Discretion is advised until the finding is confirmed with an alternative test.</p>
 +
</li>
 +
<li><p>Carefully calibrate the test equipment and use fresh control samples.</p>
 +
</li>
 +
<li><p>Repeat the test multiple times.</p>
 +
</li>
 +
<li><p>Inform the patient about possible uncertainties of the test outcome.</p>
 +
</li>
 +
</b></td>
 +
<td>
 +
<img src="https://static.igem.org/mediawiki/2015/5/55/RiskC1.png" width="70%">
 +
</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td><b>Production error</b></td>
 +
<td>
 +
<img src="https://static.igem.org/mediawiki/2015/b/bc/RiskD5.png" width="70%">
 +
</td>
 +
<td>
 +
<ul>
 +
<p>On the side of the manufacturer: consider the finanial risk and possible damage to the company's reputation</p>
 +
<li><p>Perform routine quality controls over the entire manufacturing process and at customer level.</p>
 +
</li>
 +
<li><p>Keep track of the sold devices with serial numbers and customer details. Recall faulty devices immediately if necessary.</p>
 +
</li>
 +
<li><p>Inform customers about unlikely but possible equipment failure. Replace faulty devices free of charge but reject legal liability with a disclaimer.</p>
 +
</li>
 +
<li><p>Offer sufficient online and hands-on support for customers.</p>
 +
</li>
 +
</b></td>
 +
<td>
 +
<img src="https://static.igem.org/mediawiki/2015/d/df/RiskB1.png" width="70%">
 +
</td>
 +
</tr>
 +
</table>
 +
 
 +
</div>
 
</div>
 
</div>
 
</html>
 
</html>
 +
{{:Template:ETH_Zurich/footer}}

Latest revision as of 01:24, 19 September 2015

"What I cannot create I do not understand."
- Richard Feynmann

General Safety Considerations

Method of Safety Evaluation

To manage the risks associated with the development of our MicroBeacon CTC detection system we used the following scheme to evaluate possible adverse scenarios and the effectiveness of countermeasures:

Preventive measures can either serve to decrease the likelihood (x-axis) or the severity (y-axis) of a potential scenario or both. The goal is to move the risks as far as possible into the green corner.

Working at the ETH Zurich Labs

Responsible conduct during everyday lab hours is parmount for the prevention of accidents. Every new person in the ETH D-BSSE lab must undergo safety training instructed by a Technical Manager. No exception was made for our iGEM team. Though much of it is common sense and our work was restricted to Biosafety Level 1, there is still a lot that can go wrong. Here are a few examples:

Possible Scenario Old Risk Countermeasures New Risk
Accidental poisoning

  • Wear lab coats and safety goggles at all times

  • Wear gloves, be concentrated and handle chemicals with extra care.

  • No smoking, eating or drinking is in the lab.

  • Chemicals have to be properly labelled.

  • Just in case save emergency numbers on your phone: Ambulance (144) and Center for Toxicology (145)
My E. coli are on fire!

    Since we use Bunsen burners to create a semi-sterile working environment it is quite likely that something catches fire.

  • Keep flamable items (paper, cloth, flamable chemicals) away from the flame.

  • Have a fire extinguisher ready and familiarize yourself with how to use it. If the fire is small, it is possible to use a fire blanket to cut off its oxygen supply. If it is difficult to isolate or if it is a chemical fire, then it is better to use a CO2 fire extinguisher.

  • Be aware of emergency exits.

  • Keep doors closed: This allows for proper ventilation, can allow for the maintenance of negative pressure in labs that require it, and might be helpful in case of fire.

  • Save emergency numbers on your phone: Fire brigade (118) and Security center of the lab and surrounding industrial area (+41 44 342 11 88).

  • When working with open fire keep hair short or use a hair tie.
I spilled and entire bottle of ethidium bromide on my lab coat!

  • Wear lab coats and safety goggles at all times

  • Be concentrated and handle chemicals with extra care.

  • In case it still happens: Get out of the lab and take an emergency shower immediately.

  • Also take off potentially contaminated clothes.
I forgot to put my goggles on and now I have sulphuric acid in my eye!

  • Wear lab coats and safety goggles at all times

  • Be concentrated and handle chemicals with extra care.

  • In case it still happens: There are eye washers in every lab. The eye(s) should be washed for about ten minutes.

  • Visit a doctor afterwards to check the state of the eye(s) and to see if it suffered any damage.
Dangerous equipment

  • Centrifuge: must be always properly equilibrated. An unequilibrated centrifuge can be lethal if it dismantles at high speed.

  • Balance: must be kept clean at all times! No one wants to find a suspicious unknown powder spread all over the place.

Handling Genetically Modified Organisms

Our MicroBeacon E. coli cells do not express any toxic substances and the fact that they prefer temperatures around 37 °C makes it unlikely that they survive for a long time when accidentally released. However, as they are genetically modified they might still harbor a potential and unforseen risk to the environment.

Possible Scenario Old Risk Countermeasures New Risk
E.coli infection

  • Autoclave waste

  • Change gloves and wash hands frequently

  • Mimimize virulence: We decided to introduce as few antibiotic resistance genes as possible into our E. coli cells.
E.coli released into environment

  • Autoclave waste.

  • Change gloves and wash hands frequently.

  • Clean benches and floor, disinfect shoes after work.

Using our MicroBeacon CTC Detection System for Medical Diagnosis

It is never too early to think ahead what might happen if our MicroBeacon project is further developed and commercially used as a cancer diagnosis kit. Since one component of the kit are live bacteria, special care has to be taken in a clinical environment.

Possible Scenario Old Risk Countermeasures New Risk
E.coli infection

  • Keep MicroBeacons away from patients: Collect blood sample and do the test experiment in a seperate room.

  • After use autoclave waste and destroy remaining blood sample or store in a safe container.

  • The test should be handled by trained medical personnel only.

  • Have suitable antibiotics ready.
False positive test result

    Consider the ethical implications of diagnosing cancer metastasis in a healthy individual.

  • Discretion is advised until the finding is confirmed with an alternative test.

  • Carefully calibrate the test equipment and use fresh control samples.

  • Repeat the test multiple times.

  • Inform the patient about possible uncertainties of the test outcome.
Production error

    On the side of the manufacturer: consider the finanial risk and possible damage to the company's reputation

  • Perform routine quality controls over the entire manufacturing process and at customer level.

  • Keep track of the sold devices with serial numbers and customer details. Recall faulty devices immediately if necessary.

  • Inform customers about unlikely but possible equipment failure. Replace faulty devices free of charge but reject legal liability with a disclaimer.

  • Offer sufficient online and hands-on support for customers.

We would like to thank our sponsors