Difference between revisions of "Team:HUST-China/Background"

 
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  <title>Team:HUST-China:Background</title>
 
  <title>Team:HUST-China:Background</title>
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          <ul class="nav">
 
          <ul class="nav">
 
              <li class="first-menu"><a href="https://2015.igem.org/Team:HUST-China">HOME</a>
 
              <li class="first-menu"><a href="https://2015.igem.org/Team:HUST-China">HOME</a>
 
                </li>      
 
                </li>      
                <li class="dropdown first-menu" id="accountmenu"><a href="https://2015.igem.org/Team:HUST-China/Description">PROJECT<b class="caret"></b></a>
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                <li class="dropdown other-menu" id="accountmenu"><a href="https://2015.igem.org/Team:HUST-China/Project">PROJECT<b class="caret"></b></a>
 
                <ul class="dropdown-menu">
 
                <ul class="dropdown-menu">
                <li><a href="https://2015.igem.org/Team:HUST-China/Background">Background</a></li>
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                                <li><a href="https://2015.igem.org/Team:HUST-China/Background">Background</a></li>
 
                <li class="divider"></li>
 
                <li class="divider"></li>
 
                <li><a href="https://2015.igem.org/Team:HUST-China/Description">Description</a></li>
 
                <li><a href="https://2015.igem.org/Team:HUST-China/Description">Description</a></li>
 
                <li class="divider"></li>
 
                <li class="divider"></li>
                        <li><a href="https://2015.igem.org/Team:HUST-China/Experiments">Experiments&protocol</a></li>
 
                        <li class="divider"></li>
 
                        <li><a href="https://2015.igem.org/Team:HUST-China/Results">Results</a></li>
 
                        <li class="divider"></li>
 
 
                        <li><a href="https://2015.igem.org/Team:HUST-China/Design">Design</a></li>           
 
                        <li><a href="https://2015.igem.org/Team:HUST-China/Design">Design</a></li>           
 
                    </ul>
 
                    </ul>
 
                         </li>
 
                         </li>
  
                         <li class="dropdown first-menu" id="accountmenu"><a href="https://2015.igem.org/Team:HUST-China/Parts">PARTS<b class="caret"></b></a>
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                         <li class="dropdown other-menu" id="accountmenu"><a href="https://2015.igem.org/Team:HUST-China/Wetlab">WETLAB<b class="caret"></b></a>
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                <ul class="dropdown-menu">
 +
<li><a href="https://2015.igem.org/Team:HUST-China/Results">Results</a></li>
 +
                        <li class="divider"></li>                      
 +
                        <li><a href="https://2015.igem.org/Team:HUST-China/Experiments">Experiments&protocol</a></li>
 
                        <li class="divider"></li>
 
                        <li class="divider"></li>
 +
                        <li><a href="https://2015.igem.org/Team:HUST-China/InterLab Study">InterLab Study</a></li>
 +
                        <li class="divider"></li>
 +
                        <li><a href="https://2015.igem.org/Team:HUST-China/Notebook">Notebook</a></li>     
 +
                    </ul>
 +
                        </li>
 +
 +
                        <li class="dropdown other-menu" id="accountmenu"><a href="https://2015.igem.org/Team:HUST-China/Parts">PARTS<b class="caret"></b></a>
 +
                <ul class="dropdown-menu">                        
 
                        <li><a href="https://2015.igem.org/Team:HUST-China/Basic_part">Basic Parts</a></li>
 
                        <li><a href="https://2015.igem.org/Team:HUST-China/Basic_part">Basic Parts</a></li>
 
                        <li class="divider"></li>
 
                        <li class="divider"></li>
                        <li><a href="https://2015.igem.org/Team:HUST-China/Composite_part">Composite Parts</a></li>
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                        <li><a href="https://2015.igem.org/Team:HUST-China/Basic_part#2">Composite Parts</a></li>                           <li class="divider"></li>
                        <li class="divider"></li>
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                        <li><a href="https://2015.igem.org/Team:HUST-China/Part_Collection">Part Collection</a></li>  
                        <li><a href="https://2015.igem.org/Team:HUST-China/Part _Collection">Part Collection</a></li>      
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                    </ul>
 
                    </ul>
 
                         </li>
 
                         </li>
 
                 
 
                 
                <li class="dropdown first-menu" id="accountmenu"><a href="https://2015.igem.org/Team:HUST-China/Modeling">MODELING</a> </li>
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                <li class="dropdown other-menu" id="accountmenu"><a href="https://2015.igem.org/Team:HUST-China/Modeling">MODELING<b class="caret"></b></a>  
 +
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 +
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 +
                                        <li class="divider"></li>
 +
                          <li><a href="https://2015.igem.org/Team:HUST-China/Modeling on Cellular Level">Modeling on Cellular Level</a></li>
 +
                          <li class="divider"></li>
 +
                        <li><a href="https://2015.igem.org/Team:HUST-China/Modeling on Ecosystem Level">Modeling on Ecosystem Level</a></li>
 +
                    </ul>
 +
                </li>
  
                <li class="dropdown first-menu" id="accountmenu"><a href="https://2015.igem.org/Team:HUST-China//Team">TEAM</a>         </li>                
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+
                <li class="dropdown other-menu" id="accountmenu"><a href="https://2015.igem.org/Team:HUST-China/Practices">HUMAN PRACTICES</a>
                <li class="dropdown first-menu" id="accountmenu">
+
                                       
                    <a class="dropdown-toggle" data-toggle="dropdown" href="https://2015.igem.org/Team:HUST-China/Practices">HUMAN PRACTICES<b class="caret"></b></a>
+
                          <ul class="dropdown-menu">
+
                          <li><a href="https://2015.igem.org/Team:HUST-China/Safety">Safety</a></li>                                    
+
                    </ul>                
+
 
                </li>
 
                </li>
  
              <li class="dropdown other-menu" id="accountmenu">
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              <li class="dropdown other-menu" id="accountmenu"><a href="https://2015.igem.org/Team:HUST-China/Safety">OTHERS<b class="caret"></b></a>
                    <a class="dropdown-toggle" data-toggle="dropdown" href="https://2015.igem.org/Team:HUST-China#">OUTREACH<b class="caret"></b></a>
+
 
                    <ul class="dropdown-menu">
 
                    <ul class="dropdown-menu">
                          <li><a href="https://2015.igem.org/Team:HUST-China/Attributions">Attributions</a></li>
+
                        <li><a href="https://2015.igem.org/Team:HUST-China/Safety">Safety</a></li>
 
                        <li class="divider"></li>
 
                        <li class="divider"></li>
                        <li><a href="https://2015.igem.org/Team:HUST-China/Collaboration">Collaboration</a></li>
+
                        <li><a href="https://2015.igem.org/Team:HUST-China/Collaborations">Collaborations</a></li>
                        <li class="divider"></li>                    
+
                        <li><a href="https://2015.igem.org/Team:HUST-China/Measurement">Measurement</a></li>
+
 
                        <li class="divider"></li>
 
                        <li class="divider"></li>
                        <li><a href="https://2015.igem.org/Team:HUST-China/Notebook">Notebook</a></li>
+
                        <li><a href="https://2015.igem.org/Team:HUST-China/Attributions">Attributions</a></li>
                        <li class="divider"></li>
+
                        <li class="divider"></li>                  
                        <li><a href="https://2015.igem.org/Team:HUST-China/Measurement">InterLab Study</a></li>
+
                        <li><a href="https://2015.igem.org/Team:HUST-China/Team">Team</a></li>
 +
                        <li class="divider"></li>                   
 +
                        <li><a href="https://2015.igem.org/Team:HUST-China/Achievements">Achievements</a></li>
 
                    </ul>
 
                    </ul>
 
              </li>
 
              </li>
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<!--标题栏-->
 
<!--标题栏-->
 
  <div id="pic" >
 
  <div id="pic" >
  <div class="wenzi" align="center"><b><br><br><br><br><br><br>
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  <img class="title" src="https://static.igem.org/mediawiki/2015/8/87/HUST_PROJECT_2.PNG"/>
Background</b><br><br><br>
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  <br>
<div style="font-size:30px" align="center">A live eukaryotic cell based auto-cementation kit</div>  
+
  </div>
+
<br><br><br>
+
 
  <div class="pic_a" >
 
  <div class="pic_a" >
<h4 style="color:white">Scroll down to read more</h4>
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<h3 align="center" style="color:white"><b>click it~</b></h3>
 
      <img style="cursor:pointer;" id="to_des" src="https://static.igem.org/mediawiki/2015/8/80/White.png"/>
 
      <img style="cursor:pointer;" id="to_des" src="https://static.igem.org/mediawiki/2015/8/80/White.png"/>
 
  </div>
 
  </div>
 
  </div>
 
  </div>
  
    <!--锚点-->
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<div id="maodian">
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<img src="https://static.igem.org/mediawiki/2015/d/da/Maodian.png" >
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<ul class="ul" >
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<li class="li"><a href="#description" class="btn btn-default btn-lg">Description</a></li>
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<li class="li"><a href="#darkness" class="btn btn-default btn-lg">Darkness induction system</a></li>
+
<li class="li"><a href="#supporting" class="btn btn-default btn-lg">Supporting system</a></li>
+
<li class="li"><a href="#flocculating" class="btn btn-default btn-lg">Flocculating system</a></li>
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<li class="li"><a href="#host" class="btn btn-default btn-lg">Host strain</a></li>
+
<li class="li"><a href="#improvement" class="btn btn-default btn-lg">Improvement</a></li>
+
<li class="li"><a href="#reference" class="btn btn-default btn-lg">References</a></li>
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</ul>
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</div>
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  <!--描述-->
 
  <!--描述-->
 
 
 
 
 
     <div align="center" class="description"><a name="description"></a><br><br>
 
     <div align="center" class="description"><a name="description"></a><br><br>
         <p>With the rapid growth of economy in China, many environmental problems come along. In northern China, the Inner Mongolia grassland is facing increasingly severe desertification[1]; in Hangzhou Bay and Pearl River Delta where cross-sea bridges were built, the loose soil at the base constantly troubles the minds of the constructors and security departments[2]; along the coast of the South China Sea[3], marine ecology damages such as the recession of coral reefs caused by overfishing also becomes a world-concern problem. However, a vital technology may be the solution to all of these problems above—the reinforcement technology. The reinforcement of land and sea soil can efficiently hold the water on the grassland, strengthen the basal soil of cross-sea bridges and restore the marine ecology environment. Therefore, we set our project theme on combining the reinforcement technology with synthesis biology.
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<h2 style="color:black" align="center"><b>Background</b></h2><br>
 +
         <p>With the rapid growth of economy in China, many environmental problems come along. In northern China, the Inner Mongolia grassland is facing increasingly severe desertification<sup>[1-2]</sup> ; in Hangzhou Bay and Pearl River Delta where cross-sea bridges were built, the loose soil at the base constantly troubles the constructors and security department<sup>[3-4]</sup>; along the coast of the South China Sea <sup>[5]</sup>, marine ecology damages such as the recession of coral reefs caused by overfishing and trawl abuse also becomes a world-concern problem. However, a vital technology may be the solution to all of these problems above—the reinforcement technology. The reinforcement of soil can efficiently hold the water on the degenerative grassland, strengthen the basal soil of cross-sea bridges and restore the marine ecology environment of sea bed. Therefore, we set our project theme on combining the reinforcement technology with synthesis biology.
 
     </p>
 
     </p>
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     <img class="picture" src="https://static.igem.org/mediawiki/2015/7/74/HUST_background1.png">
 
     <img class="picture" src="https://static.igem.org/mediawiki/2015/7/74/HUST_background1.png">
<h4 align="center">Picture 1:Hangzhou Bay Bridge piers far lightmaps</h4><br>
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<div class="pizhu">Figure 1:Hangzhou Bay Bridge piers far lightmaps</div>
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</div>
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<div class="box">
 
<img class="picture" src="https://static.igem.org/mediawiki/2015/0/0d/HUST_background2.png">
 
<img class="picture" src="https://static.igem.org/mediawiki/2015/0/0d/HUST_background2.png">
<h4 align="center">Picture 2:Hangzhou Bay Bridge Piers close-shots</h4><br>
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<div class="pizhu">Figure 2:Hangzhou Bay Bridge Piers close-shots</div>
 +
</div>
 +
<div class="box">
 
<img class="picture" src="https://static.igem.org/mediawiki/2015/a/a4/HUST_background3.png">
 
<img class="picture" src="https://static.igem.org/mediawiki/2015/a/a4/HUST_background3.png">
<h4 align="center">Picture 3:The city facing the danger of desertification</h4><br>
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<div class="pizhu">Figure 3:The city facing the danger of desertification</div>
 +
</div>
 +
<div class="box">
 
<img class="picture" src="https://static.igem.org/mediawiki/2015/e/e6/HUST_background4.png">
 
<img class="picture" src="https://static.igem.org/mediawiki/2015/e/e6/HUST_background4.png">
<h4 align="center">Picture 4:The devastated coral reefs</h4><br>
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<div class="pizhu">Figure 4:The devastated coral reefs</div>
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</div>
 +
<br><br>
  
<p>The existing reinforcement technologies usually tend to chemical or physical approaches and employ synthetic polymers as the curing agent. These polymers are toxic and nondegradable in the environment, thus will bring contamination and damages for a long time.</p>
+
<p>The existing reinforcement technologies usually tend to chemical or physical approaches and employ synthetic polymers as the curing agent. These polymers are toxic and undegradable in natural environment, thus it will bring contamination and damages for a long time.</p>
<p>Comparing with the traditional methods, the microbial reinforcement technology [6], an emerging environment-friendly technology, shows its superiority. The principle of microbial reinforcement is adding urea and calcium chloride solution to the soil with bacillus strain in it. Under the induction of calcium ion, the strain will produce calcium carbonate which will deposit and cement the soil to perform the reinforcing function.</p>
+
<p>
<p>Our project is inspired by the microbial reinforcement technology and we further developed it in the light of synthesis biology. We developed a reinforcement kit in order to apply our method in many similar problems. The kit includes the control system, the supporting system and the flocculation system. </p>
+
Comparing with the traditional methods, the microbial reinforcement technology<sup>[6]</sup> , an emerging environment-friendly technology, shows its superiority. The principle of microbial reinforcement is adding urea and calcium chloride solution to the soil with bacillus strain in it. Under the induction of calcium ion, the strain will produce calcium carbonate which will deposit and cement the soil to perform the reinforcing function.</p>
<p>Our engineering yeast is named Euk.cement, i.e. the eukaryote used to cement.</p>  
+
<p>
<p>Nowadays, the coral reef recession has rung alarm to the whole world. There are over 10% of coral reefs that are damaged and another 30% to 80% under threat of human activity. According to the statistics released by The Nature Conservancy, the recession speed of coral reefs is increasing and 70% of coral reefs will disappear in 50 years. Under this stressful circumstance, we hope that our engineering yeast can be used in the construction of artificial reefs and make an effort in restoring marine environment and saving the endangered coral reefs.</p>
+
Our project is inspired by the microbial reinforcement technology and we further developed it in the light of synthesis biology. We developed a reinforcement kit in order to apply our method in many similar problems. The kit includes a control system, a supporting system and a flocculation system. We engineered eukaryote and included the up mentioned 3 systems into it. This kit was named Euk.cement, i.e. the eukaryote used for cementation.</p>
<p>Due to the seriousness of the reefs problem, we focus on the issue of artificial reefs to restore marine ecosystems. With the reinforcement kit, we use specific parts to collect and reinforce the floating sand in the sea and form artificial reefs and use Yarrowia lipolytica as the chassis to suit the demand of marine environment.</p>
+
<p>Nowadays, the coral reef recession has rung alarm to the whole world. There are over 10% of coral reefs that are damaged and another 30% to 80% under threat of human activity. According to the statistics released by The Nature Conservancy, the recession speed of coral reefs is increasing and 70% of coral reefs will disappear in 50 years. Under this stressful circumstance, we hope that our engineered yeast can be used in the construction of artificial reefs and make an effort in restoring marine environment and saving the endangered coral reefs. Briefly speaking, with our reinforcement kit, we can use Euk.cement to collect and reinforce the hydraulic filled scattered sands onto regional limited seabed and form artificial reefs. Here we use Yarrowia lipolytica as the chassis to suit the demand of work in marine environment.
<p><a href="https://2015.igem.org/Team:HUST-China/Description">To know what Euk.Cement is exactly, please turn to <i><b>description.</b></i></a></p>
+
</p>
    </p>
+
<p>
 +
<a href="https://2015.igem.org/Team:HUST-China/Description">To know what Euk.Cement is exactly, please turn to <i><b>description.</b></i></a>
 +
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         <h2 style="color:black" align="left"><b>Reference</b></h2>
 
         <h2 style="color:black" align="left"><b>Reference</b></h2>
 
         <p>
 
         <p>
         [1] Ash R F, Edmonds R L. China's land resources, environment and agricultural production[J]. The China Quarterly, 1998, 156: 836-879.</p>
+
         [1] Ash R F, Edmonds R L. China's land resources, environment and agricultural production[J]. The China Quarterly, 1998, 156: 836-879.<br>
<p>[2]Diamond Jared “Collapse.” Pp.364-5.Penguins Books,2005.</p>
+
[2]Diamond Jared “Collapse.” Pp.364-5.Penguins Books,2005.<br>
<p>[3]Andrea Ding-Kemp.Enr.construction.com. Retrieved 2010-07-05.</p>
+
[3]Andrea Ding-Kemp.Enr.construction.com. Retrieved 2010-07-05.<br>
<p>[4] Murray N J, Clemens R S, Phinn S R, et al. Tracking the rapid loss of tidal wetlands in the Yellow Sea[J]. Frontiers in Ecology and the Environment, 2014, 12(5): 267-272.</p>
+
[4] Murray N J, Clemens R S, Phinn S R, et al. Tracking the rapid loss of tidal wetlands in the Yellow Sea[J]. Frontiers in Ecology and the Environment, 2014, 12(5): 267-272.<br>
<p>[5]MacKinnon, J.; Verkuil, Y.I.; Murray, N.J. (2012), Asian Coastal Wetlands.</p>
+
[5]MacKinnon, J.; Verkuil, Y.I.; Murray, N.J. (2012), Asian Coastal Wetlands<br>
<p>[6]Jroundi F, Gómez-Suaga P, Jimenez-Lopez C, et al. Stone-isolated carbonatogenic bacteria as inoculants in bioconsolidation treatments for historical limestone[J]. Science of the total environment, 2012, 425: 89-98.</p>
+
[6]Jroundi F, Gómez-Suaga P, Jimenez-Lopez C, et al. Stone-isolated.<br> carbonatogenic bacteria as inoculants in bioconsolidation treatments for historical limestone[J]. Science of the total environment, 2012, 425: 89-98.<br><br><br>
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Latest revision as of 01:46, 19 September 2015

Team:HUST-China:Background


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Background


With the rapid growth of economy in China, many environmental problems come along. In northern China, the Inner Mongolia grassland is facing increasingly severe desertification[1-2] ; in Hangzhou Bay and Pearl River Delta where cross-sea bridges were built, the loose soil at the base constantly troubles the constructors and security department[3-4]; along the coast of the South China Sea [5], marine ecology damages such as the recession of coral reefs caused by overfishing and trawl abuse also becomes a world-concern problem. However, a vital technology may be the solution to all of these problems above—the reinforcement technology. The reinforcement of soil can efficiently hold the water on the degenerative grassland, strengthen the basal soil of cross-sea bridges and restore the marine ecology environment of sea bed. Therefore, we set our project theme on combining the reinforcement technology with synthesis biology.

Figure 1:Hangzhou Bay Bridge piers far lightmaps
Figure 2:Hangzhou Bay Bridge Piers close-shots
Figure 3:The city facing the danger of desertification
Figure 4:The devastated coral reefs


The existing reinforcement technologies usually tend to chemical or physical approaches and employ synthetic polymers as the curing agent. These polymers are toxic and undegradable in natural environment, thus it will bring contamination and damages for a long time.

Comparing with the traditional methods, the microbial reinforcement technology[6] , an emerging environment-friendly technology, shows its superiority. The principle of microbial reinforcement is adding urea and calcium chloride solution to the soil with bacillus strain in it. Under the induction of calcium ion, the strain will produce calcium carbonate which will deposit and cement the soil to perform the reinforcing function.

Our project is inspired by the microbial reinforcement technology and we further developed it in the light of synthesis biology. We developed a reinforcement kit in order to apply our method in many similar problems. The kit includes a control system, a supporting system and a flocculation system. We engineered eukaryote and included the up mentioned 3 systems into it. This kit was named Euk.cement, i.e. the eukaryote used for cementation.

Nowadays, the coral reef recession has rung alarm to the whole world. There are over 10% of coral reefs that are damaged and another 30% to 80% under threat of human activity. According to the statistics released by The Nature Conservancy, the recession speed of coral reefs is increasing and 70% of coral reefs will disappear in 50 years. Under this stressful circumstance, we hope that our engineered yeast can be used in the construction of artificial reefs and make an effort in restoring marine environment and saving the endangered coral reefs. Briefly speaking, with our reinforcement kit, we can use Euk.cement to collect and reinforce the hydraulic filled scattered sands onto regional limited seabed and form artificial reefs. Here we use Yarrowia lipolytica as the chassis to suit the demand of work in marine environment.

To know what Euk.Cement is exactly, please turn to description.



Reference

[1] Ash R F, Edmonds R L. China's land resources, environment and agricultural production[J]. The China Quarterly, 1998, 156: 836-879.
[2]Diamond Jared “Collapse.” Pp.364-5.Penguins Books,2005.
[3]Andrea Ding-Kemp.Enr.construction.com. Retrieved 2010-07-05.
[4] Murray N J, Clemens R S, Phinn S R, et al. Tracking the rapid loss of tidal wetlands in the Yellow Sea[J]. Frontiers in Ecology and the Environment, 2014, 12(5): 267-272.
[5]MacKinnon, J.; Verkuil, Y.I.; Murray, N.J. (2012), Asian Coastal Wetlands
[6]Jroundi F, Gómez-Suaga P, Jimenez-Lopez C, et al. Stone-isolated.
carbonatogenic bacteria as inoculants in bioconsolidation treatments for historical limestone[J]. Science of the total environment, 2012, 425: 89-98.