Difference between revisions of "Team:OUC-China"

 
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    <script type="text/javascript" src="https://2015.igem.org/Team:OUC-China/js/index?action=raw&ctype=text/javascript"></script>
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                 <div class="banner-top">
 
                 <div class="banner-top">
 
                     <ul class="nav banner-nav">
 
                     <ul class="nav banner-nav">
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                         <li><a href="index.html">HOME</a></li>
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                         <li><a href="https://2015.igem.org/Team:OUC-China">HOME</a></li>
 
                         <li class="dropdown1"><a class="down-scroll" href="#">PROJECT</a>
 
                         <li class="dropdown1"><a class="down-scroll" href="#">PROJECT</a>
 
                             <ul class="dropdown2">
 
                             <ul class="dropdown2">
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                                 <li><a href="#">Overview</a></li>
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                                 <li><a href="https://2015.igem.org/Team:OUC-China/Project/Overview">Overview</a></li>
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                                 <li><a href="#">Magnetic Receiver</a></li>
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                                 <li><a href="https://2015.igem.org/Team:OUC-China/Project/Magnetic_Receiver">Magnetic Receiver</a></li>
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                                 <li><a href="#">Thermosensitive Regulator</a></li>
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                                 <li><a href="https://2015.igem.org/Team:OUC-China/Project/Thermosensitive_Regulator">Thermosensitive Regulator</a></li>
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                                 <li><a href="#">Captor</a></li>
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                                 <li><a href="https://2015.igem.org/Team:OUC-China/Project/Captor">Captor</a></li>
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                                 <li><a href="#">Results</a></li>
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                                 <li><a href="https://2015.igem.org/Team:OUC-China/Project/Results">Results</a></li>
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                                 <li><a href="#">Parts</a></li>
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                                 <li><a href="https://2015.igem.org/Team:OUC-China/Parts">Parts</a></li>
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                                 <li><a href="#">Achievements</a></li>
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                                 <li><a href="https://2015.igem.org/Team:OUC-China/Project/Achievements">Achievements</a></li>
 
                             </ul>
 
                             </ul>
 
                         </li>
 
                         </li>
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                         <li class="dropdown1"><a href="#">INTERLAB</a>
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                         <li class="dropdown1"><a href="https://2015.igem.org/Team:OUC-China/Interlab">INTERLAB</a>
 
                         </li>
 
                         </li>
 
                         <li class="dropdown1"><a class="down-scroll" href="#">MODELING</a>
 
                         <li class="dropdown1"><a class="down-scroll" href="#">MODELING</a>
 
                             <ul class="dropdown2">
 
                             <ul class="dropdown2">
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                                 <li><a href="#">Overview</a></li>
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                                 <li><a href="https://2015.igem.org/Team:OUC-China/Modeling">Overview</a></li>
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                                 <li><a href="#">Magnetic Receiver</a></li>
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                                 <li><a href="https://2015.igem.org/Team:OUC-China/Modeling/Magnetic_Receiver">Magnetic Receiver</a></li>
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                                 <li><a href="#">Heating</a></li>
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                                 <li><a href="https://2015.igem.org/Team:OUC-China/Modeling/Heating">Heating</a></li>
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                                 <li><a href="#">Thermosensitive Regulator</a></li>
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                                 <li><a href="https://2015.igem.org/Team:OUC-China/Modeling/Thermosensitive_Regulator">Thermosensitive Regulator</a></li>
 
                             </ul>
 
                             </ul>
 
                         </li>
 
                         </li>
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                         <li class="dropdown1"><a class="down-scroll" href="">HUMAN PRACTISE</a>
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                         <li class="dropdown1"><a class="down-scroll" href="#">HUMAN PRACTISE</a>
 
                             <ul class="dropdown2">
 
                             <ul class="dropdown2">
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                                 <li><a href="#">Overview</a></li>
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                                 <li><a href="https://2015.igem.org/Team:OUC-China/Practices">Overview</a></li>
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                                 <li><a href="#">Safety</a></li>
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                                 <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Safety">Safety</a></li>
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                                 <li><a href="#">Education</a></li>
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                                 <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Education">Education</a></li>
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                                 <li><a href="#">Transportation</a></li>
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                                 <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Transportation">Transportation</a></li>
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                                 <li><a href="#">Communication</a></li>
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                                 <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Communication">Communication</a></li>
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                                 <li><a href="#">Guide of Logo Design</a></li>
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                                 <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Guide_Of_Logo_Design">Guide of Logo Design</a></li>
 
                             </ul>
 
                             </ul>
 
                         </li>
 
                         </li>
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                         <li class="dropdown1"><a class="down-scroll" href="">NOTE BOOK</a>
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                         <li class="dropdown1"><a class="down-scroll" href="#">NOTE BOOK</a>
 
                             <ul class="dropdown2">
 
                             <ul class="dropdown2">
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                                 <li><a href="#">Lab Journal</a></li>
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                                 <li><a href="https://2015.igem.org/Team:OUC-China/Note_Book/Lab_Journal">Lab Journal</a></li>
−
                                 <li><a href="#">Protocol</a></li>
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                                 <li><a href="https://2015.igem.org/Team:OUC-China/Note_Book/Protocol">Protocol</a></li>
 
                             </ul>
 
                             </ul>
 
                         </li>
 
                         </li>
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                         <li class="dropdown1"><a class="down-scroll" href="">TEAM</a>
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                         <li class="dropdown1"><a class="down-scroll" href="#">TEAM</a>
 
                             <ul class="dropdown2">
 
                             <ul class="dropdown2">
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                                 <li><a href="#">Member</a></li>
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                                 <li><a href="https://2015.igem.org/Team:OUC-China/Team/Member">Member</a></li>
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                                 <li><a href="#">Attribution</a></li>
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                                 <li><a href="https://2015.igem.org/Team:OUC-China/Attributions">Attribution</a></li>
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                                 <li><a href="#">Collaboration</a></li>
+
                                 <li><a href="https://2015.igem.org/Team:OUC-China/Collaborations">Collaboration</a></li>
 
                             </ul>
 
                             </ul>
 
                         </li>
 
                         </li>
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     <div class="banner-container">
 
     <div class="banner-container">
 
         <div class="banner-content">
 
         <div class="banner-content">
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             <img src="https://static.igem.org/mediawiki/2015/4/4b/OUC-China-Team_Logo.png" alt="" class="img-responsive center-block">
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             <img src="https://static.igem.org/mediawiki/2015/4/4b/OUC-China-Team_Logo.png" width="400" alt="" class="img-responsive center-block">
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             <h2>Platform for Electromagnetic Control</h2>
+
             <h2>Magthermo coli</h2>
 
         </div>
 
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                     <img src="https://static.igem.org/mediawiki/2015/e/e1/OUC-China-home_receiver_regulator1.png" alt="" class="img-responsive">
 
                     <img src="https://static.igem.org/mediawiki/2015/e/e1/OUC-China-home_receiver_regulator1.png" alt="" class="img-responsive">
 
                     <h3 class="text-uppercase text-center">step 1</h3>
 
                     <h3 class="text-uppercase text-center">step 1</h3>
−
                     <p>Our Magthermocoli contains 2 core components: <B>magnetic receiver & thermosensitive regulator.</B></p>
+
                     <p>Our Magthermo coli contains 2 core components: <B>Magnetic Receiver & Thermosensitive Regulator.</B></p>
 
                 </div>
 
                 </div>
 
                 <div class="col-md-4">
 
                 <div class="col-md-4">
 
                     <img src="https://static.igem.org/mediawiki/2015/e/e7/OUC-China-home_receiver_regulator2.png" alt="" class="img-responsive">
 
                     <img src="https://static.igem.org/mediawiki/2015/e/e7/OUC-China-home_receiver_regulator2.png" alt="" class="img-responsive">
 
                     <h3 class="text-uppercase text-center">step 2</h3>
 
                     <h3 class="text-uppercase text-center">step 2</h3>
−
                     <p>
+
                     <p>Inducing with electromagnetic field, magnetic receiver will heat, raising the ambient temperature.</p>
−
                        Induced by electromagnetic field，magnetic receiver would be heated, thus raise the ambient temperature.
+
−
                    </p>
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                 </div>
 
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                     <h3 class="text-uppercase text-center">step 3</h3>
 
                     <h3 class="text-uppercase text-center">step 3</h3>
 
                     <p>
 
                     <p>
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                         In response to the change of temperature, thermosensitive regulator would initiate GFP (or RFP) expression.
+
                         In response to the change of temperature, thermosensitive regulator will initiate downstream gene (GFP for example) expression.
 
                     </p>
 
                     </p>
 
                 </div>
 
                 </div>
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                     <div class="caption">
 
                     <div class="caption">
 
                         <p>
 
                         <p>
−
                             For magnetic receiver,we chose <B>Ferritin</B>, the iron-storage protein in organisms, which could synthesizeferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core would be heated, raising the ambient temperature.
+
                             For magnetic receiver,we chose <B>Ferritin</B>, the iron-storage protein in many organisms, which could synthesize ferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core will heat, raising the ambient temperature.
 
                         </p>
 
                         </p>
 
                         <p>
 
                         <p>
−
                             In this session, we explored measurement techniques for the valuation of magnetism、biomineralization and Protein concentration per cell.
+
                             In this section, we explored measurement techniques for the valuation of magnetism, biomineralization and protein concentration per cell.
 
                         </p>
 
                         </p>
−
                         <p><a href="#" class="btn btn-primary" role="button">More details</a></p>
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                         <p><a href="https://2015.igem.org/Team:OUC-China/Project/Magnetic_Receiver" class="btn btn-primary" role="button">More details</a></p>
 
                     </div>
 
                     </div>
 
                 </div>
 
                 </div>
 
                 <div class="col-md-5">
 
                 <div class="col-md-5">
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                     <img src="https://static.igem.org/mediawiki/2015/d/d3/OUC-China-home_frritin.png" alt="" class="img-responsive">
+
                     <img src="https://static.igem.org/mediawiki/2015/1/17/OUC-China-home-frritin.png" alt="" class="img-responsive">
 
                 </div>
 
                 </div>
 
             </div>
 
             </div>
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             <div class="row">
 
                 <div class="col-md-6">
 
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                     <img src="https://static.igem.org/mediawiki/2015/c/cf/OUC-China-home_RNA.png" alt="" class="img-responsive">
+
                     <img src="https://static.igem.org/mediawiki/2015/b/b8/OUC-China-home-RNA.png" alt="" class="img-responsive">
 
                 </div>
 
                 </div>
 
                 <div class="col-md-6">
 
                 <div class="col-md-6">
 
                     <div class="caption">
 
                     <div class="caption">
 
                         <p>
 
                         <p>
−
                             <B>RNA thermometer</B> is a structured RNA which could expose RBS only at appropriate temperature.  In this session, we explored the measurement method to identify the efficiency of thermosensitive regulator under heat stress.
+
                             <B>RNA thermometer</B> is a structured RNA which can expose SD sequences only at appropriate temperature.  In this section, we explored the measurement method to identify the efficiency of thermosensitive regulator under heat stress.
 
                         </p>
 
                         </p>
−
                         <p><a href="#" class="btn btn-primary" role="button">More details</a></p>
+
                         <p><a href="https://2015.igem.org/Team:OUC-China/Project/Thermosensitive_Regulator" class="btn btn-primary" role="button">More details</a></p>
 
                     </div>
 
                     </div>
 
                 </div>
 
                 </div>
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                     <div class="caption">
 
                     <div class="caption">
 
                         <p>
 
                         <p>
−
                             <B>Thermosensitive T7 RNA polymerase</B> is a normal T7 RNA polymerase at whose selected locus was inserted by a temperature-sensitive intein, which could self-splice at specific temperature.
+
                             <B>Thermosensitive T7 RNA polymerase</B> is a T7 RNA polymerase interrupted by a temperature-sensitive intein, which can self-splice at specific temperature and initiate the downstream signal.
 
                         </p>
 
                         </p>
−
                         <p><a href="#" class="btn btn-primary" role="button">More details</a></p>
+
                         <p><a href="https://2015.igem.org/Team:OUC-China/Project/Thermosensitive_Regulator" class="btn btn-primary" role="button">More details</a></p>
 
                     </div>
 
                     </div>
 
                 </div>
 
                 </div>
 
                 <div class="col-md-6">
 
                 <div class="col-md-6">
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                     <img src="https://static.igem.org/mediawiki/2015/9/9e/OUC-China-home_T7.png" alt="" class="img-responsive">
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                     <img src="https://static.igem.org/mediawiki/2015/4/4d/OUC-China-home-T7.png" alt="" class="img-responsive">
 
                 </div>
 
                 </div>
 
             </div>
 
             </div>
 
         </div>
 
         </div>
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    </div>
 +
    <div class="returnTop">
 +
        <img src="https://static.igem.org/mediawiki/2015/a/a4/ReturnTop.png" alt="">
 
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         <div class="container">
 
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                 Follow us on Facebook@ <a href="#">iGEM OUC</a>
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                 Follow us on Facebook@ <a href="https://www.facebook.com/oucigem">iGEM OUC</a>
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                 mail to: <a href="mailto:#">oucigem@163.com</a>
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                 <br>Mail to: <a href="mailto:#">oucigem@163.com</a>
 
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Latest revision as of 01:50, 19 September 2015

<!DOCTYPE html> Team:OUC-China

how does it work?

This summer we designed Magthermo coli——a platform for remote regulation of gene expression by electromagnetic signal.

step 1

Our Magthermo coli contains 2 core components: Magnetic Receiver & Thermosensitive Regulator.

step 2

Inducing with electromagnetic field, magnetic receiver will heat, raising the ambient temperature.

step 3

In response to the change of temperature, thermosensitive regulator will initiate downstream gene (GFP for example) expression.

Magnetic Receiver

For magnetic receiver,we chose Ferritin, the iron-storage protein in many organisms, which could synthesize ferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core will heat, raising the ambient temperature.

In this section, we explored measurement techniques for the valuation of magnetism, biomineralization and protein concentration per cell.

More details

Thermosensitive Regulator

For thermosensitive regulator，we chose RNA thermometer and designed a thermosensitive T7 RNA polymerase.

RNA thermometer is a structured RNA which can expose SD sequences only at appropriate temperature. In this section, we explored the measurement method to identify the efficiency of thermosensitive regulator under heat stress.

More details

Thermosensitive T7 RNA polymerase is a T7 RNA polymerase interrupted by a temperature-sensitive intein, which can self-splice at specific temperature and initiate the downstream signal.

More details