Difference between revisions of "Team:OUC-China"
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#contentSub, #contentcontainer, #footer-box, #sitesub, #catlinks, #search-controls, #p-logo, .h3, .printfooter, .firstHeading, .visualClear {display: none;} /*-- hides default wiki settings --*/ | #contentSub, #contentcontainer, #footer-box, #sitesub, #catlinks, #search-controls, #p-logo, .h3, .printfooter, .firstHeading, .visualClear {display: none;} /*-- hides default wiki settings --*/ | ||
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<div class="banner-container"> | <div class="banner-container"> | ||
<div class="banner-content"> | <div class="banner-content"> | ||
| − | <img src="https://static.igem.org/mediawiki/2015/4/4b/OUC-China-Team_Logo.png" width=" | + | <img src="https://static.igem.org/mediawiki/2015/4/4b/OUC-China-Team_Logo.png" width="400" alt="" class="img-responsive center-block"> |
| − | <h2> | + | <h2>Magthermo coli</h2> |
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<img src="https://static.igem.org/mediawiki/2015/e/e1/OUC-China-home_receiver_regulator1.png" alt="" class="img-responsive"> | <img src="https://static.igem.org/mediawiki/2015/e/e1/OUC-China-home_receiver_regulator1.png" alt="" class="img-responsive"> | ||
<h3 class="text-uppercase text-center">step 1</h3> | <h3 class="text-uppercase text-center">step 1</h3> | ||
| − | <p>Our Magthermo coli contains 2 core components: <B> | + | <p>Our Magthermo coli contains 2 core components: <B>Magnetic Receiver & Thermosensitive Regulator.</B></p> |
</div> | </div> | ||
<div class="col-md-4"> | <div class="col-md-4"> | ||
<img src="https://static.igem.org/mediawiki/2015/e/e7/OUC-China-home_receiver_regulator2.png" alt="" class="img-responsive"> | <img src="https://static.igem.org/mediawiki/2015/e/e7/OUC-China-home_receiver_regulator2.png" alt="" class="img-responsive"> | ||
<h3 class="text-uppercase text-center">step 2</h3> | <h3 class="text-uppercase text-center">step 2</h3> | ||
| − | <p>Inducing with electromagnetic | + | <p>Inducing with electromagnetic field, magnetic receiver will heat, raising the ambient temperature.</p> |
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| − | For magnetic receiver,we chose <B>Ferritin</B>, the iron-storage protein in many organisms, which | + | For magnetic receiver,we chose <B>Ferritin</B>, the iron-storage protein in many organisms, which could synthesize ferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core will heat, raising the ambient temperature. |
</p> | </p> | ||
<p> | <p> | ||
In this section, we explored measurement techniques for the valuation of magnetism, biomineralization and protein concentration per cell. | In this section, we explored measurement techniques for the valuation of magnetism, biomineralization and protein concentration per cell. | ||
</p> | </p> | ||
| − | <p><a href=" | + | <p><a href="https://2015.igem.org/Team:OUC-China/Project/Magnetic_Receiver" class="btn btn-primary" role="button">More details</a></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="col-md-5"> | <div class="col-md-5"> | ||
| − | <img src="https://static.igem.org/mediawiki/2015/ | + | <img src="https://static.igem.org/mediawiki/2015/1/17/OUC-China-home-frritin.png" alt="" class="img-responsive"> |
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| − | <img src="https://static.igem.org/mediawiki/2015/ | + | <img src="https://static.igem.org/mediawiki/2015/b/b8/OUC-China-home-RNA.png" alt="" class="img-responsive"> |
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<B>RNA thermometer</B> is a structured RNA which can expose SD sequences only at appropriate temperature. In this section, we explored the measurement method to identify the efficiency of thermosensitive regulator under heat stress. | <B>RNA thermometer</B> is a structured RNA which can expose SD sequences only at appropriate temperature. In this section, we explored the measurement method to identify the efficiency of thermosensitive regulator under heat stress. | ||
</p> | </p> | ||
| − | <p><a href=" | + | <p><a href="https://2015.igem.org/Team:OUC-China/Project/Thermosensitive_Regulator" class="btn btn-primary" role="button">More details</a></p> |
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<div class="caption"> | <div class="caption"> | ||
<p> | <p> | ||
| − | <B>Thermosensitive T7 RNA polymerase</B> is a T7 RNA polymerase interrupted by a temperature-sensitive intein, which | + | <B>Thermosensitive T7 RNA polymerase</B> is a T7 RNA polymerase interrupted by a temperature-sensitive intein, which can self-splice at specific temperature and initiate the downstream signal. |
</p> | </p> | ||
| − | <p><a href=" | + | <p><a href="https://2015.igem.org/Team:OUC-China/Project/Thermosensitive_Regulator" class="btn btn-primary" role="button">More details</a></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="col-md-6"> | <div class="col-md-6"> | ||
| − | <img src="https://static.igem.org/mediawiki/2015/ | + | <img src="https://static.igem.org/mediawiki/2015/4/4d/OUC-China-home-T7.png" alt="" class="img-responsive"> |
</div> | </div> | ||
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| + | <div class="returnTop"> | ||
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</div> | </div> | ||
<footer> | <footer> | ||
<div class="container"> | <div class="container"> | ||
<address class="center-block"> | <address class="center-block"> | ||
| − | Follow us on Facebook@ <a href=" | + | Follow us on Facebook@ <a href="https://www.facebook.com/oucigem">iGEM OUC</a> |
<br>Mail to: <a href="mailto:#">oucigem@163.com</a> | <br>Mail to: <a href="mailto:#">oucigem@163.com</a> | ||
</address> | </address> | ||
| − | <img src="https://static.igem.org/mediawiki/2015/ | + | <center><ul class="footer-img"> |
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Latest revision as of 01:50, 19 September 2015
<!DOCTYPE html>
Magthermo coli
how does it work?
This summer we designed Magthermo coli——a platform for remote regulation of gene expression by electromagnetic signal.
step 1
Our Magthermo coli contains 2 core components: Magnetic Receiver & Thermosensitive Regulator.
step 2
Inducing with electromagnetic field, magnetic receiver will heat, raising the ambient temperature.
step 3
In response to the change of temperature, thermosensitive regulator will initiate downstream gene (GFP for example) expression.
Magnetic Receiver
For magnetic receiver,we chose Ferritin, the iron-storage protein in many organisms, which could synthesize ferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core will heat, raising the ambient temperature.
In this section, we explored measurement techniques for the valuation of magnetism, biomineralization and protein concentration per cell.
Thermosensitive Regulator
For thermosensitive regulator，we chose RNA thermometer and designed a thermosensitive T7 RNA polymerase.
RNA thermometer is a structured RNA which can expose SD sequences only at appropriate temperature. In this section, we explored the measurement method to identify the efficiency of thermosensitive regulator under heat stress.
Thermosensitive T7 RNA polymerase is a T7 RNA polymerase interrupted by a temperature-sensitive intein, which can self-splice at specific temperature and initiate the downstream signal.
