Difference between revisions of "Team:OUC-China"
Freezingcool (Talk | contribs) |
|||
| (13 intermediate revisions by 4 users not shown) | |||
| Line 22: | Line 22: | ||
.banner-top{ | .banner-top{ | ||
margin-left: 12%; | margin-left: 12%; | ||
| + | } | ||
| + | @media (min-width: 992px){ | ||
| + | .work-content .col-md-4{ | ||
| + | width:25%; | ||
| + | margin-right: 8%; | ||
| + | } | ||
} | } | ||
#contentSub, #contentcontainer, #footer-box, #sitesub, #catlinks, #search-controls, #p-logo, .h3, .printfooter, .firstHeading, .visualClear {display: none;} /*-- hides default wiki settings --*/ | #contentSub, #contentcontainer, #footer-box, #sitesub, #catlinks, #search-controls, #p-logo, .h3, .printfooter, .firstHeading, .visualClear {display: none;} /*-- hides default wiki settings --*/ | ||
| Line 121: | Line 127: | ||
<div class="banner-content"> | <div class="banner-content"> | ||
<img src="https://static.igem.org/mediawiki/2015/4/4b/OUC-China-Team_Logo.png" width="400" alt="" class="img-responsive center-block"> | <img src="https://static.igem.org/mediawiki/2015/4/4b/OUC-China-Team_Logo.png" width="400" alt="" class="img-responsive center-block"> | ||
| − | <h2> | + | <h2>Magthermo coli</h2> |
</div> | </div> | ||
</div> | </div> | ||
| Line 138: | Line 144: | ||
<img src="https://static.igem.org/mediawiki/2015/e/e1/OUC-China-home_receiver_regulator1.png" alt="" class="img-responsive"> | <img src="https://static.igem.org/mediawiki/2015/e/e1/OUC-China-home_receiver_regulator1.png" alt="" class="img-responsive"> | ||
<h3 class="text-uppercase text-center">step 1</h3> | <h3 class="text-uppercase text-center">step 1</h3> | ||
| − | <p>Our Magthermo coli contains 2 core components: <B> | + | <p>Our Magthermo coli contains 2 core components: <B>Magnetic Receiver & Thermosensitive Regulator.</B></p> |
</div> | </div> | ||
<div class="col-md-4"> | <div class="col-md-4"> | ||
<img src="https://static.igem.org/mediawiki/2015/e/e7/OUC-China-home_receiver_regulator2.png" alt="" class="img-responsive"> | <img src="https://static.igem.org/mediawiki/2015/e/e7/OUC-China-home_receiver_regulator2.png" alt="" class="img-responsive"> | ||
<h3 class="text-uppercase text-center">step 2</h3> | <h3 class="text-uppercase text-center">step 2</h3> | ||
| − | <p>Inducing with electromagnetic | + | <p>Inducing with electromagnetic field, magnetic receiver will heat, raising the ambient temperature.</p> |
</div> | </div> | ||
<div class="col-md-4"> | <div class="col-md-4"> | ||
| Line 162: | Line 168: | ||
<div class="caption"> | <div class="caption"> | ||
<p> | <p> | ||
| − | For magnetic receiver,we chose <B>Ferritin</B>, the iron-storage protein in many organisms, which | + | For magnetic receiver,we chose <B>Ferritin</B>, the iron-storage protein in many organisms, which could synthesize ferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core will heat, raising the ambient temperature. |
</p> | </p> | ||
<p> | <p> | ||
| Line 171: | Line 177: | ||
</div> | </div> | ||
<div class="col-md-5"> | <div class="col-md-5"> | ||
| − | <img src="https://static.igem.org/mediawiki/2015/ | + | <img src="https://static.igem.org/mediawiki/2015/1/17/OUC-China-home-frritin.png" alt="" class="img-responsive"> |
</div> | </div> | ||
</div> | </div> | ||
| Line 182: | Line 188: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-md-6"> | <div class="col-md-6"> | ||
| − | <img src="https://static.igem.org/mediawiki/2015/ | + | <img src="https://static.igem.org/mediawiki/2015/b/b8/OUC-China-home-RNA.png" alt="" class="img-responsive"> |
</div> | </div> | ||
<div class="col-md-6"> | <div class="col-md-6"> | ||
| Line 197: | Line 203: | ||
<div class="caption"> | <div class="caption"> | ||
<p> | <p> | ||
| − | <B>Thermosensitive T7 RNA polymerase</B> is a T7 RNA polymerase interrupted by a temperature-sensitive intein, which | + | <B>Thermosensitive T7 RNA polymerase</B> is a T7 RNA polymerase interrupted by a temperature-sensitive intein, which can self-splice at specific temperature and initiate the downstream signal. |
</p> | </p> | ||
<p><a href="https://2015.igem.org/Team:OUC-China/Project/Thermosensitive_Regulator" class="btn btn-primary" role="button">More details</a></p> | <p><a href="https://2015.igem.org/Team:OUC-China/Project/Thermosensitive_Regulator" class="btn btn-primary" role="button">More details</a></p> | ||
| Line 203: | Line 209: | ||
</div> | </div> | ||
<div class="col-md-6"> | <div class="col-md-6"> | ||
| − | <img src="https://static.igem.org/mediawiki/2015/ | + | <img src="https://static.igem.org/mediawiki/2015/4/4d/OUC-China-home-T7.png" alt="" class="img-responsive"> |
</div> | </div> | ||
</div> | </div> | ||
| Line 214: | Line 220: | ||
<div class="container"> | <div class="container"> | ||
<address class="center-block"> | <address class="center-block"> | ||
| − | Follow us on Facebook@ <a href=" | + | Follow us on Facebook@ <a href="https://www.facebook.com/oucigem">iGEM OUC</a> |
<br>Mail to: <a href="mailto:#">oucigem@163.com</a> | <br>Mail to: <a href="mailto:#">oucigem@163.com</a> | ||
</address> | </address> | ||
| − | <img src="https://static.igem.org/mediawiki/2015/ | + | <center><ul class="footer-img"> |
| + | <li> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/2/27/OUC-China_footer1.png" alt="" class="img-responsive"> | ||
| + | </li> | ||
| + | <li> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/7/7c/OUC-China_footer2.png" alt="" class="img-responsive"> | ||
| + | </li> | ||
| + | <li> | ||
| + | <a href="http://www.genewiz.com/"><img src="https://static.igem.org/mediawiki/2015/0/08/OUC-China_footer3.png" alt="" class="img-responsive"></a> | ||
| + | </li> | ||
| + | <li> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/2/26/OUC-China_footer4.png" alt="" class="img-responsive"> | ||
| + | </li> | ||
| + | <li> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/3/3e/OUC-China_footer5.png" alt="" class="img-responsive"> | ||
| + | </li> | ||
| + | <div class="clr"></div> | ||
| + | </ul></center> | ||
</div> | </div> | ||
</footer> | </footer> | ||
Latest revision as of 01:50, 19 September 2015
<!DOCTYPE html>
Magthermo coli
how does it work?
This summer we designed Magthermo coli——a platform for remote regulation of gene expression by electromagnetic signal.
step 1
Our Magthermo coli contains 2 core components: Magnetic Receiver & Thermosensitive Regulator.
step 2
Inducing with electromagnetic field, magnetic receiver will heat, raising the ambient temperature.
step 3
In response to the change of temperature, thermosensitive regulator will initiate downstream gene (GFP for example) expression.
Magnetic Receiver
For magnetic receiver,we chose Ferritin, the iron-storage protein in many organisms, which could synthesize ferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core will heat, raising the ambient temperature.
In this section, we explored measurement techniques for the valuation of magnetism, biomineralization and protein concentration per cell.
Thermosensitive Regulator
For thermosensitive regulator，we chose RNA thermometer and designed a thermosensitive T7 RNA polymerase.
RNA thermometer is a structured RNA which can expose SD sequences only at appropriate temperature. In this section, we explored the measurement method to identify the efficiency of thermosensitive regulator under heat stress.
Thermosensitive T7 RNA polymerase is a T7 RNA polymerase interrupted by a temperature-sensitive intein, which can self-splice at specific temperature and initiate the downstream signal.
