Difference between revisions of "Team:OUC-China"

 
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<div class="head">
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            <div class="container">
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<div id="navLogoLeft">
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                <span class="menu"> Menu</span>
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        <a href="https://igem.org/Team.cgi?year=2015&amp;team_name=China_Tongji">
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                <div class="banner-top">
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                    <ul class="nav banner-nav">
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         </a>
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                        <li><a href="https://2015.igem.org/Team:OUC-China">HOME</a></li>
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                        <li class="dropdown1"><a class="down-scroll" href="#">PROJECT</a>
 +
                            <ul class="dropdown2">
 +
                                <li><a href="https://2015.igem.org/Team:OUC-China/Project/Overview">Overview</a></li>
 +
                                <li><a href="https://2015.igem.org/Team:OUC-China/Project/Magnetic_Receiver">Magnetic Receiver</a></li>
 +
                                <li><a href="https://2015.igem.org/Team:OUC-China/Project/Thermosensitive_Regulator">Thermosensitive Regulator</a></li>
 +
                                <li><a href="https://2015.igem.org/Team:OUC-China/Project/Captor">Captor</a></li>
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Project/Results">Results</a></li>
 +
                                <li><a href="https://2015.igem.org/Team:OUC-China/Parts">Parts</a></li>
 +
                                <li><a href="https://2015.igem.org/Team:OUC-China/Project/Achievements">Achievements</a></li>
 +
                            </ul>
 +
                        </li>
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                        <li class="dropdown1"><a href="https://2015.igem.org/Team:OUC-China/Interlab">INTERLAB</a>
 +
                        </li>
 +
                        <li class="dropdown1"><a class="down-scroll" href="#">MODELING</a>
 +
                            <ul class="dropdown2">
 +
                                <li><a href="https://2015.igem.org/Team:OUC-China/Modeling">Overview</a></li>
 +
                                <li><a href="https://2015.igem.org/Team:OUC-China/Modeling/Magnetic_Receiver">Magnetic Receiver</a></li>
 +
                                <li><a href="https://2015.igem.org/Team:OUC-China/Modeling/Heating">Heating</a></li>
 +
                                <li><a href="https://2015.igem.org/Team:OUC-China/Modeling/Thermosensitive_Regulator">Thermosensitive Regulator</a></li>
 +
                            </ul>
 +
                        </li>
 +
                        <li class="dropdown1"><a class="down-scroll"  href="#">HUMAN PRACTISE</a>
 +
                            <ul class="dropdown2">
 +
                                <li><a href="https://2015.igem.org/Team:OUC-China/Practices">Overview</a></li>
 +
                                <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Safety">Safety</a></li>
 +
                                <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Education">Education</a></li>
 +
                                <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Transportation">Transportation</a></li>
 +
                                <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Communication">Communication</a></li>
 +
                                <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Guide_Of_Logo_Design">Guide of Logo Design</a></li>
 +
                            </ul>
 +
                        </li>
 +
                        <li class="dropdown1"><a class="down-scroll" href="#">NOTE BOOK</a>
 +
                            <ul class="dropdown2">
 +
                                <li><a href="https://2015.igem.org/Team:OUC-China/Note_Book/Lab_Journal">Lab Journal</a></li>
 +
                                <li><a href="https://2015.igem.org/Team:OUC-China/Note_Book/Protocol">Protocol</a></li>
 +
                            </ul>
 +
                        </li>
 +
                        <li class="dropdown1"><a class="down-scroll" href="#">TEAM</a>
 +
                            <ul class="dropdown2">
 +
                                <li><a href="https://2015.igem.org/Team:OUC-China/Team/Member">Member</a></li>
 +
                                <li><a href="https://2015.igem.org/Team:OUC-China/Attributions">Attribution</a></li>
 +
                                <li><a href="https://2015.igem.org/Team:OUC-China/Collaborations">Collaboration</a></li>
 +
                            </ul>
 +
                        </li>
 +
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            <h2>Magthermo coli</h2>
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                     how does it <span>work?</span>
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                     This summer we designed Magthermo coli——a platform for remote regulation of gene expression by electromagnetic signal.
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                    <img src="https://static.igem.org/mediawiki/2015/e/e1/OUC-China-home_receiver_regulator1.png" alt="" class="img-responsive">
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                 <li class="navTwo" id="navRecord"><a href="https://2015.igem.org/Team:China_Tongji/Notebook">Record</a></li>
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                    <h3 class="text-uppercase text-center">step 1</h3>
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                 <li class="navTwo" id="navTimeline"><a href="https://2015.igem.org/Team:China_Tongji/Notebook">Timeline</a></li>
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                    <p>Our Magthermo coli contains 2 core components: <B>Magnetic Receiver & Thermosensitive Regulator.</B></p>
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                 </div>
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                <div class="col-md-4">
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                    <img src="https://static.igem.org/mediawiki/2015/e/e7/OUC-China-home_receiver_regulator2.png" alt="" class="img-responsive">
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                    <h3 class="text-uppercase text-center">step 2</h3>
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                    <p>Inducing with electromagnetic field, magnetic receiver will heat, raising the ambient temperature.</p>
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                    <img src="https://static.igem.org/mediawiki/2015/6/6d/OUC-China_home_receiver_regulator3.png" alt="" class="img-responsive">
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                    <h3 class="text-uppercase text-center">step 3</h3>
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                    <p>
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                        In response to the change of temperature, thermosensitive regulator will initiate downstream gene (GFP for example) expression.
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            <h3 class="text-center index-headline">Magnetic <span>Receiver</span></h3>
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                            For magnetic receiver,we chose <B>Ferritin</B>, the iron-storage protein in many organisms, which could synthesize ferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core will heat, raising the ambient temperature.
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                        </p>
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                        <p>
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                            In this section, we explored measurement techniques for the valuation of magnetism, biomineralization and protein concentration per cell.
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                        </p>
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                        <p><a href="https://2015.igem.org/Team:OUC-China/Project/Magnetic_Receiver" class="btn btn-primary" role="button">More details</a></p>
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            <h3 class="text-center index-headline index-headline2">Thermosensitive <span>Regulator</span></h3>
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            <p class="text-center">For thermosensitive regulator，we chose <B>RNA thermometer</B> and designed a <B>thermosensitive T7 RNA polymerase.</B> </p>
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                        <p>
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                            <B>RNA thermometer</B> is a structured RNA which can expose SD sequences only at appropriate temperature.  In this section, we explored the measurement method to identify the efficiency of thermosensitive regulator under heat stress.
 +
                        </p>
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                        <p><a href="https://2015.igem.org/Team:OUC-China/Project/Thermosensitive_Regulator" class="btn btn-primary" role="button">More details</a></p>
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                            <B>Thermosensitive T7 RNA polymerase</B> is a T7 RNA polymerase interrupted by a temperature-sensitive intein, which can self-splice at specific temperature and initiate the downstream signal.
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                        <p><a href="https://2015.igem.org/Team:OUC-China/Project/Thermosensitive_Regulator" class="btn btn-primary" role="button">More details</a></p>
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                Follow us on Facebook@ <a href="https://www.facebook.com/oucigem">iGEM OUC</a>
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                <br>Mail to: <a href="mailto:#">oucigem@163.com</a>
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    <p class="contentP">In our project, we will use the technology of optogenetic and use light of different wave produced by the special light source assembled by ourselves to control the moving of C.elegans, construct a movement controlling system and bulid an amusement park of C.elegans.</p>
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<p class="contentP">We will try to design our special parts and express channalrhodopsin in specific C.elegans' neurons. To accomplish our goals to express channalrhodopsin in single neuron, we make the use of cre- loxp system and the overlapping of promoters. We not only use the traditional channalrhodopsin,chR2,but also try to express the novel channelrhodopsin, Blink, and other fancy channelrhodopsins which have never been tested in C.elegans.</p>
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how does it work?

This summer we designed Magthermo coli——a platform for remote regulation of gene expression by electromagnetic signal.

step 1

Our Magthermo coli contains 2 core components: Magnetic Receiver & Thermosensitive Regulator.

step 2

Inducing with electromagnetic field, magnetic receiver will heat, raising the ambient temperature.

step 3

In response to the change of temperature, thermosensitive regulator will initiate downstream gene (GFP for example) expression.

Magnetic Receiver

For magnetic receiver,we chose Ferritin, the iron-storage protein in many organisms, which could synthesize ferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core will heat, raising the ambient temperature.

In this section, we explored measurement techniques for the valuation of magnetism, biomineralization and protein concentration per cell.

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Thermosensitive Regulator

For thermosensitive regulator，we chose RNA thermometer and designed a thermosensitive T7 RNA polymerase.

RNA thermometer is a structured RNA which can expose SD sequences only at appropriate temperature. In this section, we explored the measurement method to identify the efficiency of thermosensitive regulator under heat stress.

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Thermosensitive T7 RNA polymerase is a T7 RNA polymerase interrupted by a temperature-sensitive intein, which can self-splice at specific temperature and initiate the downstream signal.

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