Difference between revisions of "Team:OUC-China"

 
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                        <li><a href="https://2015.igem.org/Team:OUC-China">HOME</a></li>
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                        <li class="dropdown1"><a class="down-scroll" href="#">PROJECT</a>
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Project/Overview">Overview</a></li>
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Project/Magnetic_Receiver">Magnetic Receiver</a></li>
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Project/Thermosensitive_Regulator">Thermosensitive Regulator</a></li>
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Project/Captor">Captor</a></li>
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                        <li class="dropdown1"><a href="https://2015.igem.org/Team:OUC-China/Interlab">INTERLAB</a>
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Modeling">Overview</a></li>
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Modeling/Magnetic_Receiver">Magnetic Receiver</a></li>
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Modeling/Heating">Heating</a></li>
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Modeling/Thermosensitive_Regulator">Thermosensitive Regulator</a></li>
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Safety">Safety</a></li>
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Education">Education</a></li>
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Communication">Communication</a></li>
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Guide_Of_Logo_Design">Guide of Logo Design</a></li>
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                            </ul>
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Note_Book/Lab_Journal">Lab Journal</a></li>
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Note_Book/Protocol">Protocol</a></li>
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                            </ul>
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                        </li>
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                        <li class="dropdown1"><a class="down-scroll" href="#">TEAM</a>
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Team/Member">Member</a></li>
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Attributions">Attribution</a></li>
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Collaborations">Collaboration</a></li>
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            <h2>Magthermo coli</h2>
 
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  <div class="headertiltle"><a href="https://2015.igem.org/Team:BostonU">Boston University iGEM 2015  </a></div>
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                <h2 class="text-uppercase">
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                    how does it <span>work?</span>
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                </h2>
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                <p>
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                    This summer we designed Magthermo coli——a platform for remote regulation of gene expression by electromagnetic signal.
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                </p>
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            </div>
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            <div class="row work-content">
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                <div class="col-md-4">
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                    <img src="https://static.igem.org/mediawiki/2015/e/e1/OUC-China-home_receiver_regulator1.png" alt="" class="img-responsive">
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                    <h3 class="text-uppercase text-center">step 1</h3>
 +
                    <p>Our Magthermo coli contains 2 core components: <B>Magnetic Receiver & Thermosensitive Regulator.</B></p>
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                </div>
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                <div class="col-md-4">
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                    <img src="https://static.igem.org/mediawiki/2015/e/e7/OUC-China-home_receiver_regulator2.png" alt="" class="img-responsive">
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                    <h3 class="text-uppercase text-center">step 2</h3>
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                    <p>Inducing with electromagnetic field, magnetic receiver will heat, raising the ambient temperature.</p>
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                </div>
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                <div class="col-md-4">
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                    <img src="https://static.igem.org/mediawiki/2015/6/6d/OUC-China_home_receiver_regulator3.png" alt="" class="img-responsive">
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                    <h3 class="text-uppercase text-center">step 3</h3>
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                    <p>
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                        In response to the change of temperature, thermosensitive regulator will initiate downstream gene (GFP for example) expression.
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                    </p>
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    <div class="container-fluid index-details1 index-details">
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        <div class="container">
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            <h3 class="text-center index-headline">Magnetic <span>Receiver</span></h3>
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            <div class="row">
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                <div class="col-md-7">
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                    <div class="caption">
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                        <p>
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                            For magnetic receiver,we chose <B>Ferritin</B>, the iron-storage protein in many organisms, which could synthesize ferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core will heat, raising the ambient temperature.
 +
                        </p>
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                        <p>
 +
                            In this section, we explored measurement techniques for the valuation of magnetism, biomineralization and protein concentration per cell.
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                        </p>
 +
                        <p><a href="https://2015.igem.org/Team:OUC-China/Project/Magnetic_Receiver" class="btn btn-primary" role="button">More details</a></p>
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                    </div>
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                </div>
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                <div class="col-md-5">
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                    <img src="https://static.igem.org/mediawiki/2015/1/17/OUC-China-home-frritin.png" alt="" class="img-responsive">
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                </div>
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        </div>
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    </div>
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    <div class="container-fluid index-details2 index-details">
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        <div class="container">
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            <h3 class="text-center index-headline index-headline2">Thermosensitive <span>Regulator</span></h3>
 +
            <p class="text-center">For thermosensitive regulator，we chose <B>RNA thermometer</B> and designed a <B>thermosensitive T7 RNA polymerase.</B>  </p>
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            <div class="row">
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                    <img src="https://static.igem.org/mediawiki/2015/b/b8/OUC-China-home-RNA.png" alt="" class="img-responsive">
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                </div>
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                        <p>
 +
                            <B>RNA thermometer</B> is a structured RNA which can expose SD sequences only at appropriate temperature.  In this section, we explored the measurement method to identify the efficiency of thermosensitive regulator under heat stress.
 +
                        </p>
 +
                        <p><a href="https://2015.igem.org/Team:OUC-China/Project/Thermosensitive_Regulator" class="btn btn-primary" role="button">More details</a></p>
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                    </div>
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                </div>
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            </div>
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            <div class="row">
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                <div class="col-md-6">
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                    <div class="caption">
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                        <p>
 +
                            <B>Thermosensitive T7 RNA polymerase</B> is a T7 RNA polymerase interrupted by a temperature-sensitive intein, which can self-splice at specific temperature and initiate the downstream signal.
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                        </p>
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                        <p><a href="https://2015.igem.org/Team:OUC-China/Project/Thermosensitive_Regulator" class="btn btn-primary" role="button">More details</a></p>
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                    </div>
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                    <img src="https://static.igem.org/mediawiki/2015/4/4d/OUC-China-home-T7.png" alt="" class="img-responsive">
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    The goal of our team this summer is to create an efficient and widely applicable workflow for splitting proteins. By splitting proteins and fusing each half to a drug-inducible domain, scientists can gain temporal control over protein expression. Using our workflow, the proteins will be translated into two inert halves that are each fused to domains that bind in the presence of an inducer drug. By introducing the drug into the system, the two inert protein halves will come together and for a fully functioning protein. In this way, scientists can further increase their control over protein function. The two types of proteins we will be testing our workflow on are the large serine integrase family and saCAS9. These proteins harness powerful mechanisms that have significant applications in the future of synthetic biology. By using our workflow, we hope to increase scientists understanding of these proteins and also provide a mechanism for increasing temporal control.
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Latest revision as of 01:50, 19 September 2015

<!DOCTYPE html> Team:OUC-China

how does it work?

This summer we designed Magthermo coli——a platform for remote regulation of gene expression by electromagnetic signal.

step 1

Our Magthermo coli contains 2 core components: Magnetic Receiver & Thermosensitive Regulator.

step 2

Inducing with electromagnetic field, magnetic receiver will heat, raising the ambient temperature.

step 3

In response to the change of temperature, thermosensitive regulator will initiate downstream gene (GFP for example) expression.

Magnetic Receiver

For magnetic receiver,we chose Ferritin, the iron-storage protein in many organisms, which could synthesize ferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core will heat, raising the ambient temperature.

In this section, we explored measurement techniques for the valuation of magnetism, biomineralization and protein concentration per cell.

More details

Thermosensitive Regulator

For thermosensitive regulator，we chose RNA thermometer and designed a thermosensitive T7 RNA polymerase.

RNA thermometer is a structured RNA which can expose SD sequences only at appropriate temperature. In this section, we explored the measurement method to identify the efficiency of thermosensitive regulator under heat stress.

More details

Thermosensitive T7 RNA polymerase is a T7 RNA polymerase interrupted by a temperature-sensitive intein, which can self-splice at specific temperature and initiate the downstream signal.

More details