Difference between revisions of "Team:OUC-China"

 
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<meta name="keywords" content="integrated,evolution,machine,sysu-china,b2h,mutation,m13,RNAT">
 
<meta name="author" content="Moriwifi Guo Wenyan,MaryZhang Liu Chancheng" />
 
<title>2014 SYSU-China integrated evolution machine</title>
 
 
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                <span class="menu"> Menu</span>
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                <div class="banner-top">
<div class="intro">
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                    <ul class="nav banner-nav">
<h1>In The Past</h1>
+
                        <li><a href="https://2015.igem.org/Team:OUC-China">HOME</a></li>
<p>
+
                        <li class="dropdown1"><a class="down-scroll" href="#">PROJECT</a>
Directed Evolution experiments were so laborious.
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                            <ul class="dropdown2">
</p>
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Project/Overview">Overview</a></li>
 
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Project/Magnetic_Receiver">Magnetic Receiver</a></li>
<!--this is IGEM LOGO-->
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Project/Thermosensitive_Regulator">Thermosensitive Regulator</a></li>
            <div id="IGEMlogo" >
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Project/Captor">Captor</a></li>
            <a href="https://2014.igem.org/Main_Page">
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Project/Results">Results</a></li>
<img src="https://static.igem.org/mediawiki/2014/d/d7/Sysuchina_igemdeLogo.png" alt="IGEM LOGO" >
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Parts">Parts</a></li>
</a>
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Project/Achievements">Achievements</a></li>
</div>
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                            </ul>
 
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                        </li>
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                        <li class="dropdown1"><a href="https://2015.igem.org/Team:OUC-China/Interlab">INTERLAB</a>
 
+
                        </li>
<div class="worker">
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                        <li class="dropdown1"><a class="down-scroll" href="#">MODELING</a>
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Modeling">Overview</a></li>
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Modeling/Magnetic_Receiver">Magnetic Receiver</a></li>
<img src="https://static.igem.org/mediawiki/2014/8/83/Think_cloud1.png" alt="cloud1" class="think">
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Modeling/Heating">Heating</a></li>
<img src="https://static.igem.org/mediawiki/2014/5/56/Think_cloud2.png" alt="cloud2" class="think">
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Modeling/Thermosensitive_Regulator">Thermosensitive Regulator</a></li>
<img src="https://static.igem.org/mediawiki/2014/4/40/Think_cloud3.png" alt="cloud3" class="think">
+
                            </ul>
<img src="https://static.igem.org/mediawiki/2014/d/dc/Think_cloud4.png" alt="cloud4" class="think">
+
                        </li>
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+
                        <li class="dropdown1"><a class="down-scroll" href="#">HUMAN PRACTISE</a>
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                                <li><a href="https://2015.igem.org/Team:OUC-China/Practices">Overview</a></li>
<img src="https://static.igem.org/mediawiki/2014/1/11/Sysuchina_bg14.png" alt="board" id="board">
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Safety">Safety</a></li>
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+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Education">Education</a></li>
<img src="https://static.igem.org/mediawiki/2014/6/6c/M13.png" alt="boardFont" class="boardFont">
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Transportation">Transportation</a></li>
</div>
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Communication">Communication</a></li>
</div>
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Guide_Of_Logo_Design">Guide of Logo Design</a></li>
       
+
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        <div class="pipeline">
+
                        </li>
        <img src="https://static.igem.org/mediawiki/2014/6/66/Line2.png" alt="line" class="line" />      
+
                        <li class="dropdown1"><a class="down-scroll" href="#">NOTE BOOK</a>
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                            <ul class="dropdown2">
       
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Note_Book/Lab_Journal">Lab Journal</a></li>
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+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Note_Book/Protocol">Protocol</a></li>
       
+
                            </ul>
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                        <li class="dropdown1"><a class="down-scroll" href="#">TEAM</a>
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        <div class="bottom" id="bottom1">
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Attributions">Attribution</a></li>
        <img src="https://static.igem.org/mediawiki/2014/f/ff/Sysuchina_hide.png" id="hide1">
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Collaborations">Collaboration</a></li>
        <img src="https://static.igem.org/mediawiki/2014/a/aa/Sysuchina_hide2.png" id="hide2">
+
                            </ul>
        <img src="https://static.igem.org/mediawiki/2014/6/63/Sysuchina_stroll.png" class="stroll">
+
                        </li>
        <img src="https://static.igem.org/mediawiki/2014/0/00/Sysuchina_scrolldown1.png" class="down1" id="arr1">
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        <div class="intro">
+
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<p>
+
                <div class="clearfix"> </div>
We have the Integrated Evolution Machine!
+
</p>
+
 
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             <h2>Magthermo coli</h2>
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                    how does it <span>work?</span>
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                    This summer we designed Magthermo coli——a platform for remote regulation of gene expression by electromagnetic signal.
<h3>PROJECT</h3>
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                </p>
<p>brief introduction <a href="https://2014.igem.org/Team:SYSU-China/content.html#Project/Background">know more</a></p>
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                    <h3 class="text-uppercase text-center">step 1</h3>
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                    <p>Our Magthermo coli contains 2 core components: <B>Magnetic Receiver & Thermosensitive Regulator.</B></p>
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<h3>JUDGING</h3>
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<p>brief introduction <a href="https://2014.igem.org/Team:SYSU-China/content.html#Judging/Safetyform">know more</a></p>
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                    <h3 class="text-uppercase text-center">step 2</h3>
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                    <p>Inducing with electromagnetic field, magnetic receiver will heat, raising the ambient temperature.</p>
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                    <h3 class="text-uppercase text-center">step 3</h3>
<h3>HUMAN PRACTICE</h3>
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<p>brief introduction<a href="https://2014.igem.org/Team:SYSU-China/content.html#HumanPractice/iGEMCC">know more</a></p>
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                        In response to the change of temperature, thermosensitive regulator will initiate downstream gene (GFP for example) expression.
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            <h3 class="text-center index-headline">Magnetic <span>Receiver</span></h3>
<p>brief introduction <a href="https://2014.igem.org/Team:SYSU-China/content.html#Team/Ourteam">know more</a></p>
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                            For magnetic receiver,we chose <B>Ferritin</B>, the iron-storage protein in many organisms, which could synthesize ferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core will heat, raising the ambient temperature.
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<img  id="sysu" src="https://static.igem.org/mediawiki/2014/7/7b/Sysuchina_bg36.png" alt="sysu-china">
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                            In this section, we explored measurement techniques for the valuation of magnetism, biomineralization and protein concentration per cell.
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                        <p><a href="https://2015.igem.org/Team:OUC-China/Project/Magnetic_Receiver" class="btn btn-primary" role="button">More details</a></p>
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          <p>E-mail: igem.sysu.china@gmail.com </p>
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            <h3 class="text-center index-headline index-headline2">Thermosensitive <span>Regulator</span></h3>
   
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            <p class="text-center">For thermosensitive regulator,we chose <B>RNA thermometer</B> and designed a <B>thermosensitive T7 RNA polymerase.</B>  </p>
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                            <B>RNA thermometer</B> is a structured RNA which can expose SD sequences only at appropriate temperature.  In this section, we explored the measurement method to identify the efficiency of thermosensitive regulator under heat stress.
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                        <p><a href="https://2015.igem.org/Team:OUC-China/Project/Thermosensitive_Regulator" class="btn btn-primary" role="button">More details</a></p>
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                            <B>Thermosensitive T7 RNA polymerase</B> is a T7 RNA polymerase interrupted by a temperature-sensitive intein, which can self-splice at specific temperature and initiate the downstream signal.
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                        <p><a href="https://2015.igem.org/Team:OUC-China/Project/Thermosensitive_Regulator" class="btn btn-primary" role="button">More details</a></p>
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Latest revision as of 01:50, 19 September 2015

<!DOCTYPE html> Team:OUC-China

how does it work?

This summer we designed Magthermo coli——a platform for remote regulation of gene expression by electromagnetic signal.

step 1

Our Magthermo coli contains 2 core components: Magnetic Receiver & Thermosensitive Regulator.

step 2

Inducing with electromagnetic field, magnetic receiver will heat, raising the ambient temperature.

step 3

In response to the change of temperature, thermosensitive regulator will initiate downstream gene (GFP for example) expression.

Magnetic Receiver

For magnetic receiver,we chose Ferritin, the iron-storage protein in many organisms, which could synthesize ferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core will heat, raising the ambient temperature.

In this section, we explored measurement techniques for the valuation of magnetism, biomineralization and protein concentration per cell.

More details

Thermosensitive Regulator

For thermosensitive regulator,we chose RNA thermometer and designed a thermosensitive T7 RNA polymerase.

RNA thermometer is a structured RNA which can expose SD sequences only at appropriate temperature. In this section, we explored the measurement method to identify the efficiency of thermosensitive regulator under heat stress.

More details

Thermosensitive T7 RNA polymerase is a T7 RNA polymerase interrupted by a temperature-sensitive intein, which can self-splice at specific temperature and initiate the downstream signal.

More details