Difference between revisions of "Team:OUC-China"
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| − | <html lang=" | + | <!DOCTYPE html> |
| − | <title>Team:OUC-China</title> | + | <html lang="zh-CN"> |
| − | < | + | |
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| − | + | </style> | |
| + | </head> | ||
| − | + | <body> | |
| + | <header class="header"> | ||
| + | <nav class="navbar navbar-default"> | ||
| + | <div class="container"> | ||
| + | <span class="menu"> Menu</span> | ||
| + | <div class="banner-top"> | ||
| + | <ul class="nav banner-nav"> | ||
| + | <li><a href="https://2015.igem.org/Team:OUC-China">HOME</a></li> | ||
| + | <li class="dropdown1"><a class="down-scroll" href="#">PROJECT</a> | ||
| + | <ul class="dropdown2"> | ||
| + | <li><a href="https://2015.igem.org/Team:OUC-China/Project/Overview">Overview</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:OUC-China/Project/Magnetic_Receiver">Magnetic Receiver</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:OUC-China/Project/Thermosensitive_Regulator">Thermosensitive Regulator</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:OUC-China/Project/Captor">Captor</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:OUC-China/Project/Results">Results</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:OUC-China/Parts">Parts</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:OUC-China/Project/Achievements">Achievements</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li class="dropdown1"><a href="https://2015.igem.org/Team:OUC-China/Interlab">INTERLAB</a> | ||
| + | </li> | ||
| + | <li class="dropdown1"><a class="down-scroll" href="#">MODELING</a> | ||
| + | <ul class="dropdown2"> | ||
| + | <li><a href="https://2015.igem.org/Team:OUC-China/Modeling">Overview</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:OUC-China/Modeling/Magnetic_Receiver">Magnetic Receiver</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:OUC-China/Modeling/Heating">Heating</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:OUC-China/Modeling/Thermosensitive_Regulator">Thermosensitive Regulator</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li class="dropdown1"><a class="down-scroll" href="#">HUMAN PRACTISE</a> | ||
| + | <ul class="dropdown2"> | ||
| + | <li><a href="https://2015.igem.org/Team:OUC-China/Practices">Overview</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Safety">Safety</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Education">Education</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Transportation">Transportation</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Communication">Communication</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Guide_Of_Logo_Design">Guide of Logo Design</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li class="dropdown1"><a class="down-scroll" href="#">NOTE BOOK</a> | ||
| + | <ul class="dropdown2"> | ||
| + | <li><a href="https://2015.igem.org/Team:OUC-China/Note_Book/Lab_Journal">Lab Journal</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:OUC-China/Note_Book/Protocol">Protocol</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li class="dropdown1"><a class="down-scroll" href="#">TEAM</a> | ||
| + | <ul class="dropdown2"> | ||
| + | <li><a href="https://2015.igem.org/Team:OUC-China/Team/Member">Member</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:OUC-China/Attributions">Attribution</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:OUC-China/Collaborations">Collaboration</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | </ul> | ||
| + | <script> | ||
| + | $("span.menu").click(function() { | ||
| + | $(" ul.nav").slideToggle("slow", function() {}); | ||
| + | }); | ||
| + | </script> | ||
| + | </div> | ||
| + | <div class="clearfix"> </div> | ||
| + | </div> | ||
| + | <!-- /.container-fluid --> | ||
| + | </nav> | ||
| + | </header> | ||
| + | <div class="banner-container"> | ||
| + | <div class="banner-content"> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/4/4b/OUC-China-Team_Logo.png" width="400" alt="" class="img-responsive center-block"> | ||
| + | <h2>Magthermo coli</h2> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="container-fluid how-work"> | ||
| + | <div class="container"> | ||
| + | <div class="work-header"> | ||
| + | <h2 class="text-uppercase"> | ||
| + | how does it <span>work?</span> | ||
| + | </h2> | ||
| + | <p> | ||
| + | This summer we designed Magthermo coli——a platform for remote regulation of gene expression by electromagnetic signal. | ||
| + | </p> | ||
| + | </div> | ||
| + | <div class="row work-content"> | ||
| + | <div class="col-md-4"> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/e/e1/OUC-China-home_receiver_regulator1.png" alt="" class="img-responsive"> | ||
| + | <h3 class="text-uppercase text-center">step 1</h3> | ||
| + | <p>Our Magthermo coli contains 2 core components: <B>Magnetic Receiver & Thermosensitive Regulator.</B></p> | ||
| + | </div> | ||
| + | <div class="col-md-4"> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/e/e7/OUC-China-home_receiver_regulator2.png" alt="" class="img-responsive"> | ||
| + | <h3 class="text-uppercase text-center">step 2</h3> | ||
| + | <p>Inducing with electromagnetic field, magnetic receiver will heat, raising the ambient temperature.</p> | ||
| + | </div> | ||
| + | <div class="col-md-4"> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/6/6d/OUC-China_home_receiver_regulator3.png" alt="" class="img-responsive"> | ||
| + | <h3 class="text-uppercase text-center">step 3</h3> | ||
| + | <p> | ||
| + | In response to the change of temperature, thermosensitive regulator will initiate downstream gene (GFP for example) expression. | ||
| + | </p> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="container-fluid index-details1 index-details"> | ||
| + | <div class="container"> | ||
| + | <h3 class="text-center index-headline">Magnetic <span>Receiver</span></h3> | ||
| + | <div class="row"> | ||
| + | <div class="col-md-7"> | ||
| + | <div class="caption"> | ||
| + | <p> | ||
| + | For magnetic receiver,we chose <B>Ferritin</B>, the iron-storage protein in many organisms, which could synthesize ferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core will heat, raising the ambient temperature. | ||
| + | </p> | ||
| + | <p> | ||
| + | In this section, we explored measurement techniques for the valuation of magnetism, biomineralization and protein concentration per cell. | ||
| + | </p> | ||
| + | <p><a href="https://2015.igem.org/Team:OUC-China/Project/Magnetic_Receiver" class="btn btn-primary" role="button">More details</a></p> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="col-md-5"> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/1/17/OUC-China-home-frritin.png" alt="" class="img-responsive"> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="container-fluid index-details2 index-details"> | ||
| + | <div class="container"> | ||
| + | <h3 class="text-center index-headline index-headline2">Thermosensitive <span>Regulator</span></h3> | ||
| + | <p class="text-center">For thermosensitive regulator，we chose <B>RNA thermometer</B> and designed a <B>thermosensitive T7 RNA polymerase.</B> </p> | ||
| + | <div class="row"> | ||
| + | <div class="col-md-6"> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/b/b8/OUC-China-home-RNA.png" alt="" class="img-responsive"> | ||
| + | </div> | ||
| + | <div class="col-md-6"> | ||
| + | <div class="caption"> | ||
| + | <p> | ||
| + | <B>RNA thermometer</B> is a structured RNA which can expose SD sequences only at appropriate temperature. In this section, we explored the measurement method to identify the efficiency of thermosensitive regulator under heat stress. | ||
| + | </p> | ||
| + | <p><a href="https://2015.igem.org/Team:OUC-China/Project/Thermosensitive_Regulator" class="btn btn-primary" role="button">More details</a></p> | ||
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| + | </div> | ||
| + | <div class="row"> | ||
| + | <div class="col-md-6"> | ||
| + | <div class="caption"> | ||
| + | <p> | ||
| + | <B>Thermosensitive T7 RNA polymerase</B> is a T7 RNA polymerase interrupted by a temperature-sensitive intein, which can self-splice at specific temperature and initiate the downstream signal. | ||
| + | </p> | ||
| + | <p><a href="https://2015.igem.org/Team:OUC-China/Project/Thermosensitive_Regulator" class="btn btn-primary" role="button">More details</a></p> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="col-md-6"> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/4/4d/OUC-China-home-T7.png" alt="" class="img-responsive"> | ||
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Latest revision as of 01:50, 19 September 2015
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Magthermo coli
how does it work?
This summer we designed Magthermo coli——a platform for remote regulation of gene expression by electromagnetic signal.
step 1
Our Magthermo coli contains 2 core components: Magnetic Receiver & Thermosensitive Regulator.
step 2
Inducing with electromagnetic field, magnetic receiver will heat, raising the ambient temperature.
step 3
In response to the change of temperature, thermosensitive regulator will initiate downstream gene (GFP for example) expression.
Magnetic Receiver
For magnetic receiver,we chose Ferritin, the iron-storage protein in many organisms, which could synthesize ferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core will heat, raising the ambient temperature.
In this section, we explored measurement techniques for the valuation of magnetism, biomineralization and protein concentration per cell.
Thermosensitive Regulator
For thermosensitive regulator，we chose RNA thermometer and designed a thermosensitive T7 RNA polymerase.
RNA thermometer is a structured RNA which can expose SD sequences only at appropriate temperature. In this section, we explored the measurement method to identify the efficiency of thermosensitive regulator under heat stress.
Thermosensitive T7 RNA polymerase is a T7 RNA polymerase interrupted by a temperature-sensitive intein, which can self-splice at specific temperature and initiate the downstream signal.
