Difference between revisions of "Team:Harvard BioDesign/Notebook"

 
(9 intermediate revisions by 2 users not shown)
Line 4: Line 4:
 
   Free for personal and commercial use under the CCA 3.0 license (html5up.net/license)
 
   Free for personal and commercial use under the CCA 3.0 license (html5up.net/license)
 
-->
 
-->
−
 
 
<html>
 
<html>
−
  <head>
+
<head>
−
    <title>Prologue by HTML5 UP</title>
+
  <title>Prologue by HTML5 UP</title>
−
    <meta charset="utf-8" />
+
  <meta charset="utf-8" />
−
    <meta name="viewport" content="width=device-width, initial-scale=1" />
+
  <meta name="viewport" content="width=device-width, initial-scale=1" />
−
    <!--[if lte IE 8]><script src="assets/js/ie/html5shiv.js"></script><![endif]-->
+
  <!--[if lte IE 8]><script src="assets/js/ie/html5shiv.js"></script><![endif]-->
−
    <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Harvard_BioDesign/CSS_Scroll0?action=raw&ctype=text/css" />
+
  <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Harvard_BioDesign/CSS_Scroll0?action=raw&ctype=text/css" />
−
    <!--[if lte IE 8]><link rel="stylesheet" href="assets/css/ie8.css" /><![endif]-->
+
  <!--[if lte IE 8]><link rel="stylesheet" href="assets/css/ie8.css" /><![endif]-->
−
    <!--[if lte IE 9]><link rel="stylesheet" href="assets/css/ie9.css" /><![endif]-->
+
  <!--[if lte IE 9]><link rel="stylesheet" href="assets/css/ie9.css" /><![endif]-->
  
  
−
  </head>
+
</head>
−
  <body>
+
  
 +
<body>
  
−
    <!-- header -->
 
−
      <div id="header">
 
−
        <div class="inner">
 
−
        <div class="top">
 
−
          <!-- Logo -->
 
−
            <div id="logo">
 
−
              <span class="harvardLogo"><a href="https://2015.igem.org/Team:Harvard_BioDesign"><img src="https://static.igem.org/mediawiki/2015/9/94/HarvardBioDesign2015Logo2.png"alt="Harvard Logo" style="width:212px;height:144px;"/></a>  </span>
 
−
            <!-- <h1 id="title">Harvard BioDesign 2015</h1>-->
 
−
            </div><!-- #Logo -->
 
  
−
          <!-- Nav -->
+
  <!-- header -->
−
             <nav id="nav">
+
    <div id="header">
−
              <!--
+
      <div class="inner">
 +
      <div class="top">
 +
        <!-- Logo -->
 +
          <div id="logo">
 +
             <span class="harvardLogo"><a href="https://2015.igem.org/Team:Harvard_BioDesign"><img src="https://static.igem.org/mediawiki/2015/9/94/HarvardBioDesign2015Logo2.png"alt="Harvard Logo" style="width:275px;height:200px;margin-left:-50px"/></a> </span>
 +
          <!-- <h1 id="title">Harvard BioDesign 2015</h1>-->
 +
          </div><!-- #Logo -->
  
−
                Prologue's nav expects links in one of two formats:
+
        <!-- Nav -->
 +
          <nav id="nav">
 +
            <!--
  
−
                1. Hash link (scrolls to a different section within the page)
+
              Prologue's nav expects links in one of two formats:
  
−
                  <li><a href="#foobar" id="foobar-link" class="icon fa-whatever-icon-you-want skel-layers-ignoreHref"><span class="label">Foobar</span></a></li>
+
              1. Hash link (scrolls to a different section within the page)
  
−
                2. Standard link (sends the user to another page/site)
+
                <li><a href="#foobar" id="foobar-link" class="icon fa-whatever-icon-you-want skel-layers-ignoreHref"><span class="label">Foobar</span></a></li>
  
−
                  <li><a href="http://foobar.tld" id="foobar-link" class="icon fa-whatever-icon-you-want"><span class="label">Foobar</span></a></li>
+
              2. Standard link (sends the user to another page/site)
  
−
              -->
+
                <li><a href="http://foobar.tld" id="foobar-link" class="icon fa-whatever-icon-you-want"><span class="label">Foobar</span></a></li>
−
          <!-- Subsections -->
+
 
 +
            -->
 +
        <!-- Subsections -->
 
</nav>
 
</nav>
−
</div><!--#top-->      
+
</div><!--#top-->
  
−
        <div class="bottom">
+
      <div class="bottom">
−
<nav id="nav"> <!-- Main Menu --> <h4><div class="innertwo">Main Pages</div></h4>  
+
<nav id="nav"> <!-- Main Menu --> <h4><div class="innertwo">Main Pages</div></h4>
−
<ul>  
+
  <ul>
−
<li><a href="https://2015.igem.org/Team:Harvard_BioDesign">Home</a></li>  
+
  <li><a href="https://2015.igem.org/Team:Harvard_BioDesign">Home</a></li>
−
<li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Project">Project</a></li>  
+
  <li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Project">Project</a></li>
−
<li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Results">Results</a></li>  
+
  <li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Results">Results</a></li>
−
<li><a class="curPage" href="https://2015.igem.org/Team:Harvard_BioDesign/Notebook">Notebook</a></li>  
+
  <li><a class="curPage" href="https://2015.igem.org/Team:Harvard_BioDesign/Notebook">Notebook</a></li>
−
<li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Parts">Parts</a></li>  
+
  <li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Parts">Parts</a></li>
−
<li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Practices">Practices</a></li>  
+
  <li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Practices">Practices</a></li>
−
<li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Safety">Safety</a></li>  
+
  <li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Safety">Safety</a></li>
−
<li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Collaborations">Collaborations</a></li>
+
  <li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Collaborations">Collaborations</a></li>
−
<li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Team">Team</a></li>
+
  <li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Team">Team</a></li>
−
<li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Attributions">Attributions</a></li>  
+
  <li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Attributions">Attributions</a></li>
−
</ul>
+
  </ul>
−
</nav>
+
</nav>
  
  
  
−
          <!-- Social Icons -->
+
        <!-- Social Icons -->
−
            <ul class="icons">
+
          <ul class="icons">
  
−
              <li><a href="https://www.facebook.com/harvardigem2015" target="_blank">
+
            <li><a href="https://www.facebook.com/harvardigem2015" target="_blank">
−
                    <img src="https://static.igem.org/mediawiki/2015/e/ed/Harvard2015Facebookwhite.png"alt="Facebook Logo" style="width:51px;height:51px;"></a></li>
+
                  <img src="https://static.igem.org/mediawiki/2015/e/ed/Harvard2015Facebookwhite.png"alt="Facebook Logo" style="width:51px;height:51px;"></a></li>
−
              <li><a href="https://twitter.com/HarvardiGEM2015" target="_blank">
+
            <li><a href="https://twitter.com/HarvardiGEM2015" target="_blank">
−
                    <img src="https://static.igem.org/mediawiki/2015/6/64/Harvard2015Twitterlogowhite.png"alt="Twitter Logo" style="width:51px;height:51px;"></a></li>
+
                  <img src="https://static.igem.org/mediawiki/2015/6/64/Harvard2015Twitterlogowhite.png"alt="Twitter Logo" style="width:51px;height:51px;"></a></li>
−
              <li><a href="mailto:harvardigem2015@gmail.com">
+
            <li><a href="mailto:harvardigem2015@gmail.com">
−
                    <img src="https://static.igem.org/mediawiki/2015/9/98/Harvard2015Emaillogowhite.png" alt="Email Logo" style="width:51px;height:51px;"></a></li>  
+
                  <img src="https://static.igem.org/mediawiki/2015/9/98/Harvard2015Emaillogowhite.png" alt="Email Logo" style="width:51px;height:51px;"></a></li>
−
            </ul>
+
          </ul>
−
        </div><!--#bottom-->
+
      </div><!--#bottom-->
−
        </div><!--#inner-->
+
      </div><!--#inner-->
−
      </div><!--#header-->
+
    </div><!--#header-->
  
 
     <!-- Main -->
 
     <!-- Main -->
Line 89: Line 89:
  
 
               <header>
 
               <header>
−
<img src="https://static.igem.org/mediawiki/2015/5/53/Harvard2015_Notebook.png"/>
+
    <img src="https://static.igem.org/mediawiki/2015/5/53/Harvard2015_Notebook.png"/>
−
                 <h2 class="alt">Our 2015 <strong>Lab Notebook</strong>!<br /></h2>
+
                 <h2 class="alt">Our <strong>2015 Lab Notebook</strong>!<br /></h2>
−
                <p>...Will be updated at a later time when we have pieced our sanity back together.</p>
+
 
               </header>
 
               </header>
  
−
            </div>
+
<div class="inner-container" >
−
          </section>
+
  
 +
<div class="noterow">
 +
<div class = "column">
  
  
−
      </div>
+
<div class="entry"><img style="width:100%;" src="https://static.igem.org/mediawiki/2015/f/f8/Harvard_Notebook_1.jpg" /></div>
  
−
    <!-- Footer -->
+
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 6/1</h2>
−
      <div id="footer">
+
<p class="entrytext">First week in lab!
 +
  <br>Microbiology boot camp.
 +
  <br>How to pour plates,
 +
  <br>PCR, miniprep, gel electrophoresis.
 +
  <br>
 +
Researched project ideas.</p></div>
  
−
      <ul class="copyright">
+
<br><br>
−
          <li><a href="https://static.igem.org/mediawiki/2015/c/ce/Harvard2015SiteMap.png" target="_blank">Site Map</a></li><li>Site template designed by: <a href="http://html5up.net">HTML5 UP</a></li>
+
 
−
          </ul>
+
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 6/8</h2>
 +
<p class="entrytext">Settle on bacterial adhesion.
 +
<br>
 +
Further background research.
 +
<br>
 +
Plasmid design, primer design.
 +
<br>
 +
Chose fusion peptides.
 +
<br>
 +
Ordered gBlocks from IDT of Rhamnose, Lac, and Arabinose promoters.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 6/15</h2>
 +
<p class="entrytext">PCR backbones and fim proteins.
 +
  <br>
 +
Poured Chloramphenicol and Ampicillin plates.
 +
<br>
 +
Gibson assembly of operon plasmid and fimH plasmid.
 +
<br>
 +
Pili purification assay development.
 +
<br>
 +
NEGEM Meetup #1.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 6/22</h2>
 +
<p class="entrytext">Redo failed PCRs.
 +
  <br>
 +
Redo failed Gibsons.
 +
<br>
 +
Transform and plate operon Gibson.
 +
<br>
 +
Pili purification and agglutination assay development.
 +
<br>
 +
Microscopy on control strains.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 6/29</h2>
 +
<p class="entrytext">Miniprep operon plasmid.
 +
  <br>
 +
Sent for sequencing.
 +
<br>
 +
Unsuccessful- redo operon PCRs.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 7/6</h2>
 +
<p class="entrytext">Induction.
 +
  <br>
 +
Operon Gibson.
 +
<br>
 +
Agglutination assay development.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 7/13</h2>
 +
<p class="entrytext">Induction.
 +
  <br>
 +
Edit out illegal PstI cut site from fimH.
 +
<br>
 +
Mutagenesis to add binding peptides.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 7/20</h2>
 +
<p class="entrytext">Induction.
 +
  <br>
 +
Transform measurement study parts.
 +
<br>
 +
Mutagenesis to add binding peptides.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 7/27</h2>
 +
<p class="entrytext">Induction.
 +
  <br>
 +
Nickel binding assay.
 +
<br>
 +
Made biobricks for pRha-fimH and fimH. PCR-ing out from parts we were using with low-copy backbone, Gibson with pSB1C3.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 8/2</h2>
 +
<p class="entrytext">Induction. Induction. More Induction.
 +
  <br>
 +
Nickel binding assay.
 +
<br>
 +
Stainless steel binding assay.
 +
<br>
 +
Cancer binding assay.
 +
<br>
 +
Made biobricks, sent for sequencing.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
 
 +
<div class="entry"><img style="width:100%;" src="https://static.igem.org/mediawiki/2015/e/e7/Harvard_Notebook_2.jpg" /></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week 8/9</h2>
 +
<p class="entrytext">Induction. Induction. More Induction.
 +
Nickel binding assay.
 +
Stainless steel binding assay.
 +
Cancer binding assay.
 +
Retried failed biobricks.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 8/16</h2>
 +
<p class="entrytext">Break from lab.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 8/23</h2>
 +
<p class="entrytext">Break from lab.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 8/30</h2>
 +
<p class="entrytext">Wiki and presentation planning.
 +
</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 9/6</h2>
 +
<p class="entrytext">Retried failed biobricks.
 +
 
 +
Obtained results for Rhamnose-GFP inductions.
 +
 
 +
Made presentation.
 +
 
 +
NEGEM Meetup #2!</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 9/13</h2>
 +
<p class="entrytext">Worked on parts pages.
 +
Worked on wiki.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">9/24 - 9/28</h2>
 +
<p class="entrytext">iGEM Giant Jamboree!!!</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img style="width:100%;" src="https://static.igem.org/mediawiki/2015/7/79/Harvard_Notebook_3.jpg" /></div>
 +
</div>
 +
</div>
 +
</div> <!--Intro-->
 +
</section>
 +
 
 +
<section id="notes" class="two">
 +
<div class="container">
 +
 
 +
</div></section>
 +
 
 +
<!-- Footer -->
 +
  <div id="footer">
 +
 
 +
  <ul class="copyright">
 +
      <li>Site template designed by: <a href="http://html5up.net">HTML5 UP</a></li>
 +
      </ul>
 +
 
 +
  </div> <!--Footer-->
 +
</div> <!--Main-->
  
−
      </div>
+
<!-- Scripts -->
 +
  <script type="text/javascript" src="https://2015.igem.org/Template:Harvard_BioDesign/JS_Scroll1?action=raw&ctype=text/javascript"></script>
 +
  <script type="text/javascript" src="https://2015.igem.org/Template:Harvard_BioDesign/JS_Scroll2?action=raw&ctype=text/javascript"></script>
 +
  <script type="text/javascript" src="https://2015.igem.org/Template:Harvard_BioDesign/JS_Scroll3?action=raw&ctype=text/javascript"></script>
 +
  <script type="text/javascript" src="https://2015.igem.org/Template:Harvard_BioDesign/JS_Scroll4?action=raw&ctype=text/javascript"></script>
 +
  <script type="text/javascript" src="https://2015.igem.org/Template:Harvard_BioDesign/JS_Scroll5?action=raw&ctype=text/javascript"></script>
 +
  <!--[if lte IE 8]><script src="assets/js/ie/respond.min.js"></script><![endif]-->
 +
  <script type="text/javascript" src="https://2015.igem.org/Template:Harvard_BioDesign/JS_Scroll0?action=raw&ctype=text/javascript"></script>
  
−
    <!-- Scripts -->
 
−
      <script type="text/javascript" src="https://2015.igem.org/Template:Harvard_BioDesign/JS_Scroll1?action=raw&ctype=text/javascript"></script>
 
−
      <script type="text/javascript" src="https://2015.igem.org/Template:Harvard_BioDesign/JS_Scroll2?action=raw&ctype=text/javascript"></script>
 
−
      <script type="text/javascript" src="https://2015.igem.org/Template:Harvard_BioDesign/JS_Scroll3?action=raw&ctype=text/javascript"></script>
 
−
      <script type="text/javascript" src="https://2015.igem.org/Template:Harvard_BioDesign/JS_Scroll4?action=raw&ctype=text/javascript"></script>
 
−
      <script type="text/javascript" src="https://2015.igem.org/Template:Harvard_BioDesign/JS_Scroll5?action=raw&ctype=text/javascript"></script>
 
−
      <!--[if lte IE 8]><script src="assets/js/ie/respond.min.js"></script><![endif]-->
 
−
      <script type="text/javascript" src="https://2015.igem.org/Template:Harvard_BioDesign/JS_Scroll0?action=raw&ctype=text/javascript"></script>
 
  
−
  </body>
+
</body>
 
</html>
 
</html>

Latest revision as of 01:54, 19 September 2015

Prologue by HTML5 UP

Our 2015 Lab Notebook!

Week of 6/1

First week in lab!
Microbiology boot camp.
How to pour plates,
PCR, miniprep, gel electrophoresis.
Researched project ideas.



Week of 6/8

Settle on bacterial adhesion.
Further background research.
Plasmid design, primer design.
Chose fusion peptides.
Ordered gBlocks from IDT of Rhamnose, Lac, and Arabinose promoters.



Week of 6/15

PCR backbones and fim proteins.
Poured Chloramphenicol and Ampicillin plates.
Gibson assembly of operon plasmid and fimH plasmid.
Pili purification assay development.
NEGEM Meetup #1.



Week of 6/22

Redo failed PCRs.
Redo failed Gibsons.
Transform and plate operon Gibson.
Pili purification and agglutination assay development.
Microscopy on control strains.



Week of 6/29

Miniprep operon plasmid.
Sent for sequencing.
Unsuccessful- redo operon PCRs.



Week of 7/6

Induction.
Operon Gibson.
Agglutination assay development.



Week of 7/13

Induction.
Edit out illegal PstI cut site from fimH.
Mutagenesis to add binding peptides.



Week of 7/20

Induction.
Transform measurement study parts.
Mutagenesis to add binding peptides.



Week of 7/27

Induction.
Nickel binding assay.
Made biobricks for pRha-fimH and fimH. PCR-ing out from parts we were using with low-copy backbone, Gibson with pSB1C3.



Week of 8/2

Induction. Induction. More Induction.
Nickel binding assay.
Stainless steel binding assay.
Cancer binding assay.
Made biobricks, sent for sequencing.





Week 8/9

Induction. Induction. More Induction. Nickel binding assay. Stainless steel binding assay. Cancer binding assay. Retried failed biobricks.



Week of 8/16

Break from lab.



Week of 8/23

Break from lab.



Week of 8/30

Wiki and presentation planning.



Week of 9/6

Retried failed biobricks. Obtained results for Rhamnose-GFP inductions. Made presentation. NEGEM Meetup #2!



Week of 9/13

Worked on parts pages. Worked on wiki.



9/24 - 9/28

iGEM Giant Jamboree!!!