Difference between revisions of "Team:Harvard BioDesign/Notebook"

 
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<div class="inner-container" >
 
<div class="inner-container" >
  
 +
<div class="noterow">
 +
<div class = "column">
  
−
<!-- JOURNAL (not mobiles) -->
 
  
−
<div class="noterow" id="nonmobilejournal">
+
<div class="entry"><img style="width:100%;" src="https://static.igem.org/mediawiki/2015/f/f8/Harvard_Notebook_1.jpg" /></div>
−
<div class = "col-md-12">
+
  
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 6/1</h2>
 +
<p class="entrytext">First week in lab!
 +
  <br>Microbiology boot camp.
 +
  <br>How to pour plates,
 +
  <br>PCR, miniprep, gel electrophoresis.
 +
  <br>
 +
Researched project ideas.</p></div>
  
−
<div class="entry"><img style="width:100%;" src="https://static.igem.org/mediawiki/2015/a/a3/Aalto-Helsinki_collage_9.jpg" /></div>
+
<br><br>
  
−
<!-- June below -->
+
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 6/8</h2>
−
 
+
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">Week of 6/1</h2>
+
−
<p class="entrytext">First week in lab! Microbiology boot camp. How to pour plates, PCR, miniprep, gel electrophoresis.
+
−
Researching project ideas.</p></div>
+
−
 
+
−
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">Week of 6/8</h2>
+
 
<p class="entrytext">Settle on bacterial adhesion.
 
<p class="entrytext">Settle on bacterial adhesion.
 +
<br>
 
Further background research.
 
Further background research.
 +
<br>
 
Plasmid design, primer design.
 
Plasmid design, primer design.
−
Choose fusion peptides.
+
<br>
−
Order gBlocks from IDT of Rhamnose, Lac, and Arabinose promoters.</p></div>
+
Chose fusion peptides.
 +
<br>
 +
Ordered gBlocks from IDT of Rhamnose, Lac, and Arabinose promoters.</p></div>
  
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">Week of 6/15</h2>
+
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 6/15</h2>
 
<p class="entrytext">PCR backbones and fim proteins.
 
<p class="entrytext">PCR backbones and fim proteins.
−
Pour Chlor and Amp plates.
+
  <br>
 +
Poured Chloramphenicol and Ampicillin plates.
 +
<br>
 
Gibson assembly of operon plasmid and fimH plasmid.
 
Gibson assembly of operon plasmid and fimH plasmid.
 +
<br>
 
Pili purification assay development.
 
Pili purification assay development.
 +
<br>
 
NEGEM Meetup #1.</p></div>
 
NEGEM Meetup #1.</p></div>
  
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">Week of 6/22</h2>
+
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 6/22</h2>
 
<p class="entrytext">Redo failed PCRs.
 
<p class="entrytext">Redo failed PCRs.
 +
  <br>
 
Redo failed Gibsons.
 
Redo failed Gibsons.
 +
<br>
 
Transform and plate operon Gibson.
 
Transform and plate operon Gibson.
 +
<br>
 
Pili purification and agglutination assay development.
 
Pili purification and agglutination assay development.
 +
<br>
 
Microscopy on control strains.</p></div>
 
Microscopy on control strains.</p></div>
  
−
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">Week of 6/29</h2>
+
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 6/29</h2>
 
<p class="entrytext">Miniprep operon plasmid.
 
<p class="entrytext">Miniprep operon plasmid.
 +
  <br>
 
Sent for sequencing.
 
Sent for sequencing.
 +
<br>
 
Unsuccessful- redo operon PCRs.</p></div>
 
Unsuccessful- redo operon PCRs.</p></div>
  
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">Week of 7/6</h2>
+
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 7/6</h2>
 
<p class="entrytext">Induction.
 
<p class="entrytext">Induction.
 +
  <br>
 
Operon Gibson.
 
Operon Gibson.
 +
<br>
 
Agglutination assay development.</p></div>
 
Agglutination assay development.</p></div>
  
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">Week of 7/13</h2>
+
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 7/13</h2>
 
<p class="entrytext">Induction.
 
<p class="entrytext">Induction.
 +
  <br>
 
Edit out illegal PstI cut site from fimH.
 
Edit out illegal PstI cut site from fimH.
 +
<br>
 
Mutagenesis to add binding peptides.</p></div>
 
Mutagenesis to add binding peptides.</p></div>
  
−
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">Week of 7/20</h2>
+
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 7/20</h2>
 
<p class="entrytext">Induction.
 
<p class="entrytext">Induction.
 +
  <br>
 
Transform measurement study parts.
 
Transform measurement study parts.
 +
<br>
 
Mutagenesis to add binding peptides.</p></div>
 
Mutagenesis to add binding peptides.</p></div>
  
−
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">Week of 7/27</h2>
+
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 7/27</h2>
 
<p class="entrytext">Induction.
 
<p class="entrytext">Induction.
 +
  <br>
 
Nickel binding assay.
 
Nickel binding assay.
 +
<br>
 
Made biobricks for pRha-fimH and fimH. PCR-ing out from parts we were using with low-copy backbone, Gibson with pSB1C3.</p></div>
 
Made biobricks for pRha-fimH and fimH. PCR-ing out from parts we were using with low-copy backbone, Gibson with pSB1C3.</p></div>
  
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">Week of 8/2</h2>
+
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 8/2</h2>
 
<p class="entrytext">Induction. Induction. More Induction.
 
<p class="entrytext">Induction. Induction. More Induction.
 +
  <br>
 
Nickel binding assay.
 
Nickel binding assay.
 +
<br>
 
Stainless steel binding assay.
 
Stainless steel binding assay.
 +
<br>
 
Cancer binding assay.
 
Cancer binding assay.
 +
<br>
 
Made biobricks, sent for sequencing.</p></div>
 
Made biobricks, sent for sequencing.</p></div>
  
 +
<br>
 +
<br>
  
−
<div class="entry"><img style="width:100%;" src="https://static.igem.org/mediawiki/2015/5/5b/Aalto-Helsinki_collage_8.jpg" /></div>
 
  
−
<div class="month" ><img class="monthlogo" src ="https://static.igem.org/mediawiki/2015/4/45/Aalto-Helsinki_timeline_SEP.png"></img></div>
+
<div class="entry"><img style="width:100%;" src="https://static.igem.org/mediawiki/2015/e/e7/Harvard_Notebook_2.jpg" /></div>
−
<!-- September above -->
+
  
 +
<br>
 +
<br>
  
−
<!-- August below -->
+
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week 8/9</h2>
−
 
+
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">Week 8/9</h2>
+
 
<p class="entrytext">Induction. Induction. More Induction.
 
<p class="entrytext">Induction. Induction. More Induction.
 
Nickel binding assay.
 
Nickel binding assay.
Line 179: Line 230:
 
Retried failed biobricks.</p></div>
 
Retried failed biobricks.</p></div>
  
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">Week of 8/16</h2>
+
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 8/16</h2>
 
<p class="entrytext">Break from lab.</p></div>
 
<p class="entrytext">Break from lab.</p></div>
  
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">Week of 8/23</h2>
+
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 8/23</h2>
 
<p class="entrytext">Break from lab.</p></div>
 
<p class="entrytext">Break from lab.</p></div>
  
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">Week of 8/30</h2>
+
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 8/30</h2>
 
<p class="entrytext">Wiki and presentation planning.
 
<p class="entrytext">Wiki and presentation planning.
 
</p></div>
 
</p></div>
  
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">Week of 9/6</h2>
+
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 9/6</h2>
 
<p class="entrytext">Retried failed biobricks.
 
<p class="entrytext">Retried failed biobricks.
  
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NEGEM Meetup #2!</p></div>
 
NEGEM Meetup #2!</p></div>
  
−
<div class="entry"><img class="entrylogobig" ></img><h2 class="day">Week of 9/13</h2>
+
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 9/13</h2>
 
<p class="entrytext">Worked on parts pages.
 
<p class="entrytext">Worked on parts pages.
 
Worked on wiki.</p></div>
 
Worked on wiki.</p></div>
  
−
<div class="entry"><img class="entrylogo" ></img><h2 class="day">9/24 - 9/28</h2>
+
<br>
−
<p class="entrytext">iGEM Giant Jamboree!!!</p></div>
+
<br>
  
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">9/24 - 9/28</h2>
 +
<p class="entrytext">iGEM Giant Jamboree!!!</p></div>
  
−
<div class="entry"><img style="width:100%;" src="https://static.igem.org/mediawiki/2015/5/59/Aalto-Helsinki_collage_7.jpg" /></div>
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<br>
  
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+
<div class="entry"><img style="width:100%;" src="https://static.igem.org/mediawiki/2015/7/79/Harvard_Notebook_3.jpg" /></div>
 
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Latest revision as of 01:54, 19 September 2015

Prologue by HTML5 UP

Our 2015 Lab Notebook!

Week of 6/1

First week in lab!
Microbiology boot camp.
How to pour plates,
PCR, miniprep, gel electrophoresis.
Researched project ideas.



Week of 6/8

Settle on bacterial adhesion.
Further background research.
Plasmid design, primer design.
Chose fusion peptides.
Ordered gBlocks from IDT of Rhamnose, Lac, and Arabinose promoters.



Week of 6/15

PCR backbones and fim proteins.
Poured Chloramphenicol and Ampicillin plates.
Gibson assembly of operon plasmid and fimH plasmid.
Pili purification assay development.
NEGEM Meetup #1.



Week of 6/22

Redo failed PCRs.
Redo failed Gibsons.
Transform and plate operon Gibson.
Pili purification and agglutination assay development.
Microscopy on control strains.



Week of 6/29

Miniprep operon plasmid.
Sent for sequencing.
Unsuccessful- redo operon PCRs.



Week of 7/6

Induction.
Operon Gibson.
Agglutination assay development.



Week of 7/13

Induction.
Edit out illegal PstI cut site from fimH.
Mutagenesis to add binding peptides.



Week of 7/20

Induction.
Transform measurement study parts.
Mutagenesis to add binding peptides.



Week of 7/27

Induction.
Nickel binding assay.
Made biobricks for pRha-fimH and fimH. PCR-ing out from parts we were using with low-copy backbone, Gibson with pSB1C3.



Week of 8/2

Induction. Induction. More Induction.
Nickel binding assay.
Stainless steel binding assay.
Cancer binding assay.
Made biobricks, sent for sequencing.





Week 8/9

Induction. Induction. More Induction. Nickel binding assay. Stainless steel binding assay. Cancer binding assay. Retried failed biobricks.



Week of 8/16

Break from lab.



Week of 8/23

Break from lab.



Week of 8/30

Wiki and presentation planning.



Week of 9/6

Retried failed biobricks. Obtained results for Rhamnose-GFP inductions. Made presentation. NEGEM Meetup #2!



Week of 9/13

Worked on parts pages. Worked on wiki.



9/24 - 9/28

iGEM Giant Jamboree!!!