Difference between revisions of "Team:Harvard BioDesign/Notebook"

 
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              <span class="harvardLogo"><a href="https://2015.igem.org/Team:Harvard_BioDesign" target="_blank"><img src="https://static.igem.org/mediawiki/2015/9/94/HarvardBioDesign2015Logo2.png"alt="Harvard Logo" style="width:212px;height:144px;"/></a>  </span>
 
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            <!-- <h1 id="title">Harvard BioDesign 2015</h1>-->
 
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              <p>NAME OF PROJECT</p>
 
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             <span class="harvardLogo"><a href="https://2015.igem.org/Team:Harvard_BioDesign"><img src="https://static.igem.org/mediawiki/2015/9/94/HarvardBioDesign2015Logo2.png"alt="Harvard Logo" style="width:275px;height:200px;margin-left:-50px"/></a> </span>
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          <!-- <h1 id="title">Harvard BioDesign 2015</h1>-->
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          </div><!-- #Logo -->
  
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                Prologue's nav expects links in one of two formats:
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<h4> Content </h4>
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                <li><a href="#top" id="top-link" class="skel-layers-ignoreHref"><span class="icon fa-home">Welcome</span></a></li>
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        <h4> Main Pages</h4>
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  <li><a href="https://2015.igem.org/Team:Harvard_BioDesign">Home</a></li>
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            <ul>
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  <li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Project">Project</a></li>
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                <li><a href="https://2015.igem.org/Team:Harvard_BioDesign">Home</a></li>
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  <li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Results">Results</a></li>
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                <li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Team">Team</a></li>
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  <li><a class="curPage" href="https://2015.igem.org/Team:Harvard_BioDesign/Notebook">Notebook</a></li>
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                <li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Project">Project</a></li>
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                <li><a class="curPage" href="https://2015.igem.org/Team:Harvard_BioDesign/Notebook">Notebook</a></li>
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  <li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Safety">Safety</a></li>
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                <li><a href="https://2015.igem.org/Team:Harvard_BioDesign/Collaborations">Collaborations</a></li>
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               <header>
 
               <header>
−
                 <h2 class="alt">Our 2015 <strong>Lab Notebook</strong>!<br /></h2>
+
    <img src="https://static.igem.org/mediawiki/2015/5/53/Harvard2015_Notebook.png"/>
−
                <p>Will be updated at a later time when we have pieced our sanity back together.</p>
+
                 <h2 class="alt">Our <strong>2015 Lab Notebook</strong>!<br /></h2>
 
               </header>
 
               </header>
  
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<div class="inner-container" >
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    <!-- Footer -->
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<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 6/1</h2>
−
      <div id="footer">
+
<p class="entrytext">First week in lab!
 +
  <br>Microbiology boot camp.
 +
  <br>How to pour plates,
 +
  <br>PCR, miniprep, gel electrophoresis.
 +
  <br>
 +
Researched project ideas.</p></div>
  
−
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+
<br><br>
−
          <li><a href="https://static.igem.org/mediawiki/2015/c/ce/Harvard2015SiteMap.png" target="_blank">Site Map</a></li><li>Site template designed by: <a href="http://html5up.net">HTML5 UP</a></li>
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−
          </ul>
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<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 6/8</h2>
 +
<p class="entrytext">Settle on bacterial adhesion.
 +
<br>
 +
Further background research.
 +
<br>
 +
Plasmid design, primer design.
 +
<br>
 +
Chose fusion peptides.
 +
<br>
 +
Ordered gBlocks from IDT of Rhamnose, Lac, and Arabinose promoters.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 6/15</h2>
 +
<p class="entrytext">PCR backbones and fim proteins.
 +
  <br>
 +
Poured Chloramphenicol and Ampicillin plates.
 +
<br>
 +
Gibson assembly of operon plasmid and fimH plasmid.
 +
<br>
 +
Pili purification assay development.
 +
<br>
 +
NEGEM Meetup #1.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 6/22</h2>
 +
<p class="entrytext">Redo failed PCRs.
 +
  <br>
 +
Redo failed Gibsons.
 +
<br>
 +
Transform and plate operon Gibson.
 +
<br>
 +
Pili purification and agglutination assay development.
 +
<br>
 +
Microscopy on control strains.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 6/29</h2>
 +
<p class="entrytext">Miniprep operon plasmid.
 +
  <br>
 +
Sent for sequencing.
 +
<br>
 +
Unsuccessful- redo operon PCRs.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 7/6</h2>
 +
<p class="entrytext">Induction.
 +
  <br>
 +
Operon Gibson.
 +
<br>
 +
Agglutination assay development.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 7/13</h2>
 +
<p class="entrytext">Induction.
 +
  <br>
 +
Edit out illegal PstI cut site from fimH.
 +
<br>
 +
Mutagenesis to add binding peptides.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 7/20</h2>
 +
<p class="entrytext">Induction.
 +
  <br>
 +
Transform measurement study parts.
 +
<br>
 +
Mutagenesis to add binding peptides.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 7/27</h2>
 +
<p class="entrytext">Induction.
 +
  <br>
 +
Nickel binding assay.
 +
<br>
 +
Made biobricks for pRha-fimH and fimH. PCR-ing out from parts we were using with low-copy backbone, Gibson with pSB1C3.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 8/2</h2>
 +
<p class="entrytext">Induction. Induction. More Induction.
 +
  <br>
 +
Nickel binding assay.
 +
<br>
 +
Stainless steel binding assay.
 +
<br>
 +
Cancer binding assay.
 +
<br>
 +
Made biobricks, sent for sequencing.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
 
 +
<div class="entry"><img style="width:100%;" src="https://static.igem.org/mediawiki/2015/e/e7/Harvard_Notebook_2.jpg" /></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week 8/9</h2>
 +
<p class="entrytext">Induction. Induction. More Induction.
 +
Nickel binding assay.
 +
Stainless steel binding assay.
 +
Cancer binding assay.
 +
Retried failed biobricks.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 8/16</h2>
 +
<p class="entrytext">Break from lab.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 8/23</h2>
 +
<p class="entrytext">Break from lab.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 8/30</h2>
 +
<p class="entrytext">Wiki and presentation planning.
 +
</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 9/6</h2>
 +
<p class="entrytext">Retried failed biobricks.
 +
 
 +
Obtained results for Rhamnose-GFP inductions.
 +
 
 +
Made presentation.
 +
 
 +
NEGEM Meetup #2!</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">Week of 9/13</h2>
 +
<p class="entrytext">Worked on parts pages.
 +
Worked on wiki.</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img src="https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png"/><h2 class="day">9/24 - 9/28</h2>
 +
<p class="entrytext">iGEM Giant Jamboree!!!</p></div>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div class="entry"><img style="width:100%;" src="https://static.igem.org/mediawiki/2015/7/79/Harvard_Notebook_3.jpg" /></div>
 +
</div>
 +
</div>
 +
</div> <!--Intro-->
 +
</section>
 +
 
 +
<section id="notes" class="two">
 +
<div class="container">
 +
 
 +
</div></section>
 +
 
 +
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 +
  <div id="footer">
 +
 
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 +
      <li>Site template designed by: <a href="http://html5up.net">HTML5 UP</a></li>
 +
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 +
 
 +
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Latest revision as of 01:54, 19 September 2015

Prologue by HTML5 UP

Our 2015 Lab Notebook!

Week of 6/1

First week in lab!
Microbiology boot camp.
How to pour plates,
PCR, miniprep, gel electrophoresis.
Researched project ideas.



Week of 6/8

Settle on bacterial adhesion.
Further background research.
Plasmid design, primer design.
Chose fusion peptides.
Ordered gBlocks from IDT of Rhamnose, Lac, and Arabinose promoters.



Week of 6/15

PCR backbones and fim proteins.
Poured Chloramphenicol and Ampicillin plates.
Gibson assembly of operon plasmid and fimH plasmid.
Pili purification assay development.
NEGEM Meetup #1.



Week of 6/22

Redo failed PCRs.
Redo failed Gibsons.
Transform and plate operon Gibson.
Pili purification and agglutination assay development.
Microscopy on control strains.



Week of 6/29

Miniprep operon plasmid.
Sent for sequencing.
Unsuccessful- redo operon PCRs.



Week of 7/6

Induction.
Operon Gibson.
Agglutination assay development.



Week of 7/13

Induction.
Edit out illegal PstI cut site from fimH.
Mutagenesis to add binding peptides.



Week of 7/20

Induction.
Transform measurement study parts.
Mutagenesis to add binding peptides.



Week of 7/27

Induction.
Nickel binding assay.
Made biobricks for pRha-fimH and fimH. PCR-ing out from parts we were using with low-copy backbone, Gibson with pSB1C3.



Week of 8/2

Induction. Induction. More Induction.
Nickel binding assay.
Stainless steel binding assay.
Cancer binding assay.
Made biobricks, sent for sequencing.





Week 8/9

Induction. Induction. More Induction. Nickel binding assay. Stainless steel binding assay. Cancer binding assay. Retried failed biobricks.



Week of 8/16

Break from lab.



Week of 8/23

Break from lab.



Week of 8/30

Wiki and presentation planning.



Week of 9/6

Retried failed biobricks. Obtained results for Rhamnose-GFP inductions. Made presentation. NEGEM Meetup #2!



Week of 9/13

Worked on parts pages. Worked on wiki.



9/24 - 9/28

iGEM Giant Jamboree!!!