Difference between revisions of "Team:Sherbrooke/Experiments"
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− | + | <h1>Hardware Experiments & Protocols</h1> | |
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− | <h1>Experiments & Protocols</h1> | + | |
<h2>Projects modules</h2> | <h2>Projects modules</h2> | ||
<hr> | <hr> | ||
<ul> | <ul> | ||
− | |||
<li><a href="#MC1.5">MC1.5</a></li> | <li><a href="#MC1.5">MC1.5</a></li> | ||
<li><a href="#TAC">TAC</a></li> | <li><a href="#TAC">TAC</a></li> | ||
+ | <li><a href="#MC96">MC96</a></li> | ||
</ul> | </ul> | ||
− | <span id=" | + | <span id="MC1.5"> </span> |
− | <h2> | + | <h2>MC1.5</h2> |
<hr> | <hr> | ||
− | |||
<p> | <p> | ||
− | + | <a href="#MC1.5 Thermal Experimentations">Thermal</a> and <a href="#MC1.5 Magnetisation Experimentations">magnetisation</a> experimentations have been conduct to validate | |
+ | the design of the <i>MC1.5</i> module. | ||
</p> | </p> | ||
− | </ | + | |
+ | <span id="MC1.5 Thermal Experimentations"> </span> | ||
+ | <h3>Thermal experimentation</h3> | ||
+ | <hr> | ||
<h4>Simulation</h4> | <h4>Simulation</h4> | ||
<p> | <p> | ||
− | Thermal simulations have been done on the software COMSOL. These simulations have been used | + | Thermal simulations have been done on the software COMSOL. |
− | + | These simulations have been used to verify the heat transfer | |
− | design. For the | + | of the aluminium mold of the modules, thus helping us improving |
− | + | their design. For the MC1.5, many simulations have been done on different designs. | |
+ | These are the simulation parameters for the latest design. | ||
</p> | </p> | ||
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<h5>Simulation parameters</h5> | <h5>Simulation parameters</h5> | ||
<table> | <table> | ||
Line 176: | Line 40: | ||
<tr> | <tr> | ||
<td>Peltier element cooling power</td> | <td>Peltier element cooling power</td> | ||
− | <td> | + | <td>30W</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Peltier element heating power</td> | <td>Peltier element heating power</td> | ||
− | <td> | + | <td>140W</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
Line 203: | Line 67: | ||
</tr> | </tr> | ||
</table> | </table> | ||
+ | <a href="https://2015.igem.org/Team:Sherbrooke/Results#MC1.5%20Thermal%20Experimentations%20Results">See Results</a> | ||
+ | </br> | ||
<a href="#MC1.5">Back to MC1.5</a> | <a href="#MC1.5">Back to MC1.5</a> | ||
Line 208: | Line 74: | ||
<h4>Trials protocols</h4> | <h4>Trials protocols</h4> | ||
<p> | <p> | ||
− | These are the protocol used to test the thermal | + | These are the protocol used to test the thermal characteristics of the <i>MC1.5</i> prototype. |
These protocols have been tested on a single sub-module of a <i>MC1.5</i> . | These protocols have been tested on a single sub-module of a <i>MC1.5</i> . | ||
</p> | </p> | ||
− | <span id="MC1.5 Protocols"> </span> | + | <div class="imageContainer"> |
− | <h4> | + | <img width="50%" height="50%" src="https://static.igem.org/mediawiki/2015/e/ed/Sherbrooke_MC1.5_Thermal_Setup.jpg" /><br/> |
+ | <p>MC1.5 Thermal Experimentations Setup</p> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <span id="MC1.5 Thermal Experimentations Protocols"> </span> | ||
+ | <h4>Thermal Experimentations Protocols</h4> | ||
<ul> | <ul> | ||
<li><a href="#MC1.5_Maintain_Cold">Maintaining a temperature below room temperature test</a></li> | <li><a href="#MC1.5_Maintain_Cold">Maintaining a temperature below room temperature test</a></li> | ||
Line 225: | Line 97: | ||
<span id="MC1.5_Maintain_Cold"> </span> | <span id="MC1.5_Maintain_Cold"> </span> | ||
<h5>Maintaining a temperature below room temperature test</h5> | <h5>Maintaining a temperature below room temperature test</h5> | ||
− | < | + | <font color="#565656">Purpose</font> |
<p> | <p> | ||
Determine if the module temperature stability fits the specified of ±1.5℃, when the | Determine if the module temperature stability fits the specified of ±1.5℃, when the | ||
Line 231: | Line 103: | ||
the set temperature relation. | the set temperature relation. | ||
</p> | </p> | ||
− | < | + | <font color="#565656">Material</font> |
<ul> | <ul> | ||
<li><i>MC1.5</i> sub-module</li> | <li><i>MC1.5</i> sub-module</li> | ||
Line 239: | Line 111: | ||
</ul> | </ul> | ||
− | < | + | <font color="#565656">Setup</font> |
<ol> | <ol> | ||
<li>Connect the power supply (Topward 6303D) to the fan</li> | <li>Connect the power supply (Topward 6303D) to the fan</li> | ||
Line 246: | Line 118: | ||
to the Peltier element (PS vcc to Peltier gnd and PS gnd to Peltier vcc)</li> | to the Peltier element (PS vcc to Peltier gnd and PS gnd to Peltier vcc)</li> | ||
<li>Set the thermocouple probe at the bottom of the middle hole of the aluminium mold</li> | <li>Set the thermocouple probe at the bottom of the middle hole of the aluminium mold</li> | ||
− | <li>Wait for the thermometer measure to stabilize for 20 | + | <li>Wait for the thermometer measure to stabilize for 20 seconds</li> |
</ol> | </ol> | ||
− | < | + | <font color="#565656">Measurement</font> |
<ol> | <ol> | ||
<li>Set the voltage of the high current power supply to 1V</li> | <li>Set the voltage of the high current power supply to 1V</li> | ||
− | <li>Wait for thermometer measure to stabilize for at least 20 | + | <li>Wait for thermometer measure to stabilize for at least 20 seconds</li> |
<li>Note the thermometer measure and the voltage associated with it</li> | <li>Note the thermometer measure and the voltage associated with it</li> | ||
<li>Repeats set 1, 2 and 3 and increased the voltage by 1V each time until the thermometer measure is below the specified lower limit (0℃)</li> | <li>Repeats set 1, 2 and 3 and increased the voltage by 1V each time until the thermometer measure is below the specified lower limit (0℃)</li> | ||
Line 258: | Line 130: | ||
<li>Stop the fan power supply</li> | <li>Stop the fan power supply</li> | ||
</ol> | </ol> | ||
− | <a href="#MC1.5 Protocols">Back to MC1.5 Protocols</a> | + | <br> |
+ | <a href="https://2015.igem.org/Team:Sherbrooke/Results#MC1.5_Maintain_Cold_results">See Results</a> | ||
+ | </br> | ||
+ | <a href="#MC1.5 Thermal Experimentations Protocols">Back to MC1.5 Thermal Experimentations Protocols</a> | ||
<span id="MC1.5_Maintain_Hot"> </span> | <span id="MC1.5_Maintain_Hot"> </span> | ||
<h5>Maintaining a temperature over room temperature test</h5> | <h5>Maintaining a temperature over room temperature test</h5> | ||
− | < | + | <font color="#565656">Purpose</font> |
<p> | <p> | ||
Determine if the module temperature stability fits the specification of ±1.5℃, when the | Determine if the module temperature stability fits the specification of ±1.5℃, when the | ||
Line 269: | Line 144: | ||
the set temperature relation. | the set temperature relation. | ||
</p> | </p> | ||
− | < | + | <font color="#565656">Material</font> |
<ul> | <ul> | ||
<li><i>MC1.5</i> sub-module</li> | <li><i>MC1.5</i> sub-module</li> | ||
Line 277: | Line 152: | ||
</ul> | </ul> | ||
− | < | + | <font color="#565656">Setup</font> |
<ol> | <ol> | ||
<li>Connect the power supply (Topward 6303D) to the fan</li> | <li>Connect the power supply (Topward 6303D) to the fan</li> | ||
Line 284: | Line 159: | ||
to the Peltier element (PS vcc to Peltier vcc and PS gnd to Peltier gnd)</li> | to the Peltier element (PS vcc to Peltier vcc and PS gnd to Peltier gnd)</li> | ||
<li>Set the thermocouple probe at the bottom of the middle hole of the aluminium mold</li> | <li>Set the thermocouple probe at the bottom of the middle hole of the aluminium mold</li> | ||
− | <li>Wait for the thermometer measure to stabilize for 20 | + | <li>Wait for the thermometer measure to stabilize for 20 seconds</li> |
</ol> | </ol> | ||
− | < | + | <font color="#565656">Measurement</font> |
<ol> | <ol> | ||
<li>Set the voltage of the high current power supply to 1V</li> | <li>Set the voltage of the high current power supply to 1V</li> | ||
− | <li>Wait for thermometer measure to stabilize for at least 20 | + | <li>Wait for thermometer measure to stabilize for at least 20 seconds</li> |
<li>Note the thermometer measure and the voltage associated with it</li> | <li>Note the thermometer measure and the voltage associated with it</li> | ||
<li>Repeats set 1, 2 and 3 and increased the voltage by 1V each time until the thermometer measure is over the specified upper limit (80℃)</li> | <li>Repeats set 1, 2 and 3 and increased the voltage by 1V each time until the thermometer measure is over the specified upper limit (80℃)</li> | ||
Line 296: | Line 171: | ||
<li>Stop the fan power supply</li> | <li>Stop the fan power supply</li> | ||
</ol> | </ol> | ||
− | <a href="#MC1.5 Protocols">Back to MC1.5 Protocols</a> | + | <br> |
+ | <a href="https://2015.igem.org/Team:Sherbrooke/Results#MC1.5_Maintain_Hot_results">See Results</a> | ||
+ | </br> | ||
+ | <a href="#MC1.5 Thermal Experimentations Protocols">Back to MC1.5 Thermal Experimentations Protocols</a> | ||
<span id="MC1.5_to_Cold"> </span> | <span id="MC1.5_to_Cold"> </span> | ||
<h5>Cooling speed test</h5> | <h5>Cooling speed test</h5> | ||
− | < | + | <font color="#565656">Purpose</font> |
<p> | <p> | ||
Determine if the module cooling speed fits the specification of 0.5 to 1℃/s. | Determine if the module cooling speed fits the specification of 0.5 to 1℃/s. | ||
Also, this test determines the optimal voltage to apply to cool the aluminium mold. | Also, this test determines the optimal voltage to apply to cool the aluminium mold. | ||
</p> | </p> | ||
− | < | + | <font color="#565656">Material</font> |
<ul> | <ul> | ||
<li><i>MC1.5</i> sub-module</li> | <li><i>MC1.5</i> sub-module</li> | ||
Line 315: | Line 193: | ||
</ul> | </ul> | ||
− | < | + | <font color="#565656">Setup</font> |
<ol> | <ol> | ||
<li>Connect the power supply (Topward 6303D) to the fan</li> | <li>Connect the power supply (Topward 6303D) to the fan</li> | ||
Line 324: | Line 202: | ||
</ol> | </ol> | ||
− | < | + | <font color="#565656">Measurement</font> |
<ol> | <ol> | ||
<li>Set the voltage of the high current power supply to reach 85℃</li> | <li>Set the voltage of the high current power supply to reach 85℃</li> | ||
− | <li>Wait for thermometer measure to stabilize for at least 20 | + | <li>Wait for thermometer measure to stabilize for at least 20 seconds</li> |
<li>Stop the high current power supply </li> | <li>Stop the high current power supply </li> | ||
<li>Invert connection between the Peltier element and the high current power supply</li> | <li>Invert connection between the Peltier element and the high current power supply</li> | ||
<li>Set the high current power supply to 15.5V (calculated by this <a href="#MethodCoolingVoltage">method</a>)</li> | <li>Set the high current power supply to 15.5V (calculated by this <a href="#MethodCoolingVoltage">method</a>)</li> | ||
<li>Start the chronometer when the thermometer measure reach 80℃</li> | <li>Start the chronometer when the thermometer measure reach 80℃</li> | ||
− | <li>For each 10℃ temperature drop, note the timestamp until | + | <li>For each 10℃ temperature drop, note the timestamp until 4℃ is reached</li> |
<li>Stop the high current power supply </li> | <li>Stop the high current power supply </li> | ||
<li>Invert connection between the Peltier element and the high current power supply</li> | <li>Invert connection between the Peltier element and the high current power supply</li> | ||
<li>Repeats step 1 to 9 for cooling voltage of 15V and 16V </li> | <li>Repeats step 1 to 9 for cooling voltage of 15V and 16V </li> | ||
</ol> | </ol> | ||
− | <a href="#MC1. | + | <br> |
− | + | <a href="https://2015.igem.org/Team:Sherbrooke/Results#MC1.5_to_Cold_results">See Results</a> | |
− | + | </br> | |
<span id="MethodCoolingVoltage"> </span> | <span id="MethodCoolingVoltage"> </span> | ||
− | < | + | </br> |
− | <a href="#MC1.5 Protocols">Back to MC1.5 Protocols</a> | + | <font color="#565656">Theoretical method to determine the optimised cooling voltage</font> |
+ | <p> | ||
+ | This method is an iterative method that is used to approximate the voltage | ||
+ | to apply to the Peltier element to cool the aluminium mold. | ||
+ | </p> | ||
+ | <p> | ||
+ | Logically the more power applied to the Peltier element | ||
+ | the more power is removes from the aluminium mold, thus | ||
+ | increasing its cooling speed. However, the power to disperse | ||
+ | by the heat sink is too high, so the hot side of the Peltier element | ||
+ | is so hot that the temperature difference between the hot side and the | ||
+ | cool side is not enough to reach 0℃. | ||
+ | </p> | ||
+ | <p> | ||
+ | The following figure, from the <a href="http://tetech.com/wp-content/uploads/2013/11/VT-199-1.4-1.15.pdf">Peltier element datasheet</a>, | ||
+ | shows the relation between the power to dissipate by the heat sink | ||
+ | versus the temperature difference between the hot side and cold side (ΔT). | ||
+ | </p> | ||
+ | <div class="imageContainer"> | ||
+ | <img width="50%" height="50%" src="https://static.igem.org/mediawiki/2015/9/9a/Sherbrooke_Peltier_element_wasted_heat_vs_deltaT.png" /><br/> | ||
+ | <p>Peltier element Waste Heat vs ΔT</p> | ||
+ | </div> | ||
+ | <p> | ||
+ | On the following graph, a ΔT is set to 60℃ and the voltage to 24.4V, | ||
+ | thus giving 190W to dissipate. | ||
+ | </p> | ||
+ | <p> | ||
+ | The following equation gives the hot side temperature giving these parameters: | ||
+ | </p> | ||
+ | <div align="center"> | ||
+ | t<sub>h</sub> = t<sub>amb</sub> + Q<sub>h</sub> * R<sub>heat sink</sub> | ||
+ | </div> | ||
+ | <p> | ||
+ | t<sub>h</sub> = Hot side temperature (℃)</br> | ||
+ | t<sub>amb</sub> = Ambient temperature (℃)</br> | ||
+ | Q<sub>h</sub> = Power to dissipate (W)</br> | ||
+ | R<sub>heat sink</sub> = Thermal resistance of the heat sink (℃/W) | ||
+ | </p> | ||
+ | <p> | ||
+ | The heat sink thermal resistance have been tested and characterized | ||
+ | at 0.22℃/W and the ambient temperature to 22℃, thus, giving a hot | ||
+ | side temperature of 63.8℃. By subtracting the set ΔT to this result, | ||
+ | a temperature of 3.8℃ is obtained on the cool side. This is over the | ||
+ | specification of 0℃. | ||
+ | </p> | ||
+ | <p> | ||
+ | So, another iteration of the method with a lower voltage and ΔT is necessary. | ||
+ | </p> | ||
+ | <p> | ||
+ | After a couple of iterations, the voltage of 15.5V and the ΔT of 40℃ have given | ||
+ | the specification of 0℃. | ||
+ | </p> | ||
+ | |||
+ | </br> | ||
+ | <a href="#MC1.5 Thermal Experimentations Protocols">Back to MC1.5 Thermal Experimentations Protocols</a> | ||
<span id="MC1.5_to_Hot"> </span> | <span id="MC1.5_to_Hot"> </span> | ||
<h5>Heating speed test</h5> | <h5>Heating speed test</h5> | ||
− | < | + | <font color="#565656">Purpose</font> |
<p> | <p> | ||
Determine if the module heating speed fits the specified 0.5 to 1℃/s. | Determine if the module heating speed fits the specified 0.5 to 1℃/s. | ||
</p> | </p> | ||
− | < | + | <font color="#565656">Material</font> |
<ul> | <ul> | ||
<li><i>MC1.5</i> sub-module</li> | <li><i>MC1.5</i> sub-module</li> | ||
Line 359: | Line 291: | ||
</ul> | </ul> | ||
− | < | + | <font color="#565656">Setup</font> |
<ol> | <ol> | ||
<li>Connect the power supply (Topward 6303D) to the fan</li> | <li>Connect the power supply (Topward 6303D) to the fan</li> | ||
Line 368: | Line 300: | ||
</ol> | </ol> | ||
− | < | + | <font color="#565656">Measurement</font> |
<ol> | <ol> | ||
− | <li>Set the voltage of the high current power supply to reach - | + | <li>Set the voltage of the high current power supply to reach -1℃</li> |
<li>Wait for thermometer measure to stabilize for at least 20 second</li> | <li>Wait for thermometer measure to stabilize for at least 20 second</li> | ||
<li>Stop the high current power supply </li> | <li>Stop the high current power supply </li> | ||
<li>Invert connection between the Peltier element and the high current power supply</li> | <li>Invert connection between the Peltier element and the high current power supply</li> | ||
<li>Set the high current power supply to 24V (Maximal voltage available for the Peltier element)</li> | <li>Set the high current power supply to 24V (Maximal voltage available for the Peltier element)</li> | ||
− | <li>Start the chronometer when the thermometer measure reach | + | <li>Start the chronometer when the thermometer measure reach 4℃</li> |
<li>For each 10℃ temperature rise, note the timestamp until 80℃ is reached</li> | <li>For each 10℃ temperature rise, note the timestamp until 80℃ is reached</li> | ||
<li>Stop the high current power supply </li> | <li>Stop the high current power supply </li> | ||
</ol> | </ol> | ||
− | <a href="#MC1.5 Protocols">Back to MC1.5 Protocols</a> | + | <br> |
+ | <a href="https://2015.igem.org/Team:Sherbrooke/Results#MC1.5_to_Hot_results">See Results</a> | ||
+ | </br> | ||
+ | <a href="#MC1.5 Thermal Experimentations Protocols">Back to MC1.5 Thermal Experimentations Protocols</a> | ||
+ | </br> | ||
+ | <a href="#MC1.5 Thermal Experimentations">Back to Thermal Experimentations</a> | ||
</br> | </br> | ||
<a href="#MC1.5">Back to MC1.5</a> | <a href="#MC1.5">Back to MC1.5</a> | ||
</br> | </br> | ||
<a href="#top_menu_under">Back to top</a> | <a href="#top_menu_under">Back to top</a> | ||
+ | |||
+ | </br> | ||
+ | <span id="MC1.5 Magnetisation Experimentations"> </span> | ||
+ | <h3>Magnetisation experimentations</h3> | ||
+ | <hr> | ||
+ | <h4>Trials Protocols</h4> | ||
+ | <p> | ||
+ | These are the protocols used to test the magnetisation characteristics | ||
+ | of the MC1.5 prototype. These protocols have been tested on a single | ||
+ | sub-module of a MC1.5. | ||
+ | </p> | ||
+ | <p> | ||
+ | Applying an electromagnetic field on the test tube liquid is one of the key functionality | ||
+ | of the MC1.5. This experiment was conduct in order to confirm that the neodymium magnets | ||
+ | are powerful enough. | ||
+ | </p> | ||
+ | |||
+ | <h5>Magnet attraction power test</h5> | ||
+ | <font color="#565656">Purpose</font> | ||
+ | <p> | ||
+ | Determine if the neodymium magnets are powerful enough to attract the | ||
+ | microscopic magnetic beads on the side of the test tube within 5 minutes. | ||
+ | </p> | ||
+ | <font color="#565656">Material</font> | ||
+ | <ul> | ||
+ | <li>1.5 ml test tube with liquid and magnetic beads inside</li> | ||
+ | <li><a href="http://www.kjmagnetics.com/proddetail.asp?prod=D36">Neodymium magnet (Type N42)</a></li> | ||
+ | <li>Metric Ruler</li> | ||
+ | <li>Chronometer</li> | ||
+ | </ul> | ||
+ | |||
+ | <font color="#565656">Setup</font> | ||
+ | <ol> | ||
+ | <li>Agitate the 1.5ml test tube to ensure that the magnetic beads are spreads through the liquid</li> | ||
+ | <li>Place the 1.5ml test tube at the end of the ruler</li> | ||
+ | <li>Place the center of the magnet in the same relative position as in the MC1.5 module | ||
+ | (5mm from the bottom of the test tube and 4mm from the side of the test tube)</li> | ||
+ | </ol> | ||
+ | |||
+ | <font color="#565656">Measurement</font> | ||
+ | <ol> | ||
+ | <li>As soon as the magnet is in position, start the chronometer</li> | ||
+ | <li>Stop the chronometer when the liquid has the same transparency as distilled water</li> | ||
+ | <li>Note the timestamp on the chronometer</li> | ||
+ | </ol> | ||
+ | <br> | ||
+ | <a href="https://2015.igem.org/Team:Sherbrooke/Results#MC1.5%20Magnet%20attraction%20test%20results">See Results</a> | ||
+ | </br> | ||
+ | <a href="#MC1.5 Magnetisation Experimentations">Back to MC1.5 Magnetisation Experimentations</a> | ||
+ | </br> | ||
+ | <a href="#MC1.5">Back to MC1.5</a> | ||
+ | </br> | ||
+ | <a href="#top_menu_under">Back to top</a> | ||
+ | |||
<span id="TAC"> </span> | <span id="TAC"> </span> | ||
<h2>TAC</h2> | <h2>TAC</h2> | ||
<hr> | <hr> | ||
+ | <p> | ||
+ | <a href="#TAC Thermal Experimentations">Thermal</a> and <a href="#TAC Turbidity Experimentations">turbidity</a> experimentations have been conducted to validate | ||
+ | the design of the <i>TAC</i> module. | ||
+ | </p> | ||
+ | |||
+ | <span id="TAC Thermal Experimentations"> </span> | ||
+ | <h3>Thermal experimentation</h3> | ||
+ | <hr> | ||
+ | <h4>Simulation</h4> | ||
+ | <p> | ||
+ | Thermal simulations have been done on the software COMSOL. | ||
+ | These simulations have been used to verify the heat transfer | ||
+ | of the aluminium mold of the modules, thus helping us improving | ||
+ | their design. For the TAC, many simulations have been done with | ||
+ | different designs. These are the parameters of the simulation for the latest design. | ||
+ | </p> | ||
+ | <h5>Simulation parameters</h5> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <th>Parameters</th> | ||
+ | <th>Values</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Peltier element cooling power</td> | ||
+ | <td>30W</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Peltier element heating power</td> | ||
+ | <td>140W</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Air convective heat transfer coefficient</td> | ||
+ | <td>50W/(m<sup>2</sup> ℃)</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Isolation conductive heat transfer coefficient</td> | ||
+ | <td>5W/(m ℃)</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Aluminium type</td> | ||
+ | <td>6061-t6</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Aluminium conductive heat transfer coefficient</td> | ||
+ | <td>167W/(m ℃)</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Aluminium specific heat capacity</td> | ||
+ | <td>0.896J/(g ℃)</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <a href="https://2015.igem.org/Team:Sherbrooke/Results#TAC%20Thermal%20Experimentations%20Results">See Results</a> | ||
+ | </br> | ||
+ | <a href="#TAC">Back to TAC</a> | ||
+ | |||
+ | <h4>Trials protocols</h4> | ||
+ | <p> | ||
+ | These are the protocol used to test the thermal characteristic of the <i>TAC</i> prototype. | ||
+ | These protocols have been tested on a single sub-module of a <i>TAC</i> . | ||
+ | </p> | ||
+ | <div class="imageContainer"> | ||
+ | <img width="50%" height="50%" src="https://static.igem.org/mediawiki/2015/4/48/Sherbrooke_TAC_Thermal_setup.jpeg" /><br/> | ||
+ | <p>TAC Thermal Experimentations Setup</p> | ||
+ | </div> | ||
+ | |||
+ | <span id="TAC Thermal Experimentations Protocols"> </span> | ||
+ | <h4>Thermal Experimentations Protocols</h4> | ||
+ | <ul> | ||
+ | <li><a href="#TAC_Maintain_Cold">Maintaining a temperature below room temperature test</a></li> | ||
+ | <li><a href="#TAC_Maintain_Hot">Maintaining a temperature over room temperature test</a></li> | ||
+ | <li><a href="#TAC_to_Cold">Cooling speed test</a></li> | ||
+ | <li><a href="#TAC_to_Hot">Heating speed test</a></li> | ||
+ | </ul> | ||
+ | |||
+ | |||
+ | <span id="TAC_Maintain_Cold"> </span> | ||
+ | <h5>Maintaining a temperature below room temperature test</h5> | ||
+ | <font color="#565656">Purpose</font> | ||
+ | <p> | ||
+ | Determine if the module temperature stability fits the specified of ±1.5℃, when the | ||
+ | set temperature is below room temperature. Also, this test determines the voltage versus | ||
+ | the set temperature relation. | ||
+ | </p> | ||
+ | <font color="#565656">Material</font> | ||
+ | <ul> | ||
+ | <li><i>TAC</i> sub-module</li> | ||
+ | <li>High current power supply (<a href="http://www.bkprecision.com/products/power-supplies/1694-1-30v-30a-switching-dc-power-supply-with-remote-sense.html">bk precision 1694 power supply</a>) </li> | ||
+ | <li>Power supply (<a href="http://www.testequity.com/products/1864/">Topward 6303D</a>) </li> | ||
+ | <li>Electronic thermometer (<a href="http://assets.fluke.com/manuals/51______omeng0500.pdf">Fluke 51K/J thermometer</a>) </li> | ||
+ | </ul> | ||
+ | |||
+ | <font color="#565656">Setup</font> | ||
+ | <ol> | ||
+ | <li>Connect the power supply (Topward 6303D) to the fan</li> | ||
+ | <li>Power up the power supply and adjust the voltage to 12V</li> | ||
+ | <li>Connect the high current power supply (bk precision 1694 power supply) | ||
+ | to the Peltier element (PS vcc to Peltier gnd and PS gnd to Peltier vcc)</li> | ||
+ | <li>Set the thermocouple probe at the bottom of the middle hole of the aluminium mold</li> | ||
+ | <li>Wait for the thermometer measure to stabilize for 20 seconds</li> | ||
+ | </ol> | ||
+ | |||
+ | <font color="#565656">Measurement</font> | ||
+ | <ol> | ||
+ | <li>Set the voltage of the high current power supply to 1V</li> | ||
+ | <li>Wait for thermometer measure to stabilize for at least 20 seconds</li> | ||
+ | <li>Note the thermometer measure and the voltage associated with it</li> | ||
+ | <li>Repeats set 1, 2 and 3 and increased the voltage by 1V each time until the thermometer measure is below the specified lower limit (0℃)</li> | ||
+ | <li>Stop the high current power supply</li> | ||
+ | <li>Stop the fan power supply</li> | ||
+ | </ol> | ||
+ | <br> | ||
+ | <a href="https://2015.igem.org/Team:Sherbrooke/Results#TAC_Maintain_Cold_results">See Results</a> | ||
+ | </br> | ||
+ | <a href="#TAC Thermal Experimentations Protocols">Back to TAC Thermal Experimentations Protocols</a> | ||
+ | |||
+ | |||
+ | <span id="TAC_Maintain_Hot"> </span> | ||
+ | <h5>Maintaining a temperature over room temperature test</h5> | ||
+ | <font color="#565656">Purpose</font> | ||
+ | <p> | ||
+ | Determine if the module temperature stability fits the specification of ±1.5℃, when the | ||
+ | set temperature is over room temperature. Also, this test determines the voltage versus | ||
+ | the set temperature relation. | ||
+ | </p> | ||
+ | <font color="#565656">Material</font> | ||
+ | <ul> | ||
+ | <li><i>TAC</i> sub-module</li> | ||
+ | <li>High current power supply (<a href="http://www.bkprecision.com/products/power-supplies/1694-1-30v-30a-switching-dc-power-supply-with-remote-sense.html">bk precision 1694 power supply</a>) </li> | ||
+ | <li>Power supply (<a href="http://www.testequity.com/products/1864/">Topward 6303D</a>) </li> | ||
+ | <li>Electronic thermometer (<a href="http://assets.fluke.com/manuals/51______omeng0500.pdf">Fluke 51K/J thermometer</a>) </li> | ||
+ | </ul> | ||
+ | |||
+ | <font color="#565656">Setup</font> | ||
+ | <ol> | ||
+ | <li>Connect the power supply (Topward 6303D) to the fan</li> | ||
+ | <li>Power up the power supply and adjust the voltage to 12V</li> | ||
+ | <li>Connect the high current power supply (bk precision 1694 power supply) | ||
+ | to the Peltier element (PS vcc to Peltier vcc and PS gnd to Peltier gnd)</li> | ||
+ | <li>Set the thermocouple probe at the bottom of the middle hole of the aluminium mold</li> | ||
+ | <li>Wait for the thermometer measure to stabilize for 20 seconds</li> | ||
+ | </ol> | ||
+ | |||
+ | <font color="#565656">Measurement</font> | ||
+ | <ol> | ||
+ | <li>Set the voltage of the high current power supply to 1V</li> | ||
+ | <li>Wait for thermometer measure to stabilize for at least 20 seconds</li> | ||
+ | <li>Note the thermometer measure and the voltage associated with it</li> | ||
+ | <li>Repeats set 1, 2 and 3 and increased the voltage by 1V each time until the thermometer measure is over the specified upper limit (37℃)</li> | ||
+ | <li>Stop the high current power supply</li> | ||
+ | <li>Stop the fan power supply</li> | ||
+ | </ol> | ||
+ | <br> | ||
+ | <a href="https://2015.igem.org/Team:Sherbrooke/Results#TAC_Maintain_Hot_results">See Results</a> | ||
+ | </br> | ||
+ | <a href="#TAC Thermal Experimentations Protocols">Back to TAC Thermal Experimentations Protocols</a> | ||
+ | |||
+ | |||
+ | <span id="TAC_to_Cold"> </span> | ||
+ | <h5>Cooling speed test</h5> | ||
+ | <font color="#565656">Purpose</font> | ||
+ | <p> | ||
+ | Determine if the module cooling speed fit the specification of 0.3℃/s above room temperature | ||
+ | and 0.2℃/s under room temperature. Also, this test determines the optimal voltage to apply | ||
+ | to cool the aluminium mold. | ||
+ | </p> | ||
+ | <font color="#565656">Material</font> | ||
+ | <ul> | ||
+ | <li><i>TAC</i> sub-module</li> | ||
+ | <li>High current power supply (<a href="http://www.bkprecision.com/products/power-supplies/1694-1-30v-30a-switching-dc-power-supply-with-remote-sense.html">bk precision 1694 power supply</a>) </li> | ||
+ | <li>Power supply (<a href="http://www.testequity.com/products/1864/">Topward 6303D</a>) </li> | ||
+ | <li>Electronic thermometer (<a href="http://assets.fluke.com/manuals/51______omeng0500.pdf">Fluke 51K/J thermometer</a>) </li> | ||
+ | <li>Chronometer</li> | ||
+ | </ul> | ||
+ | |||
+ | <font color="#565656">Setup</font> | ||
+ | <ol> | ||
+ | <li>Connect the power supply (Topward 6303D) to the fan</li> | ||
+ | <li>Power up the power supply and adjust the voltage to 12V</li> | ||
+ | <li>Connect the high current power supply (bk precision 1694 power supply) | ||
+ | to the Peltier element (PS vcc to Peltier vcc and PS gnd to Peltier gnd)</li> | ||
+ | <li>Set the thermocouple probe at the bottom of the middle hole of the aluminium mold</li> | ||
+ | </ol> | ||
+ | |||
+ | <font color="#565656">Measurement</font> | ||
+ | <ol> | ||
+ | <li>Set the voltage of the high current power supply to reach 42℃</li> | ||
+ | <li>Wait for thermometer measure to stabilize for at least 20 seconds</li> | ||
+ | <li>Stop the high current power supply </li> | ||
+ | <li>Invert connection between the Peltier element and the high current power supply</li> | ||
+ | <li>Set the high current power supply to 15.5V (calculated by this <a href="#MethodCoolingVoltage">method</a>)</li> | ||
+ | <li>Start the chronometer when the thermometer measure reach 37℃</li> | ||
+ | <li>For each 2℃ temperature drop, note the timestamp until 0℃ is reached</li> | ||
+ | <li>Stop the high current power supply </li> | ||
+ | <li>Invert connection between the Peltier element and the high current power supply</li> | ||
+ | <li>Repeats step 1 to 9 for cooling voltage of 15V and 16V </li> | ||
+ | </ol> | ||
+ | <br> | ||
+ | <a href="https://2015.igem.org/Team:Sherbrooke/Results#TAC_to_Cold_results">See Results</a> | ||
+ | </br> | ||
+ | <a href="#TAC Thermal Experimentations Protocols">Back to TAC Thermal Experimentations Protocols</a> | ||
+ | |||
+ | |||
+ | <span id="TAC_to_Hot"> </span> | ||
+ | <h5>Heating speed test</h5> | ||
+ | <font color="#565656">Purpose</font> | ||
+ | <p> | ||
+ | Determine if the module heating speed fits the specified 0.5 to 1℃/s. | ||
+ | </p> | ||
+ | <font color="#565656">Material</font> | ||
+ | <ul> | ||
+ | <li><i>TAC</i> sub-module</li> | ||
+ | <li>High current power supply (<a href="http://www.bkprecision.com/products/power-supplies/1694-1-30v-30a-switching-dc-power-supply-with-remote-sense.html">bk precision 1694 power supply</a>) </li> | ||
+ | <li>Power supply (<a href="http://www.testequity.com/products/1864/">Topward 6303D</a>) </li> | ||
+ | <li>Electronic thermometer (<a href="http://assets.fluke.com/manuals/51______omeng0500.pdf">Fluke 51K/J thermometer</a>) </li> | ||
+ | <li>Chronometer</li> | ||
+ | </ul> | ||
+ | |||
+ | <font color="#565656">Setup</font> | ||
+ | <ol> | ||
+ | <li>Connect the power supply (Topward 6303D) to the fan</li> | ||
+ | <li>Power up the power supply and adjust the voltage to 12V</li> | ||
+ | <li>Connect the high current power supply (bk precision 1694 power supply) | ||
+ | to the Peltier element (PS vcc to Peltier gnd and PS gnd to Peltier vcc)</li> | ||
+ | <li>Set the thermocouple probe at the bottom of the middle hole of the aluminium mold</li> | ||
+ | </ol> | ||
+ | |||
+ | <font color="#565656">Measurement</font> | ||
+ | <ol> | ||
+ | <li>Set the voltage of the high current power supply to reach -5℃</li> | ||
+ | <li>Wait for thermometer measure to stabilize for at least 20 seconds</li> | ||
+ | <li>Stop the high current power supply </li> | ||
+ | <li>Invert connection between the Peltier element and the high current power supply</li> | ||
+ | <li>Set the high current power supply to 24V (Maximal voltage available for the Peltier element)</li> | ||
+ | <li>Start the chronometer when the thermometer measure reach 0℃</li> | ||
+ | <li>For each 5℃ temperature rise, note the timestamp until 37℃ is reached</li> | ||
+ | <li>Stop the high current power supply </li> | ||
+ | </ol> | ||
+ | <br> | ||
+ | <a href="https://2015.igem.org/Team:Sherbrooke/Results#TAC_to_Hot_results">See Results</a> | ||
+ | </br> | ||
+ | <a href="#TAC Thermal Experimentations Protocols">Back to TAC Thermal Experimentations Protocols</a> | ||
+ | </br> | ||
+ | <a href="#TAC Thermal Experimentations">Back to TAC Thermal Experimentations</a> | ||
+ | </br> | ||
+ | <a href="#TAC">Back to TAC</a> | ||
+ | </br> | ||
+ | <a href="#top_menu_under">Back to top</a> | ||
+ | </br> | ||
+ | |||
+ | <span id="TAC Turbidity Experimentations"> </span> | ||
+ | <h3>Turbidity experimentations</h3> | ||
+ | <hr> | ||
+ | <p> | ||
+ | One of the main features of the TAC is the ability to measure the optical density | ||
+ | of the liquid inside the test tube. This measure could be used to calculate the | ||
+ | population of microorganism in the liquid. This experiment was conducted to calibrate | ||
+ | the optical density measurement. | ||
+ | </p> | ||
+ | |||
+ | <h5>Protocol</h5> | ||
+ | <font color="#565656">Purpose</font> | ||
+ | <p> | ||
+ | Calibrate the optical density measurement in the TAC. | ||
+ | </p> | ||
+ | <font color="#565656">Material</font> | ||
+ | <ul> | ||
+ | <li><i>TAC</i> sub-module</li> | ||
+ | <li>Reference test tube filled with liquid with different known optical density</li> | ||
+ | </ul> | ||
+ | |||
+ | <font color="#565656">Setup</font> | ||
+ | <ol> | ||
+ | <li>Power up the TAC module</li> | ||
+ | <li>Start the turbidity function (only amplitude difference is shown on screen)</li> | ||
+ | </ol> | ||
+ | |||
+ | <font color="#565656">Measurement</font> | ||
+ | <ol> | ||
+ | <li>Place a reference test tube in the TAC's aluminium mold</li> | ||
+ | <li>Note the amplitude difference output</li> | ||
+ | <li>Repeat step 1 and 2 with a different test tube</li> | ||
+ | </ol> | ||
+ | <br> | ||
+ | <a href="https://2015.igem.org/Team:Sherbrooke/Results#TAC%20Turbidity%20Experimentations%20Results">See Results</a> | ||
+ | </br> | ||
+ | <a href="#TAC">Back to TAC</a> | ||
+ | </br> | ||
+ | <a href="#top_menu_under">Back to top</a> | ||
+ | <hr> | ||
+ | |||
+ | <span id="MC96"> </span> | ||
+ | <h2>MC96</h2> | ||
+ | <hr> | ||
+ | <p> | ||
+ | A <a href="#MC96 Thermal Experimentations">thermal experimentation </a>has been the only experimentation done on the <i>MC96</i> module. | ||
+ | </p> | ||
+ | <span id="MC96 Thermal Experimentations"> </span> | ||
+ | <h3>Thermal experimentations</h3> | ||
+ | <hr> | ||
+ | <p> | ||
+ | The only experimentations done are simulations because no prototype has been built yet. | ||
+ | </p> | ||
+ | <h4>Simulation</h4> | ||
+ | <p> | ||
+ | Thermal simulations have been done on the software COMSOL. These simulations have been used | ||
+ | to verify the heat transfer of the aluminium mold of the modules, thus helping us improve their | ||
+ | design. For the <i>MC96</i>, some simulation has been done on early design, but none on the final design, | ||
+ | due to the complexity of simulating heat pipes. | ||
+ | </p> | ||
+ | |||
+ | <h5>Simulation parameters</h5> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <th>Parameters</th> | ||
+ | <th>Values</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Peltier element cooling power</td> | ||
+ | <td>4X30W</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Peltier element heating power</td> | ||
+ | <td>4X140W</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Air convective heat transfer coefficient</td> | ||
+ | <td>50W/(m<sup>2</sup> ℃)</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Isolation conductive heat transfer coefficient</td> | ||
+ | <td>5W/(m ℃)</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Aluminium type</td> | ||
+ | <td>6061-t6</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Aluminium conductive heat transfer coefficient</td> | ||
+ | <td>167W/(m ℃)</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Aluminium specific heat capacity</td> | ||
+ | <td>0.896J/(g ℃)</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <a href="https://2015.igem.org/Team:Sherbrooke/Results#MC96%20Thermal%20Experimentations%20Results">See Results</a> | ||
+ | </br> | ||
+ | <a href="#MC96">Back to MC96</a> | ||
+ | </br> | ||
+ | <a href="#top_menu_under">Back to top</a> | ||
+ | |||
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Latest revision as of 01:58, 19 September 2015
Hardware Experiments & Protocols
Projects modules
MC1.5
Thermal and magnetisation experimentations have been conduct to validate the design of the MC1.5 module.
Thermal experimentation
Simulation
Thermal simulations have been done on the software COMSOL. These simulations have been used to verify the heat transfer of the aluminium mold of the modules, thus helping us improving their design. For the MC1.5, many simulations have been done on different designs. These are the simulation parameters for the latest design.
Simulation parameters
Parameters | Values |
---|---|
Peltier element cooling power | 30W |
Peltier element heating power | 140W |
Air convective heat transfer coefficient | 50W/(m2 ℃) |
Isolation conductive heat transfer coefficient | 5W/(m ℃) |
Aluminium type | 6061-t6 |
Aluminium conductive heat transfer coefficient | 167W/(m ℃) |
Aluminium specific heat capacity | 0.896J/(g ℃) |
Trials protocols
These are the protocol used to test the thermal characteristics of the MC1.5 prototype. These protocols have been tested on a single sub-module of a MC1.5 .
![](https://static.igem.org/mediawiki/2015/e/ed/Sherbrooke_MC1.5_Thermal_Setup.jpg)
MC1.5 Thermal Experimentations Setup
Thermal Experimentations Protocols
- Maintaining a temperature below room temperature test
- Maintaining a temperature over room temperature test
- Cooling speed test
- Heating speed test
Maintaining a temperature below room temperature test
PurposeDetermine if the module temperature stability fits the specified of ±1.5℃, when the set temperature is below room temperature. Also, this test determines the voltage versus the set temperature relation.
Material- MC1.5 sub-module
- High current power supply (bk precision 1694 power supply)
- Power supply (Topward 6303D)
- Electronic thermometer (Fluke 51K/J thermometer)
- Connect the power supply (Topward 6303D) to the fan
- Power up the power supply and adjust the voltage to 12V
- Connect the high current power supply (bk precision 1694 power supply) to the Peltier element (PS vcc to Peltier gnd and PS gnd to Peltier vcc)
- Set the thermocouple probe at the bottom of the middle hole of the aluminium mold
- Wait for the thermometer measure to stabilize for 20 seconds
- Set the voltage of the high current power supply to 1V
- Wait for thermometer measure to stabilize for at least 20 seconds
- Note the thermometer measure and the voltage associated with it
- Repeats set 1, 2 and 3 and increased the voltage by 1V each time until the thermometer measure is below the specified lower limit (0℃)
- Stop the high current power supply
- Stop the fan power supply
See Results Back to MC1.5 Thermal Experimentations Protocols
Maintaining a temperature over room temperature test
PurposeDetermine if the module temperature stability fits the specification of ±1.5℃, when the set temperature is over room temperature. Also, this test determines the voltage versus the set temperature relation.
Material- MC1.5 sub-module
- High current power supply (bk precision 1694 power supply)
- Power supply (Topward 6303D)
- Electronic thermometer (Fluke 51K/J thermometer)
- Connect the power supply (Topward 6303D) to the fan
- Power up the power supply and adjust the voltage to 12V
- Connect the high current power supply (bk precision 1694 power supply) to the Peltier element (PS vcc to Peltier vcc and PS gnd to Peltier gnd)
- Set the thermocouple probe at the bottom of the middle hole of the aluminium mold
- Wait for the thermometer measure to stabilize for 20 seconds
- Set the voltage of the high current power supply to 1V
- Wait for thermometer measure to stabilize for at least 20 seconds
- Note the thermometer measure and the voltage associated with it
- Repeats set 1, 2 and 3 and increased the voltage by 1V each time until the thermometer measure is over the specified upper limit (80℃)
- Stop the high current power supply
- Stop the fan power supply
See Results Back to MC1.5 Thermal Experimentations Protocols
Cooling speed test
PurposeDetermine if the module cooling speed fits the specification of 0.5 to 1℃/s. Also, this test determines the optimal voltage to apply to cool the aluminium mold.
Material- MC1.5 sub-module
- High current power supply (bk precision 1694 power supply)
- Power supply (Topward 6303D)
- Electronic thermometer (Fluke 51K/J thermometer)
- Chronometer
- Connect the power supply (Topward 6303D) to the fan
- Power up the power supply and adjust the voltage to 12V
- Connect the high current power supply (bk precision 1694 power supply) to the Peltier element (PS vcc to Peltier vcc and PS gnd to Peltier gnd)
- Set the thermocouple probe at the bottom of the middle hole of the aluminium mold
- Set the voltage of the high current power supply to reach 85℃
- Wait for thermometer measure to stabilize for at least 20 seconds
- Stop the high current power supply
- Invert connection between the Peltier element and the high current power supply
- Set the high current power supply to 15.5V (calculated by this method)
- Start the chronometer when the thermometer measure reach 80℃
- For each 10℃ temperature drop, note the timestamp until 4℃ is reached
- Stop the high current power supply
- Invert connection between the Peltier element and the high current power supply
- Repeats step 1 to 9 for cooling voltage of 15V and 16V
See Results Theoretical method to determine the optimised cooling voltage
This method is an iterative method that is used to approximate the voltage to apply to the Peltier element to cool the aluminium mold.
Logically the more power applied to the Peltier element the more power is removes from the aluminium mold, thus increasing its cooling speed. However, the power to disperse by the heat sink is too high, so the hot side of the Peltier element is so hot that the temperature difference between the hot side and the cool side is not enough to reach 0℃.
The following figure, from the Peltier element datasheet, shows the relation between the power to dissipate by the heat sink versus the temperature difference between the hot side and cold side (ΔT).
![](https://static.igem.org/mediawiki/2015/9/9a/Sherbrooke_Peltier_element_wasted_heat_vs_deltaT.png)
Peltier element Waste Heat vs ΔT
On the following graph, a ΔT is set to 60℃ and the voltage to 24.4V, thus giving 190W to dissipate.
The following equation gives the hot side temperature giving these parameters:
th = Hot side temperature (℃) tamb = Ambient temperature (℃) Qh = Power to dissipate (W) Rheat sink = Thermal resistance of the heat sink (℃/W)
The heat sink thermal resistance have been tested and characterized at 0.22℃/W and the ambient temperature to 22℃, thus, giving a hot side temperature of 63.8℃. By subtracting the set ΔT to this result, a temperature of 3.8℃ is obtained on the cool side. This is over the specification of 0℃.
So, another iteration of the method with a lower voltage and ΔT is necessary.
After a couple of iterations, the voltage of 15.5V and the ΔT of 40℃ have given the specification of 0℃.
Back to MC1.5 Thermal Experimentations ProtocolsHeating speed test
PurposeDetermine if the module heating speed fits the specified 0.5 to 1℃/s.
Material- MC1.5 sub-module
- High current power supply (bk precision 1694 power supply)
- Power supply (Topward 6303D)
- Electronic thermometer (Fluke 51K/J thermometer)
- Chronometer
- Connect the power supply (Topward 6303D) to the fan
- Power up the power supply and adjust the voltage to 12V
- Connect the high current power supply (bk precision 1694 power supply) to the Peltier element (PS vcc to Peltier gnd and PS gnd to Peltier vcc)
- Set the thermocouple probe at the bottom of the middle hole of the aluminium mold
- Set the voltage of the high current power supply to reach -1℃
- Wait for thermometer measure to stabilize for at least 20 second
- Stop the high current power supply
- Invert connection between the Peltier element and the high current power supply
- Set the high current power supply to 24V (Maximal voltage available for the Peltier element)
- Start the chronometer when the thermometer measure reach 4℃
- For each 10℃ temperature rise, note the timestamp until 80℃ is reached
- Stop the high current power supply
See Results Back to MC1.5 Thermal Experimentations Protocols Back to Thermal Experimentations Back to MC1.5 Back to top
Magnetisation experimentations
Trials Protocols
These are the protocols used to test the magnetisation characteristics of the MC1.5 prototype. These protocols have been tested on a single sub-module of a MC1.5.
Applying an electromagnetic field on the test tube liquid is one of the key functionality of the MC1.5. This experiment was conduct in order to confirm that the neodymium magnets are powerful enough.
Magnet attraction power test
PurposeDetermine if the neodymium magnets are powerful enough to attract the microscopic magnetic beads on the side of the test tube within 5 minutes.
Material- 1.5 ml test tube with liquid and magnetic beads inside
- Neodymium magnet (Type N42)
- Metric Ruler
- Chronometer
- Agitate the 1.5ml test tube to ensure that the magnetic beads are spreads through the liquid
- Place the 1.5ml test tube at the end of the ruler
- Place the center of the magnet in the same relative position as in the MC1.5 module (5mm from the bottom of the test tube and 4mm from the side of the test tube)
- As soon as the magnet is in position, start the chronometer
- Stop the chronometer when the liquid has the same transparency as distilled water
- Note the timestamp on the chronometer
See Results Back to MC1.5 Magnetisation Experimentations Back to MC1.5 Back to top
TAC
Thermal and turbidity experimentations have been conducted to validate the design of the TAC module.
Thermal experimentation
Simulation
Thermal simulations have been done on the software COMSOL. These simulations have been used to verify the heat transfer of the aluminium mold of the modules, thus helping us improving their design. For the TAC, many simulations have been done with different designs. These are the parameters of the simulation for the latest design.
Simulation parameters
Parameters | Values |
---|---|
Peltier element cooling power | 30W |
Peltier element heating power | 140W |
Air convective heat transfer coefficient | 50W/(m2 ℃) |
Isolation conductive heat transfer coefficient | 5W/(m ℃) |
Aluminium type | 6061-t6 |
Aluminium conductive heat transfer coefficient | 167W/(m ℃) |
Aluminium specific heat capacity | 0.896J/(g ℃) |
Trials protocols
These are the protocol used to test the thermal characteristic of the TAC prototype. These protocols have been tested on a single sub-module of a TAC .
![](https://static.igem.org/mediawiki/2015/4/48/Sherbrooke_TAC_Thermal_setup.jpeg)
TAC Thermal Experimentations Setup
Thermal Experimentations Protocols
- Maintaining a temperature below room temperature test
- Maintaining a temperature over room temperature test
- Cooling speed test
- Heating speed test
Maintaining a temperature below room temperature test
PurposeDetermine if the module temperature stability fits the specified of ±1.5℃, when the set temperature is below room temperature. Also, this test determines the voltage versus the set temperature relation.
Material- TAC sub-module
- High current power supply (bk precision 1694 power supply)
- Power supply (Topward 6303D)
- Electronic thermometer (Fluke 51K/J thermometer)
- Connect the power supply (Topward 6303D) to the fan
- Power up the power supply and adjust the voltage to 12V
- Connect the high current power supply (bk precision 1694 power supply) to the Peltier element (PS vcc to Peltier gnd and PS gnd to Peltier vcc)
- Set the thermocouple probe at the bottom of the middle hole of the aluminium mold
- Wait for the thermometer measure to stabilize for 20 seconds
- Set the voltage of the high current power supply to 1V
- Wait for thermometer measure to stabilize for at least 20 seconds
- Note the thermometer measure and the voltage associated with it
- Repeats set 1, 2 and 3 and increased the voltage by 1V each time until the thermometer measure is below the specified lower limit (0℃)
- Stop the high current power supply
- Stop the fan power supply
See Results Back to TAC Thermal Experimentations Protocols
Maintaining a temperature over room temperature test
PurposeDetermine if the module temperature stability fits the specification of ±1.5℃, when the set temperature is over room temperature. Also, this test determines the voltage versus the set temperature relation.
Material- TAC sub-module
- High current power supply (bk precision 1694 power supply)
- Power supply (Topward 6303D)
- Electronic thermometer (Fluke 51K/J thermometer)
- Connect the power supply (Topward 6303D) to the fan
- Power up the power supply and adjust the voltage to 12V
- Connect the high current power supply (bk precision 1694 power supply) to the Peltier element (PS vcc to Peltier vcc and PS gnd to Peltier gnd)
- Set the thermocouple probe at the bottom of the middle hole of the aluminium mold
- Wait for the thermometer measure to stabilize for 20 seconds
- Set the voltage of the high current power supply to 1V
- Wait for thermometer measure to stabilize for at least 20 seconds
- Note the thermometer measure and the voltage associated with it
- Repeats set 1, 2 and 3 and increased the voltage by 1V each time until the thermometer measure is over the specified upper limit (37℃)
- Stop the high current power supply
- Stop the fan power supply
See Results Back to TAC Thermal Experimentations Protocols
Cooling speed test
PurposeDetermine if the module cooling speed fit the specification of 0.3℃/s above room temperature and 0.2℃/s under room temperature. Also, this test determines the optimal voltage to apply to cool the aluminium mold.
Material- TAC sub-module
- High current power supply (bk precision 1694 power supply)
- Power supply (Topward 6303D)
- Electronic thermometer (Fluke 51K/J thermometer)
- Chronometer
- Connect the power supply (Topward 6303D) to the fan
- Power up the power supply and adjust the voltage to 12V
- Connect the high current power supply (bk precision 1694 power supply) to the Peltier element (PS vcc to Peltier vcc and PS gnd to Peltier gnd)
- Set the thermocouple probe at the bottom of the middle hole of the aluminium mold
- Set the voltage of the high current power supply to reach 42℃
- Wait for thermometer measure to stabilize for at least 20 seconds
- Stop the high current power supply
- Invert connection between the Peltier element and the high current power supply
- Set the high current power supply to 15.5V (calculated by this method)
- Start the chronometer when the thermometer measure reach 37℃
- For each 2℃ temperature drop, note the timestamp until 0℃ is reached
- Stop the high current power supply
- Invert connection between the Peltier element and the high current power supply
- Repeats step 1 to 9 for cooling voltage of 15V and 16V
See Results Back to TAC Thermal Experimentations Protocols
Heating speed test
PurposeDetermine if the module heating speed fits the specified 0.5 to 1℃/s.
Material- TAC sub-module
- High current power supply (bk precision 1694 power supply)
- Power supply (Topward 6303D)
- Electronic thermometer (Fluke 51K/J thermometer)
- Chronometer
- Connect the power supply (Topward 6303D) to the fan
- Power up the power supply and adjust the voltage to 12V
- Connect the high current power supply (bk precision 1694 power supply) to the Peltier element (PS vcc to Peltier gnd and PS gnd to Peltier vcc)
- Set the thermocouple probe at the bottom of the middle hole of the aluminium mold
- Set the voltage of the high current power supply to reach -5℃
- Wait for thermometer measure to stabilize for at least 20 seconds
- Stop the high current power supply
- Invert connection between the Peltier element and the high current power supply
- Set the high current power supply to 24V (Maximal voltage available for the Peltier element)
- Start the chronometer when the thermometer measure reach 0℃
- For each 5℃ temperature rise, note the timestamp until 37℃ is reached
- Stop the high current power supply
See Results Back to TAC Thermal Experimentations Protocols Back to TAC Thermal Experimentations Back to TAC Back to top
Turbidity experimentations
One of the main features of the TAC is the ability to measure the optical density of the liquid inside the test tube. This measure could be used to calculate the population of microorganism in the liquid. This experiment was conducted to calibrate the optical density measurement.
Protocol
PurposeCalibrate the optical density measurement in the TAC.
Material- TAC sub-module
- Reference test tube filled with liquid with different known optical density
- Power up the TAC module
- Start the turbidity function (only amplitude difference is shown on screen)
- Place a reference test tube in the TAC's aluminium mold
- Note the amplitude difference output
- Repeat step 1 and 2 with a different test tube
See Results Back to TAC Back to top
MC96
A thermal experimentation has been the only experimentation done on the MC96 module.
Thermal experimentations
The only experimentations done are simulations because no prototype has been built yet.
Simulation
Thermal simulations have been done on the software COMSOL. These simulations have been used to verify the heat transfer of the aluminium mold of the modules, thus helping us improve their design. For the MC96, some simulation has been done on early design, but none on the final design, due to the complexity of simulating heat pipes.
Simulation parameters
Parameters | Values |
---|---|
Peltier element cooling power | 4X30W |
Peltier element heating power | 4X140W |
Air convective heat transfer coefficient | 50W/(m2 ℃) |
Isolation conductive heat transfer coefficient | 5W/(m ℃) |
Aluminium type | 6061-t6 |
Aluminium conductive heat transfer coefficient | 167W/(m ℃) |
Aluminium specific heat capacity | 0.896J/(g ℃) |