Difference between revisions of "Team:Tsinghua/Hardware"

• HOME
• TEAM
• PROJECT
  • Description
  • Design
  • Experiments & Results
• PARTS
  • Team Parts
  • Basic Parts
  • Composite Parts
• NOTEBOOK
  • Lablog
  • Protocol
• HARDWARE
• MODELING
• HUMAN PRACTICES
• COLLABORATIONS
• ATTRIBUTIONS
• SAFETY

Hardware: E-light 1.0
The E-light 1.0 hardware system has 3 major components: the light-exposure & bacterial culture system, the controlling circuit and the computer interacting port.
The light-exposure & bacterial culture system is based on a 24-well plate coupled with tri-color LEDs. The controlling circuit utilizes 3 AT89S52-24PU DIP-40 SCMs (single chip microcomputer) to execute programmed-controlling of the 24 tri-color LEDs, while the computer interacting port monitors the whole system through given protocol sequences. The ultimate result is the programmable operation and real-time monitoring of light-exposure (on both timing and wave-length) on every single well.
Table below shows the material and components for this delicate device:

 Software: E-code 1.0

 The  E-code 1.0 software system aims to provide convenient commanding for users of  the E-light hardware system. The software provides two operating modes: the  E.coli-code mode is able to convert any given information into light-coded  files, and therefore turn these files into actual light-exposure commands of  the E-light hardware system. With the help of the coding-plasmids from our  CRISPR-Recombinase system, we can eventually store any information into the  E.coli DNA and of course, extract the information later on through sequencing.  The self-code mode provides more flexible input options, enabling users to  program the light-exposure commands manually for every single  bacterial-culture-unit. Thus, combined with our light-switch, the user is able  to gain better control over the bacteria’s metabolism pathways.

 After successfully constructing all the systems required and  confirming its efficacy, we can bridge the light-switchable TCS and the  dCas9-recombines system together. In this way, precise gene editing and  information storing can be achieved by utilizing the light system to regulate  the dCas9-recombinase hybrid. </p>

________________________________________________________________________________________________________________________