Difference between revisions of "Team:Bielefeld-CeBiTec/Basic Part"

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<h2> Basic Parts</h2>
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<h4>Note</h4>
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<p>In order to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best New Basic Part award</a>, you must fill out this page. Please give links to the Registry entries for the Basic parts you have made. Please see the Registry's <a href="http://parts.igem.org/Help:Parts#Basic_and_Composite_Parts"> Help:Parts page</a> for more information on part types.</p>
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<div class="jumbotron" style="background-image: url(https://static.igem.org/mediawiki/2015/d/d5/Bielefeld-CeBiTec_legosteine_jumbotron_parts.jpeg)">
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<div class="jumbotron-text">
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<h1 style="margin-bottom: 0px">Parts</h1>
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<p>The building blocks of our project.</p>
 
</div>
 
</div>
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</div>
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A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
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    <div class="Subtitle">
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          <h2>Data for CFPS</h2>
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      </div>
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      <p>
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<div class ="element" id="CFPS">
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<table style="background-color:transparent">
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<tr>
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  <th width="30%" style="text-align:left">BioBrick</th><th width="70%" style="text-align:left">Description</th>
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</tr>
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<tr>
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  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758100" target="_blank"> BBa_K1758100</a></td><td> Translation enhancing 5'-untranslated region (UTR) containing g10-L RBS  </td>
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</tr>
 +
    <tr>
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  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758101" target="_blank"> BBa_K1758101</a></td><td> 5'-untranslated region (UTR) containing g10-L RBS in front of sfGFP coding sequence  </td>
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</tr>
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      <tr>
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    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758102" target="_blank"> BBa_K1758102</a></td><td> T7 promoter in front of 5'-untranslated region (UTR) followed by sfGFP coding sequence  </td>
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</tr>
 +
      <tr>
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    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758105" target="_blank"> BBa_K1758105</a></td><td> T7 promoter followed by mRFP generator </td>
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</tr>
 +
    <tr>
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    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758106" target="_blank"> BBa_K1758106</a></td><td> T7 promoter followed by 5'-untranslated region (UTR) and mRFP generator </td>
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</tr>
 +
    <tr>
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    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758107" target="_blank"> BBa_K1758107</a></td><td> T7 promoter and RBS followed by SYFP2 coding sequence </td>
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</tr>
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    <tr>
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    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758120" target="_blank"> BBa_K1758120</a></td><td> Coding sequence of RNase E regulating protein RraA from <i>E. coli</i></td>
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</tr>
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    <tr>
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    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758122" target="_blank"> BBa_K1758122</a></td><td> Coding sequence of RNase E regulating protein RraA under control of T7 promoter, with N-terminal His-tag and TEV cleavage site</td>
 +
</tr>
 +
    </table>
 +
    </div>
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 +
          <h2>Data for PRIA</h2>
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 +
      <p>
 +
<div class ="element" id="PRIA">
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<table style="background-color:transparent">
 +
<tr>
 +
  <th width="30%" style="text-align:left">BioBrick</th><th width="70%" style="text-align:left">Description</th>
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</tr>
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<tr>
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  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758201" target="_blank">BBa_K1758201</a></td><td>Lac Repressor with His-Tag under control of T7 promoter and a strong RBS</td>
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</tr>
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<tr>
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  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758202" target="_blank">BBa_K1758202</a></td><td>Lac repressor fused with superfolding Green Fluorescence Protein (sfGFP) with His-Tag under control of T7 promoter and a strong RBS</td>
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</tr>
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<tr>
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  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758203" target="_blank">BBa_K1758203</a></td>
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  <td>Arsenic repressor fused with superfolding Green Fluorescence Protein (sfGFP) with His-Tag under control of T7 promoter and a strong RBS</td>
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</tr>
 +
  <tr>
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  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758204" target="_blank">BBa_K1758204</a></td>
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  <td>Blc repressor fused with superfolding Green Fluorescence Protein (sfGFP) with His-Tag under control of T7 promoter and a strong RBS</td>
 +
</tr>
 +
</table>
 +
</div>
 
</p>
 
</p>
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<div class="Subtitle">
 +
          <h2>Data for Heavy metals</h2>
 +
      </div>
 +
      <p>
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<div class ="element" id="PRIA">
 +
<table style="background-color:transparent">
 +
<tr>
 +
  <th width="30%" style="text-align:left">BioBrick</th><th width="70%" style="text-align:left">Description</th>
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</tr>
 +
<tr>
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  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758300" target="_blank"> BBa_K1758300</a></td><td>sfGFP controlled by T7 promoter and arsenic operator</td>
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</tr>
 +
<tr>
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  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758301" target="_blank"> BBa_K1758301</a></td><td>His-tagged arsenic repressor (ArsR) under control of the T7 promoter</td>
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</tr>
 +
<tr>
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  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758310" target="_blank"> BBa_K1758310</a></td><td>Chromium Repressor under control of constitutive Promoter and strong RBS </td>
 +
</tr>
 +
<tr>
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  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758311" target="_blank"> BBa_K1758311</a></td><td> Chromium responsive promoter with RFP </td>
 +
</tr>
 +
<tr>
 +
  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758312" target="_blank"> BBa_K1758312</a></td>
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  <td>Chromium responsive promoter with UTR+RBS and sfGFP </td>
 +
</tr>
 +
  <tr>
 +
  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758313" target="_blank"> BBa_K1758313</a></td>
 +
  <td>Chromium repressor under control of constitutive promoter  and strong RBS, chromium responsive promoter, UTR+RBS and sfGFP </td>
 +
</tr>
 +
  <tr>
 +
  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758314" target="_blank"> BBa_K1758314</a></td>
 +
  <td>Chromium responsive promoter with T7-promoter and UTR-sfGFP  </td>
 +
</tr>
 +
  <tr>
 +
  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758320" target="_blank"> BBa_K1758320</a></td>
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  <td>Copper activator under control  constitutive promoter and strong RBS </td>
 +
</tr>
 +
  <tr>
 +
  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758321" target="_blank"> BBa_K1758321</a></td>
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  <td>Copper responsive promoter with RFP </td>
 +
</tr>
 +
  <tr>
 +
  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758322" target="_blank"> BBa_K1758322</a></td>
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  <td> Copper activator under control  constitutive promoter and strong RBS  and Copper responsive promoter with RFP </td>
 +
</tr>
 +
  <tr>
 +
  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758323" target="_blank"> BBa_K1758323</a></td>
 +
  <td>Copper responsive promoter with UTR-sfGFP </td>
 +
</tr>
 +
  <tr>
 +
  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758324" target="_blank"> BBa_K1758324</a></td>
 +
  <td> Copper activator under control  constitutive promoter and strong RBS  and Copper responsive promoter with UTR-sfGFP </td>
 +
</tr>
 +
  <tr>
 +
  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758325" target="_blank"> BBa_K1758325</a></td>
 +
  <td> Copper responsive promoter with T7-promoter and UTR-sfGFP  </td>
 +
</tr>
 +
  <tr>
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  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758330" target="_blank"> BBa_K1758330</a></td>
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  <td>Lead repressor under control of constitutive promot er and strong RBS</td>
 +
</tr>
 +
  <tr>
 +
  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758331" target="_blank"> BBa_K1758331</a></td>
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  <td>Lead responsive promoter with RFP </td>
 +
</tr>
 +
  <tr>
 +
  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758332" target="_blank"> BBa_K1758332</a></td>
 +
  <td>Lead responsive promoter with UTR-sfGFP </td>
 +
</tr>
 +
  <tr>
 +
  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758333" target="_blank"> BBa_K1758333</a></td>
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  <td>Lead repressor under control of constitutive promoter and strong RBS and lead responsive promoter with UTR-sfGFP </td>
 +
</tr>
 +
  <tr>
 +
  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758334" target="_blank"> BBa_K1758334</a></td>
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  <td> Lead responsive promoter with T7-promoter and UTR-sfGFP  </td>
 +
</tr>
 +
  <tr>
 +
  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758340" target="_blank"> BBa_K1758340</a></td>
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  <td>Mercury repressor under control of constitutive promoter and strong RBS </td>
 +
</tr>
 +
  <tr>
 +
  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758342" target="_blank"> BBa_K1758342</a></td>
 +
  <td>Mercury responsive promoter with UTR-sfGFP </td>
 +
</tr>
 +
  <tr>
 +
  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758343" target="_blank"> BBa_K1758343</a></td>
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  <td> Mercury activator under control of constitutive promoter and strong RBS and mercury responsive promoter with UTR-sfGFP </td>
 +
</tr>
 +
  <tr>
 +
  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758344" target="_blank"> BBa_K1758344</a></td>
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  <td> Mercury responsive promoter with T7-promoter and UTR-sfGFP  </td>
 +
</tr>
 +
  <tr>
 +
  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758350" target="_blank"> BBa_K1758350</a></td>
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  <td>Nickel repressor under control of constitutive promoter and strong RBS </td>
 +
</tr>
 +
  <tr>
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  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758351" target="_blank"> BBa_K1758351</a></td>
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  <td>Nickel responsive promoter with RFP </td>
 +
</tr>
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  <tr>
 +
  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758352" target="_blank"> BBa_K1758352</a></td>
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  <td>Nickel responsive promoter with UTR-sfGFP </td>
 +
</tr>
 +
  <tr>
 +
  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758353" target="_blank"> BBa_K1758353</a></td>
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  <td> Nickel repressor under control of constitutive promoter and strong RBS and Nickel responsive promoter with UTR-sfGFP </td>
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</tr>
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  <tr>
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  <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758354" target="_blank"> BBa_K1758354</a></td>
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  <td> Nickel responsive promoter with T7-promoter and UTR-sfGFP </td>
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</tr>
 +
</table>
 +
</div>
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<div class="Subtitle">
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          <h2>Data for Date Rape Drugs</h2>
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      </div>
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      <p>
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<div class ="element" id="daterapedrugs">
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<table style="background-color:transparent">
 +
<tr>
 +
  <th width="30%" style="text-align:left">BioBrick</th><th width="70%" style="text-align:left">Description</th>
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</tr>
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<tr>
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    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758360" target="_blank"> BBa_K1758360</a></td><td> gabR gene and gabT promoter from Bacillus subtilis</td>
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</tr>
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<tr>
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    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758361" target="_blank"> BBa_K1758361</a></td><td> mRFP1 under the control of gabR</td>
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</tr>
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<tr>
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    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758370" target="_blank"> BBa_K1758370</a></td><td> Repressor of <i>blcABC</i> operon, BlcR, under control of constitutive promoter with RBS</td>
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</tr>
 +
    <tr>
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    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758375" target="_blank"> BBa_K1758375</a></td><td> Promoter of <i>blcABC</i> operon followed by mRFP cds and terminator </td>
 +
</tr>
 +
    <tr>
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    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758376" target="_blank"> BBa_K1758376</a></td><td> T7 promoter in front of Blc operator sequence followed by 5'-UTR and sfGFP </td>
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</tr>
 +
    <tr>
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    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1758377" target="_blank"> BBa_K1758377</a></td><td> T7 promoter in front of Blc operator sequence followed by 5'-UTR and sfGFP, combined with repressor BlcR under control of constitutive promoter </td>
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</tr>
 +
    </table>
 +
    </div>
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
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</p>
  
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<div class="Subtitle">
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          <h2>Parts in the Registry</h2>
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      </div>
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      <p>This is the table with all our submitted BioBricks, as provided by the <a href="http://parts.igem.org/Main_Page">Registry of Standard Biological Parts</a></p>
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</html>
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<center><groupparts>iGEM015 Bielefeld-CeBiTec</groupparts></center>
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<div class="row">
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<div class="col-md-2 col-md-offset-10 text-center">
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<a type="button" class="btn btn-default btn-next" href="https://2015.igem.org/Team:Bielefeld-CeBiTec/InterlabStudy"><img src="https://static.igem.org/mediawiki/2015/d/d0/Bielefeld-CeBiTec_teamlogo_button.png"><p>We also participated in this year´s interlab study.</p></a>
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{{Bielefeld-CeBiTec/Footer}}

Latest revision as of 02:14, 19 September 2015

iGEM Bielefeld 2015


Parts

The building blocks of our project.

Data for CFPS

BioBrickDescription
BBa_K1758100 Translation enhancing 5'-untranslated region (UTR) containing g10-L RBS
BBa_K1758101 5'-untranslated region (UTR) containing g10-L RBS in front of sfGFP coding sequence
BBa_K1758102 T7 promoter in front of 5'-untranslated region (UTR) followed by sfGFP coding sequence
BBa_K1758105 T7 promoter followed by mRFP generator
BBa_K1758106 T7 promoter followed by 5'-untranslated region (UTR) and mRFP generator
BBa_K1758107 T7 promoter and RBS followed by SYFP2 coding sequence
BBa_K1758120 Coding sequence of RNase E regulating protein RraA from E. coli
BBa_K1758122 Coding sequence of RNase E regulating protein RraA under control of T7 promoter, with N-terminal His-tag and TEV cleavage site

Data for PRIA

BioBrickDescription
BBa_K1758201Lac Repressor with His-Tag under control of T7 promoter and a strong RBS
BBa_K1758202Lac repressor fused with superfolding Green Fluorescence Protein (sfGFP) with His-Tag under control of T7 promoter and a strong RBS
BBa_K1758203 Arsenic repressor fused with superfolding Green Fluorescence Protein (sfGFP) with His-Tag under control of T7 promoter and a strong RBS
BBa_K1758204 Blc repressor fused with superfolding Green Fluorescence Protein (sfGFP) with His-Tag under control of T7 promoter and a strong RBS

Data for Heavy metals

BioBrickDescription
BBa_K1758300sfGFP controlled by T7 promoter and arsenic operator
BBa_K1758301His-tagged arsenic repressor (ArsR) under control of the T7 promoter
BBa_K1758310Chromium Repressor under control of constitutive Promoter and strong RBS
BBa_K1758311 Chromium responsive promoter with RFP
BBa_K1758312 Chromium responsive promoter with UTR+RBS and sfGFP
BBa_K1758313 Chromium repressor under control of constitutive promoter and strong RBS, chromium responsive promoter, UTR+RBS and sfGFP
BBa_K1758314 Chromium responsive promoter with T7-promoter and UTR-sfGFP
BBa_K1758320 Copper activator under control constitutive promoter and strong RBS
BBa_K1758321 Copper responsive promoter with RFP
BBa_K1758322 Copper activator under control constitutive promoter and strong RBS and Copper responsive promoter with RFP
BBa_K1758323 Copper responsive promoter with UTR-sfGFP
BBa_K1758324 Copper activator under control constitutive promoter and strong RBS and Copper responsive promoter with UTR-sfGFP
BBa_K1758325 Copper responsive promoter with T7-promoter and UTR-sfGFP
BBa_K1758330 Lead repressor under control of constitutive promot er and strong RBS
BBa_K1758331 Lead responsive promoter with RFP
BBa_K1758332 Lead responsive promoter with UTR-sfGFP
BBa_K1758333 Lead repressor under control of constitutive promoter and strong RBS and lead responsive promoter with UTR-sfGFP
BBa_K1758334 Lead responsive promoter with T7-promoter and UTR-sfGFP
BBa_K1758340 Mercury repressor under control of constitutive promoter and strong RBS
BBa_K1758342 Mercury responsive promoter with UTR-sfGFP
BBa_K1758343 Mercury activator under control of constitutive promoter and strong RBS and mercury responsive promoter with UTR-sfGFP
BBa_K1758344 Mercury responsive promoter with T7-promoter and UTR-sfGFP
BBa_K1758350 Nickel repressor under control of constitutive promoter and strong RBS
BBa_K1758351 Nickel responsive promoter with RFP
BBa_K1758352 Nickel responsive promoter with UTR-sfGFP
BBa_K1758353 Nickel repressor under control of constitutive promoter and strong RBS and Nickel responsive promoter with UTR-sfGFP
BBa_K1758354 Nickel responsive promoter with T7-promoter and UTR-sfGFP

Data for Date Rape Drugs

BioBrickDescription
BBa_K1758360 gabR gene and gabT promoter from Bacillus subtilis
BBa_K1758361 mRFP1 under the control of gabR
BBa_K1758370 Repressor of blcABC operon, BlcR, under control of constitutive promoter with RBS
BBa_K1758375 Promoter of blcABC operon followed by mRFP cds and terminator
BBa_K1758376 T7 promoter in front of Blc operator sequence followed by 5'-UTR and sfGFP
BBa_K1758377 T7 promoter in front of Blc operator sequence followed by 5'-UTR and sfGFP, combined with repressor BlcR under control of constitutive promoter

Parts in the Registry

This is the table with all our submitted BioBricks, as provided by the Registry of Standard Biological Parts

<groupparts>iGEM015 Bielefeld-CeBiTec</groupparts>

We also participated in this year´s interlab study.