Difference between revisions of "Team:Jilin China/Description"

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<h2> Project Description </h2>
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<title>describtion</title>
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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<p>Formaldehyde, as  common contamination in daily life, is one of the most dangerous cancerogen in  the world. The condition of formaldehyde pollution has alerted people. Thus, it  is essential for everyone to detect and degrade formaldehyde right now. [1]  However, there is no simple and convenient way to detect and degrade formaldehyde.  On the contrary, available methods are usually too complicated for the public  with a great amount of drawbacks. For example, Spectrophotometry is limited by  experiment conditions and Prices of chromatography equipment are usually high. [1][2][3]  So we want to design biobricks to solve this problem by using microorganism. </p>
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<p><u><strong>How to detect formaldehyde?</strong></u><br>
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  With  corporation of Wuhan University, We got a special promoter named hxlR in Bacillus  subtilis, whose function is that it can be induced by formaldehyde and express  downstream genes.[4] Due to this feature, with the help of GFP, we designed a  vector PSB1C3 by using a series of genes, hxlR and GFP(Figure 1). If this  system works well, we will see fluorescence under UV-light.</p>
  
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<h5>What should this page contain?</h5>
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<img src="https://static.igem.org/mediawiki/2015/2/21/Team_Jilin_China_project1.png" height="150px"> </div>
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<li> A clear and concise description of your project.</li>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<p><big>Figure 1. Vector pSB1C3-hxlR-GFP.</big></p><br>
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<li>References and sources to document your research.</li>
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<h4>Advice on writing your Project Description</h4>
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<p><u>How to degrade formaldehyde?</u></p>
  
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<p> We found two key enzymes to degrade formaldehyde, named formate dehydrogenase (FDH) and formaldehyde dehydrogenase (PADH). Last year, Wuhan University added these two genes downstream Promoter hxlR directly, but did not got perfect results(We also verified, you can click here to see). Therefore, we improved this system.</p>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.  
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<p> First, we adjusted prior vector by adding a gene called T7 RNA polymerase(Figure 2). What’s more, we used pET vectors to express two key enzymes, and constructed such a vector(Figure 3).</p>
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<p> Finally, when T7 polymerase is expressed, Vector pET-DUET can express PADH and FDH. In this way, the whole system can be induced by formaldehyde and express PADH and FDH to degrade it.</p>
  
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<div align="center"> <img src="https://static.igem.org/mediawiki/2015/3/37/Team_Jilin_China_project2.png" height="150px"> </div>
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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<p><big>Figure 2. Vector pSB1C3-hxlR-PADH.</big></p>
  
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<div align="center"> <img src="https://static.igem.org/mediawiki/2015/8/83/Team_Jilin_China_project3.png" height="170px"> </div>
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<p><big>Figure 3. Vector pETDUET-PADH-FDH.</big></p>
  
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<h4>References</h4>
 
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you.</p>
 
  
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<p><strong>Reference</strong>
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<h4>Inspiration</h4>
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<p> 1. ZHANG Wenai. Research progress in analysis methods for formaldehyde. Hongqiao District of Tianjin Centers for Disease Control and Prevention, Tianjin 3001 32, China.</p>
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<p>See how other teams have described and presented their projects: </p>
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<p> 2. WANG Fang-ling，YANG J ian-zhong. Research progress in analysis methods for formaldehyde. Textile and Material College，Xi'an Polytechnic University, Xi'an 7 10048, China.</p>
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<p> 3. ZHANG Xiao-feng, XIANG Jin-xin, FU Yu-jie. Research Progress in Analysis M ethods for Formaldehyde. School of Bioengineering, Chongqing Institute of Technology, Chongqing 400050，China. </p>
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<p>4. Yurimoto H, Hirai R, Matsuno N, et al. HxlR, a member of the DUF24 protein family, is a DNA© binding protein that acts as a positive regulator of the formaldehyde©inducible hxlAB operon in Bacillus subtilis[J]. Molecular microbiology, 2005, 57(2): 511-519.</p>
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<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
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Latest revision as of 02:17, 19 September 2015



describtion

Formaldehyde, as common contamination in daily life, is one of the most dangerous cancerogen in the world. The condition of formaldehyde pollution has alerted people. Thus, it is essential for everyone to detect and degrade formaldehyde right now. [1] However, there is no simple and convenient way to detect and degrade formaldehyde. On the contrary, available methods are usually too complicated for the public with a great amount of drawbacks. For example, Spectrophotometry is limited by experiment conditions and Prices of chromatography equipment are usually high. [1][2][3] So we want to design biobricks to solve this problem by using microorganism.

How to detect formaldehyde?
With corporation of Wuhan University, We got a special promoter named hxlR in Bacillus subtilis, whose function is that it can be induced by formaldehyde and express downstream genes.[4] Due to this feature, with the help of GFP, we designed a vector PSB1C3 by using a series of genes, hxlR and GFP(Figure 1). If this system works well, we will see fluorescence under UV-light.

Figure 1. Vector pSB1C3-hxlR-GFP.


How to degrade formaldehyde?

We found two key enzymes to degrade formaldehyde, named formate dehydrogenase (FDH) and formaldehyde dehydrogenase (PADH). Last year, Wuhan University added these two genes downstream Promoter hxlR directly, but did not got perfect results(We also verified, you can click here to see). Therefore, we improved this system.

First, we adjusted prior vector by adding a gene called T7 RNA polymerase(Figure 2). What’s more, we used pET vectors to express two key enzymes, and constructed such a vector(Figure 3).

Finally, when T7 polymerase is expressed, Vector pET-DUET can express PADH and FDH. In this way, the whole system can be induced by formaldehyde and express PADH and FDH to degrade it.

Figure 2. Vector pSB1C3-hxlR-PADH.

Figure 3. Vector pETDUET-PADH-FDH.

Reference

1. ZHANG Wenai. Research progress in analysis methods for formaldehyde. Hongqiao District of Tianjin Centers for Disease Control and Prevention, Tianjin 3001 32, China.

2. WANG Fang-ling，YANG J ian-zhong. Research progress in analysis methods for formaldehyde. Textile and Material College，Xi'an Polytechnic University, Xi'an 7 10048, China.

3. ZHANG Xiao-feng, XIANG Jin-xin, FU Yu-jie. Research Progress in Analysis M ethods for Formaldehyde. School of Bioengineering, Chongqing Institute of Technology, Chongqing 400050，China.

4. Yurimoto H, Hirai R, Matsuno N, et al. HxlR, a member of the DUF24 protein family, is a DNA© binding protein that acts as a positive regulator of the formaldehyde©inducible hxlAB operon in Bacillus subtilis[J]. Molecular microbiology, 2005, 57(2): 511-519.