Difference between revisions of "Team:USTC/Software"

 
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  <div class="col offset-m1 offset-l2 s12 m10 l8">
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    <div class="col offset-m1 offset-l2 s12 m10 l8">
  
    <div id="Overview" class="row">
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        <div id="Overview" class="row">
      <div class="card hoverable">
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            <div class="card hoverable">
        <div class="col s12 m9">
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                <div class="col s12 m9">
          <div class="card-content">
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                    <div class="card-content">
<p>In this section, we will introduce all the software program we used when processing our modeling and experimental data. Through our modeling, many complicated processdures are simply omitted by our convenient programs.</p>
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                        <p>In this section, we will introduce all the software program we used when processing our
<p>In general, our software programs are used in several sections,</p>
+
                            modeling and experimental data. Through our modeling, many complicated processdures are
<ul>
+
                            simply omitted by our convenient programs.</p>
    <li><strong>Fringes Analysis</strong>, analysis of interference pattern.</li>
+
 
    <li><strong>Adhesion Dynamics</strong>, a series of programs relating to bacteria adhesion dynamics.</li>
+
                        <p>In general, our software programs are used in several sections,</p>
    <li><strong>Rose Prediction</strong>, which calculate the advantages of our ROSE improvement.</li>
+
                        <ul>
</ul>
+
                            <li><strong>Fringes Analysis</strong>, analysis of interference pattern.</li>
<p>All software of our project, you can download them at <a href="https://github.com/Cintau/2015USTCiGEM" target="_blank">Github:2015USTCiGEM</a>. All codes are based on </p>
+
                            <li><strong>Adhesion Dynamics</strong>, a series of programs relating to bacteria adhesion
          </div>
+
                                dynamics.
 +
                            </li>
 +
                            <li><strong>Rose Prediction</strong>, which calculate the advantages of our ROSE
 +
                                improvement.
 +
                            </li>
 +
                        </ul>
 +
                        <p>All software of our project, you can download them at <a
 +
                                href="https://github.com/Cintau/2015USTCiGEM" target="_blank">Github:2015USTCiGEM</a>.
 +
                            All codes are based on </p>
 +
                    </div>
 +
                </div>
 +
            </div>
 
         </div>
 
         </div>
      </div>
 
    </div>
 
 
    <div id="fringes-analysis" class="row">
 
      <div class="card hoverable">
 
        <div class="col s12 m9">
 
          <div class="card-content">
 
<h4 id="Fringes-Pattern-Simulation-Film-I" class="scrollspy">Fringes Pattern Simulation-Film I</h4>
 
<p>This program is used to simulate fringes pattern deliverd by film I.</p>
 
    <div><textarea id="code" name="code">
 
%numbers
 
[1234 1234i 1234j]
 
[.234 .234j 2.23i]
 
[23e2 12E1j 123D-4 0x234]
 
 
%strings
 
'asda''a'
 
"asda""a"
 
  
%identifiers
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        <div id="fringes-analysis" class="row">
a + as123 - __asd__
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            <div class="card hoverable">
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                <div class="col s12 m9">
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                    <div class="card-content">
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                        <h4 id="Fringes-Pattern-Simulation-Film-I" class="scrollspy">Fringes Pattern Simulation-Film
 +
                            I</h4>
  
%operators
+
                        <p>This program is used to simulate fringes pattern deliverd by film I.</p>
-
+
+
+
=
+
==
+
>
+
<
+
>=
+
<=
+
&
+
~
+
...
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break zeros default margin round ones rand
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ceil floor size clear zeros eye mean std cov
+
error eval function
+
abs acos atan asin cos cosh exp log prod sum
+
log10 max min sign sin sinh sqrt tan reshape
+
return
+
case switch
+
else elseif end if otherwise
+
do for while
+
try catch
+
classdef properties events methods
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global persistent
+
  
%one line comment
+
                        <p>Code:</p>
%{ multi
+
line commment %}
+
  
    </textarea></div>
+
                        <div><textarea id="code" name="code">function drawndh(r,h,t,n) %r is radius of film,h is the distance of deformation,t is the drift angle of film,n is pixel number
<p>Code:</p>
+
<code id="code">function drawndh(r,h,t,n) %r is radius of film,h is the distance of deformation,t is the drift angle of film,n is pixel number
+
 
wl=6.5e-7; %wave length
 
wl=6.5e-7; %wave length
 
R=r.^2/(2*h);
 
R=r.^2/(2*h);
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imshow(I);
 
imshow(I);
 
end
 
end
</code><p>Demo<br><img src="https://static.igem.org/mediawiki/2015/c/cd/0.02-5e-6-5e-4.jpg" alt="Figure 1: Film II deformation simulation result"></p>
+
</textarea></div>
<p>After the formation of demo, we are able to recongize the real situation in our pre-experiment.</p>
+
                        <p>Demo<br><img src="https://static.igem.org/mediawiki/2015/c/cd/0.02-5e-6-5e-4.jpg"
<div class="divider"></div>
+
                                        alt="Figure 1: Film II deformation simulation result"></p>
  
<h4 id="Fringes-Pattern-Simulation-Film-II" class="scrollspy">Fringes Pattern Simulation-Film II</h4>
+
                        <p>After the formation of demo, we are able to recongize the real situation in our
<p>This program is used to simulate fringes pattern deliverd by film II.</p>
+
                            pre-experiment.</p>
<p>Code</p>
+
 
<pre><code id="code">function drawfr(a,b,h,n)%a is the angle between film and x axis, b is the angle between film and y axis, h is the distance of film deformation, n is the pixel number
+
                        <div class="divider"></div>
 +
 
 +
                        <h4 id="Fringes-Pattern-Simulation-Film-II" class="scrollspy">Fringes Pattern Simulation-Film
 +
                            II</h4>
 +
 
 +
                        <p>This program is used to simulate fringes pattern deliverd by film II.</p>
 +
 
 +
                        <p>Code</p>
 +
 
 +
                        <div><textarea id="code1" name="code">function drawfr(a,b,h,n)%a is the angle between film and x axis, b is the angle between film and y axis, h is the distance of film deformation, n is the pixel number
 
[X,Y]=meshgrid(linspace(-0.003,0.003,n),linspace(-0.003,0.003,n));
 
[X,Y]=meshgrid(linspace(-0.003,0.003,n),linspace(-0.003,0.003,n));
 
wl=6.5e-7;
 
wl=6.5e-7;
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imshow(I)
 
imshow(I)
 
end
 
end
</code></pre><p>Demo<br><img src="https://static.igem.org/mediawiki/2015/f/f8/20150917061.jpg" alt="Figure 2 :Film II deformation simulation result"></p>
+
</textarea></div>
<div class="divider"></div>
+
                        <p>Demo<br><img src="https://static.igem.org/mediawiki/2015/f/f8/20150917061.jpg"
 +
                                        alt="Figure 2 :Film II deformation simulation result"></p>
  
<h4 id="Film I Fringes Analysis" class="scrollspy">Film I Fringes Analysis</h4>
+
                        <div class="divider"></div>
<p>Using this code, we are able to capture some important parameters in Film I.</p>
+
 
<p>Code</p>
+
                        <h4 id="Film I Fringes Analysis" class="scrollspy">Film I Fringes Analysis</h4>
<pre><code id="code">function ms3=solvendh(X,nx,ny,n,dx,dy)%nx is the number of fringes on x axis,ny is the number of fringes on y axis,n is the number of fringes on diag,dx is the x length of CCD,dx is the x length of CCD.
+
 
 +
                        <p>Using this code, we are able to capture some important parameters in Film I.</p>
 +
 
 +
                        <p>Code</p>
 +
 
 +
                        <div><textarea id="code2" name="code">function ms3=solvendh(X,nx,ny,n,dx,dy)%nx is the number of fringes on x axis,ny is the number of fringes on y axis,n is the number of fringes on diag,dx is the x length of CCD,dx is the x length of CCD.
 
x=X(1);
 
x=X(1);
 
y=X(2);
 
y=X(2);
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ms3(2)=(2*(z+ny)*l)^0.5-(2*z*l)^0.5-(x^2+(y+dy)^2)^0.5+(x^2+y^2)^0.5;
 
ms3(2)=(2*(z+ny)*l)^0.5-(2*z*l)^0.5-(x^2+(y+dy)^2)^0.5+(x^2+y^2)^0.5;
 
ms3(3)=(2*(z+n)*l)^0.5-(2*z*l)^0.5-((x+dx)^2+(y+dy)^2)^0.5+(x^2+y^2)^0.5;
 
ms3(3)=(2*(z+n)*l)^0.5-(2*z*l)^0.5-((x+dx)^2+(y+dy)^2)^0.5+(x^2+y^2)^0.5;
</code></pre><p>Demo<br><img src="https://static.igem.org/mediawiki/2015/0/0d/20150919071.png" alt="Figure 3 : Film I Fringes analysis running result"></p>
+
</textarea></div>
<div class="divider"></div>
+
                        <p>Demo<br><img src="https://static.igem.org/mediawiki/2015/0/0d/20150919071.png"
 +
                                        alt="Figure 3 : Film I Fringes analysis running result"></p>
 +
 
 +
                        <div class="divider"></div>
 +
 
 +
                        <h4 id="Film-II-Fringes-Analysis-Fringes-number-analysis" class="scrollspy">Film II Fringes
 +
                            Analysis-Fringes number analysis</h4>
 +
 
 +
                        <p>This is the final program used to get our NDM calibration. Based on this software program, we
 +
                            are able to count the number of bright fringes. As a matter of fact, the variation of bright
 +
                            fringes is the key breakthrough to antibiotic analysis.</p>
 +
 
 +
                        <p>Code</p>
  
<h4 id="Film-II-Fringes-Analysis-Fringes-number-analysis" class="scrollspy">Film II Fringes Analysis-Fringes number analysis</h4>
+
                        <div><textarea id="code3" name="code">function seeim(i)
<p>This is the final program used to get our NDM calibration. Based on this software program, we are able to count the number of bright fringes. As a matter of fact, the variation of bright fringes is the key breakthrough to antibiotic analysis.</p>
+
<p>Code</p>
+
<pre><code id="code">function seeim(i)
+
 
name=num2str(i);
 
name=num2str(i);
 
I=imread(name,'pnm');
 
I=imread(name,'pnm');
 
G=rgb2gray(I);
 
G=rgb2gray(I);
 
imshow(G);
 
imshow(G);
</code></pre><p>Demo<br><img src="https://static.igem.org/mediawiki/2015/2/2d/20150919072.png" alt="Figure 4: Checking figure program"></p>
+
</textarea></div>
<pre><code id="code">function A=getfr(i)
+
                        <p>Demo<br><img src="https://static.igem.org/mediawiki/2015/2/2d/20150919072.png"
 +
                                        alt="Figure 4: Checking figure program"></p>
 +
 
 +
                        <div><textarea id="code4" name="code">function A=getfr(i)
 
name=num2str(i);
 
name=num2str(i);
 
I=imread(name,'pnm');
 
I=imread(name,'pnm');
Line 179: Line 178:
 
end
 
end
 
A=r;
 
A=r;
</code></pre><pre><code id="code">function M=getfrs(i)
+
</textarea></div>
 +
                        <div><textarea id="code5" name="code">function M=getfrs(i)
 
A=getfr(i);
 
A=getfr(i);
 
t=1:480;
 
t=1:480;
Line 185: Line 185:
 
[a,k]=size(pksa);
 
[a,k]=size(pksa);
 
M=a;
 
M=a;
</code></pre><p>Running result<br>ans =<br>   78</p>
+
</textarea></div>
<p><img src="https://static.igem.org/mediawiki/2015/c/c1/20150919073.png" alt="Figure 5: Fringes counting program running result"></p>
+
                        <p>Running result<br>ans =<br> 78</p>
<p>To get a series of results, this program is required.</p>
+
 
<pre><code id="code">function N=getpkss(i,j)
+
                        <p><img src="https://static.igem.org/mediawiki/2015/c/c1/20150919073.png"
 +
                                alt="Figure 5: Fringes counting program running result"></p>
 +
 
 +
                        <p>To get a series of results, this program is required.</p>
 +
 
 +
                        <div><textarea id="code6" name="code">function N=getpkss(i,j)
 
B=zeros(j-i+1,1);
 
B=zeros(j-i+1,1);
 
for m=i:j
 
for m=i:j
Line 197: Line 202:
 
end
 
end
 
N=B;
 
N=B;
</code></pre><p>Demo</p>
+
</textarea></div>
<p>ans =</p>
+
                        <p>Demo</p>
<pre><code id="code">78
+
 
 +
                        <p>ans =</p>
 +
 
 +
                        <div><textarea id="code7" name="code">78
 
80
 
80
 
77
 
77
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81
 
81
 
79
 
79
</code></pre>
+
</textarea></div>
  
          </div>
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                    </div>
 +
                </div>
 +
                <div class="col hide-on-small-only m3">
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                    <div class="toc-wrapper pinned">
 +
                        <ul class="section table-of-contents">
 +
                            <li>
 +
                                <a href="#Fringes-Pattern-Simulation-Film-I">Fringes Pattern Simulation-Film I</a>
 +
                            </li>
 +
                            <li>
 +
                                <a href="#Fringes-Pattern-Simulation-Film-II">Fringes Pattern Simulation-Film II</a>
 +
                            </li>
 +
                            <li>
 +
                                <a href="#Film-I-Fringes-Analysis">Film I Fringes Analysis</a>
 +
                            </li>
 +
                            <li>
 +
                                <a href="#Film-II-Fringes-Analysis-Fringes-number-analysis">Film II Fringes
 +
                                    Analysis-Fringes
 +
                                    number analysis</a>
 +
                            </li>
 +
                        </ul>
 +
                    </div>
 +
                </div>
 +
            </div>
 
         </div>
 
         </div>
        <div class="col hide-on-small-only m3">
 
          <div class="toc-wrapper pinned">
 
            <ul class="section table-of-contents">
 
              <li>
 
                <a href="#Fringes-Pattern-Simulation-Film-I">Fringes Pattern Simulation-Film I</a>
 
              </li>
 
              <li>
 
                <a href="#Fringes-Pattern-Simulation-Film-II">Fringes Pattern Simulation-Film II</a>
 
              </li>
 
              <li>
 
                <a href="#Film-I-Fringes-Analysis">Film I Fringes Analysis</a>
 
              </li>
 
              <li>
 
                <a href="#Film-II-Fringes-Analysis-Fringes-number-analysis">Film II Fringes Analysis-Fringes number analysis</a>
 
              </li>
 
            </ul>
 
          </div>
 
        </div>
 
      </div>
 
    </div>
 
  
    <div id="adhesion-dynamics" class="row">
+
        <div id="adhesion-dynamics" class="row">
      <div class="card hoverable">
+
            <div class="card hoverable">
        <div class="col s12 m9">
+
                <div class="col s12 m9">
          <div class="card-content">
+
                    <div class="card-content">
<p>During analysis of ahdesion assay results, we are quite annoyed about counting total bacteria number and moving bacteria number, which is really important for mechanism analysis and results determination. Consequently, this part explains how we efficiently accomplished these tough work based on our software.</p>
+
                        <p>During analysis of ahdesion assay results, we are quite annoyed about counting total bacteria
<h4 id="Bacteria-counting-program" class="scrollspy">Bacteria counting program</h4>
+
                            number and moving bacteria number, which is really important for mechanism analysis and
<p>This program is used to count the total number of bacteria.</p>
+
                            results
<p>Code</p>
+
                            determination. Consequently, this part explains how we efficiently accomplished these tough
<pre><code id="code">function a=shujun(i)
+
                            work
 +
                            based on our software.</p>
 +
                        <h4 id="Bacteria-counting-program" class="scrollspy">Bacteria counting program</h4>
 +
 
 +
                        <p>This program is used to count the total number of bacteria.</p>
 +
 
 +
                        <p>Code</p>
 +
 
 +
                        <div><textarea id="code8" name="code">function a=shujun(i)
 
name=num2str(i);
 
name=num2str(i);
 
I=imread(name,'jpg');
 
I=imread(name,'jpg');
Line 265: Line 283:
 
a=num;
 
a=num;
 
end
 
end
</code></pre><p>Demo</p>
+
</textarea></div>
<p>input image:<br><img src="https://static.igem.org/mediawiki/2015/0/0e/20150919074.jpg" alt="Figure 6: Adhesion Assay image"><br>output result<br>ans =<br>   865</p>
+
                        <p>Demo</p>
<pre><code id="code">function A=mulshu(m)
+
 
 +
                        <p>input image:<br><img src="https://static.igem.org/mediawiki/2015/0/0e/20150919074.jpg"
 +
                                                alt="Figure 6: Adhesion Assay image"><br>output result<br>ans =<br> 865
 +
                        </p>
 +
 
 +
                        <div><textarea id="code13" name="code">function A=mulshu(m)
 
A=zeros(m,1);
 
A=zeros(m,1);
 
for i=1:m
 
for i=1:m
 
     A(i,1)=shujun(i);
 
     A(i,1)=shujun(i);
 
end
 
end
</code></pre>
+
</textarea></div>
<div class="divider"></div>
+
                        <div class="divider"></div>
  
<h4 id="Moving-Bacteria-Counting-Program" class="scrollspy">Moving Bacteria Counting Program</h4>
+
                        <h4 id="Moving-Bacteria-Counting-Program" class="scrollspy">Moving Bacteria Counting
<p>This program is used to count the total number of moving bacteria. Along with bacterial counting program, we finally got the mechanism of polylysine interaction.</p>
+
                            Program</h4>
<p>Code</p>
+
 
<pre><code id="code">function b=act(m,n)
+
                        <p>This program is used to count the total number of moving bacteria. Along with bacterial
 +
                            counting
 +
                            program, we finally got the mechanism of polylysine interaction.</p>
 +
 
 +
                        <p>Code</p>
 +
 
 +
                        <div><textarea id="code9" name="code">function b=act(m,n)
 
s1=num2str(m);
 
s1=num2str(m);
 
s2=num2str(n);
 
s2=num2str(n);
Line 286: Line 315:
 
b=shujun(G);
 
b=shujun(G);
 
end
 
end
</code></pre><pre><code>function A=mulact(m)
+
</textarea></div>
 +
                        <div><textarea id="code14" name="code">function A=mulact(m)
 
A=zeros(m,1);
 
A=zeros(m,1);
 
for i=1:m
 
for i=1:m
Line 296: Line 326:
 
     A(i,1)=c;
 
     A(i,1)=c;
 
end
 
end
</code></pre><p>Demo</p>
+
</textarea></div>
<p>ans =<br>   405 </p>
+
                        <p>Demo</p>
<div class="divider"></div>
+
 
 +
                        <p>ans =<br> 405 </p>
 +
 
 +
                        <div class="divider"></div>
 +
 
 +
                        <h4 id="Bacteria-total-number-time-curve" class="scrollspy">Bacteria total number-time
 +
                            curve</h4>
 +
 
 +
                        <p>Coding</p>
  
<h4 id="Bacteria-total-number-time-curve" class="scrollspy">Bacteria total number-time curve</h4>
+
                        <div><textarea id="code10" name="code">function drawBT1(Ka,Kd,C,Vz,sigma0)%Ka is the adhesive rate of bacteria, Kd is the drop rate of bacteria, C is the density of bacteria solution,Vz is the velocity of bacteria,sigma0 is the maximum density of baacteria on surface
<p>Coding</p>
+
<pre><code>function drawBT1(Ka,Kd,C,Vz,sigma0)%Ka is the adhesive rate of bacteria, Kd is the drop rate of bacteria, C is the density of bacteria solution,Vz is the velocity of bacteria,sigma0 is the maximum density of baacteria on surface
+
 
t=linspace(0,100,101);
 
t=linspace(0,100,101);
 
K=Ka*C*Vz/(Kd*sigma0+Ka*C*Vz);
 
K=Ka*C*Vz/(Kd*sigma0+Ka*C*Vz);
Line 308: Line 344:
 
plot(t,sigma)
 
plot(t,sigma)
 
end
 
end
</code></pre><p>Running result<br><img src="https://static.igem.org/mediawiki/2015/9/99/20150901024.png" alt="Figure 7: Movement number simulation result"></p>
+
</textarea></div>
<div class="divider"></div>
+
                        <p>Running result<br><img src="https://static.igem.org/mediawiki/2015/9/99/20150901024.png"
 +
                                                  alt="Figure 7: Movement number simulation result"></p>
  
<h4 id="Bacteria-movement-number-Time-curve" class="scrollspy">Bacteria movement number-Time curve</h4>
+
                        <div class="divider"></div>
<p>Coding</p>
+
 
<pre><code id="code">function drawBMT(c,b,m0,k)
+
                        <h4 id="Bacteria-movement-number-Time-curve" class="scrollspy">Bacteria movement number-Time
 +
                            curve</h4>
 +
 
 +
                        <p>Coding</p>
 +
 
 +
                        <div><textarea id="code11" name="code">function drawBMT(c,b,m0,k)
 
t=linspace(0,100,101);
 
t=linspace(0,100,101);
 
M=c*(1-exp(-b*t))*m0.*exp(-k*t);
 
M=c*(1-exp(-b*t))*m0.*exp(-k*t);
Line 321: Line 363:
 
title('PAO1-PLL-0 Movement number-time');
 
title('PAO1-PLL-0 Movement number-time');
 
end
 
end
</code></pre><p>Running result<br><img src="https://static.igem.org/mediawiki/2015/8/88/20150907052.png" alt="Figure8:Bacteria movement number-Time simulation result"></p>
+
</textarea></div>
          </div>
+
                        <p>Running result<br><img src="https://static.igem.org/mediawiki/2015/8/88/20150907052.png"
 +
                                                  alt="Figure8:Bacteria movement number-Time simulation result"></p>
 +
                    </div>
 +
                </div>
 +
 
 +
                <div class="col hide-on-small-only m3">
 +
                    <div class="toc-wrapper pinned">
 +
                        <ul class="section table-of-contents">
 +
                            <li>
 +
                                <a href="#Bacteria-counting-program">Bacteria Counting Program</a>
 +
                            </li>
 +
                            <li>
 +
                                <a href="#Moving-Bacteria-Counting-Program">Moving Bacteria Counting Program</a>
 +
                            </li>
 +
                            <li>
 +
                                <a href="#Bacteria-total-number-time-curve">Bacteria Total Number-Time Curve</a>
 +
                            </li>
 +
                            <li>
 +
                                <a href="#Bacteria-movement-number-Time-curve">Bacteria Movement Number-Time Curve</a>
 +
                            </li>
 +
                        </ul>
 +
                    </div>
 +
                </div>
 +
            </div>
 
         </div>
 
         </div>
        <div class="col hide-on-small-only m3">
 
          <div class="toc-wrapper pinned">
 
            <ul class="section table-of-contents">
 
              <li>
 
                <a href="#Bacteria-counting-program">Bacteria Counting Program</a>
 
              </li>
 
              <li>
 
                <a href="#Moving-Bacteria-Counting-Program">Moving Bacteria Counting Program</a>
 
              </li>
 
              <li>
 
                <a href="#Bacteria-total-number-time-curve">Bacteria Total Number-Time Curve</a>
 
              </li>
 
              <li>
 
                <a href="#Bacteria-movement-number-Time-curve">Bacteria Movement Number-Time Curve</a>
 
              </li>
 
            </ul>
 
          </div>
 
        </div>
 
      </div>
 
    </div>
 
  
    <div id="rose-dynamics" class="row">
+
 
      <div class="card hoverable">
+
        <div id="rose-dynamics" class="row">
        <div class="col s12 m9">
+
            <div class="card hoverable">
          <div class="card-content">
+
                <div class="col s12 m9">
<p>This program is used to predict the advantages of ROSE construction. We successfully demonstrate our ROSE will be able to amplify fluorescence siganl.</p>
+
                    <div class="card-content">
<p>Code</p>
+
                        <p>This program is used to predict the advantages of ROSE construction. We successfully
<pre><code id="code">clear all
+
                            demonstrate our
 +
                            ROSE will be able to amplify fluorescence siganl.</p>
 +
 
 +
                        <p>Code</p>
 +
<div><textarea id="code12" name="code">clear all
 
R=R,k=k,k1=k1,k2=k2,k3=k3,k4=k4,k5=k5,k6=k6,k7=k7,k0=k0; %define the constant
 
R=R,k=k,k1=k1,k2=k2,k3=k3,k4=k4,k5=k5,k6=k6,k7=k7,k0=k0; %define the constant
 
t=linspace(0,10); %detecting time range
 
t=linspace(0,10); %detecting time range
Line 370: Line 419:
 
hold on
 
hold on
 
plot(k0*t,t); %print the traditional strategy result in the same image
 
plot(k0*t,t); %print the traditional strategy result in the same image
</code></pre><p>Demo</p>
+
</textarea></div>
<p><img src="https://static.igem.org/mediawiki/2015/1/1b/20150906Circuit.jpg" alt="Figure 9: ROSE Dynamics simulation result" data-image-src="https://static.igem.org/mediawiki/2015/1/1b/20150906Circuit.jpg"></p>
+
                    <p>Demo</p>
          </div>
+
 
 +
                    <p><img src="https://static.igem.org/mediawiki/2015/1/1b/20150906Circuit.jpg"
 +
                            alt="Figure 9: ROSE Dynamics simulation result"
 +
                            data-image-src="https://static.igem.org/mediawiki/2015/1/1b/20150906Circuit.jpg"></p>
 +
                </div>
 +
            </div>
 
         </div>
 
         </div>
      </div>
 
 
     </div>
 
     </div>
  
  </div>
+
    </div>
 
</div>
 
</div>
  
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+
 
<script type="text/javascript" src="https://2015.igem.org/Template:USTC/js/octave?action=raw&amp;ctype=text/javascript"></script>
+
    <script type="text/javascript"
<script>
+
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    <script type="text/javascript"
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            src="https://2015.igem.org/Template:USTC/js/octave?action=raw&amp;ctype=text/javascript"></script>
 +
    <script>
 
       var editor = CodeMirror.fromTextArea(document.getElementById("code"), {
 
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{{USTC/footer}}
 
{{USTC/footer}}

Latest revision as of 02:46, 19 September 2015

In this section, we will introduce all the software program we used when processing our modeling and experimental data. Through our modeling, many complicated processdures are simply omitted by our convenient programs.

In general, our software programs are used in several sections,

  • Fringes Analysis, analysis of interference pattern.
  • Adhesion Dynamics, a series of programs relating to bacteria adhesion dynamics.
  • Rose Prediction, which calculate the advantages of our ROSE improvement.

All software of our project, you can download them at Github:2015USTCiGEM. All codes are based on

Fringes Pattern Simulation-Film I

This program is used to simulate fringes pattern deliverd by film I.

Code:

Demo
Figure 1: Film II deformation simulation result

After the formation of demo, we are able to recongize the real situation in our pre-experiment.

Fringes Pattern Simulation-Film II

This program is used to simulate fringes pattern deliverd by film II.

Code

Demo
Figure 2 :Film II deformation simulation result

Film I Fringes Analysis

Using this code, we are able to capture some important parameters in Film I.

Code

Demo
Figure 3 : Film I Fringes analysis running result

Film II Fringes Analysis-Fringes number analysis

This is the final program used to get our NDM calibration. Based on this software program, we are able to count the number of bright fringes. As a matter of fact, the variation of bright fringes is the key breakthrough to antibiotic analysis.

Code

Demo
Figure 4: Checking figure program

Running result
ans =
78

Figure 5: Fringes counting program running result

To get a series of results, this program is required.

Demo

ans =

During analysis of ahdesion assay results, we are quite annoyed about counting total bacteria number and moving bacteria number, which is really important for mechanism analysis and results determination. Consequently, this part explains how we efficiently accomplished these tough work based on our software.

Bacteria counting program

This program is used to count the total number of bacteria.

Code

Demo

input image:
Figure 6: Adhesion Assay image
output result
ans =
865

Moving Bacteria Counting Program

This program is used to count the total number of moving bacteria. Along with bacterial counting program, we finally got the mechanism of polylysine interaction.

Code

Demo

ans =
405

Bacteria total number-time curve

Coding

Running result
Figure 7: Movement number simulation result

Bacteria movement number-Time curve

Coding

Running result
Figure8:Bacteria movement number-Time simulation result

This program is used to predict the advantages of ROSE construction. We successfully demonstrate our ROSE will be able to amplify fluorescence siganl.

Code

Demo

Figure 9: ROSE Dynamics simulation result

Contact Us

University of Science and Technology of China, No.96, JinZhai Road Baohe District,Hefei,Anhui, 230026,P.R.China.

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