Difference between revisions of "Team:USTC"

 
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<h3>Project Description</h3>
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<h4>Characterization of antibiotics concentration based on chemotaxis and interference(CACCI)</h4>
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<p><b>Better for public health and environment</b></p>
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<p>In China and other developing countries, abusing antibiotics causes severe antibiotic substances contamination and antibiotic resistance issues. According to latest professional evaluation from Chinese Academy of Science(CAS), China used 162,000 tons of antibiotic substances for human(48%) and animal(52%) in 2013, which is tenfold usage of USA and antibiotic substances in high concentration can be detected from many river basins that may potentially harmful to public health and environment.</p>
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<p>Therefore, this year, we USTC developed a novel, original and portable device, which is able to determine trace antibiotics concentration in natural water bodies. We called it CACCI(characterization of trace antibiotics concentration based on chemotaxis and interference).</p>
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<p>We firstly construct an antibiotics sensing circuit with antibiotic sensible promoters, quorum sensing molecules and logic amplification parts to test the efficiency of our design. Genetic modification on bacteria including overexpression of exogenous porin or viroporin and inhibition of efflux system using CRISPR will be implemented to condense antibiotics in bacterial bodies.</p>
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<p>Then we will establish our CACCI based on genetically engineered chemotaxis circuit and amplification part. Bacteria will be designed with a special transmembrane protein with rare amino acid so that bacteria can be put on a polymer membrane that is able to covalently bind to bacteria with alteration. Thus, slight membrane elastic deformation will be accurately detected by interference and we will use Newton Ring to read out the concentration of antibiotics in sample. Finally CACCI will be developed with a traditional dial and a friendly GUI available for personnel without much professional knowledge and complicated training.</p>
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<p>With our CACCI, we hope, antibiotic contamination issue would be treated in specific methods.</p>
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    <li data-menuanchor="welcomePage" class="active"><a href="#welcomePage">Welcome</a></li>
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    <li data-menuanchor="introPage"><a href="#introPage">Brief Intro</a></li>
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    <li data-menuanchor="constitutionPage"><a href="#constitutionPage">Constitutions</a></li>
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    <li data-menuanchor="tutorialPage"><a href="#tutorialPage">Tutorials</a></li>
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    <li data-menuanchor="navigationsPage" data-hover="true" data-activates="navi-dropdown" data-gutter="-16" data-beloworigin="true" class="dropdown-button"><a href="#navigationsPage">Navigations</a></li>
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  <li><a href="https://2015.igem.org/Team:USTC/Description" class="waves-effect waves-light">Project</a></li>
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  <li><a href="https://2015.igem.org/Team:USTC/Modeling" class="waves-effect waves-light">Modeling</a></li>
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  <li><a href="https://2015.igem.org/Team:USTC/Results" class="waves-effect waves-light">Results</a></li>
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  <li><a href="https://2015.igem.org/Team:USTC/Achievements" class="waves-effect waves-light">Achievements</a></li>
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  <li><a href="https://2015.igem.org/Team:USTC/Measurement" class="waves-effect waves-light">Measurement</a></li>
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  <li><a href="https://2015.igem.org/Team:USTC/Software" class="waves-effect waves-light">Software</a></li>
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  <li><a href="https://2015.igem.org/Team:USTC/Parts" class="waves-effect waves-light">Parts</a></li>
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  <li><a href="https://2015.igem.org/Team:USTC/Tutorials" class="waves-effect waves-light">Tutorials</a></li>
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  <li><a href="https://2015.igem.org/Team:USTC/Notebook" class="waves-effect waves-light">Notebook</a></li>
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  <li><a href="https://2015.igem.org/Team:USTC/Safety" class="waves-effect waves-light">Safety</a></li>
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  <li><a href="https://2015.igem.org/Team:USTC/Practices" class="waves-effect waves-light">Policy&Practices</a></li>
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  <li><a href="https://2015.igem.org/Team:USTC/Team" class="waves-effect waves-light">Team</a></li>
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  <li><a href="https://2015.igem.org/Team:USTC/Attributions" class="waves-effect waves-light">Attributions</a></li>
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        <li> <img src="https://static.igem.org/mediawiki/2015/2/22/USTC-index-background-1.jpg"> </li>
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        <li> <img src="https://static.igem.org/mediawiki/2015/6/6e/USTC-index-background-2.jpg"> </li>
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        <h1 class="white-text"><b>Nanomachine Detecting Microbiotics</b></h1> </div>
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        <h2>In the beginning, we extracted antibiotics, assuming it to be Perseus and it was so. Time going, we found antibiotics turned out to be Ares.</h2>
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          <h2 class="white-text">Continue</h2><img src="https://static.igem.org/mediawiki/2015/1/1d/USTC-index-Down.png" style="width: 60px;" /></a>
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          <h5 class="white-text">In natural environment, many antibiotic substances are emitted into river in their active state, which caused tremendously challenges on human health and environment. Thus, this year we USTC develop NDM, a hardware trying to detect those molecules and hold back this situation.  Advantages of NDM including:  </h5>
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          <figure><img src="https://static.igem.org/mediawiki/2015/b/b1/USTC-index-fast.png"></figure>
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          <div>
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              <h5>Rapid</h5>
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              <p>Detection won’t be based on fluorescence signal,  however a brand new way! Then, how much time does a detection assay cost? 1 day? 100 min? Answer is 100 SECONDS!</p>
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          <figure><img src="https://static.igem.org/mediawiki/2015/1/18/USTC-index-toolbox.png"></figure>
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              <h5>Convenient</h5>
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              <p>NDM is portable for people to operate it, and we developed a friendly GUI for better experience. Moreover, we will package bacteria into powder for iGEMers!</p>
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          <figure><img src="https://static.igem.org/mediawiki/2015/c/cd/USTC-index-gear.png"></figure>
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              <h5>Compatible</h5>
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              <p>NDM is compatible for everyone to engineer. We hope genetic modification on device level would expand.  After all, ‘M’ in NDM means much more than microbiotics!</p>
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          <h4 class="white-text">Constitutions of NDM</h4>
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        <div class="col s2"><img src="https://static.igem.org/mediawiki/2015/c/c6/USTC-index-mod-bact.png"></div>
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        <div class="col s2"><img src="https://static.igem.org/mediawiki/2015/1/1d/USTC-index-film.png"></div>
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        <div class="col s2"><br/><img src="https://static.igem.org/mediawiki/2015/c/c4/USTC-index-hako.png" style="z-index: 5;"></div>
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        <div class="col s2"><img src="https://static.igem.org/mediawiki/2015/9/9c/USTC-index-optical-path.png"></div>
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        <div class="col s2"><h5>CACCI</h5><p>Programmed bacteria to detect chemicals whatever you want</p></div>
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        <div class="col s2"><h5>Film</h5><p>Change the movement of bacterial to deformation of film.</p></div>
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        <div class="col s2"><h5>SPRING</h5><p>The main body NDM, platform provided for experiment.</p></div>
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        <div class="col s2"><h5>Optical path</h5><p>Detect the deformation by Michelson Interference.</p></div>
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        <div class="col s2"><h5>GUI</h5><p>Provide a better experience for users.</p></div>
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      </div>
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      <div style="text-align:center;">
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        <a href="#tutorialPage">
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          <img src="https://static.igem.org/mediawiki/2015/1/1d/USTC-index-Down.png" style="width: 60px;" /></a>
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          <h4 class="white-text">Tutorials: Study NDM Right Now!</h4>
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          <figure>
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            <a href="https://2015.igem.org/Team:USTC/Tutorials#Instructional-Video"><img src="https://static.igem.org/mediawiki/2015/3/31/USTC-index-video.png"></a>
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          </figure>
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              <h5>Video</h5>
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              <p>Want to know how to operate NDM? Watch it at here!</p>
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            <a href="https://2015.igem.org/Team:USTC/Tutorials#Instruction-manual"><img src="https://static.igem.org/mediawiki/2015/7/79/USTC-index-ins.png"></a>
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          </figure>
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              <h5>Manual</h5>
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              <p>This manual is a convenient guidebook for you when operating NDM in field! Download it at here!</p>
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            <a href="https://2015.igem.org/Team:USTC/Tutorials#Make-Your-Own"><img src="https://static.igem.org/mediawiki/2015/5/54/USTC-index-DIY.png"></a>
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          </figure>
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              <h5>DIY</h5>
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              <p>Want to make your own NDM? Come and join us at here!</p>
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          <div class="card hoverable">
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            <div class="card-image">
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              <a href="https://2015.igem.org/Team:USTC/Description">
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                <img src="https://static.igem.org/mediawiki/2015/f/f1/USTC-index-Project.png" onclick>
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                <span class="white-text">Project</span></a>
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            </div>
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          </div>
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        </div>
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        <div class="col s3 navi-inview fadeup" id="nav2">
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          <div class="card hoverable">
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            <div class="card-image">
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              <a href="https://2015.igem.org/Team:USTC/Modeling">
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                <img src="https://static.igem.org/mediawiki/2015/b/b4/USTC-index-Modeling.png">
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                <span class="white-text">Modeling</span></a>
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            </div>
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          </div>
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        </div>
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        <div class="col s3 navi-inview fadeup" id="nav3">
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          <div class="card hoverable">
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            <div class="card-image">
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              <a href="https://2015.igem.org/Team:USTC/Results">
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                <img src="https://static.igem.org/mediawiki/2015/4/42/USTC-index-Achievements.png">
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                <span class="white-text">Results</span></a>
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            </div>
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          </div>
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        </div>
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        <div class="col s3 navi-inview fadeup" id="nav4">
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          <div class="card hoverable">
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            <div class="card-image">
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              <a href="https://2015.igem.org/Team:USTC/Tutorials">
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                <img src="https://static.igem.org/mediawiki/2015/d/d5/USTC-index-Tutorials.png">
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                <span class="white-text">Tutorials</span></a>
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            </div>
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          </div>
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        </div>
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      </div>
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      <div class="row">
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          <div class="card hoverable">
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            <div class="card-image">
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              <a href="https://2015.igem.org/Team:USTC/Notebook">
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                <img src="https://static.igem.org/mediawiki/2015/b/b0/USTC-index-Notebook.png">
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                <span class="white-text">Notebook</span></a>
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            </div>
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          </div>
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        </div>
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        <div class="col s3 navi-inview fadeup" id="nav6">
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          <div class="card hoverable">
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            <div class="card-image">
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              <a href="https://2015.igem.org/Team:USTC/Practices">
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                <img src="https://static.igem.org/mediawiki/2015/3/3d/USTC-index-HumanPractice.png">
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                <span class="white-text">Human<br/>Practice</span></a>
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            </div>
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          </div>
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        </div>
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        <div class="col s3 navi-inview fadeup" id="nav7">
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          <div class="card hoverable">
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            <div class="card-image">
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              <a href="https://2015.igem.org/Team:USTC/Collaborations">
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                <img src="https://static.igem.org/mediawiki/2015/8/8b/USTC-index-Collaboration.png">
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                <span class="white-text">Collaborations</span></a>
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            </div>
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          </div>
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        </div>
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        <div class="col s3 navi-inview fadeup" id="nav8">
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          <div class="card hoverable">
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            <div class="card-image">
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              <a href="https://2015.igem.org/Team:USTC/Team">
 +
                <img src="https://static.igem.org/mediawiki/2015/a/a8/USTC-index-Team.png">
 +
                <span class="white-text">Team</span></a>
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            </div>
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          </div>
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        </div>
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      </div>
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    </div>
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    <div class="Footer grey lighten-1">
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      <div class="container">
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        <div class="row">
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          <div class="col s4 offset-s2">
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            <h5 style="margin-bottom: 2px;">Sponsored by <br/><img src="https://static.igem.org/mediawiki/2015/4/4c/Deutsche-bank-logo.png"></h5>
 +
          </div>
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          <div class="col s4">
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            <h5 style="margin-bottom: 0;">Links</h5>
 +
            <p style="margin:0;">
 +
              <a href="mailto:ustc.igem.2015@gmail.com"><img src="https://static.igem.org/mediawiki/2015/9/9d/USTC-Mail.png"></a>
 +
              <a href="http://weibo.com/3929719655/fans?topnav=1&wvr=6&mod=message&need_filter=1"><img src="https://static.igem.org/mediawiki/2015/6/60/USTC-Weibo.png"></a>
 +
              <a href="https://github.com/Cintau/2015USTCiGEM"><img src="https://static.igem.org/mediawiki/2015/3/36/USTC-GitHub.png"></a>
 +
              <a href="https://www.facebook.com/2014ustcchinaigem?ref=bookmarks"><img src="https://static.igem.org/mediawiki/2015/6/6e/USTC-Facebook.png"></a>
 +
            </p>
 +
          </div>
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
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</div>
  
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<h3>Contact us:<br/></h3>
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</html>
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<a href="http://weibo.com/3929719655/fans?topnav=1&wvr=6&mod=message&need_filter=1">Weibo</a><br/>
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<a href="https://www.facebook.com/2014ustcchinaigem?ref=bookmarks">Facebook</a><br/>
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Latest revision as of 02:58, 19 September 2015