Difference between revisions of "Team:EPF Lausanne/Basic Part"

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<h2> Basic Parts</h2>
 
  
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<h4>Note</h4>
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<p>In order to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best New Basic Part award</a>, you must fill out this page. Please give links to the Registry entries for the Basic parts you have made. Please see the Registry's <a href="http://parts.igem.org/Help:Parts#Basic_and_Composite_Parts"> Help:Parts page</a> for more information on part types.</p>
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                <h3>OUR BEST NEW PART</h3>
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A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
 
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
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<img src="https://static.igem.org/mediawiki/2015/1/17/EPF_Lausanne_Parts_J23117Alt.png" style="width:60%">
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<h2>PAM rich URS J23117Alt promoter</h2>
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<a href="http://parts.igem.org/Part:BBa_K1723005"><b>BBa_K1723005</b></a>
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        <font size="4">Bikard et al. used dCas9-ω targeting the promoter PAM rich URS J23117, BBa_K1723001, in order to regulate gene expression [1], using gRNA (single guide RNA). By using our own dCas9-ω system we proved that this promoter can be activated or repressed (see <a href="https://2015.igem.org/Team:EPF_Lausanne/Results">results page</a>). Now, on the model of this promoter, we designed <b>a new, fully synthetic, promoter</b>: PAM rich URS J23117Alt promoter, BBa_K1723005. We mutated the sequence of BBa_K1723001 between and outside the -10 and -35 sequences where the RNA Polymerase binds (see <a href="https://2015.igem.org/Team:EPF_Lausanne/Results">BBa_K1723005 registry page</a> for more details), in order to have <b>another promoter targeted by a different set of sgRNAs</b>. The creation of this part, and its experimental validation (see <a href="https://2015.igem.org/Team:EPF_Lausanne/Results">results page</a>), is very promising for us as it is the proof of the MUTABILITY of the promoters. We can now imagine of designing other new sequences to obtain others promoter/sgRNAs sets creating more and more different transistors-like elements in cells.</font>
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        <h3>References</h3>
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        <p>[1] Bikard, D., Jiang, W., Samai, P., Hochschild, A., Zhang, F., & Marraffini, L. A. (2013). Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic acids research, 41(15), 7429-7437.</p>
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Latest revision as of 03:07, 19 September 2015

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

OUR BEST NEW PART




PAM rich URS J23117Alt promoter






BBa_K1723005

Bikard et al. used dCas9-ω targeting the promoter PAM rich URS J23117, BBa_K1723001, in order to regulate gene expression [1], using gRNA (single guide RNA). By using our own dCas9-ω system we proved that this promoter can be activated or repressed (see results page). Now, on the model of this promoter, we designed a new, fully synthetic, promoter: PAM rich URS J23117Alt promoter, BBa_K1723005. We mutated the sequence of BBa_K1723001 between and outside the -10 and -35 sequences where the RNA Polymerase binds (see BBa_K1723005 registry page for more details), in order to have another promoter targeted by a different set of sgRNAs. The creation of this part, and its experimental validation (see results page), is very promising for us as it is the proof of the MUTABILITY of the promoters. We can now imagine of designing other new sequences to obtain others promoter/sgRNAs sets creating more and more different transistors-like elements in cells.

References

[1] Bikard, D., Jiang, W., Samai, P., Hochschild, A., Zhang, F., & Marraffini, L. A. (2013). Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic acids research, 41(15), 7429-7437.

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits