Difference between revisions of "Team:USTC/Achievements"

 
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        <a class="dropdown-button btn white-text" data-beloworigin="true" data-hover="true" href="#!"
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    <a class="dropdown-button btn white-text" data-beloworigin="true" data-hover="true" href="#!"
          data-activates="Nav-dropdown" style="padding-top:6px;">ACHIEVEMENTS<i
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      data-activates="Nav-dropdown">ACHIEVEMENTS<i class="material-icons">arrow_drop_down</i></a>
                class="material-icons right">arrow_drop_down</i> </a>
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            <li class="tab col l2 m2 s2">
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      <li class="tab col l2 m2 s2">
                <a href="#Permeability_Improvement" class="blue-text active waves-effect waves-light">Permeability Improvement</a>
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        <a href="#achievement" class="blue-text waves-effect waves-light">Achievement</a>
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      </li>
            <li class="tab col l2 m2 s2 ">
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      <li class="tab col l2 m2 s2">
                <a href="#Chemotaxis_Modification" class="blue-text waves-effect waves-light">Chemotaxis Modification</a>
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        <a href="#Medals" class="blue-text waves-effect waves-light">Medals</a>
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      </li>
            <li class="tab col l2 m2 s2">
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      <li class="tab col l2 m2 s2">
                <a href="#Parts" class="blue-text waves-effect waves-light">Parts</a>
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        <a href="#Future-NDM" class="blue-text waves-effect waves-light">Future NDM</a>
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            <li class="tab col l2 m2 s2">
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    </ul>
                <a href="#Medals" class="blue-text waves-effect waves-light">Medals</a>
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        </ul>
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</div>
 
</div>
  
 
<ul id='Nav-dropdown' class='dropdown-content'>
 
<ul id='Nav-dropdown' class='dropdown-content'>
    <li><a href="https://2015.igem.org/Team:USTC" class="waves-effect waves-light">Home</a></li>
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  <li><a href="https://2015.igem.org/Team:USTC" class="waves-effect waves-light">Home</a></li>
    <li><a href="https://2015.igem.org/Team:USTC/Project" class="waves-effect waves-light">Project</a></li>
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  <li><a href="https://2015.igem.org/Team:USTC/Description" class="waves-effect waves-light">Project</a></li>
    <li><a href="https://2015.igem.org/Team:USTC/Modeling" class="waves-effect waves-light">Modeling</a></li>
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  <li><a href="https://2015.igem.org/Team:USTC/Modeling" class="waves-effect waves-light">Modeling</a></li>
    <li><a href="https://2015.igem.org/Team:USTC/Achievements" class="waves-effect waves-light">Achivements</a></li>
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  <li><a href="https://2015.igem.org/Team:USTC/Results" class="waves-effect waves-light">Results</a></li>
    <li><a href="https://2015.igem.org/Team:USTC/Tutorials" class="waves-effect waves-light">Tutorials</a></li>
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  <li><a href="https://2015.igem.org/Team:USTC/Measurement" class="waves-effect waves-light">Measurement</a></li>
    <li><a href="https://2015.igem.org/Team:USTC/Notebook" class="waves-effect waves-light">NoteBook</a></li>
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    <li><a href="https://2015.igem.org/Team:USTC/Humanpractice" class="waves-effect waves-light">Human Practice</a></li>
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    <li><a href="https://2015.igem.org/Team:USTC/Collaboration" class="waves-effect waves-light">Collaboration</a></li>
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  <li><a href="https://2015.igem.org/Team:USTC/Tutorials" class="waves-effect waves-light">Tutorials</a></li>
    <li><a href="https://2015.igem.org/Team:USTC/Attributions" class="waves-effect waves-light">Attributions</a></li>
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  <li><a href="https://2015.igem.org/Team:USTC/Notebook" class="waves-effect waves-light">NoteBook</a></li>
    <li><a href="https://2015.igem.org/Team:USTC/Team" class="waves-effect waves-light">Team</a></li>
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  <li><a href="https://2015.igem.org/Team:USTC/Safety" class="waves-effect waves-light">Safety</a></li>
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  <li><a href="https://2015.igem.org/Team:USTC/Collaborations" class="waves-effect waves-light">Collaborations</a></li>
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  <li><a href="https://2015.igem.org/Team:USTC/Attributions" class="waves-effect waves-light">Attributions</a></li>
 
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    <div class="col offset-m1 offset-l2 s12 m10 l8">
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  <div class="col offset-m1 offset-l2 s12 m10 l8">
  
        <div id="Permeability_Improvement" class="row">
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    <div id="achievement" class="row">
            <div class="card hoverable">
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            <div class="card-content">
                            <p><strong>Extraction of gene fragements</strong><br>We successfully get gene fragements
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            <p>These are what we have accomplished this year:</p>
                                that are used for bacterial permeability improvement, which including, </p>
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            <ul>
                            <ul>
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              <li>We successully constructed our CACCI, genetically modifed bacteria used to sense antibitoics.</li>
                                <li><p>micF and SoxS, these two antibiotic substance responding promoters were extracted
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              <li>We successfully achieved bacteria adhesing to film by treated with polylysine.</li>
                                    from <em>E. coli Top 10(K-12 strain)</em> by conducting polymerase chain reaction on
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              <li>We successfully developed a device called SPRING with optical module and electric circuit.</li>
                                    its genome.<br><img src="https://static.igem.org/mediawiki/2015/2/2b/MicF_and_SoxS.png"
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              <li>We successfully proved adhesion dynamics would be stable after 100s.</li>
                                                        alt="图片名称"></p>
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              <li>We successfully established interference pattern by our SPRING.</li>
                                </li>
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              <li>We successfully proved that interference patterns have difference between each other when treating in different antibiotic concentration.</li>
                                <li><p>SCVE, a viroporin originally from SARS virus, is synthesized from Sangon Co. ctd.<br><img
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              <li>We successully calibrated our NDM for antibiotics determination.</li>
                                        src="https://static.igem.org/mediawiki/2015/1/12/USTC-SCVE.png" alt="图片名称"></p>
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              <li>We successfully developed a series of program used to process our interference pattern and data. </li>
                                </li>
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              <li>We successully demonstrated that our NDM is able to interact with bacteria, and distinguish different antibiotic concentration by delivering different interference pattern.</li>
                                <li>OprF, a bigger porin compared with OmpF in <em>E. coli</em>, is extracted from <em>P.aeruginosa,
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            </ul>
                                    PAO1</em> genome by PCR.<br><img
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                                        src="https://static.igem.org/mediawiki/2015/6/66/USTC-OprF.png" alt="图片名称"></li>
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                                <li>CheZ, a chemotaxis related protein is also extracted from <em>P.aeruginosa,
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                                    PAO1</em> genome by PCR.
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                                </li>
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                                <li>Cas9, from 2015 part registry.<br><img
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                                        src="https://static.igem.org/mediawiki/2015/1/1c/USTC-Cas9.png" alt="图片名称"></li>
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                                <li>gRNA-AcrB and gRNA-EmrE, these two fragments is for genetically modifed bacterial
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                                    transmembrane protein AcrB and EmrE to strongly block drug efflux system.<br><img
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                                            src="https://static.igem.org/mediawiki/2015/5/54/USTC-gRNA_extraction.png"
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                                            alt="图片名称"></li>
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                            </ul>
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                        </div>
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                    </div>
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                </div>
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             </div>
 
             </div>
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          </div>
 
         </div>
 
         </div>
        <div id="Chemotaxis_Modification" class="row">
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      </div>
            <div class="card hoverable">
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                <div class="row">
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    <div id="Medals" class="row">
                        <div class="card-content">
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      <div class="card hoverable">
                            <p><strong>Growth characteristics</strong><br>We detected OD600(nm) to identify the effect
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        <div class="row">
                                of cheZ expression on <em>E. coli</em> through time.<br><img
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          <div class="col s12 m9">
                                        src="https://static.igem.org/mediawiki/2015/d/d6/Ustc-growth.png" alt="图片名称"></p>
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            <div class="card-content">
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              <h4 id="Bronze" class="scrollspy">Bronze:</h4>
  
                            <p>The exogenous protein expression affects the growth of bacteria, which was indicated by
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              <p>Your team must convince the judges you have achieved the following 6 goals:</p>
                                slight slower grow-up in <em>E. coli</em> containing cheZ plasmid. In total, cheZ
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              <ul>
                                doesn't have obvious negative impact on bacteria grown. </p>
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                <li>Register for iGEM, have a great summer, and attend the Giant Jamboree.</li>
 +
                <li>Complete the Judging form.</li>
 +
                <li>Create and share a Description of the team's project using the iGEM wiki, and
 +
                  document the team's parts (if any) using the Registry of Standard Biological Parts.
 +
                </li>
 +
                <li>Present a poster and a talk at the iGEM Jamboree. See the 2015 poster guidelines for
 +
                  more information.
 +
                </li>
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                <li>Create a page on your team wiki with clear attribution of each aspect of your
 +
                  project. This page must clearly attribute work done by the students and distinguish
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                  it from work done by others, including host labs, advisors, instructors, sponsors,
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                  professional website designers, artists, and commercial services.
 +
                </li>
 +
                <li>Develop a working prototype of an open-source hardware tool that supports synthetic
 +
                  biologists working with BioBrick standard biological parts. Document your tool on
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                  the project page on your team wiki.
 +
                </li>
 +
              </ul>
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              <h4 id="Silver" class="scrollspy">Silver:</h4>
  
                            <p><strong>SDS-PAGE</strong><br>After induced by IPTG in 1mM about 4 h, cheZ was found in
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              <p>In addition to the Bronze Medal requirements, your team must
                                SDS-PAGE gel, compared to the wild type (Top10) and bacteria without IPTG induction.
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                convince the judges you have achieved the following 3 goals:</p>
                                Through this result, we concluded that cheZ has been successfully expressed with the
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              <ul>
                                IPTG induction.<br><img
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                <li>Provide a demonstration of your hardware using a video tutorial that explains all
                                        src="https://static.igem.org/mediawiki/2015/7/72/Ustc-chez_expression.png" alt="图片名称"><br>See
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                  the features of the hardware operation. Make the video available on your wiki. You
                                protocol in <a href="http://tower.im">Protocols: SDS-PAGE</a></p>
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                  must also send the video file to iGEM HQ (email hq [at] igem [dot] org). <strong>See
                        </div>
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                    our tutorial video in <a href="http://tower.im">Tutorial-Instructional Video</a></strong>
                    </div>
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                </li>
                 </div>
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                <li>Document your device on your wiki with information and associated files sufficient
 +
                  for a future iGEM team to reproduce your work. You should include relevant design
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                  schematics, 3d print files (or other), software and a Bill of Materials (BOM).
 +
                  <strong>Want to reproduce our NDM? Please visit <a href="http://tower.im">Tutorial-Make
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                    your own</a></strong></li>
 +
                <li>iGEM projects involve important questions beyond the bench, for example relating to
 +
                  (but not limited to) ethics, sustainability, social justice, safety, security, and
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                  intellectual property rights. We refer to these activities as Human Practices in
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                  iGEM. Demonstrate how your team has identified, investigated and addressed one or
 +
                  more of these issues in the context of your project. (See the Human Practices Hub
 +
                  for more information.) <strong>See our Practices in <a href="http://tower.im">Policy&amp;Practices</a></strong>
 +
                </li>
 +
              </ul>
 +
              <h4 id="Gold" class="scrollspy">Gold:</h4>
 +
 
 +
              <p>In addition to the Bronze and Silver Medal requirements, your
 +
                team must convince the judges you have achieved at least two of the following goals:</p>
 +
              <ul>
 +
                <li>Choose one of these two options: (1) Expand on your silver medal Human Practices
 +
                  activity by demonstrating how you have integrated the investigated issues into the
 +
                  design and/or execution of your project. OR (2) Demonstrate an innovative Human
 +
                  Practices activity that relates to your project (this typically involves
 +
                  educational, public engagement, and/or public perception activities; see the Human
 +
                  Practices Hub for information and examples of innovative activities from previous
 +
                  teams).<strong>See our Integrated Practices Activities in <a href="http://tower.im">Policy&amp;Practices-Integrated
 +
                    Human Practice</a></strong></li>
 +
                <li>Help any other iGEM team utilize the hardware developed by your team. You must
 +
                  clearly show how your hardware was used and the results that were obtained. Results
 +
                  should take the form of a written report from the team testing the hardware and be
 +
                  posted on both team's wikis.
 +
                </li>
 +
                <li>Select a previous iGEM Hardware project from any track and demonstrate how you have
 +
                  continued to work on the project by improving the functionality, ease of use, or
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                  documentation of another facet of the work/device.
 +
                </li>
 +
                 <li>Demonstrate your hardware at the iGEM Giant Jamboree. If your equipment is too large
 +
                  to transport, create an installation to demonstrate functionality. Note: you may not
 +
                  bring live biological materials to the Jamboree.
 +
                </li>
 +
              </ul>
 
             </div>
 
             </div>
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          </div>
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          <div class="col hide-on-small-only m3">
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            <div class="toc-wrapper pinned">
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              <ul class="section table-of-contents">
 +
                <li>
 +
                  <a href="#Bronze">Bronze</a>
 +
                </li>
 +
                <li>
 +
                  <a href="#Silver">Silver</a>
 +
                </li>
 +
                <li>
 +
                  <a href="#Gold">Gold</a>
 +
                </li>
 +
              </ul>
 +
            </div>
 +
          </div>
 
         </div>
 
         </div>
        <div id="Parts" class="row">
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      </div>
            <div class="card hoverable">
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                            <table>
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                                <thead>
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                                <tr>
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                                    <th>Code Name</th>
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                                    <th>Parts Name</th>
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                                    <th>Type</th>
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                                    <th>Function</th>
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                                </tr>
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                                </thead>
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                                <tbody>
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                                <tr>
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                                    <td></td>
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                                    <td>micF</td>
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                                    <td>Simple/Promoter</td>
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                                    <td></td>
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                                </tr>
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                                <tr>
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                                    <td></td>
+
                                    <td>SoxS</td>
+
                                    <td>Simple/Promoter</td>
+
                                    <td></td>
+
                                </tr>
+
                                <tr>
+
                                    <td></td>
+
                                    <td>OprF</td>
+
                                    <td>Simple/CDS</td>
+
                                    <td></td>
+
                                </tr>
+
                                <tr>
+
                                    <td></td>
+
                                    <td>SCVE</td>
+
                                    <td>Simple/CDS</td>
+
                                    <td></td>
+
                                </tr>
+
                                <tr>
+
                                    <td></td>
+
                                    <td>cheZ</td>
+
                                    <td>Simple/CDS</td>
+
                                    <td></td>
+
                                </tr>
+
                                <tr>
+
                                    <td></td>
+
                                    <td>T7-RBS-SCVE</td>
+
                                    <td></td>
+
                                    <td></td>
+
                                </tr>
+
                                <tr>
+
                                    <td></td>
+
                                    <td>T7-RBS-OprF</td>
+
                                    <td></td>
+
                                    <td></td>
+
                                </tr>
+
                                <tr>
+
                                    <td></td>
+
                                    <td>R0010-RBS-cheZ</td>
+
                                    <td></td>
+
                                    <td></td>
+
                                </tr>
+
                                <tr>
+
                                    <td></td>
+
                                    <td>micF-RBS-GFP</td>
+
                                    <td></td>
+
                                    <td></td>
+
                                </tr>
+
                                <tr>
+
                                    <td></td>
+
                                    <td>SoxS-RBS-GFP</td>
+
                                    <td></td>
+
                                    <td></td>
+
                                </tr>
+
                                <tr>
+
                                    <td></td>
+
                                    <td>tRNA&amp;tRNA Synthetase</td>
+
                                    <td></td>
+
                                    <td></td>
+
                                </tr>
+
                                <tr>
+
                                    <td></td>
+
                                    <td>Compx</td>
+
                                    <td></td>
+
                                    <td></td>
+
                                </tr>
+
                                <tr>
+
                                    <td></td>
+
                                    <td>tRNA complex with Compx</td>
+
                                    <td></td>
+
                                    <td></td>
+
                                </tr>
+
                                </tbody>
+
                            </table>
+
                        </div>
+
                    </div>
+
  
                </div>
+
 
 +
    <div id="Future-NDM" class="row">
 +
      <div class="card hoverable">
 +
        <div class="row">
 +
          <div class="col s12 m9">
 +
            <div class="card-content">
 +
              <h4 id="what-will-our-ndm-look-like-in-future-" class="scrollspy">What will our NDM look like in future?</h4>
 +
            <p>This is the introduction of USTC's NDM future plan, we will briefly introduce our improvement on our system.</p>
 +
           
 +
 
 +
 
 +
            <h4 id="Transformation-of-CACCI" class="scrollspy">Transformation of CACCI</h4>
 +
            <p>In the future, CACCI will not just CACCI(that is characterization of antibiotics concentration based on chemotaxis and interference). Because of any possible molecules response promoter with integrated improvement in both chemotaxis and permeability. CACCI is able to detect anything in water as you wish. May we should substitute 'A' in CACCI in to '#', Let's say it <strong>C#CCI</strong>, or more academically, <strong>SBI, Standardized Biological Interface.</strong> As long as you get the bacteria powder you need, you can carry out experiments based on it or engineer it directly on it. My dear friend, isn't it cool?</p>
 +
            <p>SBI contains several modules: </p>
 +
            <ul>
 +
              <li>Integrated Improvement(IITwins). This is constructed by us, which is able to share after packaging SBI. See how to package bacteria: <a href="http://tower.im">Protocol: Package our CACCI</a>.</li>
 +
              <li>Editable Biological Circuit(EBC). We provided several possible signal sensing circuits with amplification or modification for you, accompanied with USTC-Software's project, you are able to generate your own genetic circuit used to response molecules you wanna detect. <strong>In the furture, NDM will be not just <em>Nanomachines Detecting Microbiotics</em>, but <em>Nanomachines Detecting Materials</em>. Everyone can edit them and operate them!</strong></li>
 +
              <li>Adhesion Preparation in Wide Adaptability(APWA). Two adhesion strategy is very widely adaptable for every user to construct new bacteria. Strategy I is based on polylysine coating, which doesn't need any genetic engineering. Though, Strategy II demands strict genetic modification, we are still able to share the bacteria with IITwins and tRNA plasmid in powder.</li>
 +
            </ul>
 +
            <p>In the future, SBI will be collected in a professional database. You could find your SBI in a more convenient and accurate way.</p>
 +
            <p>We could expect the day when SBI is finally established and widely used. Synthetic biology would not just share parts, those genetic modules packaged in plasmid, but share devices, different specific functional modules packaged in bacteria. We are looking forward to that moment.</p>
 +
           
 +
 
 +
 
 +
            <h4 id="More-Convenient-User-Experience" class="scrollspy">More Convenient User Experience</h4>
 +
            <ul>
 +
              <li>In the future, our GUI, that is graphic user interface, will finally be packaged in python, and it is for everyone. You can download it and install it for free. And now, GUI version in Matlab has already uploaded to Github, you can get them at: <a href="http://tower.im">Github-USTC</a>. We are very glad to share our ideas and get better.</li>
 +
              <li>An integrated circuit will be implemented into SPRING, which is able to strongly cut down the size of SPRING, though it has already convenient enough for people to detect molecules in field.</li>
 +
              <li>A more beautiful NDM: our designer will innovate several design plan to decorate our NDM. You could also decorate NDM by yourself. Come and join us!</li>
 +
            </ul>
 +
           
 +
 
 +
            <h4 id="Real-Assay" class="scrollspy">Real Assay</h4>
 +
            <ul>
 +
              <li>In the future, NDM will be used to detect microbiotics in river sample, which is the most severe situation now in China. As well, drinking water and milk may also be as detection object.</li>
 +
              <li>NDM will also be able to detect other contaminates in water samples, such as heavy metal ions, toxic organic compounds and other chemicals. With everyone's SBI, specific detection in 100s can be achieved.</li>
 +
            </ul>
 +
           
 +
 
 +
            <h4>We will then operate NDM in real sample, any suggestions on improvement of NDM, please contact us:)</h4>
 
             </div>
 
             </div>
        </div>
+
          </div>
        <div id="Medals" class="row">
+
          <div class="col hide-on-small-only m3">
             <div class="card hoverable">
+
             <div class="toc-wrapper pinned">
                <div class="row">
+
              <ul class="section table-of-contents">
                    <div class="col s12 m9">
+
                <li>
                        <div class="card-content">
+
                  <a href="#what-will-our-ndm-look-like-in-future-">What will our NDM look like in future?</a>
                            <p><strong>Bronze:</strong><br>Your team must convince the judges you have achieved the
+
                </li>
                                following 6 goals:</p>
+
                <li>
                            <ul>
+
                  <a href="#Transformation-of-CACCI">Transformation of CACCI</a>
                                <li>Register for iGEM, have a great summer, and attend the Giant Jamboree.</li>
+
                </li>
                                <li>Complete the Judging form.</li>
+
                <li>
                                <li>Create and share a Description of the team's project using the iGEM wiki, and
+
                  <a href="#More-Convenient-User-Experience">More Convenient User Experience</a>
                                    document the team's parts (if any) using the Registry of Standard Biological Parts.
+
                </li>
                                </li>
+
                <li>
                                <li>Present a poster and a talk at the iGEM Jamboree. See the 2015 poster guidelines for
+
                  <a href="#Real-Assay">Real Assay</a>
                                    more information.
+
                </li>
                                </li>
+
              </ul>
                                <li>Create a page on your team wiki with clear attribution of each aspect of your
+
                                    project. This page must clearly attribute work done by the students and distinguish
+
                                    it from work done by others, including host labs, advisors, instructors, sponsors,
+
                                    professional website designers, artists, and commercial services.
+
                                </li>
+
                                <li>Develop a working prototype of an open-source hardware tool that supports synthetic
+
                                    biologists working with BioBrick standard biological parts. Document your tool on
+
                                    the project page on your team wiki.
+
                                </li>
+
                            </ul>
+
                            <p><strong>Silver:</strong><br>In addition to the Bronze Medal requirements, your team must
+
                                convince the judges you have achieved the following 3 goals:</p>
+
                            <ul>
+
                                <li>Provide a demonstration of your hardware using a video tutorial that explains all
+
                                    the features of the hardware operation. Make the video available on your wiki. You
+
                                    must also send the video file to iGEM HQ (email hq [at] igem [dot] org). <strong>See
+
                                        our tutorial video in <a href="http://tower.im">Tutorial-Instructional Video</a></strong>
+
                                </li>
+
                                <li>Document your device on your wiki with information and associated files sufficient
+
                                    for a future iGEM team to reproduce your work. You should include relevant design
+
                                    schematics, 3d print files (or other), software and a Bill of Materials (BOM).
+
                                    <strong>Want to reproduce our NDM? Please visit <a href="http://tower.im">Tutorial-Make
+
                                        your own</a></strong></li>
+
                                <li>iGEM projects involve important questions beyond the bench, for example relating to
+
                                    (but not limited to) ethics, sustainability, social justice, safety, security, and
+
                                    intellectual property rights. We refer to these activities as Human Practices in
+
                                    iGEM. Demonstrate how your team has identified, investigated and addressed one or
+
                                    more of these issues in the context of your project. (See the Human Practices Hub
+
                                    for more information.) <strong>See our Practices in <a href="http://tower.im">Policy&amp;Practices</a></strong>
+
                                </li>
+
                            </ul>
+
                            <p><strong>Gold:</strong><br>In addition to the Bronze and Silver Medal requirements, your
+
                                team must convince the judges you have achieved at least two of the following goals:</p>
+
                            <ul>
+
                                <li>Choose one of these two options: (1) Expand on your silver medal Human Practices
+
                                    activity by demonstrating how you have integrated the investigated issues into the
+
                                    design and/or execution of your project. OR (2) Demonstrate an innovative Human
+
                                    Practices activity that relates to your project (this typically involves
+
                                    educational, public engagement, and/or public perception activities; see the Human
+
                                    Practices Hub for information and examples of innovative activities from previous
+
                                    teams).<strong>See our Integrated Practices Activities in <a href="http://tower.im">Policy&amp;Practices-Integrated
+
                                        Human Practice</a></strong></li>
+
                                <li>Help any other iGEM team utilize the hardware developed by your team. You must
+
                                    clearly show how your hardware was used and the results that were obtained. Results
+
                                    should take the form of a written report from the team testing the hardware and be
+
                                    posted on both team's wikis.
+
                                </li>
+
                                <li>Select a previous iGEM Hardware project from any track and demonstrate how you have
+
                                    continued to work on the project by improving the functionality, ease of use, or
+
                                    documentation of another facet of the work/device.
+
                                </li>
+
                                <li>Demonstrate your hardware at the iGEM Giant Jamboree. If your equipment is too large
+
                                    to transport, create an installation to demonstrate functionality. Note: you may not
+
                                    bring live biological materials to the Jamboree.
+
                                </li>
+
                            </ul>
+
                        </div>
+
                    </div>
+
                </div>
+
 
             </div>
 
             </div>
 +
          </div>
 
         </div>
 
         </div>
 +
      </div>
 
     </div>
 
     </div>
 +
 +
  </div>
 
</div>
 
</div>
 
</html>
 
</html>
 
{{USTC/footer}}
 
{{USTC/footer}}

Latest revision as of 03:17, 19 September 2015

These are what we have accomplished this year:

  • We successully constructed our CACCI, genetically modifed bacteria used to sense antibitoics.
  • We successfully achieved bacteria adhesing to film by treated with polylysine.
  • We successfully developed a device called SPRING with optical module and electric circuit.
  • We successfully proved adhesion dynamics would be stable after 100s.
  • We successfully established interference pattern by our SPRING.
  • We successfully proved that interference patterns have difference between each other when treating in different antibiotic concentration.
  • We successully calibrated our NDM for antibiotics determination.
  • We successfully developed a series of program used to process our interference pattern and data.
  • We successully demonstrated that our NDM is able to interact with bacteria, and distinguish different antibiotic concentration by delivering different interference pattern.

Bronze:

Your team must convince the judges you have achieved the following 6 goals:

  • Register for iGEM, have a great summer, and attend the Giant Jamboree.
  • Complete the Judging form.
  • Create and share a Description of the team's project using the iGEM wiki, and document the team's parts (if any) using the Registry of Standard Biological Parts.
  • Present a poster and a talk at the iGEM Jamboree. See the 2015 poster guidelines for more information.
  • Create a page on your team wiki with clear attribution of each aspect of your project. This page must clearly attribute work done by the students and distinguish it from work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.
  • Develop a working prototype of an open-source hardware tool that supports synthetic biologists working with BioBrick standard biological parts. Document your tool on the project page on your team wiki.

Silver:

In addition to the Bronze Medal requirements, your team must convince the judges you have achieved the following 3 goals:

  • Provide a demonstration of your hardware using a video tutorial that explains all the features of the hardware operation. Make the video available on your wiki. You must also send the video file to iGEM HQ (email hq [at] igem [dot] org). See our tutorial video in Tutorial-Instructional Video
  • Document your device on your wiki with information and associated files sufficient for a future iGEM team to reproduce your work. You should include relevant design schematics, 3d print files (or other), software and a Bill of Materials (BOM). Want to reproduce our NDM? Please visit Tutorial-Make your own
  • iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, and intellectual property rights. We refer to these activities as Human Practices in iGEM. Demonstrate how your team has identified, investigated and addressed one or more of these issues in the context of your project. (See the Human Practices Hub for more information.) See our Practices in Policy&Practices

Gold:

In addition to the Bronze and Silver Medal requirements, your team must convince the judges you have achieved at least two of the following goals:

  • Choose one of these two options: (1) Expand on your silver medal Human Practices activity by demonstrating how you have integrated the investigated issues into the design and/or execution of your project. OR (2) Demonstrate an innovative Human Practices activity that relates to your project (this typically involves educational, public engagement, and/or public perception activities; see the Human Practices Hub for information and examples of innovative activities from previous teams).See our Integrated Practices Activities in Policy&Practices-Integrated Human Practice
  • Help any other iGEM team utilize the hardware developed by your team. You must clearly show how your hardware was used and the results that were obtained. Results should take the form of a written report from the team testing the hardware and be posted on both team's wikis.
  • Select a previous iGEM Hardware project from any track and demonstrate how you have continued to work on the project by improving the functionality, ease of use, or documentation of another facet of the work/device.
  • Demonstrate your hardware at the iGEM Giant Jamboree. If your equipment is too large to transport, create an installation to demonstrate functionality. Note: you may not bring live biological materials to the Jamboree.

What will our NDM look like in future?

This is the introduction of USTC's NDM future plan, we will briefly introduce our improvement on our system.

Transformation of CACCI

In the future, CACCI will not just CACCI(that is characterization of antibiotics concentration based on chemotaxis and interference). Because of any possible molecules response promoter with integrated improvement in both chemotaxis and permeability. CACCI is able to detect anything in water as you wish. May we should substitute 'A' in CACCI in to '#', Let's say it C#CCI, or more academically, SBI, Standardized Biological Interface. As long as you get the bacteria powder you need, you can carry out experiments based on it or engineer it directly on it. My dear friend, isn't it cool?

SBI contains several modules:

  • Integrated Improvement(IITwins). This is constructed by us, which is able to share after packaging SBI. See how to package bacteria: Protocol: Package our CACCI.
  • Editable Biological Circuit(EBC). We provided several possible signal sensing circuits with amplification or modification for you, accompanied with USTC-Software's project, you are able to generate your own genetic circuit used to response molecules you wanna detect. In the furture, NDM will be not just Nanomachines Detecting Microbiotics, but Nanomachines Detecting Materials. Everyone can edit them and operate them!
  • Adhesion Preparation in Wide Adaptability(APWA). Two adhesion strategy is very widely adaptable for every user to construct new bacteria. Strategy I is based on polylysine coating, which doesn't need any genetic engineering. Though, Strategy II demands strict genetic modification, we are still able to share the bacteria with IITwins and tRNA plasmid in powder.

In the future, SBI will be collected in a professional database. You could find your SBI in a more convenient and accurate way.

We could expect the day when SBI is finally established and widely used. Synthetic biology would not just share parts, those genetic modules packaged in plasmid, but share devices, different specific functional modules packaged in bacteria. We are looking forward to that moment.

More Convenient User Experience

  • In the future, our GUI, that is graphic user interface, will finally be packaged in python, and it is for everyone. You can download it and install it for free. And now, GUI version in Matlab has already uploaded to Github, you can get them at: Github-USTC. We are very glad to share our ideas and get better.
  • An integrated circuit will be implemented into SPRING, which is able to strongly cut down the size of SPRING, though it has already convenient enough for people to detect molecules in field.
  • A more beautiful NDM: our designer will innovate several design plan to decorate our NDM. You could also decorate NDM by yourself. Come and join us!

Real Assay

  • In the future, NDM will be used to detect microbiotics in river sample, which is the most severe situation now in China. As well, drinking water and milk may also be as detection object.
  • NDM will also be able to detect other contaminates in water samples, such as heavy metal ions, toxic organic compounds and other chemicals. With everyone's SBI, specific detection in 100s can be achieved.

We will then operate NDM in real sample, any suggestions on improvement of NDM, please contact us:)

Contact Us

University of Science and Technology of China, No.96, JinZhai Road Baohe District,Hefei,Anhui, 230026,P.R.China.

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